全文获取类型
收费全文 | 6167篇 |
免费 | 374篇 |
国内免费 | 45篇 |
专业分类
耳鼻咽喉 | 29篇 |
儿科学 | 162篇 |
妇产科学 | 168篇 |
基础医学 | 1013篇 |
口腔科学 | 80篇 |
临床医学 | 503篇 |
内科学 | 1635篇 |
皮肤病学 | 306篇 |
神经病学 | 583篇 |
特种医学 | 143篇 |
外科学 | 452篇 |
综合类 | 11篇 |
一般理论 | 2篇 |
预防医学 | 357篇 |
眼科学 | 37篇 |
药学 | 377篇 |
中国医学 | 26篇 |
肿瘤学 | 702篇 |
出版年
2024年 | 6篇 |
2023年 | 54篇 |
2022年 | 148篇 |
2021年 | 209篇 |
2020年 | 118篇 |
2019年 | 175篇 |
2018年 | 201篇 |
2017年 | 131篇 |
2016年 | 151篇 |
2015年 | 204篇 |
2014年 | 257篇 |
2013年 | 304篇 |
2012年 | 468篇 |
2011年 | 533篇 |
2010年 | 291篇 |
2009年 | 241篇 |
2008年 | 400篇 |
2007年 | 389篇 |
2006年 | 427篇 |
2005年 | 374篇 |
2004年 | 350篇 |
2003年 | 305篇 |
2002年 | 291篇 |
2001年 | 36篇 |
2000年 | 32篇 |
1999年 | 58篇 |
1998年 | 62篇 |
1997年 | 59篇 |
1996年 | 49篇 |
1995年 | 46篇 |
1994年 | 34篇 |
1993年 | 33篇 |
1992年 | 19篇 |
1991年 | 19篇 |
1990年 | 15篇 |
1989年 | 24篇 |
1988年 | 16篇 |
1987年 | 8篇 |
1986年 | 9篇 |
1985年 | 4篇 |
1984年 | 11篇 |
1983年 | 4篇 |
1982年 | 1篇 |
1981年 | 5篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1977年 | 5篇 |
1975年 | 2篇 |
1972年 | 2篇 |
排序方式: 共有6586条查询结果,搜索用时 15 毫秒
21.
Candida albicans yeast and germ tube forms interfere differently with human monocyte differentiation into dendritic cells: a novel dimorphism-dependent mechanism to escape the host's immune response 下载免费PDF全文
22.
Protection of killer antiidiotypic antibodies against early invasive aspergillosis in a murine model of allogeneic T-cell-depleted bone marrow transplantation 总被引:5,自引:0,他引:5 下载免费PDF全文
Cenci E Mencacci A Spreca A Montagnoli C Bacci A Perruccio K Velardi A Magliani W Conti S Polonelli L Romani L 《Infection and immunity》2002,70(5):2375-2382
Antiidiotypic monoclonal antibodies (MAbs) representing the internal image of a yeast killer toxin (KT) have therapeutic potential against several fungal infections. The efficacy of KT MAbs against Aspergillus fumigatus was investigated in a mouse model of T-cell-depleted allogeneic bone marrow transplantation (BMT) with invasive pulmonary aspergillosis. Mice were highly susceptible to infection at 3 days post-BMT, when profound neutropenia was observed both in the periphery and in the lungs. Treatment with KT MAbs protected the mice from infection, as judged by the long-term survival and decreased pathology associated with inhibition of fungal growth and hyphal development in the lungs. In vitro, similar to polymorphonuclear neutrophils, KT MAbs significantly inhibited the hyphal development and metabolic activity of germinated Aspergillus conidia. These results indicate that mimicking the action of neutrophils could be a strategy through which KT MAbs exert therapeutic efficacy in A. fumigatus infections. 相似文献
23.
Mapping antigenic sites of an immunodominant surface lipoprotein of Mycoplasma agalactiae, AvgC, with the use of synthetic peptides 下载免费PDF全文
As a first step toward the design of an epitope vaccine to prevent contagious agalactia, the strongly immunogenic 55-kDa protein of Mycoplasma agalactiae was studied and found to correspond to the AvgC protein encoded by the avgC gene. The avg genes of M. agalactiae, which encode four variable surface lipoproteins, display a significant homology to the vsp (variable membrane surface lipoproteins) genes of the bovine pathogen Mycoplasma bovis at their promoter region as well as their N-terminus-encoding regions. Some members of the Vsp family are known to be involved in cytoadhesion to host cells. In order to localize immunogenic peptides in the AvgC antigen, the protein sequence was submitted to epitope prediction analysis, and five sets of overlapping peptides, corresponding to five selected regions, were synthesized by Spot synthesis. Reactive peptides were selected by immunobinding assay with sera from infected sheep. The three most immunogenic epitopes were shown to be surface exposed by immunoprecipitation assays, and one of these was specifically recognized by all tested sera. Our study indicates that selected epitopes of the AvgC lipoprotein may be used to develop a peptide-based vaccine which is effective against M. agalactiae infection. 相似文献
24.
Calorini L Bianchini F Mannini A Mugnai G Balzi M Becciolini A Ruggieri S 《Clinical & experimental metastasis》2002,19(3):259-264
In the present study, we found that murine peritoneal macrophages elicited by BCG or Listeria monocytogenes release into the media an activity capable of stimulating the lung colonization as well as the expression of MHC class I
antigens in B16 melanoma cells. A similar activity has previously been found in media conditioned by Corynebacterium parvum-elicited macrophages. Analysis by gel filtration chromatography of media conditioned by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages revealed that the material responsible for the pro-clonogenic activity concentrated in chromatographic
fractions corresponding to molecular weights (25 to 52 kDa) which are characteristic of certain cytokines. Thus, we challenged
the various macrophage-conditioned media with polyclonal antibodies against IFNγand TNFα, and found that the macrophage pro-clonogenic
activity was completely abolished in the presence of anti-IFNγantibodies, but only partially inhibited by anti-TNFαantibodies.
This finding suggests a cooperative participation of the two cytokines to the pro-clonogenic activity of the media conditioned
by Corynebacterium parvum-, BCG- or Listeria monocytogenes-elicited macrophages.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
25.
Dettin M Conconi MT Gambaretto R Pasquato A Folin M Di Bello C Parnigotto PP 《Journal of biomedical materials research》2002,60(3):466-471
Next generation dental/orthopedic biomaterials must be designed to enhance and support osteoblast adhesion. The osteoblasts use different ways to adhere, that is, integrin- and proteoglycan-mediated mechanisms. The present study reports on the synthesis and osteoblast-adhesive properties of peptides carrying RGD motifs and of sequences mapped on human vitronectin. Our data suggest that osteoblast adhesion on polystyrene plates modified with a linear peptide, in which the GRGDSP sequence is repeated four times, was significantly higher when compared to the adhesion obtained using branched peptides, interestingly containing the same motif. Osteoblast adhesion assays on acellular bone matrix using this active peptide gave very promising results. We also demonstrated that a novel peptide, carrying the X-B-B-B-X-B-B-X motif (where B is a basic amino acid and X is a nonbasic residue), promotes proteoglycan-mediated osteoblast adhesion more efficiently with respect to the KRSR sequence that was recently proposed as heparan-sulfate binding peptide. 相似文献
26.
27.
Idiopathic CD4+ lymphocytopenia may be due to decreased bone marrow clonogenic capability 总被引:1,自引:0,他引:1
Isgrò A Sirianni MC Gramiccioni C Mezzaroma I Fantauzzi A Aiuti F 《International archives of allergy and immunology》2005,136(4):379-384
BACKGROUND: Idiopathic CD4+ lymphocytopenia is defined by a stable decrease of CD4+ T cells in the absence of any known cause of immune deficiency. The mechanisms responsible for the immunological impairment are still unknown, but a regenerative failure of hematopoietic stem/progenitor cells has been hypothesized. METHODS: We evaluated in the bone marrow (BM) of 5 patients with idiopathic CD4+ lymphocytopenia the phenotype of BM progenitor cells, their differentiation capacity with colony-forming cells and long-term culture-initiating cell assays, in parallel with the spontaneous IL-7 production in the patient sera. RESULTS: Compared with controls, a regenerative failure of hematopoietic stem cells has been observed, both in 'committed' and in 'uncommitted' progenitor cells, despite high IL-7 serum levels. The percentage of phenotypically primitive CD34+CD38-DR+ cells (this includes the lymphoid precursor cells) was decreased, suggesting an involvement of the more primitive BM compartment in the de novo T cell generation. CONCLUSIONS: Despite the low number of patients, due to the low incidence of the disease, the decrease of primitive precursors sustains the possibility that diminished stem cell precursors might contribute to the development of CD4+ T cell depletion. 相似文献
28.
Antonella Cianferoni Elio Novembre Neri Pucci Enrico Lombardi Roberto Bernardini Alberto Vierucci 《Annals of allergy, asthma & immunology》2004,92(4):464-468
BACKGROUND: Little is known about the frequency of and the features associated with recurrent anaphylaxis in pediatric populations. During 1994 to 1996, we enrolled 76 children affected by anaphylaxis in a prospective study to analyze their clinical and allergic features. OBJECTIVE: To undertake a follow-up study of these children to ascertain how many experienced a recurrence of anaphylaxis. METHODS: After a mean interval of 7 years, a pediatric allergist conducted a telephone interview of patients who had been enrolled in our 1994-1996 study. RESULTS: A telephone interview was successfully completed in 46 (61%) of the 76 patients who had been enrolled in our 1994-1996 study. Of these 46 patients, 14 (30%) had experienced a recurrence of anaphylaxis. Children with atopic dermatitis either during 1994 to 1996 (64% vs 34%; P = .04) or at the time of the current study (43% vs 16%; P = .03) and those with urticaria-angioedema at the time of the current study (93% vs 31%; P = .0002) were found to be at a significantly higher risk for recurrent anaphylaxis. Furthermore, those children who were sensitive to at least 1 food allergen during 1994 to 1996 were more likely to have experienced a recurrence of anaphylaxis (93% vs 56%; P < .04). CONCLUSIONS: This study suggests that patients may have a greater risk of recurrence of anaphylaxis if they have atopic dermatitis, urticaria-angioedema, or at least 1 positive result of skin prick tests to food allergens. 相似文献
29.
Zei G Lisa A Fiorani O Magri C Quintana-Murci L Semino O Santachiara-Benerecetti AS 《European journal of human genetics : EJHG》2003,11(10):802-807
A total of 202 Sardinian male subjects were examined for 13 biallelic stable markers, the complex 49a,f/TaqI system and three microsatellites of the Y chromosome in order to investigate, through surname analysis, on a possible territorial heterogeneity inside the island. The study of geographical distribution and linguistic derivation of Sardinian surnames allow us to discover their 'probable place of origin' and reconstruct ancient genetic isolates which borders are, today, no more recognizable. The molecular analysis revealed that about 90% of the Sardinian Y chromosomes fell into haplogroups E-M35, G-M201, I-M26, J-12f2 and R-M269. In contrast with the territorial homogeneity of these haplogroups, when the individuals were distributed according to their birthplace, a significant difference between the three historically and culturally distinct geographical areas into which Sardinia can be subdivided was observed when the individuals were distributed according to the ancestral location of surnames. In particular, the major contribution to this heterogeneity is due to the 'Sardinian-specific' haplogroup I-M26 (almost completely associated with the 49a,f-Ht12/12f2-10Kb/YCAIIa-21/YCAIIb-11 compound haplotype), which shows both a significantly higher incidence in the central-eastern (archaic) area and a significantly lower frequency in the northern area. The results of this study agree with the hypothesis that the ancestral homeland of this specific subset of haplogroup I is the mountainous central-eastern area of Sardinia, where the population underwent a long history of isolation since ancient times, and highlight the informative power of the surname analysis. 相似文献
30.
Johann Kaspar Lieberwirth Pascal Joset Anja Heinze Julia Hentschel Anja Stein Antonella Iannaccone Katharina Steindl Alma Kuechler Rami Abou Jamra 《European journal of human genetics : EJHG》2021,29(5):808
Perinatal mortality is a heavy burden for both affected parents and physicians. However, the underlying genetic causes have not been sufficiently investigated and most cases remain without diagnosis. This impedes appropriate counseling or therapy. We describe four affected children of two unrelated families with cardiomyopathy, hydrops fetalis, or cystic hygroma that all deceased perinatally. In the four patients, we found the following homozygous loss of function (LoF) variants in SLC30A5 NM_022902.4:c.832_836del p.(Ile278Phefs*33) and NM_022902.4:c.1981_1982del p.(His661Tyrfs*10). Knockout of SLC30A5 has previously been shown a cardiac phenotype in mouse models and no homozygous LoF variants in SLC30A5 are currently described in gnomAD. Taken together, we present SLC30A5 as a new gene for a severe and perinatally lethal form of cardiomyopathy.Subject terms: Cardiovascular diseases, Development, Medical genetics, Medical genomics 相似文献