全文获取类型
收费全文 | 2493篇 |
免费 | 188篇 |
国内免费 | 18篇 |
专业分类
耳鼻咽喉 | 23篇 |
儿科学 | 61篇 |
妇产科学 | 88篇 |
基础医学 | 397篇 |
口腔科学 | 63篇 |
临床医学 | 294篇 |
内科学 | 534篇 |
皮肤病学 | 85篇 |
神经病学 | 230篇 |
特种医学 | 56篇 |
外科学 | 213篇 |
综合类 | 6篇 |
一般理论 | 2篇 |
预防医学 | 235篇 |
眼科学 | 11篇 |
药学 | 148篇 |
中国医学 | 2篇 |
肿瘤学 | 251篇 |
出版年
2024年 | 4篇 |
2023年 | 37篇 |
2022年 | 59篇 |
2021年 | 108篇 |
2020年 | 71篇 |
2019年 | 85篇 |
2018年 | 114篇 |
2017年 | 88篇 |
2016年 | 96篇 |
2015年 | 95篇 |
2014年 | 111篇 |
2013年 | 152篇 |
2012年 | 201篇 |
2011年 | 188篇 |
2010年 | 114篇 |
2009年 | 104篇 |
2008年 | 141篇 |
2007年 | 161篇 |
2006年 | 140篇 |
2005年 | 147篇 |
2004年 | 123篇 |
2003年 | 101篇 |
2002年 | 68篇 |
2001年 | 22篇 |
2000年 | 12篇 |
1999年 | 13篇 |
1998年 | 23篇 |
1997年 | 18篇 |
1996年 | 8篇 |
1995年 | 17篇 |
1994年 | 22篇 |
1993年 | 7篇 |
1992年 | 5篇 |
1991年 | 4篇 |
1990年 | 5篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 6篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有2699条查询结果,搜索用时 0 毫秒
71.
Svensson AM Curry CJ South ST Whitby H Maxwell TM Aston E Fisher J Carmack CE Scheffer A Abu-Shamsieh A Brothman AR 《American journal of medical genetics. Part A》2007,(12):1348-1353
We describe the cytogenetic diagnosis using BAC- and oligonucleotide microarrays of a 16-year-old Laotian-American female, who first presented at 2 1/2 years of age with microcephaly, developmental retardation, and skeletal abnormalities of the upper limb including mild syndactyly of the second and third and the third and fourth fingers, short middle phalanges and clinodactyly of the fifth digit at the distal interphalangel joint on both hands, and symphalangism of the metacarpal-phalangeal joints of the second and fifth digits bilaterally. Her lower limbs displayed symphalangism of the metatarsal-phalangeal joint of the second, third, and fourth digits on both feet, with fusion of the middle and distal phalanges of the second and fifth digits and hallux valgus bilaterally. G-banded chromosomal study at age 4 was normal. However, comparative genomic hybridization at age 15 with the Spectral Genomics 1 Mb Hu BAC array platform indicated a microdeletion involving two BAC clones, RP11-451F14 --> RP11-12N7 at 2q31.1. The maximal deletion on initial analysis comprised the HOXD cluster, which is implicated in limb development. Fluorescence in situ hybridization (FISH) using the RP11-451F14 probe confirmed the deletion. Both parents were negative for the deletion. Additional FISH using BAC RP11-387A1, covering the HOXD cluster, limited the maximal deletion to approximately 2.518 Mb, and excluded involvement of the HOXD cluster. The Agilent 44K and 244K platforms demonstrated a deletion of approximately 2,011,000 bp, which did not include the HOXD cluster. The malformations in our patient may be caused by deletion of a regulatory element far upstream of the HOXD cluster. 相似文献
72.
Sarah E. Maylott Annika Paukner Yeojin A. Ahn Elizabeth A. Simpson 《Developmental psychobiology》2020,62(6):841-857
The present study explored behavioral norms for infant social attention in typically developing human and nonhuman primate infants. We examined the normative development of attention to dynamic social and nonsocial stimuli longitudinally in macaques (Macaca mulatta) at 1, 3, and 5 months of age (N = 75) and humans at 2, 4, 6, 8, and 13 months of age (N = 69) using eye tracking. All infants viewed concurrently played silent videos—one social video and one nonsocial video. Both macaque and human infants were faster to look to the social than the nonsocial stimulus, and both species grew faster to orient to the social stimulus with age. Further, macaque infants’ social attention increased linearly from 1 to 5 months. In contrast, human infants displayed a nonlinear pattern of social interest, with initially greater attention to the social stimulus, followed by a period of greater interest in the nonsocial stimulus, and then a rise in social interest from 6 to 13 months. Overall, human infants looked longer than macaque infants, suggesting humans have more sustained attention in the first year of life. These findings highlight potential species similarities and differences, and reflect a first step in establishing baseline patterns of early social attention development. 相似文献
73.
74.
Telomerase Repeat Amplification Protocol (TRAP) in situ was performed on cytospin preparations from 65 effusions from the serous cavities (45 pleural and 19 ascitic fluids and one pericardial fluid) submitted for routine diagnosis and the results were correlated to cytological morphology. Three types of cells with nuclear fluorescence were identified: malignant cells, hyperplastic mesothelial cell and lymphocytes. Of 38 cytologically malignant effusions, 12 showed strong reactivity in all malignant cells, three strong reactivity in part of the malignant population, whereas 12 showed moderate reactivity in the whole and five in part of the malignant population, respectively. In five malignant effusions weak reactivity was found in all (one case) and in scattered (four cases) malignant cells. Two effusions contained telomerase-negative malignant cells. Two pleural and two ascitic fluids contained proliferative mesothelial cells with weak or, in one case, moderate reactivity. Lymphocytes usually showed weak telomerase activity. Telomerase was expressed in almost all malignant tumours metastatic to serous cavities. Heterogeneity in tumour populations was demonstrated, which may have diagnostic implications, especially in cytology. Weak or moderate reactivity was found in lymphocytes and in some mesothelial proliferations and may explain the low specificity for malignancy sometimes obtained with the TRAP extract method. The weak reactivity found in lymphocytes may reduce the specificity when the extract method is used but causes no diagnostic problem with the TRAP in situ method. 相似文献
75.
Vendelin J Pulkkinen V Rehn M Pirskanen A Räisänen-Sokolowski A Laitinen A Laitinen LA Kere J Laitinen T 《American journal of respiratory cell and molecular biology》2005,33(3):262-270
We recently identified a novel positional asthma susceptibility gene, GPRA, which belongs to the G protein-coupled receptor family. In the present studies, we show that isoform specific activation of GPRA-A with its agonist, Neuropeptide S (NPS) resulted in significant inhibition of cell growth. GPRA has several variants due to extensive alternative splicing. We observed that only the full-length variants, GPRA-A and GPRA-B, with 7 transmembrane topology are transported into the plasma membrane, while the truncated proteins retain intracellular compartments. To clarify disease mechanism, we studied co-expression of the variants without finding any indication that truncated variants would inhibit the receptor transport into the plasma membrane. By using in situ hybridization and immunohistochemistry, we detected ubiquitous expression of GPRA-B, and frequent expression of GPRA-A in the epithelia of several organs including bronchi and gastrointestinal tract. Furthermore, we observed aberrant mRNA and protein expression levels of GPRA in the asthmatic bronchi. Finally, we demonstrate that GPRA and NPS are co-expressed in bronchial epithelium. In summary, this study provides evidence that GPRA might have functional relevance in modulating asthma by increased expression levels in the relevant tissues under diseased state and by potential inhibitory effect of GPRA-A activation on cell growth. 相似文献
76.
Haemophilus ducreyi causes genital ulceration (chancroid), a sexually transmitted infection and still an important factor which contributes to the spread of HIV in developing countries. The bacterium produces a cytolethal distending toxin (HdCDT) causing cell cycle arrest and apoptosis/necrosis of human cells and contributes to the aggravation of ulcers. The aim of the study was to induce toxin-neutralizing antibodies in the genital tract of mice. Repeated subcutaneous (sc) immunisations with 5–10 μg active HdCDT induced low levels of serum anti-HdCDT IgG without neutralizing capacity. High levels of specific IgG1 antibodies in serum and genital tract were generated after sc immunisations with 10 μg formaldehyde detoxified HdCDT toxoid alone and the addition of aluminium salts or RIBI (based on the lipid A moiety) as adjuvant further increased the level of serum antibodies. A high correlation was found between elevated levels of anti-HdCDT IgG in sera, the level of neutralizing activity and the antibody level in genital tract (r = 0.8). Thus, induction of high antibody levels specific to HdCDT in the genital tissue can be achieved by parenteral immunisation with the toxoid. The HdCDT toxoid can be considered as a candidate component in vaccine against chancroid. 相似文献
77.
Paurnima Patil Agata Cieslak Stephan H. Bernhart Umut H. Toprak Rabea Wagener Cristina Lpez Laura Wiehle Susanne Bens Janine Altmüller Marek Franitza Ingrid Scholz Sandrine Jayne Matthew J. Ahearne Annika Scheffold Billy M. C. Jebaraj Christof Schneider Dolors Costa Till Braun Alexandra Schrader Elias Campo Martin J. S. Dyer Peter Nürnberg Jan Dürig Patricia Johansson Sebastian Bttcher Matthias Schlesner Marco Herling Stephan Stilgenbauer Elizabeth Macintyre Reiner Siebert 《Genes, chromosomes & cancer》2020,59(4):261-267
78.
Khuloud Bajbouj Angela Poehlmann Doerthe Kuester Thomas Drewes Kathrin Haase Roland Hartig Anne Teller Stefanie Kliche Diana Walluscheck Jelena Ivanovska Saritha Chakilam Annika Ulitzsch Ursula Bommhardt Martin Leverkus Albert Roessner Regine Schneider-Stock 《The American journal of pathology》2009,175(2):557-570
79.
Vilse, a conserved Rac/Cdc42 GAP mediating Robo repulsion in tracheal cells and axons 总被引:3,自引:0,他引:3
Lundström A Gallio M Englund C Steneberg P Hemphälä J Aspenström P Keleman K Falileeva L Dickson BJ Samakovlis C 《Genes & development》2004,18(17):2161-2171
Slit proteins steer the migration of many cell types through their binding to Robo receptors, but how Robo controls cell motility is not clear. We describe the functional analysis of vilse, a Drosophila gene required for Robo repulsion in epithelial cells and axons. Vilse defines a conserved family of RhoGAPs (Rho GTPase-activating proteins), with representatives in flies and vertebrates. The phenotypes of vilse mutants resemble the tracheal and axonal phenotypes of Slit and Robo mutants at the CNS midline. Dosage-sensitive genetic interactions between vilse, slit, and robo mutants suggest that vilse is a component of robo signaling. Moreover, overexpression of Vilse in the trachea of robo mutants ameliorates the phenotypes of robo, indicating that Vilse acts downstream of Robo to mediate midline repulsion. Vilse and its human homolog bind directly to the intracellular domains of the corresponding Robo receptors and promote the hydrolysis of RacGTP and, less efficiently, of Cdc42GTP. These results together with genetic interaction experiments with robo, vilse, and rac mutants suggest a mechanism whereby Robo repulsion is mediated by the localized inactivation of Rac through Vilse. 相似文献
80.
Elevated levels of IgG and IgG4 to Malassezia allergens in atopic eczema patients with IgE reactivity to Malassezia 总被引:2,自引:0,他引:2
Johansson C Tengvall Linder M Aalberse RC Scheynius A 《International archives of allergy and immunology》2004,135(2):93-100
BACKGROUND: The opportunistic yeast Malassezia is considered to be one of the factors that can contribute to atopic eczema (AE). Elevated serum IgE levels, T-cell proliferation and positive skin prick test (SPT) and atopy patch test (APT) reactions to Malassezia are found among AE patients. METHODS: Sera from 127 AE patients, 14 patients with seborrheic dermatitis (SD) and 33 healthy controls were investigated for IgE and IgG4 to M. sympodialis extract and four recombinant Malassezia allergens; rMala s 1, rMala s 5, rMala s 6, and rMala s 9. In addition, IgG to the recombinant allergens was analyzed. The IgG and IgG4 levels were compared to IgE levels and in vivo reactions (SPT and APT) to Malassezia. RESULTS: AE patients with serum IgE levels >0.35 kU/l to M. sympodialis extract had significantly higher IgG4 levels to M. sympodialis extract than AE patients without detectable serum IgE to M. sympodialis extract, SD patients and healthy controls. Among the AE patients with and without detectable serum IgE to M. sympodialis extract, respectively, there were no differences in IgG4 levels between patients with positive or negative in vivo reactions to M. sympodialis extract. IgG4 to the rMala s allergens was almost exclusively found among patients with IgE to the same allergen. Within the four tested rMala s allergens, most IgG4 reactions were found to rMala s 6, an allergen with homology to cyclophilin. CONCLUSIONS: Elevated serum IgG4 to M. sympodialis extract accompanies elevated serum IgE to the extract. This is further confirmed by the association between IgG/IgG4 and IgE to recombinant Malassezia allergens. 相似文献