Investigation of micronuclei and other nuclear abnormalities in peripheral blood and kidney of marine fish treated with crude oil

The induction of micronuclei and other nuclear abnormalities (nuclear buds, bi-nucleated and fragmented-apoptotic cells) was analyzed in the erythrocytes of peripheral blood and cephalic kidney of turbot (Scophthalmus maximus) and Atlantic cod (Gadus morua), treated with crude oil (Statfjord B, Norway) and with nonylphenol. Significant increase in MN was observed in turbot kidney and blood after exposure to 30 ppb of nonylphenol, 0.5 ppm of oil, and after co-exposure to 0.5 ppm of oil spiked with additional mixture of alkylphenols and PAHs (P varied between 0.0054 and <0.0001). The induction of micronuclei was observed only in cod kidney after exposure to spiked oil (P=0.0317). Significant inter-specific differences after the exposure to 0.5 ppm of oil (P=0.0385) and after treatment with spiked oil (P=0.0067) were observed. In turbot cephalic kidney, the elevated levels of bi-nucleated cells were observed in all treatment groups (P values varied in a range from 0.05 to 0.0025) while the increase in cells with nuclear buds was noted after the exposure to 0.5 ppm of oil (P=0.05). The fragmented-apoptotic cells appeared after the exposure to nonylphenol (P=0.0039) and to spiked oil (P<0.0001). In turbot blood, only the significant induction in nuclear buds was detected. Statistically significant inter-tissue differences were found only in the induction of fragmented-apoptotic cells after the exposure to nonylphenol and to spiked oil.     

Computer-assisted two-dimensional echocardiography in preoperative evaluation of left ventricular function in patients with aortic valve disease

Seventy-six healthy adults and 228 patients with aortic valve disease were studied by means of two-dimensional echocardiography (2D-EchoCG). Functional properties of the left ventricle in patients with aortic valve disease were studied by means of computer analysis of echocardiograms and patients requiring cardiac surgery were identified. It was found that an increased left ventricular (LV) end-systolic volume (110 ml and more), assessed by 2D-EchoCG, is an unfavourable prognostic sign, and elevated risk factor of cardiac surgery. It invariably proved to be related to LV dysfunction after the surgery. A decreased diastolic LV mass/volume ratio (1.255 and less), found in 16% of patients with pressure overload, means nonadequate development of hypertrophy and an elevated risk of cardiac surgery.     

Therapy of refractory ventricular tachycardia by transvenous electrical ablation

In 6 patients (mean age 49 +/- 11 years) with chronic recurrent ventricular tachycardia which were relatively slow during antiarrhythmic therapy and had been proven to be resistant to various antiarrhythmic drugs, catheter ablation of the site of origin of ventricular tachycardia was performed after endocardial activation and/or pace-mapping. According to the results of catheter mapping, the sites of origin of ventricular tachycardia were in the region of the infero-lateral wall (n = 3), in the anterolateral wall (n = 1) and in the right ventricle (n = 2). In 3 cases, two ablative procedures were performed as the first was only transiently successful. A total of 51 shocks was delivered (200 J 45 times, 100 J 5 times and 400 J once). The following complications were observed: multiple episodes of spontaneous, partly polymorphous ventricular tachycardia which degenerated into ventricular flutter on several episodes (n = 1); transient third degree AV-block and intermittent complete right bundle branch block (n = 1); transient ST-elevation (n = 3); and self-terminating atrial tachycardia lasting for several minutes (n = 1). Immediately after the first ablative procedure, either no ventricular tachycardia could be induced any longer (n = 2) or non-clinical ventricular tachycardia was induced (n = 2). In the remaining 2 patients, programmed ventricular stimulation was not repeated immediately at the end of the ablative procedure. During a follow-up study at the end of the first week, the clinical ventricular tachycardia could be induced again in 3 of 4 cases. In 3 cases, a second ablative procedure was performed because of recurrences of ventricular tachycardia (n = 2) or syncope (n = 1). During final follow-up (mean 4.3 +/- 3.4 months), no recurrences of ventricular tachycardia were observed in 5 cases. In one case, recurrences occurred. Therefore, an automatic cardioverter was implanted. In conclusion, these preliminary results show that catheter ablation of drug-resistant ventricular tachycardia is feasible and may be an alternative to surgical procedures.