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11.
Analysis of the genetic determinants coding for the S-fimbrial adhesin (sfa) in different Escherichia coli strains causing meningitis or urinary tract infections. 总被引:4,自引:10,他引:4 下载免费PDF全文
Recently we have described the molecular cloning of the genetic determinant coding for the S-fimbrial adhesin (Sfa), a sialic acid-recognizing pilus frequently found among extraintestinal Escherichia coli isolates. Fimbriae from the resulting Sfa+ E. coli K-12 clone were isolated, and an Sfa-specific antiserum was prepared. Western blots indicate that S fimbriae isolated from different uropathogenic and meningitis-associated E. coli strains, including O83:K1 isolates, were serologically related. The Sfa-specific antibodies did not cross-react with P fimbriae, but did cross-react with F1C fimbriae. Furthermore the sfa+ recombinant DNAs and some cloned sfa-flanking regions were used as probes in Southern experiments. Chromosomal DNAs isolated from O18:K1 and O83:K1 meningitis strains with and without S fimbriae and from uropathogenic O6:K+ strains were hybridized against these sfa-specific probes. Only one copy of the sfa determinant was identified on the chromosome of these strains. No sfa-specific sequences were observed on the chromosome of E. coli K-12 strains and an O7:K1 isolate. With the exception of small alterations in the sfa-coding region the genetic determinants for S fimbriae were identical in uropathogenic O6:K+ and meningitis O18:K1 and O83:K1 strains. The sfa determinant was also detected on the chromosome of K1 isolates with an Sfa-negative phenotype, and specific cross-hybridization signals were visible after blotting against F1C-specific DNA. In addition homology among the different strains was observed in the sfa-flanking regions. 相似文献
12.
Identification of factors in human urine that inhibit the binding of Escherichia coli adhesins. 总被引:4,自引:5,他引:4 下载免费PDF全文
Earlier studies on the binding of Escherichia coli adhesins to the human urinary tract have indicated that the ability to recognize binding sites on the urinary tract epithelial cells is not a characteristic for P fimbriae only, but is also shared by some other adhesins that are not associated with pyelonephritis, especially S fimbriae. In the present study we have investigated whether human urine contains inhibitors of the binding of E. coli adhesins. Normal human urine was found to inhibit hemagglutination by S and type 1 fimbriae but not P fimbriae. The major inhibitor of S fimbriae in normal urine was identified as Tamm-Horsfall glycoprotein, and the interaction with S fimbriae is probably mediated by its sialyloligosaccharide chains. No significant variation was observed in the inhibitory effect of T-H glycoprotein preparations originating from different individuals. In contrast to S fimbriae, the major inhibitors of type 1 fimbriae in urine were identified as low-molecular-weight compounds. Gel filtration and ion-exchange chromatography and alpha-mannosidase treatment indicated that they were neutral alpha-mannosides, probably manno-oligosaccharides with three to five saccharides. Studies of urine samples collected from several individuals indicated the common occurrence of these inhibitory alpha-mannosides. Type 1 fimbriae bound to immobilized T-H glycoprotein, but, unlike S fimbriae, their binding was poorly inhibited by soluble T-H glycoprotein. Some urine samples were also found to contain low-molecular-weight inhibitors for the O75X adhesin of E. coli. These results emphasize that to function as a virulence factor in human urinary tract infections, an adhesin must evidently recognize such receptor structures at the infection sites that are not excreted in soluble form in urine. This prerequisite is filled by P fimbriae but not by type 1 or S fimbriae. 相似文献
13.
Clonal analysis of Escherichia coli O2:K1 isolated from diseased humans and animals. 总被引:10,自引:7,他引:10 下载免费PDF全文
M Achtman M Heuzenroeder B Kusecek H Ochman D Caugant R K Selander V Visanen-Rhen T K Korhonen S Stuart F Orskov et al. 《Infection and immunity》1986,51(1):268-276
Forty-six Escherichia coli isolates of serotype O2:K1 from human urinary tract infections, chicken sepsis, and bovine mastitis were obtained from laboratories in England, Denmark, Sweden, and Finland. The bacteria were compared for outer membrane protein (OMP) pattern, lipopolysaccharide pattern, electrophoretic mobilities of enzymes, and flagellar serotype and were tested for fimbriation, biotype, hydroxamate production, hemolysin production, antibiotic resistance, plasmid content, colicin production, and virulence in neonatal rats. Isolates from humans were assigned to two clonal groups; poultry isolates belonged to one of these clonal groups, whereas bovine isolates belonged to the other. Poultry and human isolates of the same clonal group could be distinguished only by their plasmid content. Strains within this group were heterogeneous with respect to biotype, fimbriation, virulence, and flagellar serotype. Human and bovine isolates of the second clonal group were distinguished by a minor change in OMP pattern and by their plasmid content. It is concluded that meaningful clonal groupings are best recognized by the combination of OMP and electrophoretic enzyme patterns. The O:K serotype can aid in the recognition of important subclones, whereas the other microbiological properties tested can vary widely within clonal groupings. Furthermore, we conclude that certain O:K serotypes can contain very different clonal groupings having little genetic relatedness. 相似文献
14.
Binding characteristics of Escherichia coli adhesins in human urinary bladder. 总被引:9,自引:11,他引:9 下载免费PDF全文
R Virkola B Westerlund H Holthfer J Parkkinen M Kekomki T K Korhonen 《Infection and immunity》1988,56(10):2615-2622
We studied domains in the human bladder that acted as receptors for Escherichia coli P, S, type 1, type 1C, and O75X fimbriae or adhesin and domains in the human kidneys that were receptors for E. coli type 1C fimbriae. Binding sites in frozen tissue sections were localized by direct staining with fluorochrome-labeled recombinant strains and by indirect immunofluorescence with the purified adhesins. In the bladder, the P and S fimbriae showed closely similar binding to the epithelial and muscular layers, and the S fimbriae also bound to the connective tissue elements. Type 1 fimbriae bound to vascular walls and to muscle cells, whereas the O75X adhesin bound avidly to connective tissue elements and to some extent to epithelial and muscle cells of the bladder. The type 1C fimbriae bound to distal tubules and collecting ducts of the kidney and to vascular endothelial cells in both the kidney and bladder. The binding of all adhesin types was inhibited by specific receptor analogs or Fab fragments. The results reveal a possible mechanism by which the type 1C fimbriae may help invasion of E. coli in the kidneys but do not support a pathogenetic role for type 1 fimbriae. Similar tissue specificity of P and S fimbriae in the human urinary tract indicates that the presence of binding sites on uroepithelia does not fully explain the virulence properties of P fimbriae in human urinary tract infections. 相似文献
15.
Lipopolysaccharide, capsule, and fimbriae as virulence factors among O1, O7, O16, O18, or O75 and K1, K5, or K100 Escherichia coli 总被引:10,自引:13,他引:10 下载免费PDF全文
B Kusecek H Wloch A Mercer V Vais?nen G Pluschke T Korhonen M Achtman 《Infection and immunity》1984,43(1):368-379
K1, K5, and K100 Escherichia coli isolates of the lipopolysaccharide antigen types O1, O7, O16, O18, or O75, which had formerly been assigned to clonal groupings were compared with K? E. coli isolates and with laboratory-derived mutants defective in capsule or lipopolysaccharide synthesis. The amount of K1 capsule, the length distribution of the lipopolysaccharide, and the expression of type I and P fimbriae were determined. The clonal groupings were uniform with regard to these properties within each group but different from each other. Many of the K? strains differed from the clonal representatives. The results are interpreted with regard to the different diseases caused by each of these bacterial groups. 相似文献
16.
Tatiana Aminoff Juhani Smolander Olli Korhonen Veikko Louhevaara 《European journal of applied physiology》1996,73(1-2):180-185
Ten young (aged 23–30 years) and nine older (aged 54–59 years) healthy men with similar estimated limb muscle volumes performed, in random order, three different types of ergometer exercise tests (one-arm cranking, two-arm cranking, and two-leg cycling) up to the maximal level. Values for work load (WL), peak oxygen consumption
, peak heart rate (HR), peak ventilation
, respiratory gas exchange ratio (R), recovery blood lactate concentration [La–], and rating of perceived exertion (RPE) were compared between the age-groups in the given exercise modes. No significant age-related differences in WL, peak
, peak HR, R, [La–], or RPE were found in one-arm or two-arm cranking. During one-arm cranking the mean peak
was 1.65 (SD 0.26)1 · min–1 among the young men and 1.63 (SD 0.10)1 · min–1 among the older men. Corresponding mean peak
during two-arm cranking was 2.19 (SD 0.32)1 · min-1 and 2.09 (SD 0.18)1 · min–1, respectively. During one-arm cranking peak
was higher (P < 0.05) among the older men compared to the young men. During two-leg cycling the young men showed higher values in WL (P < 0.001), peak
(P < 0.001), and peak HR (P < 0.001). The mean peak
was 3.54 (SD 0.24)1 · min–1 among the young men and 3.02 (SD 0.20)1 · min–1 among the older men. Corresponding mean peak HR was 182 (SD 5) beats · min–1 and 170 (SD 8) beats · min–1, respectively. During two-leg cycling, peak
, R, [La–], and RPE did not differ between the two age-groups. In summary, the older men with similar sizes of estimated arm and leg muscle volumes as the young men had a reduced physical work capacity in two-leg cycling. In one-arm or two-arm cranking, no significant difference in work capacity was found between the age-groups. These results indicate, that in healthy men, age, at least up to the 6th decade of life, is not necessarily associated with a decline in physical work capacity in exercises using relatively small muscle groups, in which the limiting factors are more peripheral than central. 相似文献
17.
Tarmi-Mattsson M Keskinen S Korhonen TT Lapinleimu H Tuominen J Niinikoski H Viikari J Ronnemaa T Välimäki I Simell O 《International journal of behavioral medicine》1997,4(4):310-322
Interventions aimed at decreased exposure of children to known atherosclerosis risk factors may have untoward behavioral side
effects. We examined how children’s behavior or parent’s perception of the behavior of the children at 3 years of age was
influenced by the intervention in a prospective randomized trial that began in infancy and effectively decreased scrum cholesterol
concentration. This Special Turku coronary Risk factor Intervention Project for babies (STRIP) began when the infant was 7
months old. Half of 1.062 children received individualized dietary counseling at 1-to 3-month intervals during the first 2
years of age and then half-yearly; the other half had an unrestricted diet. At 3 years of age a standardized questionnaire
of the child’s behavior was sent to 791 families (76% returned the questionnaire). At the onset of the trial the sociodemographic
data of the families and scrum lipid values of the intervention and control children were similar. Later, mean serum cholesterol
values of the intervention children remained constantly at a level 6% to 10% below the values of the control children. At
3 years of age the parental perceptions of the child’s behavior suggested minimal differences between the intervention and
control children. The intervention children were slightly less jealous and more active and creative, but showed slightly more
negative signs of behavior (bed-wetting, problems in falling asleep, fears) than the controls. We conclude that long-term,
individualized dietary and lifestyle intervention that begins in infancy slightly influences children’s behavior or parent’s
recognition of the behavior of the children at the age of 3 years.
This work was supported in part by grants from the Varsinais-Suomi Regional Fund of the Finnish Cultural Foundation, the Mannerheim
League for Child Welfare, the Academy of Finland, and the Alli Paasikivi Foundation. 相似文献
18.
Genetic relationships and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. 总被引:10,自引:10,他引:10 下载免费PDF全文
R K Selander T K Korhonen V Visnen-Rhen P H Williams P E Pattison D A Caugant 《Infection and immunity》1986,52(1):213-222
Genetic diversity and relationships among 63 isolates of Escherichia coli from infants in Finland with septicemia or meningitis were assessed by analyzing electrophoretic variation in 21 enzymes encoded by chromosomal genes. Thirty-nine multilocus genotypes (electrophoretic types) were distinguished, 23 of which formed a closely related, distinctive subset (group 1) of five or six clones represented by 40 (63%) of the isolates. The remaining isolates represented a second subset of 16 electrophoretic types (group 2) that were, on the average, rather more distantly related to one another. Although the number of electrophoretic types causing neonatal systemic disease is smaller than that occurring in healthy intestinal floras, the pathogenic electrophoretic types are only slightly less diverse genetically. Isolates of group 1 were characterized by relatively high incidences of hemolysin production and S, type 1, type 1C, and P fimbriae. However, because phenotypic characters, considered individually or in combination, did not adequately reflect the overall genetic relationships of isolates, it is recommended that the genetic structure of populations be defined on the basis of multilocus chromosomal genotypes. 相似文献
19.
Juhani Smolander Veikko Louhevaara Timo Tuomi Olli Korhonen Juhani Jaakkola 《International archives of occupational and environmental health》1984,54(3):261-270
Summary Six healthy men aged 25 to 37 walked on a treadmill at work levels of 21 and 41% of their
for 25 to 30 min wearing gas protective clothing (GPC) consisting of an impermeable suit with a self-contained breathing apparatus (total weight 25 kg) or shorts (control tests, CT) in a temperate environment (t
a 24.3°C ± 1.0°C, rh 30–50%). When the GPC was worn at 21 and 41%
, the most prominent increases, compared with the CT, were noted in the heart rate (
± SE, 120 ± 5 vs 76 ± 3 beats min–1 and 171 ± 5 vs 103 ± 3 beats min–1), mean skin temperature (36.1 ± 0.2 vs 31.3° C ± 0.1°C and 36.9 ± 0.3 vs 30.9°C ± 0.4°C) and sweat rate (473 ± 51 vs 70 ± 23 g m–2 h–1 and 766 ± 81 vs 135 ± 18 g m–2 h–1) indicating a high cardiovascular and thermoregulatory strain, which was not decreased by ventilating the suit with an air flow of 281 min–1 at 41%
. The ventilation, oxygen consumption and production of carbon dioxide increased in relation to the extra weight of the GPC, partly dependent on the dynamic work level. It was concluded that the increase in the physiological load caused by the GPC was so high that the work-rest regimens, workers' level of physical fitness, cardiovascular health and heat tolerance should be considered whenever gas protective clothing is used. 相似文献
20.
Marja Ala-Houhala Christer Holmberg Kai Rönnholm Aila Paganus Jarmo Laine Olli Koskimies 《Pediatric nephrology (Berlin, Germany)》1995,9(6):737-741
Alphacalcidol oral pulse therapy was given for secondary hyperparathyroidism to 22 children (mean age of 5.6 years) with renal insufficiency. At the beginning of the study, the glomerular filtration rate was <50% of normal, serum intact parathyroid hormone (PTH) was >100 ng/l and the serum phosphate and calcium concentrations were within the normal range. Alphacalcidol (0.5–3.0 g) was given orally thrice weekly in the evening and adjusted according to PTH, ionized calcium and phosphate concentrations. Serum PTH (mean ± SEM) decreased significantly from a pre-treatment level of 393±81 ng/l to 122±34 ng/l after 12 months, and stabilized at this level. Mean vitamin D metabolite concentrations were within the normal range. 1,25-Dihydroxyvitamin D did not increase during therapy, while PTH decreased. The estimated creatinine clearance remained almost unchanged (20±3 and 21±6 ml/min per 1.73 m2). Growth remained low normal (height standard deviation score –1.8±0.3 initially and –1.7±0.4 12 months later) and bone mineral density did not decrease. We concluded that feedback regulation of PTH with oral alphacalcidol pulse therapy is effective in the treatment of hyperparathyroidism in children with renal failure prior to dialysis. 相似文献