某电解铝厂职业病危害因素控制效果评价

)]监测点全部超标;工频电场检测点合格率为50.00%(3/6);工频磁场检测点合格率为96.00%(24/25).结论 该项目采取的职业病危害防护措施有效可行,基本达到职业病危害控制要求.职业病危害关键控制点是粉尘作业的供配料储运、炭阳极铸造操作位、电解岗位;高温作业的电解槽、炭阳极加热炉、铸造混合炉操作岗位.对其尚存氧化铝粉尘、WGBT(℃)等超限值现象,应限期进行整改.     

耐药葡萄球菌感染关节翻修假体带菌存留一例

关节置换术后感染的处理较困难,即便经历翻修,术后感染亦常复发,只能长期旷置、甚至采用髋关节融合来治疗.而甲氧西林耐药金黄色葡萄球菌(methicillin resistant staphylococcus aureus,MRSA)因其广泛和高度的耐药性,使感染后的治疗变得更为棘手.我们对1例人工股骨头置换术后MRSA感染行翻修术后多次复发的患者采用带菌存留假体、局部持续负压引流的方法治疗,随访30个月关节假体无明显松动,并获得较好的屈髋活动功能,报告如下.     

钽金属臼杯重建髋臼骨缺损的近期疗效

 目的 探讨应用钽金属臼杯对不同类型髋臼骨缺损进行重建的近期疗效。方法 回顾性分析2009年8月至2012年4月在全髋关节置换术中使用钽金属臼杯重建髋臼骨缺损的连续21例患者的病例资料,男6例,女15例;年龄24~78岁,平均(53.7±16.2)岁。初次全髋关节置换5例：Crowe Ⅳ型髋关节发育不良2例、髋关节感染后遗高位脱位畸形2例、类风湿关节炎1例;全髋关节翻修16例：全髋关节置换术后感染二期翻修2例、髋臼金属炎性假瘤3例、假体无菌性松动11例。骨缺损Paprosky分型Ⅰ型9例,Ⅱa型4例,Ⅱb型5例,Ⅱc型1例,Ⅲa型1例,Ⅲb型1例。观察术后髋臼假体周围透光线、假体位置、生存率、并发症及Harris髋关节评分。结果 全部病例随访6~40个月,平均20.1个月。末次随访时PaproskyⅠ型患者的Harris髋关节评分(87.2±7.4)分,高于术前(41.1±6.3)分;Ⅱ型(79.8±11.8)分,高于术前(38.4±12.5)分;Ⅲ型为79分,高于术前24分。随访期间钽金属臼杯固定良好,未出现松动及移位,假体生存率100%。按照Anderson骨长入影像学评价标准,髋臼杯均出现不同程度骨长入。1例于DeLee-Charnley髋臼3区出现透光线,4个月后消失;1例于髋臼1区出现假体周围间隙,随访6个月未进展。1例于术后4周出现后脱位。结论 应用钽金属臼杯重建PaproskyⅠ、Ⅱ型髋臼骨缺损,能实现早期与宿主骨整合,近期疗效好。     

18F-FECNT的生物分布特性及小动物PET显像研究

Objective 2β-carbomethoxy-3β-(4-corophenyl)-8-(2-18F-fluoroethyl) nortropane (18F-FECNT) is a recently developed dopamine transporter (DAT) imaging agent. The aim of this study was to evaluate its brain biedistribution and to assess its usefulness in quantitation of DAT density in normal and hemiparkinsonian rats. Methods Six groups of mice (5 mice each group) received 18F-FECNT were sacri-ficed at indicated time post injection. Different brain regions (cortex, hippocampus, striatum, cerebellum) were removed, weighed, and countered. DAT blocking effect was investigated in mice pretreated with 2β-Carbomethoxy-3β-(4-fluorpbenyl)tropane (β-CFT) at before 18F-FECNT injection. MicroPET scans were performed in beth normal and unilaterally 6-hydroxydopamine-lesioned rats. Results The brain uptake of 18F-FECNT was 2.22, 1.20, 1.02, 0.78, 0.71, and 0.67 percent injection dose (%ID) at 5, 15, 30, 60, 120, and 180 min post injection. Radioactivity concentration of the striatum, the target region, was the highest in the brain regions and decreased quickly from 5 to 60 min and reached to background at 120 min of post injection. The striatum/cerebellum ratio was 2.56, 3.47, 2.78, 1.63, 0.97, and 0.88 at 5, 15, 30, 60, 120, and 180 min, respectively, post injection. The selective striatum uptake of 18F-FECNT decreased dramatically to the background when the DAT was blocked with β-CFT. The striatum of normal rats in micro-PET exhibited symmetrical (left/right = 1.00±0.05) and the highest uptake of radioactivity (striatum/cere-helium =2.18±0. 16 at 5- 125 min, n =3). As for the hemiparkinsonian rats, nonsymmetrical [unlesioned striatum/cerebellum vs lesioned striatum/cerebellum = 2.01 ± 0.23 (n = 3) vs 1.04 ± 0. 05] and the high-est uptake of radioactivity were also noted. Conclusions The results suggest that 18F-FECNT rapidly pas-ses through blood-brain barrier and locates in stiatal region with high affinity and selectivity to DAT. It is a potential radiotracer to assess the in vivo DAT density in Parkinson's disease.     

18F-Fallypride的自动化合成与小动物PET显像

目的 研究高效、简单的自动化合成多巴胺D2受体显像剂(S)-(-)-N-(1-烯丙基吡咯烷-2-氨基甲基)-5-(3-18F)-2,3-二甲氧基苯甲酰胺(18F-Fallypride)的方法,并用小动物PET观察其在小鼠活体内的生物分布.方法 采用国产氟标记多功能模块控制整个过程,18F-在乙腈溶液中与前体(s)-(-)-N-(1-烯丙基吡咯烷-2-氨基甲基)-5-(3-磺酰基)-2,3-二甲氧基苯甲酰胺(OTSF)直接反应生成18F-Fallypride,混合物装柱,产品被C18柱吸附,用水冲洗柱,用少量乙醇淋出,加生理盐水稀释.ICR小鼠给药后经小动物PET活体显像.结果 18F-Fallypride放化产率为40.7%(已校正),合成时间为40 min,无需高效液相色谱(HPLC)法分离,放化纯＞95%.注射18F-Fallypride后ICR小鼠经小动物PET显像,脑内纹状体区域摄取最高,且双侧放射性浓聚对称,清除较慢.结论 18F-Fallypride自动化合成速度快,效率高.18F-Fallypride适于多巴胺D2受体显像.     

海洋源放线菌Streptomyces sp. SCSIO 01681次级代谢产物的研究

摘 要：目的 对从我国三亚鹿回头海水沉积物中分离得到的一株海洋放线菌SCSIO 01681进行鉴定并对其次级代谢产物进行研究。方法 通过16S rDNA序列分析并构建系统发育进化树来鉴定菌株,利用有机溶剂萃取、正相和反相硅胶层析等分离手段对海洋放线菌SCSIO 01681的发酵产物进行分离纯化,通过波谱数据分析及文献比较对化合物进行结构鉴定, 并进行了卤虫致死及抗菌活性评价。结果 该放线菌被鉴定为Streptomyces sp. SCSIO 01681,并从其发酵产物中分离得到3个化合物,其结构分别鉴定为苯乙酸（1）,亚油酸甘油酯（2）,邻苯二甲酸二（2-乙基己基）酯（3）;活性结果显示化合物1对嗜水气单胞菌、耐甲氧西林金黄色葡萄球菌、藤黄微球菌、粪链球菌具有抑制作用。结论 放线菌Streptomyces sp. SCSIO 01681能够产生3个医药、工业重要中间体,具有潜在的应用价值。     

18F-FECNT的生物分布特性及小动物PET显像研究

Objective 2β-carbomethoxy-3β-(4-corophenyl)-8-(2-18F-fluoroethyl) nortropane (18F-FECNT) is a recently developed dopamine transporter (DAT) imaging agent. The aim of this study was to evaluate its brain biedistribution and to assess its usefulness in quantitation of DAT density in normal and hemiparkinsonian rats. Methods Six groups of mice (5 mice each group) received 18F-FECNT were sacri-ficed at indicated time post injection. Different brain regions (cortex, hippocampus, striatum, cerebellum) were removed, weighed, and countered. DAT blocking effect was investigated in mice pretreated with 2β-Carbomethoxy-3β-(4-fluorpbenyl)tropane (β-CFT) at before 18F-FECNT injection. MicroPET scans were performed in beth normal and unilaterally 6-hydroxydopamine-lesioned rats. Results The brain uptake of 18F-FECNT was 2.22, 1.20, 1.02, 0.78, 0.71, and 0.67 percent injection dose (%ID) at 5, 15, 30, 60, 120, and 180 min post injection. Radioactivity concentration of the striatum, the target region, was the highest in the brain regions and decreased quickly from 5 to 60 min and reached to background at 120 min of post injection. The striatum/cerebellum ratio was 2.56, 3.47, 2.78, 1.63, 0.97, and 0.88 at 5, 15, 30, 60, 120, and 180 min, respectively, post injection. The selective striatum uptake of 18F-FECNT decreased dramatically to the background when the DAT was blocked with β-CFT. The striatum of normal rats in micro-PET exhibited symmetrical (left/right = 1.00±0.05) and the highest uptake of radioactivity (striatum/cere-helium =2.18±0. 16 at 5- 125 min, n =3). As for the hemiparkinsonian rats, nonsymmetrical [unlesioned striatum/cerebellum vs lesioned striatum/cerebellum = 2.01 ± 0.23 (n = 3) vs 1.04 ± 0. 05] and the high-est uptake of radioactivity were also noted. Conclusions The results suggest that 18F-FECNT rapidly pas-ses through blood-brain barrier and locates in stiatal region with high affinity and selectivity to DAT. It is a potential radiotracer to assess the in vivo DAT density in Parkinson's disease.     

启东市人民医院

<正>位于江苏省江海之滨的启东市人民医院始建于1949年,经过了40多年的艰辛创业历程,目前已是一所拥有编制床位380张,实际开放床位426张的县级综合性医院,同时,也是启东市医疗、教学和科研中心。全院占地面积36516m~2,总建筑面积35000m~2。现有在编职工583人,其中高级技术职称40人,中级技术职称136人。设有病区11个,医技科室5个,行政职能科室10个。曾多次荣获省、南通市授予的“文明医院”、“医德医风先进集体”等荣誉称号。1994年,被卫生部评为南通市第一批“二级甲等医院”、“爱婴医院”,并成为“南通医学院教学医院”。 院长(江苏省医院管理学会理事)沈超同志带领领导班子团结进取,开拓创新,使医院基本建设、业务技术、服务质量均有了长足的发展。特别是近几年来,医院班子思想解放、大胆探索,不断推进两个文明建设,医院固定资产从1992年的950万元增加到现在的2770万元;业务收入从1992年的1000万元增加到4000万元。经济效益的提高,给该院增加了活力,注入了勃勃生机,充分调动了职工的积极性和质量意识。     

68Ga-DOTA-NOC胰腺癌生长抑素受体靶向显像的实验研究

目的 研究68 Ga-1,4,7,10-四氮杂环十二烷-1,4,7,10-四乙酸-1-萘丙氨酸-奥曲肽(DOTA-NOC)在小鼠体内的生物分布,并进行荷胰腺癌小鼠的68Ga-DOTA-NOC SSTR靶向显像.方法 37 MBq 68GaCl2加入20μl羟乙基哌嗪乙硫磺酸(HEPES,0.1 mmol/L)溶液和10～50 μg DOTA-NOC,于95℃水浴加热15 min制备68Ga-DOTA-NOC.采用HPLC法分析68 Ga-DOTA-NOC的标记率及体外稳定性.予正常BALB/c小鼠尾静脉注射68Ga-DOTA-NOC 3.7 MBq,分别在5、15、30、60和120 min后解剖分离小鼠脏器,测放射性生物分布(％ID/g)情况.将人CFPAC-1胰腺癌细胞与68Ga-DOTANOC共同孵育后,测定放射性计数并计算摄取率.建立CFPAC-1荷瘤裸鼠模型,行microPET显像,勾画ROI,获得肿瘤及主要脏器TAC,计算T/NT(NT为对侧肌肉).用流式细胞术检测CFPAC-1细胞及瘤体SSTR表达水平,采用直线相关分析对SSTR表达率与T/NT行相关性研究.结果 68 Ga-DOTA-NOC标记率为(98.8±1.0)％,无需纯化.正常小鼠体内68Ga-DOTA-NOC肾摄取最多,5 min为(11.20±0.32) ％ID/g,随时间延长减少,60 min为(4.11±0.21)％ID/g,胰腺、肺、胃相对摄取较高,脑几乎无摄取.人CFPAC-1细胞可特异性摄取68Ga-DOTA-NOC.荷瘤鼠microPET显像示CFPAC-1肿瘤部位呈异常放射性摄取,T/NT在120 min达3.2±0.2.CFPAC-1细胞和肿瘤组织SSTR1、2、4、5表达量分别为(96.83±1.23)％、(99.73±0.98)％、(90.42±0.63)％和(92.96±0.71)％,SSTR2、5的表达水平与T/NT均呈正相关(r=0.71、0.80,均P＜0.05).结论 68 Ga-DOTA-NOC易标记,生物分布理想,可靶向结合SSTR阳性的CFPAC-1肿瘤组织.68Ga-DOTA-NOC是新型SSTR靶向显像剂,有利于胰腺肿瘤的早期诊断并为生长抑素介导靶向治疗提供依据.     

18F-FECNT的生物分布特性及小动物PET显像研究

Objective 2β-carbomethoxy-3β-(4-corophenyl)-8-(2-18F-fluoroethyl) nortropane (18F-FECNT) is a recently developed dopamine transporter (DAT) imaging agent. The aim of this study was to evaluate its brain biedistribution and to assess its usefulness in quantitation of DAT density in normal and hemiparkinsonian rats. Methods Six groups of mice (5 mice each group) received 18F-FECNT were sacri-ficed at indicated time post injection. Different brain regions (cortex, hippocampus, striatum, cerebellum) were removed, weighed, and countered. DAT blocking effect was investigated in mice pretreated with 2β-Carbomethoxy-3β-(4-fluorpbenyl)tropane (β-CFT) at before 18F-FECNT injection. MicroPET scans were performed in beth normal and unilaterally 6-hydroxydopamine-lesioned rats. Results The brain uptake of 18F-FECNT was 2.22, 1.20, 1.02, 0.78, 0.71, and 0.67 percent injection dose (%ID) at 5, 15, 30, 60, 120, and 180 min post injection. Radioactivity concentration of the striatum, the target region, was the highest in the brain regions and decreased quickly from 5 to 60 min and reached to background at 120 min of post injection. The striatum/cerebellum ratio was 2.56, 3.47, 2.78, 1.63, 0.97, and 0.88 at 5, 15, 30, 60, 120, and 180 min, respectively, post injection. The selective striatum uptake of 18F-FECNT decreased dramatically to the background when the DAT was blocked with β-CFT. The striatum of normal rats in micro-PET exhibited symmetrical (left/right = 1.00±0.05) and the highest uptake of radioactivity (striatum/cere-helium =2.18±0. 16 at 5- 125 min, n =3). As for the hemiparkinsonian rats, nonsymmetrical [unlesioned striatum/cerebellum vs lesioned striatum/cerebellum = 2.01 ± 0.23 (n = 3) vs 1.04 ± 0. 05] and the high-est uptake of radioactivity were also noted. Conclusions The results suggest that 18F-FECNT rapidly pas-ses through blood-brain barrier and locates in stiatal region with high affinity and selectivity to DAT. It is a potential radiotracer to assess the in vivo DAT density in Parkinson's disease.