肿瘤手术切缘的分子边界研究进展

目前,手术切除是治疗肿瘤的最主要手段之一,而术后局部复发是手术治疗失败和影响患者预后的重要原因。因此,手术切缘彻底与否成为影响肿瘤预后的主要因素,如何获得安全的手术切缘目前仍存在较大争议。传统方法判断手术切除是否干净主要基于对切缘组织的常规病理学(即细胞形态学     

吴茱萸穴位贴敷配合治疗小儿感染性发热的临床研究

目的:观察吴茱萸穴位贴敷配合治疗小儿感染性发热的临床研究。方法:治疗组采用吴茱萸穴贴敷治疗,每日晨起敷于患儿双涌泉、大椎穴;对照组口服布洛芬混悬液,必要时予补液等对症治疗;分别观察给药1h、2h、4h、6h内退热的情况。结果:治疗组患者体温均明显低于对照组(P<0.05),而在不良反应及依从性方面,对照组明显高于治疗组。结论:吴茱萸穴位贴敷双涌泉、大椎穴治疗小儿发热,具有退热效果、依从性好,不良反应较低等优势。     

火焰原子吸收光谱法测定饮料中镁含量的测量不确定度评定

目的评定火焰原子吸收光谱法测定饮料中镁含量的测量不确定度。方法根据测定方法建立数学模型,分析测量不确定度来源,计算各不确定度分量和扩展不确定度。结果当k=2（95%置信概率）时,饮料中镁的含量为（4.45±0.68）mg/L。结论测量不确定度的主要来源为样品定容体积引入的不确定度和曲线拟合引入的不确定度以及重复测定时产生的不确定度。     

FHIT基因过表达对人胃癌细胞系MKN-45增殖和粘附的影响

目的探讨FHIT基因过表达对人胃癌细胞系MKN-45增殖和粘附能力的影响。方法将载有人外源性FHIT基因的真核表达质粒pcDNA3.1-FHIT转染FHIT基因mRNA阴性表达人胃癌细胞系MKN-45,分别用MTT法、平板克隆形成实验、黏附实验检测转染前后细胞增殖活性、克隆形成能力及粘附能力的变化。结果FHIT基因的过表达可降低MKN-45细胞的增殖和克隆形成能力(P<0.05),但是对MKN-45细胞与内皮细胞的粘附能力无明显影响。结论FHIT基因过表达可抑制人胃癌细胞系MKN-45的增殖和克隆形成,对于MKN-45细胞与内皮细胞的粘附能力无明显影响。     

32P-磷酸铬-聚 L-乳酸缓释粒子的制备及其放射性释出和生物分布

Objective The aim of this study was to prepare the 32P-chromic phosphate-poly(L-lac-tide) (32P-CP-PLLA) particles with different ratio of the materials and further examine their performance in-dex in vivo and in vitro and their intracorporeal distribution. Methods The erosion, degrading rates, de-layed release velocity and radioactivity self-absorption coefficient (RSAC) of 32P-CP-PLLA particles made from different materials were investigated and compared. After the implantation of 32P-CP-PLLA particles and the injection of 32P-CP colloids in the muscular tissues, the weight loss rate and the radioactivity release rate (RRR) of the particles were calculated. The intracorporeal distribution, radioactive half-life and bio-logical effect of 32P in the targeting sites were further studied. Statistical analysis was performed with SPSS 12.0, and one-way analysis of variance and t-test were used. Results 32P-CP-PLLA particles were of green cylinder, with regular shape and radionuclide distribution. The RSAC of the particles was of little re-lation with molecular weight of PLLA and proportional to the ratio of PLLA to CP. The extracorporeal release rate increased with the reduction of molecular weight of PLLA and with the increase of the ratio of PLLA to CP. The RRR reached peak when PLLA was 3 times of CP. The 32P-CP, released with the degradation and corrosion of the particle distributed mainly in the surrounding muscles of the particle. And the peak of per-centage activity of injection dose per gram of tissue (% ID/g) in liver, spleen and bone were 1. 7887, 1. 6401 and 1. 9470 respectively, much lower than that in the 32P-CP group (4.7523, 3.9712 and 4.3174 ; all t > 2.7, all P < 0.05). The % ID/g in other organs was much less. The radioactivity effective half-life in the targeting sites increased to about 13 d. There was widespread necrosis around the particles with no ex-istence of normal tissues among them. And no abnormality in spleen and liver was found. Conclusion As a better dosage form of pure β-particle emitter, 32P-CP-PLLA, which can increase the targeting radioactive dosage and effective half-life in the implanting sites, can be served as an potential implanting agent for onco-therapy with a better perspective.     

32P-磷酸铬-聚 L-乳酸缓释粒子的制备及其放射性释出和生物分布

Objective The aim of this study was to prepare the 32P-chromic phosphate-poly(L-lac-tide) (32P-CP-PLLA) particles with different ratio of the materials and further examine their performance in-dex in vivo and in vitro and their intracorporeal distribution. Methods The erosion, degrading rates, de-layed release velocity and radioactivity self-absorption coefficient (RSAC) of 32P-CP-PLLA particles made from different materials were investigated and compared. After the implantation of 32P-CP-PLLA particles and the injection of 32P-CP colloids in the muscular tissues, the weight loss rate and the radioactivity release rate (RRR) of the particles were calculated. The intracorporeal distribution, radioactive half-life and bio-logical effect of 32P in the targeting sites were further studied. Statistical analysis was performed with SPSS 12.0, and one-way analysis of variance and t-test were used. Results 32P-CP-PLLA particles were of green cylinder, with regular shape and radionuclide distribution. The RSAC of the particles was of little re-lation with molecular weight of PLLA and proportional to the ratio of PLLA to CP. The extracorporeal release rate increased with the reduction of molecular weight of PLLA and with the increase of the ratio of PLLA to CP. The RRR reached peak when PLLA was 3 times of CP. The 32P-CP, released with the degradation and corrosion of the particle distributed mainly in the surrounding muscles of the particle. And the peak of per-centage activity of injection dose per gram of tissue (% ID/g) in liver, spleen and bone were 1. 7887, 1. 6401 and 1. 9470 respectively, much lower than that in the 32P-CP group (4.7523, 3.9712 and 4.3174 ; all t > 2.7, all P < 0.05). The % ID/g in other organs was much less. The radioactivity effective half-life in the targeting sites increased to about 13 d. There was widespread necrosis around the particles with no ex-istence of normal tissues among them. And no abnormality in spleen and liver was found. Conclusion As a better dosage form of pure β-particle emitter, 32P-CP-PLLA, which can increase the targeting radioactive dosage and effective half-life in the implanting sites, can be served as an potential implanting agent for onco-therapy with a better perspective.     

内毒素休克及其肾上腺皮质损伤机制的研究进展

内毒素是存在于革兰阴性菌细胞壁外膜中的一种特征性成分，由于其化学成分含有多糖和类脂，故而又称脂多糖（LPS），内毒素在体内可激活单核／巨噬细胞、内皮细胞等合成和释放各种细胞因子和炎症介质，导致血管通透性增加、体液渗出、淋巴细胞移行到炎症部位，这本是机体的一种防御性反应，有利于清除病原菌和机体的恢复，但若反应过度，则可引起全身难以控制的“瀑布式炎症级联反应”或伴免疫功能的严重抑制，即全身炎症反应综合征（systemic inflammatory response syndwme，SIRS）或代偿性抗炎反应综合征（compensatory anti—inflammatory reacfion syndrome，CARS），     

增液承气汤治疗粘连性肠梗阻78例

增液承气汤治疗粘连性肠梗阻７８例甘肃省中医院（７３００５０）黄培林甘肃省肿瘤医院（７３００５０）陈汝贞临床资料本组７８例，其中男性５７例，女性２１例；年龄在３～６１岁；既往有手术史者７１例：曾行阑尾切除术者１６例、肠梗阻手术１７例、胃穿孔修补术９例、...     

结肠癌细胞上调脐静脉内皮细胞EMMPRIN的表达

目的：探讨结肠癌细胞对脐静脉内皮细胞中细胞外基质金属蛋白酶诱导因子（EMMPRIN）表达的影响,以及EMM-PRIN与肿瘤新生血管生成的关系.方法：建立结肠癌LoVo细胞与人脐静脉内皮细胞（HUVEC）共培养模型,并设HUVEC单独培养对照组,培养36 h后进行形态学观察,并应用免疫细胞化学方法检测内皮细胞EMMPRIN的表达.结果：共培养组HUVEC形成大量管样结构,且EMMPRIN的表达明显强于HUVEC单独培养对照组强.结论：结肠癌细胞上调HUVEC EMMPRIN的表达,并诱导HUVEC管样结构的的形成,EMMPRIN的表达可能与肿瘤新生血管生成具有一定的相关性.     

脂肪酸合酶抑制剂对大肠癌细胞NF-κB活性的抑制作用

目的　探讨脂肪酸合酶抑制剂对大肠癌细胞NF κB活性的抑制作用。方法　用脂肪酸合酶抑制剂(FASI) 浅蓝菌素处理大肠癌LoVo细胞株 ,用凝胶电泳迁移率法 (EMSA)检测NF κB活性。结果　实验分别用不同浓度浅蓝菌素 (10 -9～ 10 -5M )处理LoVo细胞 12h后 ,可抑制NF κB活化 ,其抑制程度呈剂量依赖性递减 ,与对照组相比较浅蓝菌素 (10 -9～ 10 -5M )处理LoVo细胞 12h后NF κB条带扫描像素积分比分别为 0 .787,0 .45 1,0 .379,0 .338,0 .32 2。结论　浅蓝菌素处理人大肠癌LoVo细胞后 ,NF κB活化程度随浅蓝菌素的剂量递增而递减。提示浅蓝菌素对LoVo细胞NF κB活性的具有抑制作用。