应用X线计算机图像分析及生物力学方法评估富血小板血浆促进兔尺骨骨折愈合的研究

目的 应用CR钼靶X线仪计算机图像分析及生物力学方法评估富血小板血浆在促进兔尺骨骨折愈合的效果.方法 将实验动物随机分为4组(空白组,血小板组,植骨+血小板组,正常对照组)前3组建立尺骨骨折动物模型,并应用指骨钢板内固定.将制备的富血小板血浆回注入骨折端.术后2、4、8周对3组标本骨折处骨痂进行大体观察,骨痂直径测量,并行CR钼靶X线照射,图像分析仪计算骨痂灰度密度积分.对术后8周的标本及正常尺骨行生物力学测试.结果 术后2周,植骨+血小板组和血小板组骨折端周围,以纤维组织连接为主,仅有稀少的骨痂,空白组仅有少量纤维组织连接;骨痂直径和骨痂灰度密度积分顺序是植骨+血小板组,血小板组,空白组;术后4周,植骨+血小板组和血小板组有大量骨痂形成,部分骨痂骨化,而空白组以纤维性骨痂为主.骨痂直径和骨痂灰度密度积分顺序与术后2周相同;术后8周植骨+血小板组和血小板组骨折端周围已经形成骨性连接并塑形,空白组骨痂大部分骨化,有一定弹性.生物力学各项数据测试结果显示植骨+血小板组与正常对照组最接近,其次是血小板组,这3组均与空白组有显著性差异.结论 骨折局部应用富血小板血浆对骨折愈合具有一定的促进作用.     

骨折愈合机制的研究进展

骨折愈合是一个复杂的生理过程，在愈合过程中涉及多种细胞和细胞因子的协同作用。最近研究发现Hedgehog（Hh）家族作为一种分泌蛋白和信号分子在脊椎动物发育和骨折愈合过程中起一定的作用。除骨组织本身外，神经组织及其所含NP也参与骨折愈合。骨科医师很有必要了解骨折愈合中发生于细胞和分子水平的现象，探讨骨折不愈合及延迟愈合的机制，以便能采取针对性的措施以促进骨折愈合。     

The effect of PDGFmRNA and IGFmRNA in the platelet concentrated liquid on ulna fracture healing in rabbit

Objective By hybridization in situ and biomechanical approach of platelet-derived growth fator mRNA(PDGFmRNA) and insulin-like growth factor mRNA(IGFmRNA), we discussed the influence of the platelet conentrated liquid on the healing of rabbit ulna fracture.Method We selected 24 New Zealand rabbits,divided them into 4 groups randomly (blank group,serum-control group,group with platelet concentrated liquid and group with bone graft and platelet concentrated liquid), and then made the fracture model on the middle of ulna which was fixed by finger armor plate.Before the operation, we drew out 6 ml blood from femoral artery, performed anti-coagulation with the Sodium Citrate and centrifugated by low and the followed high speed. We purified the white blood plate and injected it into the fracture position. The rabbits were killed at 1st,2nd,5th and 6th week.Qualitative analysis by hybridization in situ of PDGFmRNA and IGFmRNA and biomechanical measurement on the yth week sample were made. Result Bone callus could be seen on the radius spectimen in various degrees when the rabbits were killed at 1st. 2nd, 4th and 6th week, particularly in the last week. The average maximum destructive load on the fracture tip is higher to the control, and there is significant difference (P&;lt;0.01). Conclusion The local application of platelet concentration on the fracture tip can accelerate its healing.     

儿童噬血细胞性淋巴组织细胞增生症中枢神经系统受累的影像特点及临床相关性

目的：研究儿童噬血细胞性淋巴组织细胞增生症（hemophagocytic lymphohistiocytosis,HLH）中枢神经系统（central ner－vous system,CNS）受累的影像表现及其与临床的相关性。方法：回顾性分析2012年1月至2018年11月于重庆医科大学附属儿童医院确诊的90例HLH中枢神经系统受累（CNS-HLH）患儿的影像和临床资料,按其影像表现分为3组,即阴性组（第1组）、蛛网膜下腔和（或）脑室扩大组（第2组）、脑实质病变组（第3组）。利用趋势性卡方检验分析各组间脑脊液检查阳性率、脑电图阳性率和临床表现阳性率的变化趋势,非参数检验（Kruskal-Wallis秩和检验）分析各组患儿独立风险因素的差异性。结果：第1组30例（33.3%）,第2组34例（37.8%）,第3组26例（28.9%）;CNS-HLH头颅影像表现多样,其中,第3组患儿影像学表现可分为小病灶（46.2%）、大片或弥漫性病灶（34.6%）、出血性病灶（19.2%）３型。3组患儿的脑脊液检查阳性率、脑电图阳性率、临床表现阳性率与组序号呈线性正相关（均P＜0.05）。3组患儿的脑脊液（cerebrospinal fluid,CSF）微量蛋白含量及细胞数、血小板计数（platelet count,PLT）具有差异性（均P＜0.05）,组间两两比较,P值经Bonferroni校正显示,第1组与第2组之间仅有CSF细胞数有统计学差异（均P＜0.05）,第1组与第3组及第2组与第3组间的CSF蛋白含量、细胞数和PLT均有统计学差异（均P＜0.05）。结论：HLH脑部影像具有一定的规律性,可反映疾病的严重程度和预后,为临床调整治疗方案提供影像学依据。     

用BFA－100对人体组成的研究

人体是由脂肪和瘦体组织组成的，一般成人脂肪占体重的２５％，是人体能源库。营养不良时，这些组成成分即发生变化。本文所用ＢＦＡ－１００是由北京军事医学研究所王育才等人研制的人体脂肪分析仪。该分析仪是利用人体中电导和体内含水量、离子浓度有关的原理进行设计。...     

米粉ORS治疗婴幼儿轮状病毒性肠炎合并脱水

我院收治60例婴幼儿腹泻,经实验室病原学检测(ELISA聚丙烯酰胺凝胶电泳、电镜)及临床资料均符合轮状病毒性肠炎的诊断。对伴有轻、中度脱水病例应用米粉ORS与WHO推荐的ORS(下称复方ORS),对照观察疗效。资料与方法:以随机抽样的方法,将60例分为米粉ORS治疗组(28例)和原方ORS治     

积雪草总苷对高脂血症小鼠的干预作用

目的 观察积雪草总苷对高脂乳剂诱导的高脂血症小鼠的血脂及肝脏调节作用.方法 以高脂肪乳剂连续灌胃制作小鼠高脂血症模型,给予不同剂量的积雪草总苷予以干预,以复方蛋氨酸胆碱为阳性对照组,1周后检测小鼠血清以及肝组织匀浆TC、TG、HDL-C、ALT、AST含量以及小鼠肝脏组织病理变化,以评价积雪草总苷干预效果.结果 积雪草...     

北京地区护士生活质量调查分析

目的调查北京地区护士生活质量,了解护士生活质量现状,为改善护士生活状况提供依据。方法采用中文版SF-36生活质量问卷调查表(Medical Outcomes Study,Short Form 36,SF-36),于2011年5月至2011年9月对北京地区12家医院,共365名临床一线在职护士进行调查,并与北京地区两家体检机构所收集的无慢性疾病的普通健康人共184例作对照分析,用SPSS 18.0系统软件进行统计处理。结果与健康组相比,护士组在PF(身体功能)方面高于健康组(P<0.05),而在RP(生理职能)、BP(躯体疼痛)、SF(社会功能)、RE(情感职能)、MH(精神健康)五个个方面明显低于健康对照组(P<0.05)。结论护理人员应该充分调动工作积极性,发挥优质护理优势,体现高水平护理的应用价值,提高自身成就感,为提高自身生活质量水平做出最大努力。     

中医康复护理改善肛肠科术后疼痛水肿的疗效分析

目的观察改善肛肠科术后疼痛水肿中医康复护理干预的效果。方法根据就诊顺序单双数分组法选取2016年12月—2018年6月该院就诊的56例改善肛肠科术后疼痛水肿患者进行研究,单数为实验组,双数为对照组,每组人数均为28例。给予对照组患者常规护理干预,在常规护理的基础上给予实验组患者中医康复护理干预。康复护理完成后,对患者采用VAS评分法对患者的术后疼痛感进行评估,并对两组患者术后的创面水肿状况进行比较,分析两种方案的有效性。结果实验完成后,两组患者的疼痛感和创面水肿状况均有所改善,但实验组改善状况更为明显,组间差异有统计学意义(P0.05)。结论在改善肛肠科术后疼痛水肿患者的护理中,采取中医康复护理干预效果更佳,能够对患者的疼痛感有所改善,有助于提高患者的护理满意度,具有较高的临床运用价值。     

促红细胞生成素促进坐骨神经再生的实验研究

Objective To investigate the effect of recombinant human erythropoietin(rh-EPO) on the nerve regeneration of adult rats sciatic nerves. Methods Tirty-six healthy male Wistar rats were involved and left sciatic nerve repaired model was used.The experimental rats were divided randomly into two groups:the EPO group and the control group,18 rats in each group.rh-EPO 3 000 U/kg was injected daily into the abdominal in EPO group,and normal saline was injected into the abdominal every day after operation in control group.On 4 and 8 weeks after operation,these items were determined,the sciatic function index (SFI),biomechanics examination,histological observation,electrophysiological examination,myelinated fibers density and sectional area measurement.Results On weeks 4 after operation,the SFI of EPO group and control group were-65.26 ± 3.42 and-70.83 ± 4.12,respectively,the maximum tensile resistance were (3.86 ± 0.29)N/mm2 and (3.38 ± 0.21 )N/mm2,the delayed ratio of latency of motor nerve were 2.34 ± 0.23 and 2.78 ± 0.29,and the recovery ratio of wave amplitude were 0.23 ± 0.05 and 0.14 ± 0.03 respectively.On eight weeks after operation,the SFI of EPO group and control group were-51.34 ± 2.98 and-57.23 ± 4.86,respcetively,the maximum tensile resistance were (4.67 ± 0.36) N/mm2 and (4.13 ± 0.32) N/mm2,the delayed ratio of latency of motor nerve were 1.32 ± 0.15 and 1.62 ± 0.21,the recovery ratio of wave amplitude were 0.41 ± 0.09 and 0.26 ± 0.07,the nerve fibers cross ratio were 0.57 ± 0.05 and 0.38 ± 0.03,and the recovery ratio of sectional area of myelinated fibers were 0.81 ± 0.06 and 0.58 ± 0.03,respectively.Those items in EPO group were significantly superior to those in the control group (P ＜ 0.05 ＝.Conclusion rh-EPO can promote the injured nerve regeneration and improve the recovery of their function.