不同持续时间的周期性压力对构建组织工程软骨的影响

Objective To determine the best duration for exerting the cyclic pressure under which the tissue-engineered cartilage is constructed. Methods Free chondrocytes isolated from rabbit articular cartilage were seeded into polylactic acid-co-glycolic acid(PLGA) scaffolds after expansion in vitro, and ran-domized into 4 groups. In Groups 1 to 3, chondrocytes were cultured under daily cyclic pressure (0 ～ 200 kPa, 0.1Hz) for 4 hours, 8 hours, 12 hours respectively; Group 4 was a control in which no pressure was exerted. In each group, after 2 weeks of culture, the tissue engineered cartilages were observed in vitro and assessed by his-tological staining of liE. Next, the content of DNA and the secretion of type Ⅱ collagen and GAG in cartilages were detected quantitatively. Results Under the daily cyclic pressure (0 ～200 kPa, 0.1 Hz), the scaf-fold-chondrocytes complex in the group of 8 hours got the largest volume, smooth, lucidus, and elastic surface, the most queuing chondrocytes, and the highest content of type Ⅱ collagen and GAG (P < 0.01). Conclusions Since chondrecytes are baro-senstive, the metabolism of chondrocytes can be affected by the time of cyclic pressure. Under the effect of 0 ～200 kPa, 0.1Hz, the daily cyclic pressure of 8 hours may be optimal for chondrocytes to multiply and synthesize extracellular matrixes such as type Ⅱ collagen and GAG.     

痹证治“痰”

<正> 痹证一病,乃风寒湿热诸邪客于经络,气血运行不畅所致的肌肉、关节、筋脉酸重疼痛,甚或出现关节肿大畸形,或伴见皮下结节。关于痹证,从古至今论述颇多,总以祛风蠲痹,除湿通络为其治疗大法。然而,在临床实践中体会到痹证日久,正虚邪恋,痰浊内生,或邪阻经络,津聚成痰,则见关节肿大畸形,或见皮下结节的病例,往往单用祛风湿之品,收效罔然,而参入化痰通络之品,则可提高疗效,现介绍如下,并浅谈体会。     

婴儿腮腺血管瘤54例临床病理分析

专题报道婴儿腮腺血管瘤者较少见,本组54例婴儿腮腺血管瘤,进行临床病理分析,其中毛细血管瘤37例,海绵状血管瘤8例,混合型血管瘤8例,淋巴管血管瘤1例。按血管内皮细胞增生程度,组织学分为Ⅲ级:I级13例,Ⅱ级21例,Ⅲ级20例。对本瘤的治疗方案、血管瘤自行消退机制进行了探讨,并提出本瘤以手术治疗为最佳方案以及并发症的予防。     

判断宫颈成熟度与促进宫颈成熟的方法

随着对宫颈的组织学、生化学和内分泌调节研究的进展,宫颈在分娩中的作用越来越引起产科学者的关注。顺利的自产和引产是子宫收缩和宫颈扩张相互协调作用的结果。但宫颈的扩张已不再被认为是被动的由于子宫体肌肉收缩牵引的结果。相反,宫颈在妊娠期并不是静止的,而是在多种因子调节下,经历着生物合成和降解同时进行的周期变化。这种改变到妊娠后期特别明显,称之为宫颈成熟,包括颈管变短、消失、软化和扩张等过程。临床实践证实宫颈的成熟异常对母儿会构成威胁。例如宫颈的过早成熟能引起早产,而足月妊娠宫     

CA125测定对某些妇科肿瘤的诊断意义

本文对４０名正常妇女和２０２名妇科肿瘤、宫内膜异位症的患者进行了血清ＣＡ１２５的测定。结果表明９８％正常妇女血清ＣＡ１２５水平＜５０ｕ／ｍｌ，行经期水平高于滤泡期、黄体期和绝经期水平。五组妇科疾病患者血清ＣＡ１２５水平也进行了测定，并观察了２１名卵巢癌患者ＣＡ１２５水平的变化情况。卵巢癌患者ＣＡ１２５水平最高，它与其它疾病组及正常对照组有明显的差异。血清ＣＡ１２５的测定在妇科临床上是有价值的。     

脊柱后凸和后侧凸畸形与肺心病

年龄较轻是脊柱后凸和后侧凸畸形所致肺心病的特点。患肺功能障碍和动脉血气异常程度与脊柱畸形类型和脊柱畸形角度显相关。即脊柱后凸或后侧凸≥９０°，动脉血氧分压（ＰａＯ２）和用力肺活量（ＦＶＣ）的降低较脊柱畸形〈９０°显（Ｐ〈０．０５），后侧凸畸形ＰａＯ２与ＦＶＣ显低于后凸畸形（Ｐ〈０．０５）。脊柱后凸或后侧凸患由于心脏、血管在胸腔位置的变化，使肺心病的Ｘ线片和心电图诊断率明显低于一般     

美多心安致休克二例

因使用美多心安引起休克二例,予以报道。例1男,67岁。因慢性支气管炎,阻塞性肺气肿,右心功能不全Ⅱ级住院。既往有冠心病史。EKG示快速型房颤,经消炎、利尿和地高辛治疗,症状好转。但心率快,加用美多心安50mg,每日二次。二天后(共用药200mg),突然神志朦胧,面色苍白,四肢厥冷,血压测