婴幼儿血管瘤的局部药物治疗进展

目的 婴幼儿血管瘤是婴幼儿最常见的良性软组织肿瘤。普萘洛尔治疗婴幼儿血管瘤的安全性和有效性均优于传统的糖皮质激素,但口服普萘洛尔可导致系统性并发症。局部应用β受体阻滞剂成为婴幼儿血管瘤治疗研究的新热点,如局部外用0.5%噻吗洛尔滴眼液、0.1%噻吗洛尔凝胶、普萘洛尔纳米水溶胶等。另外,局部外用5%咪喹莫特软膏、局部注射药物以及联合不同波长激光等均可作为婴幼儿血管瘤的治疗选择。本文简要综述婴幼儿血管瘤局部药物治疗方法。     

西罗莫司和饥饿处理对人A431细胞自噬水平的影响

目的 探讨经典自噬诱导剂西罗莫司和饥饿处理对人皮肤鳞状细胞癌细胞系A431自噬水平的影响。方法 分别将A431细胞和人宫颈癌细胞系HeLa分为对照组（单纯DMEM培养基处理）、二甲基亚砜（DMSO）组、20 nmol/L西罗莫司组、80 nmol/L西罗莫司组、平衡盐溶液（EBSS）组。作用4 h后,Western印迹检测A431细胞和HeLa细胞自噬微管相关蛋白轻链3A/3B（LC3A/B）、重组人γ?氨基丁酸受体相关蛋白（GABARAP）的表达水平。吖啶橙染色分析细胞自噬体表达水平。结果 Western印迹检测显示,对照组与DMSO组A431细胞中LC3A/B?Ⅱ与LC3 A/B?Ⅰ比值差异无统计学意义（P > 0.05）;20 nmol/L西罗莫司组、80 nmol/L西罗莫司组、EBSS组LC3 A/B?Ⅱ与LC3 A/B?Ⅰ比值较对照组均明显升高,差异均有统计学意义（P < 0.05）。HeLa细胞Western印迹检测结果与A431细胞类似。双变量相关性分析显示,HeLa细胞中LC3A/B?Ⅰ与GABARAP表达呈正相关（r = 0.869,95% CI：0.807 ~ 0.999,P = 0.051）,LC3A/B?Ⅱ与GABARAP表达呈负相关（r = -0.742,95% CI：-0.982 ~ 0.406,P = 0.042）;A431细胞中LC3A/B?Ⅰ与GABARAP表达亦呈正相关（r = 0.837,95% CI：-0.173 ~ 0.989,P = 0.037）,LC3A/B?Ⅱ与GABARAP表达呈负相关（r = -0.684,95% CI：-0.977 ~ 0.500,P = 0.047）。吖啶橙染色显示,A431细胞自噬阳性细胞比例在西罗莫司组（23.750% ± 0.260%）与EBSS组（32.450% ± 0.488%）同样高于对照组（15.987% ± 0.242%,均P < 0.05）;HeLa细胞自噬阳性细胞比例在西罗莫司组（33.307% ± 0.715%）和EBSS组（66.097% ± 1.141%）高于对照组（14.117% ± 0.295%,均P < 0.05）。结论 经典自噬诱导剂西罗莫司和饥饿能够上调A431细胞的自噬水平, GABARAP蛋白可能与LC3A/B高度相关。     

广东省免费孕前优生健康检查地中海贫血基因检测结果分析

目的:分析近两年广东省免费孕前优生健康检查地中海贫血基因检测结果,为地中海贫血防控工作提供依据。方法:以广东省各县区参加免费孕前优生健康检查的新婚夫妇为研究对象,MCV≤82fl或(且)MCH≤27pg作为地中海贫血筛查阳性指标,夫妇双方筛查均为阳性时,需同时进行α及β地中海贫血基因检测。结果:16240例筛查阳性者中发现基因携带者12995例(80.0%)准确率为80.0%,部分县区90%。发现3382对夫妇有妊娠重型地中海贫血胎儿的可能,产前诊断的需求率为41.65%。结论:MCV联合MCH作为筛查地中海贫血阳性指标可靠,操作简单,便于在基层应用,但需加强实验室质量控制。     

东亚人皮肤老化的特点

皮肤老化包括外源性老化和内源性老化.内源性老化又称自然老化,与年龄增长相关;而外源性老化主要与紫外线辐射有关,通常称其为光老化.     

东亚人皮肤老化的特点

皮肤老化包括外源性老化和内源性老化.内源性老化又称自然老化,与年龄增长相关;而外源性老化主要与紫外线辐射有关,通常称其为光老化.     

变应性亚败血症1例

变应性亚败血症１例张国毅①曾学思①顾恒①变应性亚败血症，是一种原因不明，临床上皮损多样、易变，伴关节痛、不规则发热，血象高，对抗生素治疗无效的少见皮肤病。多发生于儿童，成人少见。国内已报道４０余例。我们所见患者为一青年男性（２９岁），因躯干、四肢反复...     

光敏性银屑病—临床和光敏试验结果

作者对35例夏季日晒可使病情恶化的光敏性银屑病患者进行了研究.究内容包括:1.病史,详细询问他们的自然病史、可能存在的光敏性疾病和药物所致光敏等情况,2.实验室检查,进行了ANA、抗Ro抗体、尿卟啉、红细胞原卟啉、血常规、肝功能和蛋白电泳等检查,3.光激发试验,用高压氙灯力WG 295滤光片作为UVB光源,以其3～5倍的MED作为UVB激发试验剂量,用高压氙灯加窗玻璃滤过以获得UVA,在测得其红斑阈值后,以不超过阈值10倍的剂量作为激发剂量,4.光斑贴试验.     

寻常性银屑病患者外周血单一核细胞IFN-γ受体和TNF-α受体mRNA表达的研究

Objective To investigate the mRNA expressions of IFN-gamma receptor and TNF-alpha receptor in peripheral blood mononuclear cells (PBMCs) of patients with psoriasis vulgaris and their role in the pathogenesis of psoriasis. Methods Fifty patients with psoriasis vulgaris (37 cases of active psoriasis and 13 cases of stable psoriasis) and 24 healthy human controls were included in this study. PBMCs were isolated from blood samples obtained from all patients and controls. The mRNA expressions of IFN-gamma receptor and TNF-aipha receptor in PBMCs were detected by RT-PCR. The disease severity in patients was evaluated by psoriasis area and severity index (PASI). Results The mRNA expressions of IFN-gamma receptor and TNF-alpha receptor were observed in the PBMCs of all subjects. The mRNA expression levels of IFN-gamma receptor were 0.72 ± 0.17 in healthy controls, 1.11 ± 0.55 in all patients with psoriasis, 1.13 ±0.57 in patients with active psoriasis and 1.03 ± 0.52 in patients with stable psoriasis, respectively. A signifi-cant increase was observed in the expression levels of IFN-gamma receptor mRNA in all psoriatic patients and in patients with active psoriasis compared with those in healthy controls (both P < 0.05), but there was no significant difference between the healthy controls and patients with stable psoriasis (P ＞ 0.05). The expres-sion levels of TNF-alpha receptor mRNA were 2.05 ± 1.34 in healthy controls, 2.70 ± 3.80 in all psoriatic patients, 2.90 ± 4.40 in patients with active psoriasis, 2.14 ± 1.05 in patients with stable psoriasis, respectively;there was no significant difference between psoriatic patients and healthy controls (P ＞ 0.05). However, no correlation was found between the mRNA expression of IFN-gamma receptor, that of TNF-alpha receptor,and disease severity in psoriatic patients. Conclusions The mRNA expression of IFN-gamma receptor in PBMCs is up-regulated in patients with psoriasis vulgaris, which is unrelated to the activity of psoriasis.     

寻常性银屑病患者外周血单一核细胞IFN-γ受体和TNF-α受体mRNA表达的研究

Objective To investigate the mRNA expressions of IFN-gamma receptor and TNF-alpha receptor in peripheral blood mononuclear cells (PBMCs) of patients with psoriasis vulgaris and their role in the pathogenesis of psoriasis. Methods Fifty patients with psoriasis vulgaris (37 cases of active psoriasis and 13 cases of stable psoriasis) and 24 healthy human controls were included in this study. PBMCs were isolated from blood samples obtained from all patients and controls. The mRNA expressions of IFN-gamma receptor and TNF-aipha receptor in PBMCs were detected by RT-PCR. The disease severity in patients was evaluated by psoriasis area and severity index (PASI). Results The mRNA expressions of IFN-gamma receptor and TNF-alpha receptor were observed in the PBMCs of all subjects. The mRNA expression levels of IFN-gamma receptor were 0.72 ± 0.17 in healthy controls, 1.11 ± 0.55 in all patients with psoriasis, 1.13 ±0.57 in patients with active psoriasis and 1.03 ± 0.52 in patients with stable psoriasis, respectively. A signifi-cant increase was observed in the expression levels of IFN-gamma receptor mRNA in all psoriatic patients and in patients with active psoriasis compared with those in healthy controls (both P < 0.05), but there was no significant difference between the healthy controls and patients with stable psoriasis (P ＞ 0.05). The expres-sion levels of TNF-alpha receptor mRNA were 2.05 ± 1.34 in healthy controls, 2.70 ± 3.80 in all psoriatic patients, 2.90 ± 4.40 in patients with active psoriasis, 2.14 ± 1.05 in patients with stable psoriasis, respectively;there was no significant difference between psoriatic patients and healthy controls (P ＞ 0.05). However, no correlation was found between the mRNA expression of IFN-gamma receptor, that of TNF-alpha receptor,and disease severity in psoriatic patients. Conclusions The mRNA expression of IFN-gamma receptor in PBMCs is up-regulated in patients with psoriasis vulgaris, which is unrelated to the activity of psoriasis.     

寻常性银屑病患者外周血单一核细胞IFN-γ受体和TNF-α受体mRNA表达的研究

Objective To investigate the mRNA expressions of IFN-gamma receptor and TNF-alpha receptor in peripheral blood mononuclear cells (PBMCs) of patients with psoriasis vulgaris and their role in the pathogenesis of psoriasis. Methods Fifty patients with psoriasis vulgaris (37 cases of active psoriasis and 13 cases of stable psoriasis) and 24 healthy human controls were included in this study. PBMCs were isolated from blood samples obtained from all patients and controls. The mRNA expressions of IFN-gamma receptor and TNF-aipha receptor in PBMCs were detected by RT-PCR. The disease severity in patients was evaluated by psoriasis area and severity index (PASI). Results The mRNA expressions of IFN-gamma receptor and TNF-alpha receptor were observed in the PBMCs of all subjects. The mRNA expression levels of IFN-gamma receptor were 0.72 ± 0.17 in healthy controls, 1.11 ± 0.55 in all patients with psoriasis, 1.13 ±0.57 in patients with active psoriasis and 1.03 ± 0.52 in patients with stable psoriasis, respectively. A signifi-cant increase was observed in the expression levels of IFN-gamma receptor mRNA in all psoriatic patients and in patients with active psoriasis compared with those in healthy controls (both P < 0.05), but there was no significant difference between the healthy controls and patients with stable psoriasis (P ＞ 0.05). The expres-sion levels of TNF-alpha receptor mRNA were 2.05 ± 1.34 in healthy controls, 2.70 ± 3.80 in all psoriatic patients, 2.90 ± 4.40 in patients with active psoriasis, 2.14 ± 1.05 in patients with stable psoriasis, respectively;there was no significant difference between psoriatic patients and healthy controls (P ＞ 0.05). However, no correlation was found between the mRNA expression of IFN-gamma receptor, that of TNF-alpha receptor,and disease severity in psoriatic patients. Conclusions The mRNA expression of IFN-gamma receptor in PBMCs is up-regulated in patients with psoriasis vulgaris, which is unrelated to the activity of psoriasis.