硒在癌症化疗中的作用

一九五七年 Schwarz 和 Foltz 证明硒是一种保护因子,硒是动物体内必需的微量元素。近几十年的流行病学和实验研究,学者     

癌化学治疗研究的新动态

不少学者指出治疗学的第二次革命已经开始。如果说第一次治疗学革命以 Aspirin,磺胺类,抗菌素,治疗精神病药物,抗炎药物及β—阻断剂的发现为里程碑的话,第二次治疗学革命则以抗病毒药,抗肿瘤药的不     

Recent progress in the study of anticancer drugs originating from plants and traditional medicine in China.

Recent progress in the studies of anticancer drugs originating from plants in China is reviewed in this paper. Guided by the experience of traditional Chinese medicine (TCM), several new anticancer drugs have been found. Indirubin from indigofera tinctoria (青黛) is useful for the treatment of chronic myelocytic leukemia. On the basis of structure-activity relationship studies of these compounds, a second generation drug (N-methyl isoindigotin) has been developed. Clinical studies demonstrated that it is more effective than indirubin, 1O-hydroxy campothecin derived from Camptotheca acuminata (喜树) have exhibited definite activity on rodent tumors and it is used for phase Ⅱ studies. Iris latea pallasi Fischer var. Chinensis (马蔺子) may serve as     

流行性出血热所致自发性肾破裂一例报告

<正> 近年来流行性出血热所致肾破裂的报道国内外日渐增多。我院于1984年收治一例,现报道如下: 患者男,20岁,因发冷发热头痛,渐进性全腹痛转为左上腹痛6天,无尿1天,急诊入院。查体:T36.3℃;P102次/分,BP80/50,贫血外貌。神清、表情淡漠。咽红,腹胀、全腹压痛,以左上腹显著且有反跳痛,移动性浊音阳性,肠鸣音弱,左肾区叩击痛阳性、腹穿有血性液体抽     

EFFECTS OF S86019, AN ACTIVE COMPONENT FROM PURALIA LOB AT A, ON CELL DIFFERENTIATION AND CELL CYCLE TRAVERSE OF HL-60 CELLS

The effects of S86019, an active component from Puralia lobata, on the induction of cell differentiation and cell cycle traverse of HL-60 cells were described. It was shown that cell proliferation of HL-60 cells was inhibited by S86019 in vitro. Under the action of S86019 the HL-60 cells were induced to differentiate into metamyelocytes, myelocytes and much matured cells with banded or segmented nucleus. Flow cytometry demonstrated that the cell population of HL-60 cells was blocked at G1 phase which resulted in the elevation of percentage of G1 cells and decrease of percentage of cells in S phase. Experimental results demonstrated that S86019 is an active inducer of cell differentiation in HL-60 cells.     

第三代新维甲类化合物R9158对人急性早幼粒白血病NB_4细胞的分化诱导作用

目的　 为了研究本所合成的第三代新维甲类化合物 3′,5′,二叔丁基 - 4′-甲氧基 - 4 -羧基查尔酮酸 ( R91 5 8)对 NB4细胞的分化诱导作用。方法　 从形态、功能及染色体核型等方面观察 R91 5 8对 NB4细胞的影响。结果　 发现 R91 5 8与全反式维甲酸( RA)相似 ,可明显抑制 NB4细胞的生长及克隆形成 ,且在相同浓度下 R91 5 8的作用强于 RA。用 1 0 - 6mol/ L R91 5 8处理 NB4细胞 ,发现随作用时间的延长 NBT还原能力也明显增强 ,处理 6 d时 NBT阳性率为 97.5 %。用 R91 5 8与 RA处理 NB4细胞 ,NBT还原的 ED50 分别为 3.9× 1 0 - 9及 2 .8× 1 0 - 9mol/ L。形态学检查表明 ,用 1 0 - 6mol/ LR91 5 8及 RA诱导 2 d,可使细胞缩小 ;处理 6 d,细胞向成熟方向分化 ,但大多数细胞处于中幼及晚幼粒细胞阶段。染色体核型分析表明 ,NB4细胞具有亚四倍体型染色体核型 ,用 R91 5 8处理可使其非整倍体染色体数目下降。从对照的 80 .85± 7.5降至72 .96± 6 .9( P<0 .0 1 )。G带显示 ,用 1 0 - 6mol/ L R91 5 8及 RA处理 NB4细胞 6 d,其典型的 t( 1 5 ;1 7)易位未见消失。结论　 R91 5 8对 NB4细胞具有显著的分化诱导作用。     

单细胞DNA损伤的微凝胶慧星电泳检测法

单细胞ＤＮＡ损伤的微凝胶慧星电泳检测法袁守军韩锐（中国医学科学院、中国协和医科大学药物研究所，北京１０００５０中国图书分类号Ｒ３４；Ｒ３９２．２３外来理化因素对细胞ＤＮＡ的损伤是引起基因突变及癌症发生的重要因素。建立快速灵敏的ＤＮＡ损伤检测方法，对于...     

抗肿瘤药的筛选方法及策略

肿瘤化疗的长足进步起源于新抗肿瘤药的不断发现。G.Elion及G.Hitchings被授与1988年度诺贝尔医学奖一事说明抗肿瘤药研究的历史性成就已得到     

EFFECTS OF GENISTEIN ON INVASION AND MATRIX METALLOPROTEINASE ACTIVITIES OF HT1080 HUMAN FIBROSARCOMA CELLS

Effects of genistein on invasion and matrix metalloproteinase activities were investigated in HT1080 human sarcoma cells, lnvasion of HTI080 cells through reconstituted basement membnme was inhibited when the cells were treated with 100μmol/L and 200μmol/L genistein; At the same concentrations,genistein not only suppressed latent forms of matrix metalloprotinese-2 and-9 (MMP-2 mad MMP-9) to convert into active forms, but also increase dramatically the tissue inhibitor of metalloproteinase (TIMP-1 ) mRNA contents and reverse the imbalance of MMPs and TIMPs. However, expressions of MMP-2 and MMP-9 were not sigrdficantly affected. Suppression of MMP activation and increase of TMP-1 expression will decrease matrix degradation by MMPs, and consequently inhibit invasions of the cells. These results emphasized the existence of the imbalance between MMPs and TIMPs in tumor invasion mad metastasis formation, The value of genistein as a drug for antiinvasion and anti-metastasis chemotherapy was suggested.     

维A类预防癌的新进展

自1983年"维生素A类在预防肿瘤方面的意义"发表以来,短短五年中,关于维A类预防癌的研究发生了令人鼓舞的变化.新合成的维A类化合物已超过2000个,一些已进入临床试用阶段,并已获得初步的良好效果.维A类预防癌的作用机理虽然还不十分清楚,但随着研究的深入发展,已提出以蛋白激酶-C信息传递系统(PK-C,Cascade system)为代表的假说,在分子水平阐明维A类的各种生物作用.结构类型的改变,也远远超出经典的