AIDS合并马尔尼菲青霉病患者外周血Dectin-1、炎症细胞因子及CD4+T淋巴细胞表达情况及其临床意义

目的 探讨AIDS合并马尔尼菲青霉菌病(PsM)患者模式识别受体Dectin-1、炎性细胞因子及CD4+T淋巴细胞表达情况及其临床意义.方法 将120例AIDS患者根据是否合并PsM分为PsM组和非PsM组各60例,比较两组Dectin-1的平均荧光强度、CD4+T淋巴细胞计数和白细胞介素(IL)-2、IL-6、IL-12、IL-23水平.结果 PsM组的Dectin-1表达、CD4+T淋巴细胞计数、IL-2、IL-6、IL-12 和IL-23水平均低于非PsM组(P<0.05).结论 Dectin-1及其调节产生的炎症细胞因子、CD4+T水平下降可能是导致AIDS的患者对马尔尼菲青霉菌易感的重要原因.     

十二指肠镜下乳头括约肌切开术治疗胆总管结石10例临床分析

随着内镜技术和器械的进步，十二指肠镜下乳头括约肌切开术治疗胆总管结石已发展为一种成熟的技术，我们近年开展此项技术，取得较好疗效，报告如下。     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

老年人三重癌一例

患者男性,75岁。1999年发现左大腿皮下肿块,初为黄豆大小,无明显不适,未予处理;2002年3月皮下肿块增至花生大小,无红肿疼痛,在外院行切除手术,术后病理结果:恶性纤维组织细胞瘤,免疫组化:CD68(+),VIM(+),MggLobiim(±),Des(-),HMB45(-)。X 线胸片及肺 CT 检查结     

转化生长因子-β1基因多态性与结直肠癌关系的研究

目的:研究转化生长因子β1(TGF-β1)基因启动子多态性各等位基因及基因型在结直肠癌患者中的分布频率,初步分析基因型及血清水平与结直肠癌的相关性。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,检测120例结直肠癌患者和130例正常对照组TGF-β1的基因多态性,包括TGF-β1基因启动子-800G/A、-509C/T位点,同时采用酶联免疫吸附试验(ELISA)检测血清TGF-β1水平。结果:结直肠癌患者血清TGF-β1水平显著高于对照组(P<0.01)。TGF-β1基因-800G/A位点多态性在结直肠癌组和正常人群中的分布差异无显著性(P>0.05);而TGF-β1基因-509C/T多态性各等位基因及基因型频率在两组人群中的分布差异存在显著性(P<0.05),等位基因频率的相对风险分析发现,T等位基因携带者患结直肠癌的风险是C等位基因的1.580倍(OR=1.580,95%C I:1.109~2.251)。携带T等位基因的结直肠癌患者血清TGF-β1水平显著高于不携带者(54.77±12.65 ng/mLvs44.29±10.24 ng/mL,P<0.01)。结论:TGF-β1基因-509C/T多态性与结直肠癌的发病具有相关性,携带T等位基因的个体可能通过促进TGF-β1的高度表达进而增加了结直肠癌的发病风险。     

大肠癌COX-2和BFGFR表达与Dukes分期,淋巴结转移之间的关系

目的:研究COX-2、BFGFR在大肠癌中的表达和相互作用,以及它们与大肠癌Dukes分期,淋巴结转移之间的关系。方法:采用免疫组化SP法检测了49例手术切除的大肠癌及20例大肠增生性息肉组织中的COX-2、BFGFR表达。结果:COX-2、BFGFR在大肠癌中的表达阳性率分别为59.2%、65.3%,而增生性息肉的表达率分别为30.0%、35.0%,COX-2、BFGFR在大肠癌组织与增生性息肉中表达均有显著性差异。COX-2、BFGFR在大肠癌中表达分别与肿瘤Dukes分期、淋巴结转移之间具有显著性差异。大肠癌组织中COX-2、BFGFR表达二者间无相关性。结论:大肠癌组织中的COX-2、BFGFR表达水平增高,参与了大肠癌的发生发展过程,与大肠癌的预后有关,但二者的表达无相关性,提示它们在大肠癌的发生发展中所起的作用不同,是相互独立的作用因子。     

大肠癌和大肠腺瘤COX-2和BFGFR的表达及意义

目的研究COX-2和BFGFIL在大肠癌及大肠腺瘤组织中的表达及意义。方法采用SP免疫组化方法检测49例手术切除的大肠癌及25例大肠腺瘤性息肉，20例正常大肠黏膜标本组织中的COX-2、BFGFIL表达。结果COX-2、BFGFIL在大肠癌中的表达阳性率分别为59．2％和65．3％，COX-2、BFGFR在腺瘤性息肉的表达率为52．0％和60．0％，COX-2、BFGFIL在肠癌组织与腺瘤性息肉中表达均差异无显著性（P〉0．05）；正常肠黏膜中未检出COX-2、BFGFIL。COX-2、BFGFIL在大肠癌中表达与性别、年龄、肿瘤大小、肿瘤位置和分化程度无关（P〉0．05）。但与肿瘤Dukes分期和淋巴结转移之间具差异有显著性（P〈0．05）。大肠癌组织中COX-2、BFGFIL表达两者间无相关性（P〉0．05）。结论COX-2、BFGFR在大肠癌组织及大肠腺瘤中的表达水平增高，在大肠腺瘤恶变及大肠癌的发生发展过程中起重要作用，大肠癌组织中的COX-2、BFGFR表达无相关性。     

利用网络开展病人医学电子信息服务初探

我院于2005年建立医院网站,利用网络开展病人医学电子信息服务,通过给病人提供有关的医学知识、疾病诊疗、康复等医学信息服务,对改善患者的身心健康等均具有不可低估的作用.