经氯硝柳胺浸泡后湖北钉螺脑神经节的超微结构变化

用透射电镜进一步观察了湖北钉离神经节的纤维鞘，神经节胞体区和神经纤维网的超微结构，并且在神经纤维网部位首先发现了两个典型突触，经氯硝柳胺浸泡后，钉螺脑神经节的超微结构发生改变，神经纤维，神经胶质细胞和神经细胞内的线粒体由变性至坏死，神经细胞内的的糖原有含量显著减少，神经纤维网也发生变化坏死，这为阐明氯硝柳胺的灭螺机制提供了有用的资料。     

老年全身麻醉患者血清ADP、S-100β蛋白及HIF-1α水平与术后认知功能障碍的相关性分析

目的探讨老年全身麻醉患者血清脂联素(ADP)、S-100β蛋白及缺氧诱导因子-1α(HIF-1α)水平与术后认知功能障碍(POCD)的相关性。方法选取南阳市中心医院2017年1月至2019年1月收治的159例接受手术治疗的老年患者,根据术后简易精神状态检查表(MMSE)评分分为POCD组(MMSE<27分,n=53)和非POCD组(MMSE≥27分,n=106),比较两组患者手术前后ADP、S-100β蛋白、HIF-1α水平变化,Pearson相关性分析MMSE评分与血清ADP、S-100β蛋白、HIF-1α水平的相关性,受试者操作特征(ROC)曲线分析血清ADP、S-100β蛋白、HIF-1α水平对老年全身麻醉POCD的预测价值。结果术后两组血清ADP水平和MMSE评分均明显降低,S-100β蛋白、HIF-1α水平明显提升,POCD组较非POCD组改变更明显(P<0.05);Pearson相关性分析显示,POCD组术后MMSE评分与血清ADP水平呈正相关,与S-100β蛋白、HIF-1α水平呈负相关(P<0.05);ROC曲线显示,ADP+S-100β蛋白+HIF-1α预测老年全身麻醉患者POCD的曲线下面积(AUC)、敏感度、特异度高于各指标单独预测。结论老年全身麻醉POCD患者血清ADP水平明显降低,S-100β蛋白和HIF-1α水平明显提升,与POCD发生密切相关,联合检测能提升POCD预测价值。     

IMMUNOLOGICAL STUDIES ON THROMBOANGIITIS OBLITERANS

Using humoral immunity (gamma-globulin 18 cases, IgG, IgA, IgM each 30 cases, CH50, C3, C4 each 29 cases and CIC 31 cases), cellular immunity (E-RFC 68 cases, As 30 cases, SmIg, SmIgG each 18 cases, IMIT 30 cases) and immunopathological manifestations (light microscope, fluorescence microscope, transmitted electron microscope each 7 cases) as indices, we studied the immunological changes of "progressing", "remittent" and "stabilized" groups of patients with thromboangiitis obliterans (TAO) in different stages. Humoral immunity indicated that gamma-globulin, immune complex and IgG were all increased; cellular immunity indicated that the rate of T cells and suppressor cells was declined, while that of B cells was elevated. Immunopathologically, under light microscope all the layers of involved vessels were infiltrated with neutrophils, lymphocytes and monocytes; under fluorescence and electron microscope, immune complexes were found in the involved vessel walls. Our preliminary results suggest that TAO is an autoimmune disease relevant to antigen-antibody complex.     

TREATMENT OF HYALINEMEMBRANE DISEASE BYHUMAN AMNIOTIC FLUIDSURFACTANT

A case of hyaline membrane disease was treated successfully with pulmonary surfactant (PS) isolated from human amniotic fluid. Dosage was 150 mg phospholipid/kg. The exogenous surfactant was instilled into the airway via a tracheal cannula. Clinical symptoms, PO2 and FiO2 improved evidently 24 hours after administration. L/S ratio and phosphatidylglycerol recovered gradually in aspirates. Lung X-ray film manifested "white lung" before instillation of surfactant and showed a striking improvement 3 days after treatment. The duration of mechanical ventilation was 6 days. During the period of recovery complications of patent ductus arteriosus and bacterial pneumonia developed. However, the patient recovered completely and was discharged 32 days after admission.     

呼吸道病毒感染多重、快速检测技术

众多资料显示急性呼吸道感染中90％～95％是由病毒感染所致，其中多种病毒具有感染力强、传播快、潜伏期短、发病急等特点，并且近来新的致病性病毒还在不断地被发现，如人类偏肺病毒、SARS病毒、高致病性禽流感病毒等，给临床疾病诊断和治疗造成极大的困难。     

碘缺乏病区儿童智力水平的研究

本文研究了四川省开江县碘缺乏病区儿童智力水平及智商与学业成绩的关系。结果表明碘缺乏对儿童智力发育确实存在一定影响，病区儿童智商明显低于非病区。同时碘缺乏所造成的亚临床损伤是多样的。     

消化道肿瘤自身抗体检测

目的探讨消化道肿瘤患者针对肿瘤细胞的自身抗体的存在。方法收集肠癌患者及胃癌患者术前血清96份和健康体检人群血清62份，分组后采用Western印迹分析法定性检测消化道肿瘤患者术前血清中的自身抗体。结果正常健康人群和正常肝细胞株96％（212／220）的反应条无显色带，而97％（60／62）的肠癌患者肠癌细胞株、89％（39／44）的胃癌患者胃癌细胞株反应条均出现多个阳性带。结论健康人群体内很少产生针对肿瘤细胞的自身抗体，而大部分消化道肿瘤患者体内产生针对肿瘤相关抗原的自身抗体，这为消化道肿瘤的病因，早期诊断，预后监测及分子靶向治疗等研究提供了一个很好的线索。     

放射治疗对乳腺癌患者外周血免疫系统相关指标的影响

目的:研究分析放射治疗对乳腺癌患者免疫系统相关指标的影响。方法:选取在医院就诊的6例病理诊断为乳腺癌Ⅱ~Ⅲ期患者,将所有患者放射治疗前(0 Gy)静脉血样本定义为对照组、放射治疗20 Gy和50 Gy照射后静脉血样本分别定义为20 Gy组和50 Gy组,于放射治疗10次后、25次后采集不同放射治疗累积剂量24 h后静脉血8 ml;采用流式细胞术检测所有受检者不同剂量放射治疗后外周血淋巴细胞亚群百分数、外周血淋巴细胞凋亡及细胞周期的改变;实时荧光定量聚合酶链反应(q RT-PCR)法检测外周血淋巴细胞微小核糖核酸(miRNA)的改变。结果:乳腺癌患者20 Gy组和50 Gy组照射后与对照组比较,淋巴细胞亚群CD3⁺、CD4⁺/CD8-和CD4⁺/CD8⁺表达水平均呈下调趋势,CD4^-/CD8⁺呈上升趋势,但差异均无统计学意义;20 Gy组T细胞抗原受体(TCR)/CD3明显下调,与对照组比较差异有统计学意义(Z=-2.008,P<0.05),50 Gy组TCR/CD3有所回升,但差异无统计学意义。不同剂量的两组照射后乳腺癌患者mi RNA-150、mi RNA-210表达水平随剂量增加呈降低趋势,50 Gy组表达水平明显降低,与对照组相比差异有统计学意义(Z=-2.242,Z=-2.402;P<0.05)。结论:放射治疗未引起乳腺癌患者明显的免疫功能抑制,可能是通过改变mi RNA-150、mi RNA-210的通路而抑制肿瘤的生长。