人脐带间充质干细胞获得和纯化及向软骨细胞分化能力的研究

Objective To explore the efficient way to purify and to enrich umbilical cord mesenchymal stem cells which have abilities to differentiate into chondrocytes. Methods Umbilical cord was acquired from new born infants and chopped into 1 mm2 cubics, then they were pretreated with colleganase and trypsin so as to get the mixed cell suspension. CD45(-),CD90(+) cells were acquired from the mixed cell suspension via FCM sorting and were cultured in the medium as umbilical cord mesenchymal stem PO cells. The PO cells were detached and passaged by multiplex collagenase NB4 and the positive rate of CD90 and CD45 of the P1-P3 cells were observeed via FCM ; the P3 cells were induced to differentiated into chondrocytes. Results FCM results showed that the positive rate of CD90 on P1, P2, F3 was (80.86 ± 7.85)%,(95.86± 3.28)%,(97.15± 1.43)%, while the positive rate of CD45 on P1, P2, P3 cells was (2.53 ±0.28)% and (0.97 ± 0.48)% and (0.05 ± 0.01)% respectively; P3 cells had differenciated into chondrocytes. Conclusion FCM presorting and Multiplex collagenase detachment procedures can swiftly acquire highly purified human umbilical cord mesenchymal stem cells which have the ability to differentiate into chondroeytes.     

脐带间质干细胞的研究进展

间充质干细胞（mesenchymal stemcells,MSCs）最初是特指骨髓基质细胞,后来研究发现在胎儿肝脏、肺脏、心脏等实质脏器及脐带血中均提取到了与骨髓基质细胞生物学特性相似、免疫表型相同的干细胞,因此将间质组织来源的与骨髓MSCs生物学性状相似,具有多向分化潜能的细胞统称为间充质干细胞。     

小儿阑尾炎误诊分析

目的分析小儿阑尾炎误诊原因，降低小儿阑尾炎的误诊率，减少术后并发症及阑尾炎误切的发生。方法回顾我院及外院近100年首诊误诊的155例急性阑尾炎的临床资料，对病史、相关检查结果、手术所见进行回顾性研究，分析误诊原因。结果术前诊断为其他疾病，手术确诊为阑尾炎131例；术前诊断为阑尾炎，手术证实为其他疾病24例。手术后均痊愈出院，并发症的发生率为14．27％。结论对小儿急性阑尾炎的临床症状特点缺乏足够的认识，腹部体征的掌握不准确及过多依赖辅助检查是误诊的主要原因。小儿不会准确地表述临床症状，小儿神经系统发育不健全，早期即出现发热、胃肠道症状，多以发热、呕吐为首发症状就诊故易延诊、误诊，年龄越小越突出。耐心细致，反复检查右下腹有无压痛，对诊断有决定性的意义。阑尾炎确诊后应及早手术治疗，对酷似阑尾炎的病例，应当允许阑尾炎误切的存在，但应注意诊断和鉴别诊断，尽量减少误切。     

龚氏四步复位法术前复位对旋后外旋型Ⅳ度踝关节骨折围手术期疗效的影响

目的:观察龚氏四步复位法术前复位对旋后外旋型Ⅳ度踝关节骨折围手术期疗效的影响.方法:2018年1月至2020年1月期间完成治疗的旋后外旋型Ⅳ度踝关节骨折患者30例,予以龚氏四步复位法复位、石膏固定、手术治疗,分析患者围手术期患肢疼痛VAS评分、术后踝关节功能AOFAS评分、张力性水泡、切口皮缘坏死渗液等并发症情况.结果...     

手的整形美容

手是人类进化的产物,是创造世界文明的特殊劳动工具,也是人的“第二张脸”。因此,手的修复不但要恢复其功能和外形,还要有美容的效果。经过半个多世纪的发展,在老一辈手外科和整形外科专家的努力探索下,我国手外科从无到有逐渐发展壮大,并在皮瓣外科、断指再植、足趾游离移植、