Alterations in hemorheological properties of erythrocytes in nude mice with erythroleukemia and the treatment effects of etoposide

The purpose of this study was to examine the changes of hemorheological properties of erythrocytes in the nude mice with erythroleukemia and the treatment effects of etoposide (VP16). Thirty mice were randomly divided into three groups: the control group (C group), injected with 1 ml saline solution, the MEL group (M group) injected with 1 ml MEL (murine erythroleukemia cell line) and the MEL + VP16 group (V group) injected with 1 ml MEL and from the 8th day after injection, 20 microl VP16 (1 microg/microl) was injected intraperitoneally every five days. One week after MEL injection, erythroblastic cells increased in the bone marrow and proerythroblasts were found in the peripheral blood, suggesting that erythroleukemia was induced. Abnormalities were also found in spleens and livers later. At around twenty days after injection, the mice in M group died and about four weeks after injection, the mice in V group also died. Compared with C group, the hemorheological indexes [the deformation index DI, orientation index (DI(or)), and the small deformation index (DI(d))], electrophoretic mobility, membrane fluidity as well as osmotic fragility of red blood cells (RBC) in M and V groups changed significantly. But after VP16 administration, the changes of above parameters in V group were less significant than those of M group. The results above suggested that intraperitoneal injection of MEL cells could cause erythroleukemia in nude mice, VP16 could alleviate the erythroleukemia symptom and improve the hemorheological properties, and could prolong V group nude mice survival.     

生长抑素和全肠道外营养治疗先天性乳糜腹一例

先天性乳糜腹在新生儿时期并不常见,其病因尚不十分清楚。先天性乳糜腹的发生主要与淋巴管先天发育异常等因素有关。2004年．我院收治1例先天性乳糜腹患儿,现报告如下。     

幼儿抗心磷脂抗体阳性伴活化部分凝血活酶时间延长一例

患儿男,3岁。因患隐睾准备接受手术治疗。术前检查时发现除活化部分凝血活酶时间(APTT)延长外,其他均正常。患儿发育正常,无鼻衄、牙龈出血,无四肢、关节肿胀等。未曾患过肝炎等相关疾病,父母及家系均无出血性疾病或遗传病史。体检：发育良好,无贫血貌,全身皮肤未见紫癜或出血点,四肢关节正常,左侧睾丸未降至阴囊,心肺正常,肝脾未及。     

多药耐药基因在慢性癫癎大鼠中的表达及托吡酯对其表达的影响

目的探讨多药耐药基因（mdr）在慢性大鼠中的表达及托吡酯（TPM）对其表达的影响。方法将生后28d的SD大鼠给予海人酸致,对照组给予相同的方法注射生理盐水。待自发性形成后,将癫痫组分为持续状态并发自发性（简称SE）组、持续状态并发自发性治疗（简称SE＋TPM）组、非癫痫持续状态并发自发性癫痫（简称N—SE）组和非持续状态并发自发性治疗（简称N—SE＋TPM）组;将生理盐水对照组分为正常生理盐水对照组和正常生理盐水对照治疗（对照＋TPM）组。治疗组均给予TPM治疗,治疗6周后,将所有大鼠断头取海马,RT—PCR检测mdr1α和mdr1b mRNA的表达。结果SE组、SE＋TPM组和N—SE＋TPM组的mdr1α和mdr1b mRNA表达均比对照组高（P〈0．001或〈0．05）,SE＋TPM组的mdr1α和mdr1b mRNA表达也比SE组高（P〈0．001）,其他治疗组（N—SE＋TPM,对照＋TPM）mdr1α和mdr1b mRNA表达较相对应的未治疗（N—SE,对照）组相比高,但无统计学意义（P〉0．05）。结论反复癫痫发作,特别是持续状态可使大鼠海马中mdr1α和mdr1b mRNA表达增加,颞叶的耐药可能与mdr1α和mdr1b mRNA高表达有关。TPM可增加大鼠海马中mdr1α和mdr1b mRNA的表达,尤其mdr1α mRNA的表达更明显。mdr1α 和mdr1b mRNA高表达可能与癫痫和TPM均有关。     

以腹泻、喘憋起病的纵隔囊肿一例

患儿男,3个月,因腹泻伴喘憋2天于2004年7月23日人院。2天前患儿无明显诱因出现腹泻,黄绿色稀水样便,每日3～5次,伴腹胀,无发热。腹胀时哭闹明显,出现喘憋,呼吸困难,哭闹剧烈时口唇发绀伴喉鸣,犬吠样咳嗽。当地给予强心利尿治疗无好转。患儿生后半个月出现喉鸣,吃奶无呛咳,预防接种按时按序。     

Human neutrophil lipocalin (HNL) as a diagnostic tool in children with acute infections: a study of the kinetics

AIM: To study the changes in blood of human neutrophil lipocalin (HNL) and C-reactive protein (CRP) during the course of an acute infection in children. METHODS: Children (n=92) hospitalized with symptoms and signs of acute infections were included and categorized into five groups, i.e. bacterial infection, suspected bacterial infection, viral infection, suspected viral infection and others. Blood was taken at admittance and the following 3-4 d for the measurement of CRP and HNL. RESULTS: Both CRP and HNL were significantly raised at admittance in bacterial infection as compared to viral infection (p<0.001). After 25-48 h, 83% of the children with bacterial infections still had raised CRP levels in contrast to 11% having raised HNL levels. The levels of CRP, but not those of HNL, were significantly correlated to days of symptoms before admission. CONCLUSIONS: HNL is a promising diagnostic tool in the distinction of acute infections caused by bacteria or virus. The differences in the kinetics of CRP and HNL make HNL a better marker for monitoring antibacterial treatment, since HNL is probably elevated only when an active bacterial infection is at hand.