人促红细胞生成素基因表达的研究

为进一步探讨运用促红细胞生成素基因治疗肾性贫血的依据，本研究将人ＥＰＯｃＤＮＡ构建到逆转录病毒载体ＬＸＳＮ中，然后将重载体转染到２包装细胞系ＰＡ３１７中，经选择培养后，运用ＤＮＡ－ＰＣＲ，ＲＴ－ＰＣＲ，原位杂交ＥＰＯ生物活性检测，观察ＥＰＯｃＤＮＡ和ＬＸＳＮ－ＥＰＯ在ＰＡ３１７中的表达。     

血清白蛋白水平对腹膜透析患者残余肾功能的影响

Objective To study the effect of serum albumin(Alb) levels on residual renal function (RRF) in peritoneal dialysis patients. Methods Fiftyfour patients accepting peritoneal dialysis were divided into two groups base- d on the serum levels of Alb,including group A (Alb≥35 g/L) and group B(Alb < 35g/L). The serum levels of Alb, creatinine, urea nitrogen, IL-6, TNFα were determined. 24 - hour urine and peritoneal dialysis fluids were remained and urea nitrogen, creatinine, RRF, and others were determined. The above mentioned marks and RRF were observed in follow- up visit. Results The proportion of diabetic nephropathy patients in group B was higher than in group A. Correlative analysis indicated that a negative correlation was found between the levels of Alb and mediators of inflammation IL - 6 and TNF - α. The de-crease in RRF is time- dependent in all selected patients. But the RRF in group B began to decline since peritoneal dialysis 6th month, there is lower compared with 1st month. After peritoneal dialysis 12th months the RRF in group A also significant declined, but the degree of declining in group B was greater. Correlative analysis indicated that the negative correlation was found between RRF and the levels of Alb, otherwise the positive correhtion was found between the RRF and mediators of inflammation IL - 6 and TNF - α. Conclusions The low levels of Alia resulted from malnutrition was one of the factors to decline RRF in per-itoneal dialysis patients. The high levels of mediators of inflammation accelerated the deterioration of the RRF.     

沙库巴曲缬沙坦治疗终末期肾病合并射血分数降低心力衰竭1例

心力衰竭是终末期肾病(End-stage renal disease,ESRD)常见并发症,它严重影响患者的生存质量及预后,也给个人和社会造成极大的经济负担,故而改善ESRD患者心力衰竭显得尤为重要和迫切.慢性心力衰竭传统治疗药物一般是指血管紧张素转换酶抑制剂(Angiotensin converting enzyme...     

血液透析治疗10例流行性出血热肾衰竭疗效观察

目的：探讨血液透析在流行性出血热合并急性肾功能衰竭中的积极意义。方法对我院2008年1月-2013年8月期间10例流行性出血热合并急性肾功能衰竭患者使用血液透析治疗,同时予以相应的基础治疗,观察临床效果。结果治愈6例,3例明显好转,1例自动出院,10例患者临床症状均得以缓解,治疗前后血尿素氮、血肌酐显著降低,差异有统计学意义（P〈0.05）;血小板显著上升,差异有统计学意义（P〈0.05）。结论血液透析治疗流行性出血热合并急性肾功能衰竭疗效肯定,值得临床广泛应用。     

CAPN-10基因与腹膜透析患者代谢综合征危险因素的关系

目的 探讨CAPN-10基因Indel-19与腹膜透析患者代谢综合征(MS)危险因素的关系.方法 用PCR-RFLP(聚合酶链反应-限制性片断长度多态性)法检测30例健康者(A组)、30例稳定的腹膜透析患者(B组)CAPA-10基因Indel-19多态性,生化技术测定FBG(空腹血糖)、TG(三酰甘油),HDL-C(高密度脂蛋白胆固醇)及FINS(空腹胰岛素)水平.用HOMA-IR法评价胰岛素抵抗,同时对每个受试对象进行MS危险因素评估.结果 B组患者收缩压(SBP)、舒张压(DBP)、FBG、FINS、TG及HOMA-IR水平较A组高(P＜0.05).A、B两组比较CAPN-10基因Indel-19基因型分布无明显差异(P=0.580).B组基因型为2/2的患者较基因型为1/1、1/2患者有较多MS的危险因素.结论 腹膜透析患者多同时存在胰岛素抵抗、脂质代谢紊乱及高血压.CAPN-10基因Indel-19可能与腹膜透析患者血糖、血脂及高血压有关.其可能机制是影响腹膜透析患者血糖、脂质代谢,加重患者胰岛素抵抗的程度,但其具体作用机制尚有待于进一步研究.