细胞荧光素酶报告基因试验受CpG岛甲基化状态影响

Objective To investigate the effects of methylation status of CpG islands of endogenous E-cadherin (CDH1) gene on the promoter activity of corresponding genes in reporter assays. Methods The methylation statuses of CpG island of CDHI in 8 different cell lines were detected by methylation-specific PCR. CDH1 protein was analyzed by Western blotting. Two sets of pGL3 reporter vectors with different genotypes/haplotypes of the CDH1 promoter were constructed [pGI3-A(-73)/-C(-73)pGL3-H1/-H4]and used to transfect these cell lines. The differences between these promoter reporter vectors were analyzed by t-test. Results (1) CDH1 CpG island was unmethylated in AGS, MCF7, MKN74, and PC-3 cell lines,expressed in MCF7, MKN74, and PC-3 ,but not in AGS. Expression of CDH1 was silenced by methylation in HeLa, BGC823, A549, and RKO cell lines. (2) In the four CDH1 -unmethylated MCFT, M KN74, PC-3, and AGS cell lines ,the promoter activities of pGI3-C(-73)(as 0. 78±0. 10,0. 17±0.01,0. 11±0. 01,1.19±0. 18)were significantly higher than those of pGL3-A(-73)(as 0. 30±0. 08,0. 07±0. 01,0. 07±0. 01,0. 39±0. 04) (t values are -6. 298, -12. 349, -8. 128, -7.388, and P<0. 01). However, in the four C DH1 -methylated HeLa, BGC823, A549, and RKO cell lines, the promoter activity of pGL3-C(-73)(as 0. 09±0. 02,0. 13±0. 02,0. 05±0. 01,0. 01±0. 00) was significantly lower than that of pGL3-A(-73)(as 0. 16±0. 01,0.25±0.01,0. 11±0.03,0.03±0.00) (t valued at 5.958,11. 189,3. 661,13. 866,and P<0.05). (3) In the unmethylated MKN74 and methylated RKO cell lines, the promoter activities of pGI3-H1/-H4 were obviously and contrarily different(as 1.57±0. 23/0. 94±0. 06 and 0. 38±0. 02/0. 50±0. 04 ,t values were 4. 577 and -4. 915 ,P values were 0. 010 and 0. 003). Conclusion The methylation status of CpG island of the target gene in the tested cell lines affects the promoter activity in Reporter Assay significantly. The most active one may be the most suppressive one.     

100例鼻咽癌患者心理社会因素配对调查与多变量分析

为探讨心理社会因素对恶性肿瘤的影响规律，本文按多因素１：１病例－配对对照设计法，对１００例鼻咽癌及其配对对照了临床流行病学调查。资料经ＱＵＩＣＫ ＢＡＳＩＣ语言支持下完成了配对Ｌｏｇｉｓｔｉｃ回归分析。在α＝０．１０的水准，分别有Ｘ１，Ｘ２，Ｘ３，Ｘ４，Ｘ６，Ｘ１０，及Ｘ１１共７个入选的变量最终进入了方程：Ｌｏｇｉｔ Ｐ＝ａｉ＋０．２８８Ｘ１＋０．５６０Ｘ２－１．０１５Ｘ３－１．６９５Ｘ４＋０．９     

深圳市中小学生肥胖现况及其影响因素的调查研究

对深圳市部分中小学生肥胖的影响因素进行了调查。结果表明，影响因素有：（１）家族史，在遗传因素中，母亲肥胖起着重要作用；（２）饮食习惯，尤其有常吃油腻性食物、进食速度快的习惯者。结果提示，早期预防儿童肥胖势在必行。     

鼾症与阻塞性睡眠呼吸暂停综合征

人的一生中近三分之一的时间是在睡眠中度过,入睡以后意识潜伏,感觉减退,机体与外界的主动联系暂时中断。近20年来,由于无创性血氧饱和度监测及多导睡眠图监测等先进技术应用于临床,使人们对睡眠生理及其相关疾病的认识、诊断和治疗水平进一步提高。     

选择性IgA缺乏症的部分细胞因子研究

为探讨白细胞介素及白细胞介素受体在选择性ＩｇＡ缺乏症发过程中的作用，我们采用间接免疫荥光技术ＥＬＩＳ夹心法及细胞培养，分别对１５例患儿及对照组进行了Ｔ细胞亚群，Ｔａｃ阳性细胞（ｍＩＬ－２Ｒα）百分率，ＳＩＬ－２Ｒ及ＩＬ－４含量检测，发现ＳＩｇＡＤ患儿Ｔａｃ阳性细胞（ｍＩＬ－２Ｒα百分率及血清ＳＩＬ－２Ｒ明显高于对照组，ＣＤ４，ＣＤ４／ＣＤ８，ＩＬ－４水平则显著低于对照组，表明血清ＳＩＬ－２Ｒα异常     

疗养康复护理管理效益论与思路

管理的最终目的是创造出最大的效益。因而，效益论是探讨管理功效规律性的理论和实践基础，是衡量管理工作优劣的理论标准。近年来，我们运用效益原理指导疗养康复护理实践，摸索出一条行之有效的护理管理思路，试述如下。     

Dycal自凝氢氧化钙盖髓术疗效观察

1993年以来,开始应用Dycal自凝氢氧化钙做直接盖髓剂保存活髓,取得良好效果,报告如下。1 材料和方法1.1 材料及其特点 Dycal自凝氢氧化钙是登士柏公司于1960年发明的。可用作覆盖牙髓和保护性基底。它具备自凝特性,并能形成一坚固的薄层,以保     

酒石酸美托洛尔胶囊剂与片剂体外溶出度的研究

本文采用紫外分光光度法对国内不同药厂生产的酒石酸美托洛尔胶囊剂与片剂体外溶出度进行了对比研究。将实验数据运用威布尔分布函数进行处理,求得参数m、T_(50)、Td,对体外参数T_(50)进行了方差分析和t检验。结果表明,不同剂型间有显著性差异;同一剂型不同批号的胶囊剂或片剂间无明显差异,证明处方和工艺设计合理。