调Q倍频Nd：YAG激光治疗太田痣的副反应及相关因素的探讨

观察调Q倍频Nd：YAG激光治疗太田痣的副反应及其相关因素。方法回顾性分析750例患者经调Q倍频Nd：YAG激光治疗后的副反应及其与治疗次数、治疗能量的关系。结果750例接受治疗的患者中发生副反应者76例(10．1％)，其中暂时性色素沉着55例(7.3％)，暂时性色素减退18例(2.4％)，永久性色素减退1例(0.1％)，萎缩性瘢痕2例(0.3％)。出现副反应与治疗次数无关，与治疗能量有关。结论副反应的出现与激光治疗本身没有特异性关系，随治疗能量的增加副反应发生率上升。调Q倍频Nd：YAG激光治疗太田痣无连续激光治疗后所致的增生性瘢痕、持续性色素沉着等副反应，用于治疗太田痣是安全的。     

用ROC曲线法分析99Tcm-HL91肺肿瘤阳性显像的诊断效能

目的探讨半定量分析及接受器工作特性(ROC)曲线法在99Tcm-4,9-二氮-3,3,10,10-四甲基十二烷-2,11-二酮肟(HL91)肿瘤阳性显像鉴别肺部良恶性肿块中的价值.方法经CT检查发现肺部肿块的患者50例,均经活组织检查或手术病理检查证实.根据病理检查结果分为恶性组37例和良性组13例.术前行99Tcm-HL91 2 h、4 h平面显像及4 h断层显像,分别使用视觉判断法、半定量分析及ROC曲线法分析显像结果.结果①视觉判断法99Tcm-HL91显像的灵敏度、特异性和准确性分别为97.3%、69.2%和90.0%.②肿瘤/正常肺组织(T/N)比值半定量分析及ROC曲线法恶性组2 h、4 h平面显像及4 h断层显像T/N比值分别为1.52±0.19、1.73±0.28及2.84±0.97;良性组分别为1.20±0.16、1.24±0.20及1.52±0.40.各个时相的曲线下面积分别为0.909±0.056、0.945±0.039、0.953±0.034.从99Tcm-HL91断层显像ROC曲线的界值点找到1个界点(T/N=1.76),以其作为判断良、恶性的诊断阈值,灵敏度、特异性和准确性分别为100%、84.6%和96.0%.③视觉判断法与半定量分析法比较T/N比值半定量分析及ROC曲线法使诊断的灵敏度、特异性和准确性都有提高,尤其特异性.但两种方法间差异无显著性(P均＞0.05).结论半定量分析及ROC曲线法的诊断阈值可进一步提高99Tcm-HL91肺部肿瘤阳性显像的诊断效能.     

细胞荧光素酶报告基因试验受CpG岛甲基化状态影响

Objective To investigate the effects of methylation status of CpG islands of endogenous E-cadherin (CDH1) gene on the promoter activity of corresponding genes in reporter assays. Methods The methylation statuses of CpG island of CDHI in 8 different cell lines were detected by methylation-specific PCR. CDH1 protein was analyzed by Western blotting. Two sets of pGL3 reporter vectors with different genotypes/haplotypes of the CDH1 promoter were constructed [pGI3-A(-73)/-C(-73)pGL3-H1/-H4]and used to transfect these cell lines. The differences between these promoter reporter vectors were analyzed by t-test. Results (1) CDH1 CpG island was unmethylated in AGS, MCF7, MKN74, and PC-3 cell lines,expressed in MCF7, MKN74, and PC-3 ,but not in AGS. Expression of CDH1 was silenced by methylation in HeLa, BGC823, A549, and RKO cell lines. (2) In the four CDH1 -unmethylated MCFT, M KN74, PC-3, and AGS cell lines ,the promoter activities of pGI3-C(-73)(as 0. 78±0. 10,0. 17±0.01,0. 11±0. 01,1.19±0. 18)were significantly higher than those of pGL3-A(-73)(as 0. 30±0. 08,0. 07±0. 01,0. 07±0. 01,0. 39±0. 04) (t values are -6. 298, -12. 349, -8. 128, -7.388, and P<0. 01). However, in the four C DH1 -methylated HeLa, BGC823, A549, and RKO cell lines, the promoter activity of pGL3-C(-73)(as 0. 09±0. 02,0. 13±0. 02,0. 05±0. 01,0. 01±0. 00) was significantly lower than that of pGL3-A(-73)(as 0. 16±0. 01,0.25±0.01,0. 11±0.03,0.03±0.00) (t valued at 5.958,11. 189,3. 661,13. 866,and P<0.05). (3) In the unmethylated MKN74 and methylated RKO cell lines, the promoter activities of pGI3-H1/-H4 were obviously and contrarily different(as 1.57±0. 23/0. 94±0. 06 and 0. 38±0. 02/0. 50±0. 04 ,t values were 4. 577 and -4. 915 ,P values were 0. 010 and 0. 003). Conclusion The methylation status of CpG island of the target gene in the tested cell lines affects the promoter activity in Reporter Assay significantly. The most active one may be the most suppressive one.     

100例鼻咽癌患者心理社会因素配对调查与多变量分析

为探讨心理社会因素对恶性肿瘤的影响规律，本文按多因素１：１病例－配对对照设计法，对１００例鼻咽癌及其配对对照了临床流行病学调查。资料经ＱＵＩＣＫ ＢＡＳＩＣ语言支持下完成了配对Ｌｏｇｉｓｔｉｃ回归分析。在α＝０．１０的水准，分别有Ｘ１，Ｘ２，Ｘ３，Ｘ４，Ｘ６，Ｘ１０，及Ｘ１１共７个入选的变量最终进入了方程：Ｌｏｇｉｔ Ｐ＝ａｉ＋０．２８８Ｘ１＋０．５６０Ｘ２－１．０１５Ｘ３－１．６９５Ｘ４＋０．９     

深圳市中小学生肥胖现况及其影响因素的调查研究

对深圳市部分中小学生肥胖的影响因素进行了调查。结果表明，影响因素有：（１）家族史，在遗传因素中，母亲肥胖起着重要作用；（２）饮食习惯，尤其有常吃油腻性食物、进食速度快的习惯者。结果提示，早期预防儿童肥胖势在必行。     

鼾症与阻塞性睡眠呼吸暂停综合征

人的一生中近三分之一的时间是在睡眠中度过,入睡以后意识潜伏,感觉减退,机体与外界的主动联系暂时中断。近20年来,由于无创性血氧饱和度监测及多导睡眠图监测等先进技术应用于临床,使人们对睡眠生理及其相关疾病的认识、诊断和治疗水平进一步提高。     

选择性IgA缺乏症的部分细胞因子研究

为探讨白细胞介素及白细胞介素受体在选择性ＩｇＡ缺乏症发过程中的作用，我们采用间接免疫荥光技术ＥＬＩＳ夹心法及细胞培养，分别对１５例患儿及对照组进行了Ｔ细胞亚群，Ｔａｃ阳性细胞（ｍＩＬ－２Ｒα）百分率，ＳＩＬ－２Ｒ及ＩＬ－４含量检测，发现ＳＩｇＡＤ患儿Ｔａｃ阳性细胞（ｍＩＬ－２Ｒα百分率及血清ＳＩＬ－２Ｒ明显高于对照组，ＣＤ４，ＣＤ４／ＣＤ８，ＩＬ－４水平则显著低于对照组，表明血清ＳＩＬ－２Ｒα异常