Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

652例颈内静脉置管法改良与护理临床应用研究

目的 经颈内静脉穿刺输液深静脉高营养,快速大量输液、输血,抢救休克病人,采集静脉血液标本,解决周围静脉穿刺困难,血液动力学监测,减少护理工作量,减轻病人多次穿刺的痛苦.方法 胸心外科的护理中改良颈内静脉置管法,选择在环状软骨与胸锁乳突肌内缘水平线上触及动脉搏动最明显处右外侧0.5～1cm处作为穿刺点进行穿刺置管.结果 652例颈内静脉置管,成功率达99.23%,留置时间3～25天,无血肿、气栓、气胸、局部感染、败血症等并发症发生.结论 颈内静脉穿刺置管改良方法不用刀破穿皮肤,不用扩条扩张,穿刺处不缝合,操作简单易行,成功率高,并发症少,值得推广应用.     

转化生长因子-β1基因多态性与结直肠癌关系的研究

目的:研究转化生长因子β1(TGF-β1)基因启动子多态性各等位基因及基因型在结直肠癌患者中的分布频率,初步分析基因型及血清水平与结直肠癌的相关性。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,检测120例结直肠癌患者和130例正常对照组TGF-β1的基因多态性,包括TGF-β1基因启动子-800G/A、-509C/T位点,同时采用酶联免疫吸附试验(ELISA)检测血清TGF-β1水平。结果:结直肠癌患者血清TGF-β1水平显著高于对照组(P<0.01)。TGF-β1基因-800G/A位点多态性在结直肠癌组和正常人群中的分布差异无显著性(P>0.05);而TGF-β1基因-509C/T多态性各等位基因及基因型频率在两组人群中的分布差异存在显著性(P<0.05),等位基因频率的相对风险分析发现,T等位基因携带者患结直肠癌的风险是C等位基因的1.580倍(OR=1.580,95%C I:1.109~2.251)。携带T等位基因的结直肠癌患者血清TGF-β1水平显著高于不携带者(54.77±12.65 ng/mLvs44.29±10.24 ng/mL,P<0.01)。结论:TGF-β1基因-509C/T多态性与结直肠癌的发病具有相关性,携带T等位基因的个体可能通过促进TGF-β1的高度表达进而增加了结直肠癌的发病风险。     

利用网络开展病人医学电子信息服务初探

我院于2005年建立医院网站,利用网络开展病人医学电子信息服务,通过给病人提供有关的医学知识、疾病诊疗、康复等医学信息服务,对改善患者的身心健康等均具有不可低估的作用.     

超声检查在鼻咽癌颈淋巴结转移诊断中的应用

目的探讨经颈超声检查在鼻咽癌及其颈淋巴结转移诊断中的应用价值。方法以CT作为参照,对4例病理已确诊为鼻咽癌的住院患者进行鼻咽部和左右侧颈部超声检查。患者取仰卧位,检查时头略向对侧倾斜,以腮腺及咽旁间隙作为透声窗,取下颌角和乳突间的耳垂下斜横断面和下颌骨升支旁的斜冠状切面显示鼻咽部,测量病理情况下鼻咽部软组织的厚度或肿块的范围,观察其形态、边界、内部回声及与周围组织的关系,然后再用高频探头检查左右侧颈部,并对肿大的淋巴结测量记录。结果4例患者显示6个病灶,其中单侧病灶2例,双侧病灶2例。病灶中低回声4个,中等回声2个,实质性回声6个,夹杂液无回声区1个;声像图表现鼻咽部软组织增厚4个,鼻咽部肿块2个;超声提示4例颈部淋巴结均肿大。结论经颈超声扫查可观察到鼻咽癌相应的鼻咽部软组织异常增厚或肿块的声像表现,有助于鼻咽癌颈部淋巴结转移的诊断。     

超声引导下经皮穿刺活检对浅表肺和胸膜病变的临床意义

目的探讨超声定位引导下经皮穿刺对浅表肺和胸膜病变进行活检及病理诊断,以提高超声声像图分析诊断准确性.方法对82例患者先行超声扫查,确定病变部位,记录病变形态、大小、边界、内部回声、活动度、胸膜变化等指标,消毒局麻后超声定位引导穿刺针进入病变中心,取材组织送病理组织学检查并行常规涂片细胞学检查.结果超声定位引导穿刺活检(18 G针)病理组织学诊断率达81.7%(67/82);细胞学诊断率60.9%(50/82).术后仅有2例患者2.4%(2/82)出现轻度咳嗽伴有少量血痰,经对症处理后症状消失,其余患者均无出血等并发症发生.病理诊断肺癌18例,超声显示病变呈类圆形低水平回声或中等回声;病理诊断局灶性肺炎性实变47例,超声表现为片状或楔形的低回声及双线状强回声;病理诊断肺结核瘤12例及肺炎性假瘤4例,超声显示病变均呈类圆形的低回声表现,肺结核瘤部分病变内可见强回声钙化灶;病理诊断肺错构瘤1例,超声显示病变呈欠均匀的弱回声团.结论超声引导下经皮对浅表肺和胸膜病变进行组织学穿刺活检,可快速明确诊断,超声与病理对照检查有利于提高超声声像分析诊断准确性并对临床选择和制定合理的治疗方案有重要指导意义.     

钦州市六所二级以上综合医院125项落选课题原因分析

为探讨地方医疗单位科研立项管理的经验及做法,以提高立项课题获准率,本文对1986～2006年5月期间钦州市6所二级以上医疗单位125项落选课题,进行落选原因调查分析,结果表明影响立项课题获准率的决定因素主要是课题的学术水平和项目管理两大因素.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

复方阿米卡星凝胶预防留置尿管逆行感染的研究

为探讨预防留置尿管逆行感染(UTIC)的有效措施,将100例留置尿管>10d的病人,随机分为观察组和对照组各50例,按双盲法操作.观察组应用自行研制的复方阿米卡星凝胶替代石蜡油作尿管润滑剂,置管期间,行尿道口清洁后用凝胶均匀涂于尿道口周围,2次/d;对照组按常规留置尿管及护理.比较两组病人置管期间尿细菌培养阳性病例数.结果留置尿管40d,观察组发生UTIC2例,对照组发生30例,两组比较,χ2=36.02,P<0.01,差异有极显著性意义.提示复方阿米卡星凝胶能有效防止UTIC的发生;该凝胶取材容易,价格低廉,护理操作简便.