小儿血管环外科治疗的单中心临床分析北大核心CSCD

目的探讨儿童血管环的最佳解剖分型、手术时机、手术方法和治疗效果。方法回顾性分析阜外医院小儿心脏外科中心2014~2019年各类血管环手术治疗58例患儿的临床资料,其中男32例(55.2%)、女26例(44.8%),中位年龄16.5(2~73)个月。对术前症状、影像学检查、血管环分类、手术方法、术后恢复等进行总结分析。结果全组患儿双主动脉弓畸形20例(34.5%),右位主动脉弓伴左侧动脉导管或韧带22例(37.9%),左位主动脉弓伴迷走右锁骨下动脉15例(25.9%),回旋主动脉伴颈位主动脉弓1例(1.7%)。中位呼吸机辅助时间6.0(0~648)h,中位住院时间14.5(7~104)d。全组共1例合并主动脉缩窄患儿围术期因重症肺部感染死亡,其余患儿出院后无症状生存,无再次手术,中位随访时间7.0(1~62)个月。结论对于原发病无法解释的呼吸困难及吞咽困难,或存在右位主动脉弓的患儿,需要术前完善CT或磁共振成像等影像学检查,以明确血管环诊断。手术治疗血管环安全可靠,能有效缓解症状,手术成功率高,再次手术干预率低,随访效果满意。     

Zernike矩在医学图像处理中的应用

矩技术是描述图像特征的一种有效方法,在图像处理的各个领域得到了广泛应用.Zernike矩是一种连续正交矩,可以描述图像形状的全局特征,其最重要的性质是具有旋转不变性.Zernike矩能够构造任意的高阶矩,较容易地从矩特征中对图像进行重构,具有最小的信息冗余,并且对噪声不敏感、鲁棒性好.目前,在医学图像处理中Zernike矩较多应用于边缘检测和图像配准领域.Zernike矩可以达到亚像素级的边缘定位,且抗干扰性强;基于Zernike矩的配准代价函数降低了需要对比的自由度对数,从而显著减少了迭代次数;利用Zernike矩作为图像特征点描述子的算法,在图像存在比例缩放、旋转等情况下具有良好的配准效果.     

hSav1蛋白高表达对Mst1介导的细胞凋亡的影响

Objective To elucidate the effect of hSavl expression on Mstl-mediated apoptosis in HeLa cells. Methods Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and cotransfected into HeLa cells. Triple immunofluorescent labeling of hSav1, Mst1 and nucleus was performed to determine their subcellular localization. Plasmids pCMV-HA-hSav1 and/or pcDNA/4TO-Flag-Mst1 were transfected into HeLa cells, and 36 hours later cisplatin (50 μmol/L) as a pro-apoptotic agent was added for 14 hours. Cell apoptosis was analyzed by annexin V/PI assay. Results Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and the authenticity of constructs was verified by sequencing. The binding in vitro showed that hSavl could be detect from the anti-Mstl immunoprecipitation complex. The immunofluorescent labeling showed that hSavl and Mstl had the same localization in cells. Overexpressed protein hSavl did not induce a significant cell apoptosis. However, co-expression of hSavl with Mstl resulted in a significant increase of apoptosis above the level seen with Mstl alone (24. 5% ± 2.4% vs. 39.3% ± 4.0%, P < 0. 05). Conclusion Our findings indicate that hSavl is a newly identified protein that interacts with Mst1 and a, augments Mst1-mediated apoptosis.     

围术期应用氨溴索对胸腔镜下肺叶切除术后肺癌患者的作用

目的：探讨围术期静脉给予大剂量盐酸氨溴索注射液对胸腔镜下肺叶切除术后肺癌患者的肺功能、术后 并发症、术后住院时间和总住院费用的影响。方法：采用随机对照试验设计,选取中南大学湘雅医院心胸外科2011 年5月至2012年5月行胸腔镜下肺叶切除术的肺癌患者60例分为两组：氨溴素处理组(n=30)和空白对照组(n=30)。氨 溴素处理组自手术当日至术后3 d静脉给予盐酸氨溴索注射液1 000 mg/d;空白对照组则在相应的时间段静脉给予 相同体积的生理盐水。采集并比较两组术前一般资料、术中情况、手术前后的肺功能和血气分析结果、术后早期 并发症、机械通气时间、ICU时间、术后住院时间和总住院费用。结果：两组术前一般资料、术中情况具有可比 性。氨溴素处理组和空白对照组在手术后,第1秒用力呼气容积占预计值百分比(the percent predicted forced expiratory volume in 1 second,FEV1%)、第1秒用力呼气容积占用力肺活量百分比(the ratio of forced expiratory volume in 1 second to forced vital capacity,FEV1/FVC%)、一氧化碳弥散量占预计值百分比(the percent predicted diusing capacity of the lung for carbon monoxide,DLCO%)、动脉血氧分压(PaO2)比手术前均有下降;而空白对照组比氨溴素处理组的下降更明显 (P<0.05)。与空白对照组相比,氨溴素处理组肺部并发症明显减少;术后机械通气时间、ICU时间和术后住院时间明 显缩短;总住院费用显著降低(P<0.05)。结论：围术期静脉给予接受胸腔镜下肺叶切除术的肺癌患者大剂量盐酸氨溴 索注射液,可以改善患者术后肺功能,减少肺部并发症的发生,缩短术后住院时间,降低总住院费用。     

hSav1蛋白高表达对Mst1介导的细胞凋亡的影响

Objective To elucidate the effect of hSavl expression on Mstl-mediated apoptosis in HeLa cells. Methods Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and cotransfected into HeLa cells. Triple immunofluorescent labeling of hSav1, Mst1 and nucleus was performed to determine their subcellular localization. Plasmids pCMV-HA-hSav1 and/or pcDNA/4TO-Flag-Mst1 were transfected into HeLa cells, and 36 hours later cisplatin (50 μmol/L) as a pro-apoptotic agent was added for 14 hours. Cell apoptosis was analyzed by annexin V/PI assay. Results Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and the authenticity of constructs was verified by sequencing. The binding in vitro showed that hSavl could be detect from the anti-Mstl immunoprecipitation complex. The immunofluorescent labeling showed that hSavl and Mstl had the same localization in cells. Overexpressed protein hSavl did not induce a significant cell apoptosis. However, co-expression of hSavl with Mstl resulted in a significant increase of apoptosis above the level seen with Mstl alone (24. 5% ± 2.4% vs. 39.3% ± 4.0%, P < 0. 05). Conclusion Our findings indicate that hSavl is a newly identified protein that interacts with Mst1 and a, augments Mst1-mediated apoptosis.     

基于蒙特卡罗仿真模拟的方舱CT检查患者辐射剂量研究

目的：基于蒙特卡罗仿真模拟方法,研究方舱机房环境下的CT检查患者剂量。方法：采用蒙特卡罗方法和MCNP程序模拟联影uCT530机型,模拟头部模体和体部模体的CT剂量指数(CTDI)测量。对比分析CT扫描中相同管电压、管电流和准直宽度条件下模拟获取的常规机房CTDI值与产品技术手册中CTDI值的差异性;并分别对相同扫描条件下的常规机房与方舱机房的头部模体和体部模体的CTDI值进行对比分析。结果：常规机房内体部模体和头部模体的CTDI值模拟结果与产品技术手册中相应CTDI值比较,差异均无统计学意义(t=-1.39,t=-2.814;P>0.01)。常规机房与方舱机房之间,准直宽度分别为11 mm和22 mm条件下体部模体和头部模体CTDI值模拟结果比较,差异均无统计学意义(t_体部模体=3.909,t=9.54;t_头部模体=3.796,t=3.958;P>0.01)。结论：基于蒙特卡罗仿真模拟方法用于方舱CT检查患者剂量研究结果可靠。尽管方舱CT机房的面积和最小单边长度均小于常规机房的标准要求,但其不会显著增加CT检查患者的辐射剂量。     

二尖瓣成形技术治疗儿童Barlow病的早期疗效

目的 总结二尖瓣成形技术治疗Barlow病合并二尖瓣中度以上反流患儿的临床疗效.方法 回顾性分析2014年1月至2019年8月阜外医院10例Barlow病合并二尖瓣中度以上反流≤ 18岁患儿的临床资料,其中男3例、女7例,平均年龄(8.7±7.9)岁.术前超声心动图和术中瓣膜分析均证实二尖瓣瓣叶冗长、甩动,瓣叶及对合缘...     

hSav1蛋白高表达对Mst1介导的细胞凋亡的影响

目的 观察hSav1蛋白高表达对Mst1介导的细胞凋亡的影响,探讨hSav1在Mst1介导的细胞凋亡中的作用.方法 构建蛋白hSav1的载体pCMV-HA-hSav1与蛋白Mst1的载体pcDNA/4TO-Flag-Mst1,共转染HeLa细胞.采用相应抗体做二者的免疫共沉淀,以证实蛋白hSav1和Mst1之间的相互作用;应用细胞免疫荧光共定位,进一步证实二者之间的相互作用.在HeLa细胞中,分别单独或同时转染质粒pcDNA/4TO-Flag-Mstl和pCMV-HA-hSav1,转染36 h后,加入凋亡诱导剂顺铂(50 μmol/L)作用14 h.应用磷脂结合蛋白碘化丙啶(Annexin V/PI)法检测细胞凋亡,观察蛋白hSav1高表达对Mst1介导的细胞凋亡的影响.结果 载体pCMV-HA-hSav1与pcDNA/4TO-Flag-Mst1构建成功,测序结果表明无突变或缺失.免疫共沉淀结果显示,蛋白hSav1可以在Mst1抗体的免疫沉淀物中检测出来,蛋白Mst1可以在hSav1抗体的免疫沉淀物中检测出来.细胞免疫荧光的结果显示,二者的荧光在细胞内存在共定位,并可充分融合.HeLa细胞中,单独Mst1蛋白高表达组的细胞凋亡率为24.5%±2.4%,单独hSav1蛋白高表达组与对照组比较,无明显细胞凋亡.蛋白Mst1和hSav1高表达组的细胞凋亡率为39.3%±4.0%,差异有统计学意义(P<0.05).结论 蛋白hSav1与Mst1在HeLa细胞内存在相互作用,蛋白hSav1高表达可明显促进由蛋白Mst1介导的细胞凋亡.     

hSav1蛋白高表达对Mst1介导的细胞凋亡的影响

Objective To elucidate the effect of hSavl expression on Mstl-mediated apoptosis in HeLa cells. Methods Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and cotransfected into HeLa cells. Triple immunofluorescent labeling of hSav1, Mst1 and nucleus was performed to determine their subcellular localization. Plasmids pCMV-HA-hSav1 and/or pcDNA/4TO-Flag-Mst1 were transfected into HeLa cells, and 36 hours later cisplatin (50 μmol/L) as a pro-apoptotic agent was added for 14 hours. Cell apoptosis was analyzed by annexin V/PI assay. Results Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and the authenticity of constructs was verified by sequencing. The binding in vitro showed that hSavl could be detect from the anti-Mstl immunoprecipitation complex. The immunofluorescent labeling showed that hSavl and Mstl had the same localization in cells. Overexpressed protein hSavl did not induce a significant cell apoptosis. However, co-expression of hSavl with Mstl resulted in a significant increase of apoptosis above the level seen with Mstl alone (24. 5% ± 2.4% vs. 39.3% ± 4.0%, P < 0. 05). Conclusion Our findings indicate that hSavl is a newly identified protein that interacts with Mst1 and a, augments Mst1-mediated apoptosis.     

儿童胆囊扭转超声表现1例

患儿男,8岁.主因"间断右上腹疼痛、呕吐1个月"来诊.曾在外院按"胆囊炎"治疗效果不佳.查体:右上腹压痛,余未见异常.超声检查:胆囊约3.1cm×1.1cm大小,尚清晰;右上腹可见一约4.5cm×3.6 cm×2.6 cm大小混合回声肿块,壁非对称性增厚,与胆囊底毗邻;整个胆囊呈"8"字形"葫芦状",其"8"字交叉部位为带状高回声(图1).