Meek植皮修复大面积深度烧伤创面

目的探讨Meek植皮法修复大面积深度烧伤创面的临床疗效。方法采用Meek植皮法修复36例大面积深度烧伤患者创面,与同期采用小邮票植皮法修复的33例大面积深度烧伤患者进行对比观察,内容包括移植皮片成活率、皮片融合时间、植皮手术操作时间、皮片扩张率等。结果 Meek植皮组的移植皮片成活率为（97.6±2.1）%,皮片融合时间为（11.2±1.0）d,植皮手术操作时间为（1.1±0.1）min/1%,皮片扩展率为（4.3±0.4）%,小邮票植皮组的移植皮片成活率为（96.5±2.0）%,皮片融合时间为（14.2±1.4）d,植皮手术操作时间为（4.5±0.9）min/1%,皮片扩展率为（2.7±0.5）%,两组比较,移植皮片成活率差异无统计学意义,皮片融合时间、皮片扩展率差异有统计学意义（P〈0.05）,植皮手术操作时间差异有统计学意义（P〈0.01）。结论 Meek植皮具有简化手术操作,缩短植皮手术时间,缩短皮片融合时间,节约皮源以及移植皮片成活率高等优点,适合于大面积深度烧伤创面的修复。     

Expression of NADPH oxidase and production of reactive oxygen species in aorta in an active immunization mouse model with AT1-EC2 peptide

The antibody against AT1-EC2 plays a role in some kinds of inflammatory vascular diseases including malignant hypertension,preeclampsia,and renal-allograft rejection,but the detailed mechanisms remain unclear.In order to investigate the changes of NADPH oxidase and reactive oxygen species in the aorta in a mouse model which can produce AT1-EC2 antibody by active immunization with AT1-EC2 peptide,15 mice were divided into three groups:control group,AT1-EC2-immunized group,and AT1-EC2-immunized and valsartan-treated group.In AT1-EC2-immunized group and AT1-EC2-immunized and valsartan-treated group,the mice were immunized by 50 μg peptide subcutaneously at multiple points for 4 times:0,5,10,and 15 days after the experiment.In AT1-EC2-immunized and valsartan-treated group,valsartan was given at a dose of 100 mg/kg every day for 20 days.After the experiment,the mice were sacrificed under anesthesia and the aortas were obtained and frozen in liquid nitrogen for the preparation of frozen section slides and other experiments.The titer of AT1-EC2 was assayed by using ELISA.The level of NOX1 mRNA in the aorta was determined by using RT-PCR.The expression of NOX1 was detected by using Western blotting.Confocal scanning microscopy was used to assay the α-actin and NOX1 expression in the aortic tissue.The O 2.production was detected in situ after DHE staining.The mice produced high level antibody against AT1-EC2 in AT1-EC2-immunized group and AT1-EC2-immunized and valsartan-treated group,and the level of NOX1 mRNA in the aortic tissues was 1.6±0.4 times higher and the NOX1 protein expression was higher in AT1-EC2-immunized group than in control group.There were no significant differences in the level of NOX1 mRNA and protein expression between control group and AT1-EC2-immunized and valsartan-treated group.The expression and co-localization of α-actin and NOX1 in AT1-EC2-immunized group increased significantly as compared with those in control group,and the O 2.production increased about 2.7 times as compared with contro     

急性冠脉综合征患者血清胱抑素C水平与支架置入术后不良血管事件的相关性研究

目的 研究急性冠脉综合征(ACS)患者血清胱抑素C(Cystatin C,Cys-C)水平与冠脉内支架置入术后主要不良心血管事件(MACE)发生的关系.方法 回顾性分析156例冠脉内支架置入术治疗的ACS患者资料,将Cys-C水平按四分位法分为4组,比较各组术后6月内MACE发生率;按有无MACE分组,比较两组间血清Cys-C水平差异,Logistic回归分析筛选MACE危险因素.结果 血清Cys-C水平,最高组与最低组相比MACE发生率明显增高(29.3％ vs 10.2％,P＜0.01),MACE组与无MACE组相比明显增高[(1.37±0.15)mg/L,(1.11±0.18)mg/L,(P＜0.01)];Logistic回归分析表明,血清Cys-C水平是导致MACE发生的危险因素(OR 2.248,P＜0.001).结论 ACS患者血清Cys-C水平与冠脉内支架置入术后短期MACE发生率呈正相关,且是术后短期MACE的危险因素.     

体外循环术后高血糖的研究现状

高血糖是心脏大血管术后常见的不良因子,目前研究可知机体内外许多因素参与了其发生过程。大量研究显示体外循环术后高血糖与术后患者心、脑、肺等多脏器并发症的发生以及不良预后存在密切关系。及时监测血糖水平并合理应用胰岛素对其进行有效控制可明显降低术后并发症的发生率及围术期患者病死率,对术后患者的预后具有重要意义。     

高校图书馆专业技术人员考核的实践与思考

结合右江民族医学院图书馆对专业技术人员考核的情况,从考核目的、考核标准、考核方法、考核计分、考核周期等方面对高校图书馆考核进行了探讨.     

牙科用锆基非晶合金的细胞毒性及血液相容性研究

目的:测定高强度、高弹性模量的新型口腔种植用锆基非晶合金的细胞毒性及血液相容性。方法:通过锆基非晶合金与新鲜人血接触进行血液相容性实验并采用MTT法研究锆基非晶合金的细胞毒性。结果:细胞毒性试验显示本研究制备的锆基非晶合金的细胞毒性反应分级为0级,无细胞毒性;血液相容性试验结果锆基非晶合金溶血率低,动态凝血时间较纯钛及Ti₆Al₄V延长,以PT、APTT为指标的抗凝血性能与纯钛、Ti₆Al₄V相当,血小板粘附试验中Zr-Cu-Al-Ag较少出现血小板黏附,团聚及伪足伸出现象。结论:Zr-Cu-Al-Ag拥有良好的细胞相容性、血液相容性,可以初步满足其作为口腔种植材料的基本应用要求。     

2007～2008年南京市鼓楼区医疗机构消毒质量监测

[目的]为了解鼓楼区医疗机构消毒质量状况,找出消毒中存在问题,控制医院感染。[方法]依据卫生部《消毒技术规范》(2002年版)和《医院消毒卫生标准》(GB15982-1995),于2007～2008年,每年5～10月对南京市鼓楼区医疗机构消毒质量检测资料进行分析。[结果]2007～2008年合计检测样品1800份,合格的1524份,合格率为84.67%。2008年合格率高于2007年,差异无统计学意义(P0.05)。辖区医院为95.14%(352/370),乡镇卫生院为87.18%(639/733),个体诊所为76.47%(533/697)。三者间的差异有统计学意义(P0.01)。[结论]医疗机构应加强对消毒工作管理,强化消毒人员的业务培训和制度落实。有关部门应加强对医疗机构消毒管理的监督工作。     

卡维地洛治疗慢性心力衰竭疗效预测参数研究

目的运用常用的临床指标预测卡维地洛（Carvedilol）治疗慢性心力衰竭（CHF）的效果。方法44例心衰患者（NYHAⅡ～Ⅳ）随机分为常规治疗组和卡维地洛组,并且根据治疗效果再将卡维地洛组分为疗效显著亚组（A组）和疗效不显著亚组（B组）,通过临床监测探讨卡维地洛疗效显著的预测参数。结果（1）卡维地洛治疗可以明显改善患者心功能、抑制心肌重构;（2）A组患者（n=13）收缩压明显高于B组（n=7）（P〈0．05）;（3）A组血清Na^＋浓度高于B组、血清K^＋低于B组（P〈0．05）。结论从卡维地洛治疗心衰疗效显著的患者分析中,提示治疗前的收缩压水平、血清Na^＋和血清K^＋浓度可以预测CHF的疗效。