补肾健骨类中药活性成分对高糖诱导成骨细胞的保护作用及机制研究

目的:探讨柚皮苷、补骨脂二氢黄酮、淫羊藿苷等3种补肾健骨类中药活性成分对高糖诱导成骨细胞的保护作用和机制.方法:建立高糖诱导成骨细胞模型;实验设正常对照组、模型组(30mmol/L葡萄糖)和给药组.给药组为分别在模型细胞中加入补骨脂二氢黄酮组、柚皮苷组和淫羊藿苷.作用48h后,观察细胞形态,MTT法检测成骨细胞活性,R...     

化痰行瘀法治疗肝纤维化

中医认为痰瘀互结是肝纤维化(hepaticfibrosis,HF)的病机关键,治疗应化痰行瘀法为主。结合临床体会论述了肝纤维化的中医发病机制和治法方药,为中医药治疗肝纤维化从化痰行瘀法来研究提供理论依据。     

颈总动脉结扎联合半乳糖注射制备老年期痴呆大鼠模型及行为学评价研究简

目的 探讨不同制备方法对痴呆大鼠模型学习记忆能力的影响,为药效评价提供老年期痴呆大鼠模型.方法 40只Wistar雄性大鼠随机分为5组：分期双侧颈总动脉永久结扎后D-半乳糖注射组（A组）;D-半乳糖注射后分期双侧颈总动脉永久结扎组（B组）;单次双侧颈总动脉永久结扎组（C组）;D-半乳糖注射组（D组）,正常对照组（E组）,每组8只.除E组外,A组左侧颈总动脉结扎,间隔1周后行右侧颈总动脉结扎术;并于结扎术1周后行D-半乳糖腹腔注射（60 mg/kg体重,每天1次）,共连续注射42 d;B组腹腔连续注射D-半乳糖（60 mg/kg体重）42 d后,再行双侧颈总动脉永久结扎术;C组仅行双侧颈总动脉永久结扎术42 d,腹腔不注射半乳糖;D组：仅连续腹腔注射D-半乳糖（60 mg/kg体重）42 d,不接扎其双侧颈总动脉.最后采用Morris水迷宫行为学测试法进行定位航行及空间探索实验;取各组大鼠海马CA1区进行电镜观察.结果 与C、E组相比,A、B及D组游泳时间与距离均明显缩短,差异有统计学意义（P＜0.01）;而A组与B、D组之间差异无统计学意义（P＞0.05）;比较正常组各模型组海马CA1区锥体细胞有不同程度的丢失,且脑组织病理学改变明显.结论 采用双侧颈总动脉永久结扎联合半乳糖腹腔注射方法,制备的老年期痴呆大鼠模型,记忆力明显减退,且脑组织病理学显著改变,能有效重现老年期痴呆患者的发病特点.     

苦参碱对海马脑片电信号及氨基酸神经递质的作用

BACKGROUND： Studies on electrical signals of hippocampal brain slices in vivo have shown that matrine inhibits benzylpenicillin sodium-induced activation of neuronal signal transduction.
OBJECTIVE： To verify the inhibition effect of matrine on activation of electrical signals in rat brain slices and the role rnatrine plays in hippocampal amino acid transmitter release.
DESIGN, TIME AND SETTING： The in vitro, neurophysiological, controlled experiment was performed in the Zhejiang Province Key Laboratory of Cardio-cerebrovascular Disease and Nerve System Drugs Appraisement and Chinese Traditional Medicine Screening and Research between July 2003 and May 2004. The in vivo, neuronal, biochemical experiment was performed in the Zhejiang Province Key Laboratory of Chinese Traditional Medicine Quality Standardization from July 2005 to December 2006.
MATERIALS： Forty healthy, Sprague Dawley rats, 7-8 weeks old, and 120 healthy, ICR mice, 5-6 weeks old, were included in this study, irrespective of gender. Matrine powder was provided by the National Institute for the Control of Pharmaceutical and Biological Products, China. Matrine injection was purchased from Zhuhai Biochemical Pharmaceutical Factory, China. Penicillin was bought from Shijiazhuang Pharmaceutical Group Co., Ltd., China.
METHODS： （1） Rats were randomly assigned to four groups： control, penicillin model, and matrine high-dose and low-dose, with 10 rats in each group. The control group was perfused with artificial cerebrospinal fluid. In the remaining three groups, hippocampal brain slices were perfused with normal artificial cerebrospinal fluid containing 1 × 106 U/L penicillin for the first 10 minutes. The penicillin model group received artificial cerebrospinal fluid for an additional 30 minutes, while the matrine high-dose and low-dose groups received 0.1 g/L and 0.05 g/L matrine, respectively, for an additional 30 minutes. （2） Mice were randomly assigned to four groups （n = 30）. The matrine high-, medium-, and low-dose groups were separately injected with 58.5, 39.0, and 19.5 mg/kg matrine via caudal vein, respectively. No intervention was administered to the normal group.
MAIN OUTCOME MEASURES： The field potential value in the CA1 region of penicillin-induced rat hippocampal brain slices was analyzed using the evoked field potential technique; chromatography was utilized to determine y-aminobutyric acid and glutamic acid content in the mouse hippocampus.
RESULTS： （1） Both 0.1 g/L and 0.05 g/L matrine reduced the number of evoked field potentials in the penicillin-induced rat hippocampal brain slices （P 〈 0.05 or P 〈 0.01 ）. In addition, 0.1 g/L matrine led to a reduction of evoked field potential amplitude （P 〈 0.05）. （2） Compared with normal mice, γ-aminobutyric levels were dramatically increased at 20 minutes after high-dose matrine treatment （P 〈 0.05）. In addition, significantly increased γ-aminobutyric acid levels were observed at 40 minutes after medium- and low-dose matrine treatments （P 〈 0.05 or P 〈 0.01 ）. The glutamic acid/γ-aminobutyric acid ratio was significantly less at 20 minutes after high-dose matrine treatment compared with the normal mice group （P 〈 0.05）.
CONCLUSION： Matrine exerts a central inhibitory effect via increased inhibitory neurotransmitter γ-aminobutyric acid levels in the hippocampus.     

苦参碱注射液对小鼠脑海马氨基酸神经递质的作用研究

苦参碱（MAtrine）是豆科槐属植物苦参（Sophora flavescnes Ait）、苦豆子（S.Alopecuroides L）、广豆根（S、subprostrata Chunet T、Chen）等中草药的活性成分。作为苦参碱类生物碱的代表，它具有多种药理活性，注射制剂已被广泛用于临床治疗慢性肝炎。近年来国内外在研究苦参碱的过程中，进一步发现了其多方面的药理作用，具有很大的再开发价值。本文在中药苦参醇提取液镇静催眠药理学实验基础上，进行了苦参碱注射液对小鼠脑海马内氨基酸神经递质的作用研究。     

白术内酯的药理学研究进展

白术为菊科植物白术Atractylodes macrocephala Koidz的干燥根,主产于浙江、安徽、湖北、湖南等省。其性味甘、苦,温,具有健脾益气、燥湿利水、止汗、安胎的功效。其主要含有挥发油成分和内酯类成分,其中内酯类成分具有抗炎、抗肿瘤作用,并具有调节胃肠道功能和促进营养物质吸收的功效,尤以白术内酯Ⅰ作用显著。近年来,许多学者对白术进行了化学、药理和炮制等方面的研究。本文就白术主要化学成分——白术内酯的药理作用研究进展作如下综述。     

苦参碱对新生大鼠海马神经细胞的影响

苦参碱(Matrine)是豆科槐属植物苦参、苦豆子、广豆根等中草药的活性成分,具有广泛的生理活性[1].本文在前期研究工作的基础上,进行苦参碱对原代大鼠海马神经细胞形态观察,用MTT比色法比较不同浓度苦参碱对神经细胞生存率的影响,以期为研究苦参碱对中枢神经作用提供科学依据. 1 材料与试剂 1.1 试验动物:为新生大鼠,由浙江省中医药研究院动物房提供,浙动字医第22-9601009号.     

补肾强骨胶囊抗骨质疏松药效学的研究

目的 :研究补肾强骨胶囊的抗骨质疏松作用。方法 :利用空白和阳性对照 ,观察补肾强骨胶囊的药理作用 ,并对数据进行生物学统计。结果 :补肾强骨胶囊 ,可使“肾虚”模型小鼠的睾丸重指数及子宫重指数明显增加 ,可提高去势大鼠的股骨密度 ,能减少维甲酸性骨质疏松模型大鼠股骨骨折数 ,显著增加股骨重量 ,提高血钙浓度 ,还具有镇痛、抗炎作用。结论 :补肾强骨胶囊有良好的抗骨质疏松作用 ,为临床疗效提供了实验依据。