生脉注射液对大鼠心肌缺血的保护作用

大鼠腹腔注射生脉注射液可降低垂体后叶素诱发冠脉痉挛所致的急性心肌缺血阳性率，并对异丙肾上腺素引起的心肌缺血缺氧具有拮抗作用。实验结果表明，本药具有抗心肌缺血的作用。     

心肺联合移植术后管理和并发症的处理

目的总结两例心肺联合移植术（CHLT）后并发症处理的经验。方法对两例先心病室间隔缺损艾森曼格综合征CHLT管理和术后出现的出血、肺部感染、肺不张、肺或心脏的急性排斥反应等并发症进行综合处理，包括积极输血和纠正凝血紊乱、抗感染、肺不张复张术、免疫冲击治疗以及免疫方案的调整、呼吸机辅助呼吸、营养支持等。结果术后多种并发症已基本控制；两位患者均存活（分别为632天和508天）。结论CHLT后严密监测和及时有效的处理并发症对减少死亡率和改善预后有重要的意义。     

如何确保医学检验质量的可靠性

医学检验是一门高度综合性的应用科学,随着医疗技术的迅猛发展,医学检验在医疗活动中的地位越来越受到人们的重视。如果忽视了医学检验质量,就无法保障医疗、科研、保健等医技科室的正常活动。现根据多年的临床检验实践,就如何确保医学检验质量的可靠性谈几点观点:1重视人才培养是提高医学检验质量的根本卫生技术人员的培养是卫生事业进步的关键,而培养一批具有高层次文化素质的检验工作者,既是保证医学检验的基础,又是反映医院业务和学术水平必不可少的一个标志。检验科除了准确及时提供检验信息外,还要进行诊断、病情分析、参加抗菌药物耐…     

类固醇5-α还原酶亚型与良性前列腺增生的研究进展

类固醇 5 α还原酶通过将睾酮转变为双氢睾酮 ,在人类前列腺的发生、成熟及良性前列腺增生的形成演变过程和治疗药物的研制开发中具有重要作用 ,本文就该酶亚型与良性前列腺增生的相关研究进展进行综述。     

哮喘患者血浆ECP水平的临床观察

目的 :探讨血浆嗜酸性粒细胞阳离子蛋白 (ECP)在哮喘诊断及治疗中的价值。方法 :应用荧光酶免法定量测定 4 2例健康体检者、30例急性期及缓解期哮喘患者血浆ECP含量。结果 :急性组、缓解组与正常组比较 ,血浆ECP水平升高 (P <0 .0 1 ,P <0 .0 5 ) ;急性组与缓解组血浆ECP水平亦有显著性差异 (P <0 .0 1 )。结论 :血浆ECP水平检测可作为哮喘患者诊断、监测、预后及指导抗炎治疗的参考指标。     

Effect of schisandrin B on lung mRNA expression of transforming growth factor-β1 signal transduction molecule in rat lungs exposed to silica

Objective To investigate the effects of schisandrin B (Sch-B) on expression of transforming growth factor-β1 (TGF-β1) and signal transduction molecule mRNA in rat lungs exposed to SiO2,and explore the intervention mechanism of Sch-B on pulmonary fibrosis induced by SiO2. Methods Ninety six Wistar rats were randomly divided into control (normal saline) group, SiO2 group and SiO2 plus Sch-B group.The rats were exposed to SiO2 by direct tracheal instillation to establish the silicotic animal models. SiO2 group and SiO2 plus Sch-B group were treated with 1ml SiO2 (50 mg/ml) for each rat From the first day after model establishment, SiO2 plus Sch-B group were orally given Sch-B (80 mg/kg) a day, control group and silica group were orally given olive oil. On the 3rd, 7th,14th and 28th days after treatment, 8 rats in each group were sacrificed and samples were collected. The histo-pathological examination of lung was performed by HE staining. The expression levels of TGF-β1 、TGFβR Ⅱ and Smad4 mRNA in the lung tissues were detected by RT-PCR. Results The results of histo-pathological examination showed that in SiO2 group, lung tissues were injured obviously; the alveolar inflammation with alveolus interval edema and inflammation cell infiltration appeared on the 3rd and 7th days; the alveolus interval became thicker, became thicker, fibroblast and collagen matiix increased markedly on 14th day; the alveolar structure was damaged, alveolar wall thickened obviously,collagen aggradation and pulmonary fibrosis displayed on 28th day. The alveolar inflammation and pulmonary fibrosis in SiO2 plus Sch-B group were significantly less than those in SiO2 group. The expressions levels of TGF-β1、TGFβR Ⅱ and Smad4 mRNA (TGF-β1: 1.03±0.31 、1.33±0.39、1.08±0.26、0.82±0.16,TGF-βR Ⅱ:0.65 ±0.11、0.80 ±0.16、0.83 ±0.24、0.62 ±0.15, Smad4: 0.87 ±0. 15、0.68 ±0.11、0.78 ±0.19、0.30 ±0.08) in SiO2group were significantly higher than those in the control group (TGF-β1:0.59±0.22、0.55 ±0.25、0.56±0.20、0.55 ±0.12,TGR-βR Ⅱ:0.28 ±0.13、0.31 ±0. 15、0.34 ±0.15、0.27 ±0.09,Smad4:0.23 ±0.11、0.40 ±0. 12、0.39 ±0.12、0.18±0.06)(P＜0.01 or P＜0.05), but the expression level of TGF-β1 mRNA was the highest on the 7th day. The expression levels of TGF-β1 and Smad4 mRNA (TGF-β1: 0.68 ±0.28、0.88 ±0.25、0.75 ±0.11、0.61 ±0. 14,Smad4:0.25 ±0.12、0.45 ±0.09、0.44 ±0.07、0.21 ±0.04) in SiO2 plus Sch-B group were significantly lower than those in SiO2 group (P＜0.01 or P＜0.05),but there were no significant differences of the TGFβR Ⅱ mRNA expression levels between SiO2 group and SiO2 plus Sch-B group. Conclusion Sch-B can reduce the pulmonary fibrosis induced by SiO2 through inhibition of the mRNA express of TGF-β1 and Smad4 in the lung tissue, modulating the TGF-β1/Smad4 signal transduction pathway and inhibiting the target gene activation.     

中药红昇丁治疗口腔脓性骨髓炎

自从外科金伯恭先生介紹使用紅昇条及紅昇丁治疗骨結核有疗效显著后,我科(口腔科)曾使用于口腔下頜骨髓炎,亦得良好效果.过去遇到这类疾病一貫采用开刀,刮除病灶及死骨。如手术不彻底,术后往往有化脓成瘘的麻煩。今用中医的办法,既縮短疗程,又減少病人痛苦。茲將兩例典型例子向同道介紹。一、药的来源 紅昇丁及紅昇条均为中医外科成药,患部二寸以內的插丁,二寸以外的插条。二、治疗方法 1.上药前先以探針探明瘻管的長短方向,如管深而頑固的,須先以槽针翻入少許紅昇丹到瘡口底部。以加强药力。 2.换药前先用百分之三双氧水二十西西注入注射器,再套一尼龙管塞入創口,二十西西分四次冲洗,每洗一次,停兩三分鐘再洗,使管內死骨或腐性分泌     

“加碘试验”诊断早期妊娠100例观察

笔者在1988年阅读一本杂志时,曾看到其上刊载了题为“简单的实验”一文后,就将其法应用于临床,通过2年多的时间,对其观察,笔者认为此方法简单易行,准确性可以同尿妊娠试验的胶乳凝集抑制试验相比,有一定的实用价值,值得推广。 1.一般资料在此100例观察人次中,停经2个月以下的43例,停经2～3个月的49例,停经     

丝裂霉素或顺铂对离体淋巴因子激活的杀伤细胞增殖和抗膀胱癌细 …

AIM: To study the effect of mitomycin (Mit) or cisplatin (Cis) on the proliferation of lymphokine-activated killer (LAK) cells in patients with transitional cell cancer of bladder and their cytolysis to bladder tumor cells. METHODS: LAK cell proliferation was assayed in the presence of Mit or Cis by cell counting. Bladder cancer cell lines BIU-87 and EJ were cultured as target cells and cytotoxicity of LAK cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The proliferation of LAK cells induced by recombinant interleukin-2 (IL-2) was inhibited by Cis in a concentration-dependent manner and was decreased to 55.3% at 100 mg.L-1 compared with control at 96 h. The enhanced growth of the LAK cells was observed with Mit 5-10 mg.L-1 from 48 to 96 h. Cis 10 mg.L-1 increased the cytotoxicity against BIU-87 and EJ cells. CONCLUSION: Immunomodulatory effect of chemotherapeutic agents on LAK cell proliferation induced by IL-2 in patients with bladder cancer mainly depends on the drug itself.     

427例血栓闭塞性脉管炎分期辨治报告

对确诊为血栓闭塞性脉管炎的４２７例患者，按照临床分为３期进行辩证论治，Ⅰ期治以温经散寒，活血通络；Ⅱ期治以活血化瘀通络；Ⅲ期治以清热凉血，活血解毒。治疗结果：临床治愈１７０例，显效１５５例，好转９６例，无效６例。