浅谈案例教学法在保险法教学中的应用

保险法教学应当紧密联系立法与司法实践，以培养学生分析问题和解决问题的能力.实现这一目标,在沿用传统的保险法教学方法的基础上,各高等院校广泛引入了案例教学法.案例教学法越来越受到关注,已成为众多教学方法改革中非常重要的一项.案例教学法的实施,可有效促进保险法教学水平的提高,增强学生的实践能力,这是值得大力推广和倡导的一种教学方法.本文联系保险法教学实际,浅谈案例教学法在保险法教学中的意义及具体应用.     

量子点荧光标记技术与免疫组织化学染色检测泌尿系统肿瘤组织特异性抗原表达的效果比较

目的 通过比较量子点(QDs)荧光标记技术与免疫组织化学(IHC)染色检测膀胱癌组织标本中CD44v6抗原和前列腺癌组织标本中前列腺干细胞抗原(PSCA)的效果,观察该标记技术的敏感性.方法 采用QDs荧光标记技术和IHC染色检测80例膀胱癌组织中的CD44v6抗原和40例前列腺癌组织中PSCA的表达.结合肿瘤临床分期分级,评估两种标记方法得出的肿瘤分级.结果 CD44v6抗原在膀胱癌组织中的表达与肿瘤的分级(QDs:r=-0.345,P=0.002;IHC:r=-0.392,P＜0.001)和分期(QDs:r=-0.243,P=0.03;IHC:r=-0.306,P=0.006)呈负相关,两种方法的Kappa值为0.931 (P＜0.001),说明两种标记方法对膀胱癌组织中CD44v6抗原的检测结果具有一致性.另外,PSCA在前列腺癌组织中的表达与肿瘤的Gleason评分(QDs:r=0.732,P ＜0.001;IHC:r =0.683,P＜0.001)和分期(QDs:r=0.514,P=0.001;IHC:r=0.432,P=0.005)呈正相关,两种方法的Kappa值为0.874(P ＜0.001),说明两种标记方法对前列腺癌组织中PSCA的检测结果具有一致性.结论 QDs荧光标记具有良好的敏感性,该技术在泌尿系肿瘤研究领域具有广阔前景,为肿瘤诊治水平奠定了良好的基础.     

乡镇卫生院信息化建设人才培养需求分析

目的：探讨加快乡镇卫生院信息化建设进程的适宜途径。方法：对安徽省221所中心卫生院、349所一般卫生院卫生信息管理现状及需求情况进行问卷调查;对部分乡镇卫生院院长进行个人访谈。结果：95．51％的中心卫生院、27．5％的一般卫生院设有相对稳定的信息工作人员;未来两年乡镇卫生院对卫生信息类人才在一定程度上还有较大的总量需求,有1／3的采用兼职人员在卫生信息岗位上从事相关工作;乡镇卫生院在计算机应用技能的培训需求最大。结论：推进乡镇卫生院信息化是加快卫生院发展I必由之路,需要上级部门及领导的重视,应加大对信息化人才的引进和培养的力度,多途径、多举措的提高乡镇卫生院信息化水平。     

加快乡镇卫生院信息化建设的策略研究

目的：探讨“十一五”时期安徽省乡镇卫生院信息化建设的基本策略。方法：社会评估和横断面调查相结合。结果：乡镇卫生院已普遍具有信息化意识和硬件基础,但建设进度与农村卫生事业发展要求不相适应;信息化建设中需要得到的首要支持是政府信息化建设专项投入;收费结算处和护理部等管理科室仍为当前信息化建设重点科室。临床业务科室信息化未引起足够重视;提高职工卫生信息管理技能是卫生院长信息化培训主要需求。结论：乡镇卫生院信息化建设资金以机构自筹为主,政府适度引导;重点加强在岗职工信息化培训,逐步形成专兼结合卫生信息队伍;全面推行信息化专项考核,落实信息化建设分级职责。     

慢病毒介导CCL5-siRNA对乳腺癌细胞生物学行为的影响

Objective To study the effect of lentivirus-mediated CCL5-RNAi on the biological behaviors of human breast cancer cells. Methods CCL5-specific siRNA gene was synthesized and cloned into the recombinant lentiviral vector, pGCSIL-GFP. Human high-metastatic breast cancer cells, MDA-MB-231, were infected by CCL5-siRNA recombinant lentivirus, which was set as KD group. Cells infected with CCL5-NC was as NC group, and cells cultured was as CON group. The expression of CCL5 mRNA and protein in MDA-MB-231 cells was detected by RT-PCR and western blot, respectively. Cell growth suppression and cell cycle was observed by MTT assay and fluorescence activated cell sorting (FACS). Colony formation and migration ability were determined by colony-rorming assay and Boyden chamber method. Results After infection of CCL5-siRNA recombinant lentivirus, the expression level of CCL5 mRNA and protein in MDA-MB-231 cells as well as the colony formation and migration ability decreased significantly, but cell's proliferation was not affected obviously. Compared with MDA-MB-231 (0.88± 0.15) and MDA-MB-231/CCL5-NC (1.00±0.07) cells, the expression of CCL5 mRNA in MDA-MB-231/ CCL5-siRNA decreased to 0.18±0.03, P<0.01. Compared with MDA-MB-231/CCL5-NC (1.82±0.18) cells, the expression of CCL5 protein in MDA-MB-231/CCL5-siRNA decreased to 0.33±0.13, P <0.01. Colony-forming assay and Boyden chamber method showed that the colony formation and migration ability of MDA-MB-231/CCL5-siRNA decreased markedly (P<0.05). The clone count in KD group was (0.33± 0.10), which was a significant decrease from (0.97±0.09) (NC group) and (1.04±0.07) (CON group), P<0.05. The number of cells that migrated through the chamber membrane of KD group (38± 15) was less than that of NC group (77±11, P <0.05) and CON group (69±9, P <0.05). However, MTT assay and FACS revealed that the proliferation of MDA-MB-231/CCL5-siRNA was not different from MDA-MB-231/CCL5-NC and MDA-MB-231 (P>0.05), the proliferation index (PI) of group KD, NC and CON were (0.48±0.02), (0.44±0.05) and (0.47±0.02) respectively. The difference was not statistically significant by multiple comparison (P>0.05). Conclusion CCL5-specific siRNA can specifically suppress the colony formation and migration of human high-matastatic breast cancer cells.     

男护生专业认同感现状及思考

自南丁格尔开创现代护理专业以来,护理工作一直是以女性为主导的职业。近些年来随着社会发展和护理专业发展的需要,男护士开始逐渐走上了护理岗位。但是面对这个长期由女性“一统天下”的职业,男护生的专业认同情况如何将直接影响到他们今后的专业学习和职业发展。因此有必要了解男护生的专业认同情况并采取适当的培养策略,以期提升其专业认同度,为护理事业的发展输送更多高质量的人才。     

安徽省乡镇卫生院调查与研究报告

该报告对安徽省1677个乡镇卫生院的基本情况、卫生资源及服务能力等进行了系统调查与研究,提出中心卫生院可根据市场需求向医院方向发展,一般乡镇卫生院实行医疗和防保并重的原则;可鼓励民间资金的投入,促进乡镇医疗卫生机构的改革;“十五”期间,乡镇卫生院建设的财政投入应从硬件投入为主转为以卫生人力教育培训为主等一系列观点与建议。     

慢病毒介导CCL5-siRNA对乳腺癌细胞生物学行为的影响

Objective To study the effect of lentivirus-mediated CCL5-RNAi on the biological behaviors of human breast cancer cells. Methods CCL5-specific siRNA gene was synthesized and cloned into the recombinant lentiviral vector, pGCSIL-GFP. Human high-metastatic breast cancer cells, MDA-MB-231, were infected by CCL5-siRNA recombinant lentivirus, which was set as KD group. Cells infected with CCL5-NC was as NC group, and cells cultured was as CON group. The expression of CCL5 mRNA and protein in MDA-MB-231 cells was detected by RT-PCR and western blot, respectively. Cell growth suppression and cell cycle was observed by MTT assay and fluorescence activated cell sorting (FACS). Colony formation and migration ability were determined by colony-rorming assay and Boyden chamber method. Results After infection of CCL5-siRNA recombinant lentivirus, the expression level of CCL5 mRNA and protein in MDA-MB-231 cells as well as the colony formation and migration ability decreased significantly, but cell's proliferation was not affected obviously. Compared with MDA-MB-231 (0.88± 0.15) and MDA-MB-231/CCL5-NC (1.00±0.07) cells, the expression of CCL5 mRNA in MDA-MB-231/ CCL5-siRNA decreased to 0.18±0.03, P<0.01. Compared with MDA-MB-231/CCL5-NC (1.82±0.18) cells, the expression of CCL5 protein in MDA-MB-231/CCL5-siRNA decreased to 0.33±0.13, P <0.01. Colony-forming assay and Boyden chamber method showed that the colony formation and migration ability of MDA-MB-231/CCL5-siRNA decreased markedly (P<0.05). The clone count in KD group was (0.33± 0.10), which was a significant decrease from (0.97±0.09) (NC group) and (1.04±0.07) (CON group), P<0.05. The number of cells that migrated through the chamber membrane of KD group (38± 15) was less than that of NC group (77±11, P <0.05) and CON group (69±9, P <0.05). However, MTT assay and FACS revealed that the proliferation of MDA-MB-231/CCL5-siRNA was not different from MDA-MB-231/CCL5-NC and MDA-MB-231 (P>0.05), the proliferation index (PI) of group KD, NC and CON were (0.48±0.02), (0.44±0.05) and (0.47±0.02) respectively. The difference was not statistically significant by multiple comparison (P>0.05). Conclusion CCL5-specific siRNA can specifically suppress the colony formation and migration of human high-matastatic breast cancer cells.     

基于人文关怀能力培养的高职高专护理教育模式研究

目的 以护生人文关怀能力培养为关切点,探索建立适应现代社会发展需要的高职高专护理人才培养模式.方法 创建并实施“专业分向”教育模式,将护理专业分为八个专业方向,进行课程体系和教学方法改革,突出素质教育和人文关怀能力培养.结果 培养了全面发展的高素质护理人才,绝大多数学生对“专业分向”教育模式及自己的护理人文关怀能力评价良好,100％的用人单位对毕业生满意,93.3％的用人单位认为毕业生人文关怀能力很强.结论“专业分向”教育模式实施效果良好,可为高职院校教育教学改革提供有益的借鉴.