河南省农村中医适宜技术影响因素研究

目的 了解影响河南省农村中医适宜技术应用的主要因素.方法 采用典型抽样的方法,对108名从事基层医疗工作的医生进行了问卷调查.结果 影响中医适宜技术应用的因素依次为:领导的重视程度(66.7%)、技术的疗效(63.9%)、老百姓的接受程度(56.5%)等.聚类分析后可将影响中医适宜技术应用的14个指标聚为3类,Ⅰ类包含九个指标,反映了实施适宜技术单位方面的因素,Ⅱ类包含四个指标,反映了适宜技术作用客体方面的因素,Ⅲ类一个指标,反映了领导的重视程度.结论 加强领导,重视乡村医生中医技能培训.提高适宜技术的疗效,动员更多的老百姓接受中医适宜技术是推广农村中医适宜技术的关键环节.     

六味地黄丸(大蜜丸)质检中存在问题探讨

六味地黄丸(大蜜丸)是由山茱萸、熟地等六味药材经加工而制成的传统中药制剂.用于肾阴亏损、头晕耳鸣、腰膝酸软、骨蒸潮热等[1].由于近年山茱萸的价格不断上涨,致使六味地黄丸的价格也随之上升.可是,从2000年抽验的情况发现,有些厂家六味地黄丸的零售价都以低于该药的成本价进行销售.因此我所对该批药产生怀疑,并对其进行抽样检验.     

早期足量复能剂抢救中、重度有机磷农药中毒临床研究

观察早期足量复能剂配台适量阿托品救治急性有机磷农药中毒（AOPP）的疗效。     

幽门螺杆菌鞭毛基因PCR-RFLP分析及其与胃十二指肠疾病关系的研究

目的探讨幽门螺杆菌(Helicobacter pylori)鞭毛基因(flaA和flaB)PCR—RFLP分型及其与胃十二指肠疾病的关系一方法用PCR技术分别扩增46株从消化性溃疡(25株)和非消化性溃疡(21株)患者胃黏膜中分离得到的H.pylori flaA和flaB基因，并用HpaⅡ和HindⅢ消化PCR扩增产物，琼脂糖凝胶电泳分离酶切片段，将酶切片段编码后输入SPSS10．0软件进行聚类分析．并比较不同的基因型别在溃疡组和非溃疡组的分布。结果46株H．pylori菌株flaA基因HpaⅡ和HindⅢ酶切后分别产生4种和3种酶切图谱；flaB基因HpaⅡ和HindⅢ酶切后分别产生1种和2种酶切图谱：综合flaA和flaB基因酶切结果，46株菌株可分为四个基因型别：但这四个基因型别在溃疡组和非溃疡组的分布差异无统计学意义(P=0．513)。结论应用PCR—RFLP方法可以将H.pylori fla基因分为不同的基因型别．但胃十二指肠溃疡和非溃疡组的菌株fla基因型别无差别。     

飞秒激光在剥脱综合症患者白内障手术中的应用

目的:观察应用飞秒激光治疗25例合并有剥脱综合症的白内障患者的临床效果.方法:采用Lensx飞秒激光联合白内障手术操作系统对合并有剥脱综合症的白内障患者预先进行准确的角膜透明切口、个性化的晶状体前囊膜切开及六块分裂方式进行预劈核,然后使用传统超声乳化行晶状体核乳化,注吸吸除残余皮质,挑选合适人工晶状体植入.根据囊袋松弛...     

中国成年人高血压患病区域聚集性及危险因素的多水平模型分析

Objective To analyze the region cluster and risk factors of hypertension in the Chinese adult population and to explore the application of multilevel regression model in the risk factors of hypertension. Methods Multi-stage random sampling technique was used to choose 15 540 individuals aged 35-74 years from 10 regions in China. Two-level logistic regression models were fitted under MLwiN 2.02 software. Results The region cluster of hypertension existed and variance portion coefficient was 3.1%. After adjusting for the age and gender, overall obese people (BMI≥28 kg/m2) were 4.50(95%CI: 4.00-5.06) times, overweight people (BMI=24-27.9 kg/m2) were 2.26 (95%CI: 2.07-2.46) times more likely to be hypertensive as compared with those of normal BMI (18.5-23.9 kg/m2), and those centrally obesive people (Waist circumference≥85 cm in male or 80 cm in female) were 2.62 (95%CI: 2.42-2.83) times more likely to be hypertensive as compared with those of normal WC. The age-and gender-adjusted odds ratios (Ors) of triglyceride (TG), serum total cholesterol (TC), glucose, low-density lipoprotein cholesterol (LDL-C) , high-density lipoprotein cholesterol (HDL-C) and drinking alcohol were 2.10 (95% CI: 1.89-2.33) , 2.08 (95% CI: 1.84-2.35) , 1.85 (95% CI: 1.60-2.14) , 1.58 (95% CI: 1.38-1.81), 1.49(95%CI: 1.32-1.69) and 1.15(95%CI: 1.05-1.27), respectively. Conclusion The prevalence of hypertension was not only affected by individual risk factors, such as obesity, drinking alcohol, abnormal glucose and serum lipids profile, but also affected by the geographic environment where people resided in. Population-and risk factors targeted strategies, proved a promising way to reduce individual risk of hypertension in the primary prevention of hypertension.     

河南省大气细颗粒物暴露与小于胎龄儿的发病风险关系

目的：探讨大气细颗粒物(PM2.5)及其组分与小于胎龄儿(SGA)发病风险的关联。方法：基于国家免费孕前优生健康检查数据库2013年河南省的母婴数据进行分析,使用logistic回归模型分析孕妇妊娠各时期PM2.5及其组分暴露对SGA的影响。结果：在调整一般人口学资料、生活方式和健康行为、妊娠情况和环境温度变量后,以第一分位数PM2.5浓度为参照,孕早期、孕中期、孕晚期和全孕期暴露于第四分位数PM2.5浓度分娩SGA的风险分别增加58.2%(OR=1.582,95%CI 1.44～1.738)、35.4%(OR=1.354,95%CI 1.235～1.483)、30.1%(OR=1.301,95%CI 1.189～1.423)和45.9%(OR=1.459,95%CI 1.363～1.561)。以第一分位数浓度暴露为参照,全孕期黑炭、有机物、铵盐、硝酸盐和硫酸盐第四分位数浓度暴露与分娩SGA风险增加均有关(P<0.05)。结论：妊娠各时期孕妇避免PM2.5暴露均能降低SGA发生风险。     

中国成年人高血压患病区域聚集性及危险因素的多水平模型分析

Objective To analyze the region cluster and risk factors of hypertension in the Chinese adult population and to explore the application of multilevel regression model in the risk factors of hypertension. Methods Multi-stage random sampling technique was used to choose 15 540 individuals aged 35-74 years from 10 regions in China. Two-level logistic regression models were fitted under MLwiN 2.02 software. Results The region cluster of hypertension existed and variance portion coefficient was 3.1%. After adjusting for the age and gender, overall obese people (BMI≥28 kg/m2) were 4.50(95%CI: 4.00-5.06) times, overweight people (BMI=24-27.9 kg/m2) were 2.26 (95%CI: 2.07-2.46) times more likely to be hypertensive as compared with those of normal BMI (18.5-23.9 kg/m2), and those centrally obesive people (Waist circumference≥85 cm in male or 80 cm in female) were 2.62 (95%CI: 2.42-2.83) times more likely to be hypertensive as compared with those of normal WC. The age-and gender-adjusted odds ratios (Ors) of triglyceride (TG), serum total cholesterol (TC), glucose, low-density lipoprotein cholesterol (LDL-C) , high-density lipoprotein cholesterol (HDL-C) and drinking alcohol were 2.10 (95% CI: 1.89-2.33) , 2.08 (95% CI: 1.84-2.35) , 1.85 (95% CI: 1.60-2.14) , 1.58 (95% CI: 1.38-1.81), 1.49(95%CI: 1.32-1.69) and 1.15(95%CI: 1.05-1.27), respectively. Conclusion The prevalence of hypertension was not only affected by individual risk factors, such as obesity, drinking alcohol, abnormal glucose and serum lipids profile, but also affected by the geographic environment where people resided in. Population-and risk factors targeted strategies, proved a promising way to reduce individual risk of hypertension in the primary prevention of hypertension.     

大鼠急性脊髓损伤后神经细胞凋亡及Fas和Cathepsin D的表达

目的探讨大鼠急性脊髓损伤后神经细胞凋亡及Fas、Cathepsin D的表达。方法 78只成年健康Sprague-Dawley(SD)大鼠,使用改良Allen氏法制作急性脊髓损伤模型,采用HE染色、原位末端脱氧核糖核酸转移酶介导DUTP标记法(TUNEL)对损伤脊髓组织进行标记;免疫组化方法测定Fas、Cathepsin D的表达变化。结果正常组、假手术组TUNEL、Fas、Cathepsin D阳性细胞较少见,损伤组TUNEL阳性细胞8 h明显增多,3 d达到高峰,7 d明显降低。Fas阳性表达的细胞也在8 h开始增高,3 d达到高峰,7 d下降。Cathepsin D阳性细胞数则在脊髓损伤后3 d明显增多,5 d达高峰,至观察时间点结束,未有明显衰减。结论 Fas、Cathepsin D均参与了脊髓继发性损伤的调节。