中国汉族人群PNPLA3rs738409SNP与原发性肝癌的相关性

目的研究中国汉族人群PNPLA3 rs738409 SNP与原发性肝癌发病的相关性。方法 217例原发性肝癌患者作为病例组,164名健康人作为对照组。病例组分为乙肝后肝癌组、丙肝后肝癌组及酒精性肝病后肝癌组。采用RT-PCR方法检测PNPLA3 rs738409位点的SNP。结果与对照组相比,病例组中PNPLA3 rs738409 G等位基因型频率明显升高(OR=9.313,P=0.002),其中乙肝后肝癌组及酒精性肝病后肝癌组与对照组的差异均有统计学意义(P分别为0.01及0.009)。结论 PNPLA3 rs738409 SNP在中国汉族人群中与乙肝后肝癌、酒精性肝病后肝癌的发生具有相关性。     

HBV感染后外周血CD4+CD25+Foxp3+调节性T细胞与疾病进展的相关性

目的:探讨HBV感染者外周血CD4~ CD25~ Fox- p3~ 调节性T细胞水平与HBV感染后疾病进程的相关性.方法:HBV感染者136名及健康对照40名,应用流式细胞仪胞内染色技术检测外周血CD4~ CD25~ Foxp3~ 调节性T细胞表达,结合HBV感染者临床情况进行分析.结果:HBV感染者外周血CD4~ CD25~ Foxp3~ 调节性T细胞表达率较健康对照组显著增高(7.48%±1.03% vs 3.58%±0.71%,P<0.01);慢性乙肝组与慢性重型乙肝组相比,外周血CD4~ CD25~ Foxp3~ T调节细胞的表达率有明显差异(6.55%±1.26% vs 8.65%±2.58%,P<0.05);HBV病毒载量的对数值与外周血CD4~ CD25~ Foxp3~ T调节细胞的表达率之间存在正相关(r=0.332,P<0.01).结论:HBV感染者外周血CD4~ CD25~ Foxp3~ 调节性T细胞水平与疾病进展明显相关.     

抗纤复方I号对乙醛刺激的肝星状细胞的细胞外基质及细胞因子分泌的干预作用

目的:研究乙醛对肝星状细胞(HSC) 细胞外基质和细胞因子分泌的影响及中药抗纤复方I号(KXI) 的干预作用.方法:采用大鼠肝脏原位灌流消化法获得并原代及传代培养HSC,大鼠灌以KXI 制备药物血清,以乙醛和药物血清作用于HSC,通过RTPCR 测定细胞内α1(I) 型胶原mRNA 的表达,以放射免疫法和ELISA 法...     

胃癌及癌前病变中PTEN及微卫星不稳定的变化及二者的关系

目的:探讨胃癌及癌前病变中抑癌基因PTEN和微卫星不稳定(MSI)的变化以及二者的关系.方法:胃癌及癌前病变标本取自中国医科大学附属第一医院内窥镜中心和肿瘤外科手术后,每组30例,PTEN杂合性缺失(LOH)和MSI应用PCR-SSCP银染色方法检测,PTEN基因突变通过PCR-SSCP ABI测序方法检测,S-P免疫组化检测PTEN蛋白质表达.结果:在萎缩性胃炎无肠化、萎缩性胃炎伴肠化、不典型增生、早期胃癌、进展期胃癌中PTEN LOH率和MSI率分别为10%、10%、13.3%、20%、30%和13.3%、16.7%、23.3%、36%、40%.PTEN蛋白质表达在正常胃黏膜为100%,在慢性浅表性胃炎、慢性萎缩性胃炎无肠化、慢性萎缩性胃炎伴肠化、中度不典型增生、重度不典型增生、早期胃癌、进展期胃癌中PTEN蛋白质的表达分别为96.7%、90.0%、76.7%、73.3%、63.3%、60.0%、43.3%.所有病例共检测到3例PTEN突变,这3例均为MSI阳性的进展期胃癌.结论:PTEN缺失及突变与胃癌的浸润转移关系密切,在胃癌的发病过程中,PTEN蛋白呈下调性表达,MSI是胃癌发生的早期分子标志,PTEN基因突变常发生在MSI阳性的进展期胃癌中.     

非酒精性脂肪性肝病患者血清瘦素和脂联素与胰岛素抵抗的关系

Objective To investigate the serum leptin and adiponectin levels in nonalcoholic fatty liver disease (NAFLD) patients, and their relationship with insulin resistance. Methods A total of 120 cases were enrolled and divided into two groups: NAFLD group (n = 60) and normal control group (n = 60). The serum levels of leptin and adiponectin were measured by EL1SA. The body mass index (BMI), waist-to-hip ratio (WHR), triglyceride (TG), total cholesterol (Tchol), high-density lipoprotein cholesterol (HDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GGT), fasting blood glucose (FBG) and HOMA-IR (homeostasis model assessment insulin resistance) were detected and analyzed. Results Compared with control group, the serum leptin level in NAFLD group was Significantly higher [(12.37 ± 1.99) μg/Lvs (5.20 ± 1.03)μg/L,P<0.01], while the serum adiponectin level was significantly lower [(12.69 ± 2.83) mg/L vs (22.83 ± 4.61) mg/L, P < 0.01]. HOMA-IR was also much higher in NAFLD group than that in control group[(4.86 ± 0.63) vs (1.91 ± 0.41), P < 0.01]. Logistic regression analysis showed that leptin was positively correlated with WHR ( P = 8.175, P < 0.01), HOMA-IR ( P = 0.974, P < 0.01 ), FBG ( β = 0.564, P < 0.01). In contrast, adiponectin inversely associated with HOMA-IR ( β = -0.495, P < 0.01 ) and BMI ( β = -0.314, P < 0.01) respectively. Conclusions The increased serum leptin level and decreased serum adiponectin level in NAFLD patients independently associated with HOMA-IR.     

抗纤复方Ⅰ号对乙醛刺激的肝星状细胞增殖、胶原合成的干预

观察了中药抗纤复方I号(KX I)对乙醛刺激的大鼠肝星状细胞(HSC)增殖、胶原合成的影响,以期阐述KX I抗酒精性肝纤维化的部分作用机制。 一、材料与方法 1.药物与药物血清的制备:KXI(主要由丹参、黄芪、红花等十味药物组成)浓缩口服制剂,每ml含生药2.7 g。按11 ml/kg给Wistar大鼠灌服中药制剂,每天1次,持续给药1     

非酒精性脂肪性肝病患者血清瘦素和脂联素与胰岛素抵抗的关系

Objective To investigate the serum leptin and adiponectin levels in nonalcoholic fatty liver disease (NAFLD) patients, and their relationship with insulin resistance. Methods A total of 120 cases were enrolled and divided into two groups: NAFLD group (n = 60) and normal control group (n = 60). The serum levels of leptin and adiponectin were measured by EL1SA. The body mass index (BMI), waist-to-hip ratio (WHR), triglyceride (TG), total cholesterol (Tchol), high-density lipoprotein cholesterol (HDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transpeptidase (GGT), fasting blood glucose (FBG) and HOMA-IR (homeostasis model assessment insulin resistance) were detected and analyzed. Results Compared with control group, the serum leptin level in NAFLD group was Significantly higher [(12.37 ± 1.99) μg/Lvs (5.20 ± 1.03)μg/L,P<0.01], while the serum adiponectin level was significantly lower [(12.69 ± 2.83) mg/L vs (22.83 ± 4.61) mg/L, P < 0.01]. HOMA-IR was also much higher in NAFLD group than that in control group[(4.86 ± 0.63) vs (1.91 ± 0.41), P < 0.01]. Logistic regression analysis showed that leptin was positively correlated with WHR ( P = 8.175, P < 0.01), HOMA-IR ( P = 0.974, P < 0.01 ), FBG ( β = 0.564, P < 0.01). In contrast, adiponectin inversely associated with HOMA-IR ( β = -0.495, P < 0.01 ) and BMI ( β = -0.314, P < 0.01) respectively. Conclusions The increased serum leptin level and decreased serum adiponectin level in NAFLD patients independently associated with HOMA-IR.     

幽门螺杆菌感染与血液系统疾病的关系

幽门螺杆菌(Helicobacter pylori,H.pylori)在全球的感染率超过50%,是人类最常见的感染。除慢性胃炎、胃溃疡、十二指肠溃疡、胃癌等消化系统疾病外,血液系统疾病也被证实与H.pylori相关,包括缺铁性贫血、特发性血小板减少性紫癜、维生素B12缺乏症和黏膜相关组织淋巴瘤。以上血液疾病患者的H.pylori感染率均比正常人高,且进行H.pylori根除治疗后症状都得到不同程度的缓解。H.pylori直接或间接地通过不同机制影响或决定着4种血液系统疾病的发生。     

一种新生大鼠肝细胞分离改良方法的建立*

目的 尝试建立分离并获得稳定培养的新生大鼠原代肝细胞（HC）的简易方法。方法 采用多步酶消化法和组织块培养贴壁法分离大鼠原代HC,经低速离心、选择性贴壁培养并纯化肝细胞。结果 最初获得单细胞数量约1~2×10⁶个/只,经0.4%台盼蓝染色细胞瞬时活率>72%,单细胞悬液和组织块两种培养方法均在3~5 d发现细胞可长满瓶底的70%~80%,经传代纯化以后细胞经糖原染色和抗CK-18免疫组化染色鉴定,发现HC纯度>95%,细胞生长状态良好,可供一般实验室使用。结论 成功建立了获取原代培养HC的简易方法,细胞数量、纯度、活率较好,可供一般实验室实验研究。