高鱼蛋白对高血压大鼠的降压作用

流行病学调查发现格陵兰、日本Fuse及我国舟山等渔区居民高血压病患病率低于其它地区,Kromhout等认为这与大量食用海产食物有关。家森等给易卒中型自发性高血压大鼠喂以鱼蛋白饲料后其高血     

高效液相色谱法测定脂必妥胶囊中洛伐他汀含量

目的建立测定脂必妥胶囊中洛伐他汀含量的高效液相色谱(HPLC)法。方法色谱柱为Kromasil C18柱(250 mm×4.6 mm,5μm),紫外检测波长238 nm,流速1 mL/min,流动相为乙腈-甲醇-0.1%磷酸溶液(58∶5∶37)。结果洛伐他汀进样量在0.032～0.32μg范围内与峰面积线性关系良好,平均回收率为98.7%,RSD=2.25%(n=9)。结论该方法准确、简便、快速,可有效控制脂必妥胶囊的质量。     

银杏黄酮类对实验性染铅大鼠血铅、GSH及SOD水平的影响

铅对婴幼儿及儿童的危害远远高于成人，其主要表现在脑发育期低水平接触铅导致中枢神经系统不可逆能障碍。 近年来，铅的氧化性损伤机理引起了人们的关注，据报导铅可破坏机体氧化抗氧化平衡，导致自由基产生增加；抑制抗氧化性非酶和酶系统的活性，导致机体氧化性损伤。文献报道银杏黄酮类具有强的抗氧化功能，本实验旨在观察银杏黄酮类化合物对染铅大鼠的血铅、SOD和GSH水平的影响，为防治铅中毒提供实验依据。     

补血中药饮片微量元素煎出率的研究

为探讨微量元素与补血中药的关系,本文就补血中药及其水煎液中微量元素的含量作一研究,以揭示药效与微量元素之间的关系及可能的利用率。实验结果报道如下：材料与方法1仪器与试剂1．1VIDEO22原子吸收分光光度计。1．2＊＊＊0’、＊＊OS等试剂均为分析纯,所用水均为去离子水。1．3标准品为高纯金属或氧化物。ZN定步瞩2．1样品处理：取市售桔子、熟地、何首乌、枣仁、仙灵碑、巴葫天、剔除杂质,粉碎后用几何法缩分样品,留样待测。2．2水煎液制备：称取试样0．5g,加水150ml,煎二次,每次30min,合并滤液,水浴蒸发至于,待测。2．3…     

阔筋膜瓣移植结合Ⅱ期植皮修复手、足创面缺损的临床应用

随着交通和机械产业的发展,临床上出现了相当比例的手、足创伤患者,其中组织缺损发生较为普遍。对于该类创面的修复,从最早的腹部带蒂皮瓣修复到后期的多种类型游离皮瓣修复,修复的方式取得了极大的发展。其中股前外侧皮瓣由于血管蒂解剖位置稳定,质地较好,临床上运用广泛[1]。但是对于手背、足背这些外观美容的区域,它又有不可忽视的缺点:外观的臃肿,后期局部的毛发密集,这些给美容要求高的患者带来了很大的烦恼。自2008年起,我们在临床上选择14例患者,采用游离阔筋膜瓣移植结合Ⅱ期植皮修复,取得了满意的临床效果。     

闭合性锤状指切口改良与缝线排异

2006年2月～2009年12月,我们对9例闭合性锤状指患者进行切口改良手术,报道如下.     

常规MRI和1H-MRS在职业性锰暴露致中枢神经损害中的应用价值

Objective MRI and MR hydrogen proton spectroscopy (1H-MRS) were used to detect the abnormal signal and alteration of metabolites, in order to explore the efficacy of these method in evaluating the damages of central nervous system (CNS) induced by occupational manganese exposure.Methods Eighteen workers exposed to manganese without any manganism symptoms, 12 workers with slightly chronic manganese poisoning, and 19 healthy workers were scanned using routine MRI sequence and 1H-MRS.The blood manganese concentration was also collected for each subject.On cerebral axial T1 WI,the signal intensities of ipsilateral globus pallidus and frontal white matter were measured in the visually brightest area (try to select the signal homogeneous region), and the globus pallidus index (PI) was then calculated.The 1H-MRS data was calculated to get the values of the peak height of N-acetylaspartate (NAA), choline (Cho), inositol (mI) and creatine (Cr) and the ratios of NAA/Cr, Cho/Cr, and mL/Cr were also calculated.One way ANOVA was used to compare the values of PI, NAA/Cr, Cho/Cr, mI/Cr and MnB among the three groups, and the correlations between PI and the time span of manganese exposure or blood manganese concentration were analyzed by Pearson correlation analysis.Eight workers exposed to manganese were followed up one year, and their PI , NAA/Cr before and after follow-up were compared by t test.Results Fourteen of 18 cases exposed to manganese without any manganism symptoms showed symmetrically high intensity signal on T1 WI, while the T2 WI were normal.No high signal intensity was observed on T1WI in any of the healthy workers or manganese poisoning workers.We found that the average PI in manganese exposed group (1.16 ±0.09) was significantly higher (F =24.79 ,P =0.O00)than those of the poisoning ( 1.05 ± 0.07 ) and control groups ( 1.01 ± 0.05 ).The blood manganese concentration in manganese exposed group, the poisoning group and the control group were (0.051 ±0.024), (0.047 ±0.018 ), ( 0.043 ± 0.020 ) μg/ml respectively, which was not significantly different ( F = O.623, P =0.541 ) and did not exceed the upper limit of normal reference value ( < 0.10 μg/ml ).There was a significantly correlation between PI and the time span of manganese exposure ( r = 0.67, P = 0.002 ),however, there was no correlation between PI and blood manganese concentration ( r = 0.20, P = 0.427 ).Furthermore, the NAA/Cr ratio decreased variously in the manganese poisoning group ( 1.22 ± 0.07 ) which was significantly lower( F = 4.120, P = 0.023 ) than those of the poisoning( 1.33 ± 0.13 ) and control groups ( 1.31 ±0.13).No statistical significanees were found in the ratios of Cho/Cr and mI/Cr among these three groups(P>0.05).No obvious changes of the PI and NAA/Cr were found in the 8 manganese exposed workers after 1 year follow-up.Conclusion Manganese exposure could lead to the high intensity signal on T1 WI, therefore the increased PI may be the biomarkers of central nerve system damages caused by the occupational manganese exposure.     

一蒂双足趾组织组合移植修复复合性手创伤

目的探讨一种简便、有效的治疗复合性手创伤的方法。方法1995年始,对12例复合手外伤均采用一蒂双足趾组织移植修复;其中用改良甲瓣加第二足趾移植再造手4例,指、第二足趾双趾腹皮瓣移植修复6例,足背皮瓣加趾趾腹皮瓣移植修复2例。急诊一期修复5例,延迟修复4例,二期修复3例。术后均经系统的康复治疗。结果12例的皮瓣及再造指全部成活。2例(足趾、皮瓣各1例)在术后发生动脉危象,手术探查、处理后成活。术后1年随访,再造指外观良好,TAM平均为140°,指腹二点分辨觉平均为8mm。再造指对掌、对指正常3例,轻度受限1例。移植皮瓣无明显臃肿,二点分辨觉平均为12mm。结论一蒂双足趾组织移植只吻合一套血管系统,供养两块组织,手术简便、安全、实用。保证移植组织的双套供血回流系统,及保持虎口、指蹼间的正常跨度是手术成功的关键。     

高龄肺癌患者手术的护理

高龄肺癌患者一般是指年龄在 65岁以上的病人 ,由于非特异性免疫力下降及各器官的退行性改变 ,心肺功能绝大多数存在不同程度的功能衰退和减弱 ,手术治疗危险性大 ,并发症多 ,死亡率相对较高。故认真做好手术前后的护理是提高高龄患者手术成功的关键。湖北省肿瘤医院于 1995年1月～ 1998年 1月行手术治疗及护理 65岁以上高龄肺癌患者 38例。现报告如下。1　临床资料38例高龄肺癌手术患者中 ,男 2 8例 ,女 10例 ,年龄65～ 69岁 2 7例 ,70岁以上 11例 ,手术行肺癌切除 34例 ,其中肺叶切除 30例 ,全肺切除 2例 ,右下肺楔形切除 2例 ,4例行肺动…