血管内皮生长因子在阿霉素肾病大鼠中的表达及其意义

血管内皮生长因子(VEGF)具有增加血管通透性和促进新生血管形成的作用。但病理状态下它的生物学功能及在肾小球疾病发生发展中的作用尚不明确。我们观察了阿霉素肾病(ADR)大鼠血清、肾组织VEGF的表达及与尿蛋白的关系,以探讨VEGF在ADR发病中的意义。 一、材料与方法 1.实验动物与分组:SD雄性健康大鼠30只,体重180-220 g,随机分为3组:(1)A组:正常对照组(n=10),大鼠     

The Effect of Connective Tissue Growth Factor on Human Renal Tubular Epithelial Cell Transdifferentiation

Summary To investigate the role of connective tissue growth factor (CTGF) in transdifferentiation of human renal tubular epithelial cell (HKC),in vitro cultured HKC cells were divided into 3 groups: negtive control, low dose CTGF-treated group (rh CTGF, 2.5 ng/ml) and high dose CTGF-treated (rhCTGF, 5. 0 ng/ml). Then the expression of α-smooth muscle actin (α-SMA) were assessed by indirect immuno-fluorescence, and the percentage of α-SMA positive cells were assessed by flow cytometry. RT-PCR were also performed to examine the mRNA level of α-SMA. Upon the stimulation of different concentrations of rhCTGF, the expression of α-SMA were markedly stronger than that in negative controls. The percentages of α-SMA positive cells were significantly higher in the stimulated groups than that of negative controls (38.9%, 65.5% vs 2.4%,P<0.01). α-SMA mRNA levels were also up-regulated by the stimulation of rhCTGF (P<0.01). These results suggest that CTGF can promote the transdifferentiation of human renal tubular epithelial cells towards myofibroblast (Myo-F). ZHANG Chun, male, born in 1977, M. D., Ph. D. This work was supported by a grant from the Science & Technology Foundation of Hubei Province (No. 2003AA301C14).     

Effect of Two Different Iron Supplementation on the Quality of Life of Uremic Hemodialysis Patients

UREMIC RENAL FAILURE is a common chronicdisease. Hemodialysis therapy has increased thelifespan of uremic patients significantly. Patientquality of life (QL), however, is also an importantindicator of the effectiveness of the medical carethat patients receive and therapies that prolonglifespan may actually compromise quality of life.1In recent years, intravenous iron has been studiedas an adjunctive therapy for anemia in hemodialysispatients. Based on protocols published previously,we selected hemodialysis ...     

茑萝松的生药学研究

目的：为茑萝松质量标准的制定及开发利用提供依据。方法：采用生药学的方法对茑萝松原植物形态特征药材性状和显微鉴别及紫外光谱进行观察研究。结果：首次系统阐明了茑萝松的生药学特性。结论：为茑萝松的准确鉴别和质量控制提供了科学的依据和生药学资料。     

654-2、普鲁卡因、肝素联合股动脉注射治疗血管病变

35例下肢血管病变的糖尿病患者,给予654～210mg、普鲁卡因100mg、肝素50mg加生理盐水5ml股动脉注射,每日一次1,0次为一个疗程后,对前后超声数据进行统计分析。结果治疗后患者下肢麻木,疼痛及足凉等症状减轻或消失,彩色多普勒复查显示血液动力学指标明显改善,下肢动脉的内径增宽、频谱变窄及加速度/减速度比值减小（P〈0.01）。结论654-2、普鲁卡因、肝素联合股动脉注射治疗糖尿病下肢血管病变值得临床推广和应用。     

社区住院2型糖尿病患者240例慢性并发症分析

目的:分析社区住院2型糖尿病患者慢性并发症情况.方法:以张家港澳阳医院住院的2型糖尿病患者为研究对象,应用统一的调查表,按病历记录,分别收集住院患者姓名、性别、病程、年龄、糖化血红蛋白、血糖、血脂、相关病史等.结果:本组研究中社区住院的2型糖尿病患者住院患者微血管并发症以糖尿病视膜病病变具多,与病程、甘油三酯呈正相关,合并症中以高血压、高脂血症、脂肪肝占比率较大,分别为38.3%、25.4%、31.2%,合并感染2型糖尿病患者占23.3%.结论:社区住院的2型糖尿病患者需提高并发症筛查的检测水平,及早发现并发症,在控制血糖同时加强血脂控制,减少糖尿病视网膜病变的发生.     

CD2相关蛋白在肾病综合征中的表达及意义

目的观察不同病理类型的原发性肾病综合征(nephrotic syndrome,NS)患者肾小球足细胞中CD2相关蛋白(CD2AP)的表达,探讨其与足细胞损伤的关系。方法选取原发性NS患者54例,10例同期肾肿瘤切除患者正常肾组织作为对照。肾活检后常规染色观察肾脏组织病理改变,肾组织行免疫荧光法CD2AP和肾小球上皮细胞蛋白-1(GLEPP1)双重标记,对肾小球CD2AP的表达进行定位;分别用real time PCR和免疫组化SP法检测组织中CD2AP的表达,采用real time PCR检测nephrin的表达,透射电镜观察足细胞的结构变化,并定量测量足突密度。结果 (1)NS患者肾小球中CD2AP的表达及nephrin的表达下调,足细胞足突不同程度融合,足突密度降低。(2)病理表现为微小病变性肾病(minimal change disease,MCD)、局灶性节段性肾小球硬化(focal segmental glomerulosclerosis,FSGS)和膜性肾病(membranous nephropathy,MN)的NS患者CD2AP表达及nephrin表达较对照组明显降低,且CD2AP与nephrin表达呈正相关,病理表现为MCD和FSGS的NS患者CD2AP表达与足突密度呈正相关。结论本研究首次发现原发性NS患者肾小球足细胞中CD2AP的表达降低,且在MCD和FSGS中与足细胞病变程度相关,提示CD2AP低表达在足细胞病变为主的肾小球疾病中发挥重要作用。CD2AP有利于诊断足细胞病变的早期检测,对CD2AP表达减低进行早期干预可能有助于延缓疾病进展。     

DN患者血清Cys-C、尿mAlb和血小板GMP-140检测的临床意义

目的 探讨糖尿病肾病（diabetic nephropathy,DN）患者血清Cys-C、尿mAlb和血小板GMP-140水平的变化及临床意义.方法：应用放射免疫分析、胶乳增强免疫比浊法和生化法对32例DN患者进行了Cys-C、尿mAlb和血小板GMP-140的检测,并与35名正常健康人作比较.结果 DN患者血清Cys-C和尿液Alb及血小板GMP-140水平均非常显著地高于正常人组（P〈0.01）,且血清Cys-C水平与尿mAlb、血小板GMP-140水平呈显著正相关（r=0.5642、0.6038,P〈0.01）.结论 检测DN患者血清Cys-C、尿mAlb和血小板GMP-140水平的变化对了解病情、观察疗效和预后判断均有重要的临床价值.     

短暂性脑缺血发作两种抗血小板治疗方案的的疗效比较和分析

目的 比较阿司匹林联合氯吡格雷与阿司匹林治疗短暂性脑缺血发作(TIA)的疗效和安全性,分析两种治疗方案差异的可能原因.方法 选择该院神经内科收治的诊断明确的98例TIA患者,50例给予口服阿司匹林和氯吡格雷为治疗组,48例予口服肠溶阿司匹林为对照组,均持续6个月.结果 随访6个月,治疗组50例TIA发作在10 d内完全...     

用常规实验室检测数据判定肾脏病理的神经网络方法

目的通过临床上常规的血液和尿液的检查结果,运用人工神经网络（Artificial Neural Networks,ANN）预测肾脏病理情况。方法收集1998年～2005年有血常规、肝肾功能、血脂、免疫球蛋白补体、出凝血功能及24h尿蛋白检查并有肾穿刺病理诊断的92例,应用神经网络对肾脏反应增生、浸润和硬化程度的25项指标的预测。结果预测值和实际值之间无显著差异（P〉0.05）。结论人工神经网络对肾脏疾病中的肾脏病理情况的预测具有可行性和实用价值。