高效液相色谱法测定盐酸氨溴索注射液的含量

目的建立高效液相色谱法测定盐酸氨溴索注射液的含量的方法。方法色谱柱Kromasil C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.05 mol.L-1磷酸二氢钾(含0.2%三乙胺,用磷酸调pH值=3.5)(25∶75),检测波长为245 nm。结果盐酸氨溴索在30~180 mg.L-1范围内线性关系良好,其回归方程为A=29128C-8748.4,r=0.9998,平均回收率为99.82%(n=5)。结论该法准确,快速,回收率高,适用于盐酸氨溴索注射液的含量测定。     

罗格列酮对单侧输尿管梗阻大鼠CTGF和TGF-β1表达的影响

目的 探讨过氧化物酶体增生物激活受体-γ激动剂-罗格列酮对单侧输尿管梗阻(UUO)大鼠肾间质转化生长因子β1(TGF-β1)结缔组织生长因子(CTGF)表达的影响.方法 将30只雌性Wistar大鼠随机等分成罗格列酮治疗组、模型组和假手术组.对治疗组、模型组行单侧输尿管结扎术建立输尿管梗阻动物模型.术后第21 天取3组术侧肾组织做HE和Masson染色观察肾组织病理改变,同时用免疫组化方法检测TGF-β1和CTGF在肾组织的表达水平.结果 罗格列酮可显著下调TGF-β1和CTGF的表达,改善了肾脏病理改变.结论 罗格列酮可下调UUO肾间质中TGF-β1和CTGF的表达,对UUO所致的肾间质纤维化的形成有抑制作用.     

抓住机遇 扎实工作 努力把卫生工作推上新台阶

2005年是国民经济和社会发展“十五”规划的最后一年，今年湖北省卫生工作的重点是抓好以下七个方面的工作：     

Expression of Serum and Glucocorticoid-inducible Kinase1 in Diabetic Rats and Its Modulation by Fluvastatin

The expression of serum and glucocorticoid-induced protein kinase in the renal cortex of diabetic rats was examined, and the function of signal transduction mediated by SGK1 in diabetic nephropathy and its modulation by fluvastatin were also investigated. 24 male Wistar rats were randomly divided into normal control group （n = 8）, diabetic nephropathy group （n = 8） and fluvastatin-treated diabetic nephropathy group （15 mg/kg/d, n=8）. The metabolic parameters were measured at the 8th week. The expression of transforming growth factor β1 （TGF-β1） and fibronectin （FN） was immunohistochemically examined. The expression of SGK1 was detected by RT-PCR and Western blot, and CTGF mRNA was assessed by RT-PCR. As compared to DN, blood glucose, 24-h urinary protein, Cer and kidney weight index were all decreased and the weight was increased obviously in group F. At the same time, mesangial cells and extracellular matrix proliferation were relieved significantly. The levels of cortex SGK1 mRNA and protein were up-regulated, and both TGF-β1 and FN were down-regulated by fluvastatin. The mRNA of SGK1 was positively correlated with the CTGF, TGF-β1 and FN. SGK1 expression is markedly up-regulated in the renal cortex of DN group and plays an important role in the development and progress of diabetic nephropathy by means of signal transduction. Fluvastatin suppressed the increased SGKlmRNA expression in renal cortex and postponed the development of diabetic nephropathy.     

CD2AP在足细胞中的生理功能研究

CD2相关蛋白(CD2 associated protein,CD2AP)是新近发现的足细胞裂孔隔膜(slit diaphragm,SD)分子。近年来的研究发现,CD2AP在足细胞内与多种结构蛋白相互作用,参与了足细胞多种重要的生理过程,并在蛋白尿的发生、发展中发挥着重要作用。文章综述了CD2AP的分子结构及其在肾脏足细胞中的表达分布,深入探讨CD2AP的生理功能及作用机制。     

以科学发展观指导农村卫生改革与发展

农村卫生是农村工作的重要组成部分,做好新时期的农村卫生工作是解决好“三农”问题,促进城乡协调发展,经济与社会协调发展,维护农村社会稳定,全面建设小康社会的重要措施,对于提高农民健康水平,保护农村生产力具有十分重要的意义。今年,湖北省卫生厅确立了树立科学发展观,促进农村卫生事业可持续性发展的整体思路,把农村卫生改革与发展摆到更加突出的位置,重点做好以下几个方面的工作。1　统筹规划,促进农村卫生的协调发展　改革开放以来,湖北省农村卫生事业在省委、省政府的领导下虽然取得了长足发展,但是,从整体上看卫生事业的发展与经济…     

肾组织肥大细胞的形态学特点及染色方法

目的探索检测肾组织肥大细胞的理想方法，研究肾组织中肥大细胞的分布和形态特点。方法用免疫组织化学方法和4种不同pH值的甲苯胺蓝染液对46例肾小球肾炎患者肾活检标本进行染色。观察肥大细胞在不同时间点的染出数及分布、形态特点，比较几种方法肥大细胞的显示数，并与免疫组织化学结果对照．计算每种染液的染出率。同时用透射电镜进一步观察肥大细胞的形态及超微结构。结果肥大细胞数量比较：pH0．5与1．0组无差别，其余的两两比较差异都有显著性．几种染液显示的肥大细胞相对于免疫组化结果的染出率分别是78．8％(pH0．5)．79．4％(pH1．0)，56．9％(pH3．0)．10．2％(pH5．0)。电镜观察到幼稚、成熟和脱颗粒的形态不一的肥大细胞。结论①免疫组织化学染色效果最好，其次是pH值0．5和1．0的甲苯胺蓝染液，pH5．0染液的效果最差。②肾组织肥大细胞形态多样．以缓慢形式脱颗粒。     

The Study on the Relationship between Serum Vascular Endothelial Growth Factor and Proteinuria in Adriamycin induced Nephrotic Rats

Abundant proteinuria,one characteristic ofnephrotic syndrome,notonly can cause the nephroticsyndrome and induce variouscomplications ofnephriticsyndrome,but also influence the progression ofestablished glomerular lesions. In normal kidneys,vascular endothelial growth factor (VEGF) ispredominantly expressed by glomerular podocytes,butits biological function and the role in the developmentof renal disease are obscure. This study was designedto evaluate the correlation between serum VEGF andpr…     

粉防己碱和尼莫地平对原发性高血压患者肾血流动力学的影响

采用单盲、随机和交叉的方法，观察粉防己碱（200mg3／d，po，4w）和尼莫地平（40mg3／d，po，4w）对30例原发性高血压患者的肾血流动力学的影响。有效肾血浆流量按I-邻碘马尿酸核素法测定，肾小球滤过率用生化法测定24h肌酐清除率进行评价。结果：两药均使血压显著降低（P＜0.01）。粉防己碱使有效肾血流量明显升高（P＜0.05），尼莫地平亦有增高趋势（P＞0.05）．两药均能有效地降低肾血管阻力（P＜0.01），但对肾小球滤过率和滤过分数无明显改变（P＞0.05）。它们对血压和有效肾血浆流量的影响无相关性。两药对全身和肾血流动力学的影响无显著差异（P＞0.05）。提示粉防己碱和尼莫地平均能扩张肾血管，改善肾循环，而后者弱于前者。