一氧化氮-氟比洛芬的胃肠毒性及其对胃粘膜前列腺素E_2的影响

目的　观察一氧化氮—氟比洛芬 (NO Flurbiprofen ,NO Flur)对大鼠胃肠的毒性以及对胃粘膜前列腺素E2 (PGE2 )的影响。方法　在解剖显微镜下分别观察大鼠胃内灌注NO Flur及氟比洛芬后 ,胃、小肠粘膜损伤指数及再进食胃窦溃疡模型溃疡面积 ,用EIA法检测胃粘膜PGE2 含量。结果　NO Flur的胃粘膜及小肠粘膜损害明显低于氟比洛芬 ,NO Flur组的胃窦溃疡面积稍低于氟比洛芬组 ,但无统计学意义。两药对胃粘膜PGE2 含量的抑制率无显著性差异。结论　NO Flur抑制胃PGE2 的程度与氟比洛芬相似 ,但胃肠毒性较轻     

褪黑激素对胃黏膜的保护作用及其机制

目的：探讨褪黑激素和血清素对鼠胃酒精性溃疡及黏膜血流的影响。方法：制备胃在体动物模型。分别皮下注入血清素及同容积生理盐水。用褪黑激素及同容积蒸馏水作为浸育液分别放入各组鼠的胃腔中。30min未毕毕胃黏膜血流后,采用40％的酒精作为浸育液分别放入各组鼠胃腔中,继续检测胃黏膜血流并测量胃黏膜损伤指数。结果：褪黑激素和血清素两者均不能损伤鼠胃黏膜,但血清素可降低胃黏膜血流,且与其剂理相关。褪黑激素可减轻由酒精引起的胃黏膜损伤和胃黏膜血流减少,而血清素可加重酒精引起的胃黏膜损伤和胃黏膜血流减少,但可被褪黑激素部分逆转。结论：胃黏膜的损伤与胃黏膜血流有关,但不是溃疡形成的唯一因素。因此,能拮抗血清的褪黑激素可作为在胃肠道作用的调节剂。     

植物多糖在胃肠道及肝病中的新作用(英文)

Ｔｈｅｐｏｌｙｓａｃｃｈａｒｉｄｅｆｒａｃｔｉｏｎｆｒｏｍｖａｒｉｏｕｓｈｅｒｂａｌｍｅｄｉｃｉｎｅｓｈａｓｂｅｅｎｗｅｌｌｓｔｕｄｉｅｄｆｏｒｉｔｓｉｍｍｕｎｏｍｏｄｕｌａｔｏｒｙａｃｔｉｏｎａｎｄａｎｔｉｔｕｍｏｒｅｆｆｅｃｔ,ｓｕｃｈａｓｌｅｎｔｉｎａｎａｎｄｌｕｃｉｄＧａｎｏｄｅｒｍａ ,ｆｏｒｍｏｒｅｔｈａｎｔｗｅｎｔｙｙｅａｒｓ .Ｔｈｅｓｅａｃｔｉｏｎｓａｎｄｅｆｆｅｃｔｓｍａｙｂｅｄｕｅｔｏｓｔｉｍｕｌａｔｉｏｎｏｆｔｈｅｐｈａｇｏｃｙｔｉｃａｃｔｉｖｉｔｙａｎｄａｎｔｉ ｉｎｆｌａｍ…     

顺铂降低铜离子转运蛋白1表达诱导人食管鳞癌细胞耐药

目的诱导对顺铂耐药的人食管鳞癌细胞株,并进一步研究细胞耐药性产生的机制。方法逐渐增加顺铂浓度处理人食管鳞癌细胞HKESC-1,诱导顺铂耐药细胞株HKESC-1/cis。MTT法观察顺铂的细胞毒作用以及细胞的生长曲线。电感耦合等离子体质谱检测细胞内顺铂的蓄积和Pt-DNA加合物的形成。Western blot检测铜离子转运蛋白(copper transporter 1,CTR1)的表达。结果顺铂耐药细胞株HKESC-1/cis较其亲代细胞HKESC-1对顺铂引起的细胞毒作用敏感性下降,耐药指数为2.9。顺铂耐药细胞株HKESC-1/cis与其亲代细胞HKESC-1的生长速度相似。然而,HKESC-1/cis细胞内顺铂蓄积和Pt-DNA加合物的形成较HKESC-1细胞减少,并且CTR1蛋白表达水平降低。结论顺铂通过降低细胞膜CTR1蛋白的表达,抑制顺铂进入细胞,导致细胞耐药性的产生。     

表皮生长因子对胃粘膜保护作用的机理探讨

表皮生长因子对胃粘膜保护作用的机理探讨陈宝雯，林兆鑫，许伟武，陆照棠，曹之宪本文拟探讨表皮生长因子（ＥＧＦ）对鼠胃粘膜血流（ＧａｓｔｒｉｃＭｕｃｏｓａｌＢｌｏｏｄＦｌｏｗ，ＧＭＢＦ）的影响。１．材料和方法（１）动物准备：雄性ＳＤ鼠，随机分成移出组（Ｓ...     

唐古特大黄多糖及其组分的抗氧化损伤作用

目的 比较唐古特大黄多糖（TMP）对小鼠急性肝损伤的保护作用。并探讨其作用机制。方法 保护作用：小鼠190只,随机分19例。预先以ＴＭＰ１００,２００和４００mg.kg^-1,TMP-I50,100mg.kg^-1灌胃（ig)给药,２次.d^-1,5d;第６日用ＣＣl4(156mg.kg^-1)、Ｄ－Ｇal-N(800mg.kg^－１）和ＴＡＡ（５５０mg.kg^-1)腹腔注射（ip)复制小鼠急性肝损伤模型。并用生理盐水做对照,应用硫代巴比妥酸比色法测定肝组织ＭＤＡ含量,比色法测定血清的ＡＬＴ与ＡＳＴ活性,机制研究：Ｐ小鼠９０只,随机分９组,以ＴＭＰ２００mg.kg^-1,400mg.kg^-1,TMP-I50mg.kg^-1,100mg.kg^-1ig,ip约药,２次.d^-1,5d;应用硫代巴比妥酸比色法测定肝组织ＭＤＡ含量,ＤＴＮＢ直接法测定肝组织和血清中ＧＳＨ－ＰＸ的活性。结果 唐古特大黄多糖能显著提高ＴＡＡ引起肝损伤动物存活率,降低各模型组动物肝组织ＭＤＡ含量,降低血清中ＡＬＴ,ＡＳＴ水平,两种给药途径ig,ip均能使正常小鼠肝组织ＧＳＨ－ＰＸ活性增加,结论 唐古特大黄多糖对ＣＣl4,Ｄ－Ｇal-Ｎ和TAA引起的小鼠急性肝损伤具有明显的保护作用。TMP-I可能是其有效组分,唐古特大黄多糖抗肝损伤的作用可能与其抗氧化作用有关。     

Passive cigarette smoke increases apoptosis in the rat gastric mucosa through reactive oxygen species- mediated pathway

AIM: To investigate the relationship between cigarette smoke andapoptosis in the rat gastric mucosa and the mechanism involved.METHODS: SD rats were exposed to different concentrations ofcigarette smoking (0 ％, 2 ％, and 4 ％ ) once daily for differentone-hour periods (1, 3, 6, and 9 times). Apoptosis wasdetected with TUNEL method, and the activity of xanthineoxidase (XO ) in the rat gastric mucosa was measured.BESULTS: Passive cigarette smoke time- and concentration-dependently increased apoptosis in the rat gastric mucosa, whichwas accompanied by the increase of XO activity. When the     

植物多糖在胃肠道及肝病中的新作用

The polysaccharide fraction from various herbal medicines has been well studied for its immunomodulatory action and antitumor effect, such as lentinan and lucid Ganoderma, for more than twenty years. These actions and effects may be due to stimulation of the phagocytic activity and anti-inflammatory action. It is also found to increase hematopoiesis in the bone marrow and to promote proliferation of several types of hematopoietic precursor cells. It was also found to provide radioprotective effect by improving the recovery of hematopoietic function in animals. These findings suggest that the polysaccharides from plant origins have significant pharmacological action on cells with high proliferative capacity in the body. Recently, increasing evidence implicates that vegetal polysaccharides have similar protective and repairing activities in the gastrointestinal (GI) tract as the cells in this system also have the same biological property. In this review, we would like to update and summarize findings both in animals and in human pertaining to the pharmacological actions of polysaccharides from different vegetal sources on the GI and hepatic disorders including ulcer and cancer diseases.