异丙酚麻醉下行人工流产术的临床观察

我院自2004-2005年以来在异丙酚麻醉下行人工流产术400例取得了较好的效果，现报道如下：     

不明热的诊断

一、定义与分类不明热于 1961年被提出 ,主要依据以下三点 :①发热持续三周以上 ;②发热达 3 8℃以上 ;③入院三周内难以确诊。目前也有人建议将第三条改为“三日内难以确诊”的病例。近年来 ,随着疾病性质变化 ,将不明热大致区分为 :①典型的不明热 ;②医院内发生的不明热 ;③中性粒细胞减少的不明热 ;④与ＨＩＶ有关的不明热等几种类型。各类型包含的疾病不同 ,一般来讲 ,与典型的不明热相比 ,后三者之间不易鉴别。二、病因与过去相同 ,在不明热中 ,感染性疾病 (3 0 % 40 % )、恶性肿瘤 (2 0 % 3 0 % )、胶原病 (10 % 2 0 % )为三大发病原…     

INFILTRATING T LYMPHOCYTE ACTIVITY IN GASTRIC AND COLONIC CANCER AND EARLY DECIDUA

The subclasses of mononuclear ceU infiltrates and T cell activity in tumor tissue as weU as early decidua and placental villi were studied in situ to clarify the differences and similarities between tumor immunity and pregnancy immunity. Biopsies of muco- sal specimens from patients with chronic gastritis and colitis were used as controls. It was found that there were numerous inactive T lymphocytes in tumor iaterstitium and decidua whereas there were numerous activated T cells in inflammatory mucosa, and there were hardly any lymphocytes in tumor parenchyma and villous tissue. In other words, there is no effector - target contact in tumor immuni ty and pregnancy immunity, indicating certain simi- Iaritie.s between them.     

人脐带间充质干细胞获得和纯化及向软骨细胞分化能力的研究

Objective To explore the efficient way to purify and to enrich umbilical cord mesenchymal stem cells which have abilities to differentiate into chondrocytes. Methods Umbilical cord was acquired from new born infants and chopped into 1 mm2 cubics, then they were pretreated with colleganase and trypsin so as to get the mixed cell suspension. CD45(-),CD90(+) cells were acquired from the mixed cell suspension via FCM sorting and were cultured in the medium as umbilical cord mesenchymal stem PO cells. The PO cells were detached and passaged by multiplex collagenase NB4 and the positive rate of CD90 and CD45 of the P1-P3 cells were observeed via FCM ; the P3 cells were induced to differentiated into chondrocytes. Results FCM results showed that the positive rate of CD90 on P1, P2, F3 was (80.86 ± 7.85)%,(95.86± 3.28)%,(97.15± 1.43)%, while the positive rate of CD45 on P1, P2, P3 cells was (2.53 ±0.28)% and (0.97 ± 0.48)% and (0.05 ± 0.01)% respectively; P3 cells had differenciated into chondrocytes. Conclusion FCM presorting and Multiplex collagenase detachment procedures can swiftly acquire highly purified human umbilical cord mesenchymal stem cells which have the ability to differentiate into chondroeytes.     

改进的阴道施源器及腔内放疗中的应用

本文介绍一种改进的阴道施源器的制作方法和在后装放射治疗中的应用。     

腹部巨大肿块的CT定位及定性诊断

目的探讨腹部巨大肿块的CT表现,CT检查对病变的定位及定性诊断意义。方法回顾性分析经手术病理证实的7例腹部巨大肿块,分别记录患者年龄、性别及病灶大小、位置、囊实性成分、强化程度,分析病变的起源及性质,从而得出CT诊断。结果通过对病灶在年龄、性别、大小、位置、囊实性成分、强化程度方面比较总结分析,发现CT定位及定性诊断与病理几乎吻合,分别为左肾巨大血管平滑肌脂肪瘤、左卵巢巨大黏液性囊腺瘤、左卵巢巨大囊肿、子宫浆膜下巨大平滑肌瘤、左肾巨大肾母细胞瘤、巨大胆总管囊肿、巨大肠系膜囊肿各1例。结论腹部巨大肿块在儿童多起源肾、胆总管及肠系膜,而在中青年妇女多起源卵巢、子宫及肾。CT增强扫描、二维及三维重建成像对腹部巨大肿块具有一定的定位及定性诊断意义。     

经等离子体修饰并复合BMSCs的组织工程神经研究

目的探讨经等离子体修饰的PLGA神经导管复合BMSCs后修复大鼠坐骨神经缺损的疗效。方法取30只新生SD大鼠股骨及胫骨骨髓,培养获得原代BMSCs,并传至第3代。将PGA和PLA按90∶10摩尔比例混合,制备PLGA神经导管,对其进行等离子体修饰。取30只4周龄SD大鼠,制备右后肢坐骨神经1cm缺损模型。按修复材料不同随机分成3组(n=10),实验组:第3代BMSCs复合经等离子体修饰PLGA神经导管;对照组:第3代BMSCs复合普通PLGA神经导管;自体组:自体神经。术后6周观察大鼠的行走步态变化,测定坐骨神经功能指数(sciatic function index,SFI)、电生理变化、腓肠肌湿重恢复率,采用再生神经轴突图像分析评价神经修复效果。结果术后大鼠均存活至实验完成。术后6周,实验组和自体组大鼠行走步态恢复情况较对照组好。实验组、对照组及自体组SFI分别为—51.02±6.54、—58.73±7.87及—48.73±3.95,组间比较差异均有统计学意义(P<0.05)。实验组运动神经传导速度及波幅明显高于对照组(P<0.05),但与自体组差别不明显(P>0.05),对照组与自体组差异有统计学意义(P<0.01)。实验组、对照组及自体组腓肠肌湿重恢复率分别为56.13%±4.27%、43.14%±6.52%、59.47%±3.85%,组间差异均有统计学意义(P<0.05)。实验组有髓神经纤维密度、直径及髓鞘厚度均高于对照组(P<0.05),低于自体组(P<0.05);对照组与自体组差异有统计学意义(P<0.01)。结论复合BMSCs的等离子体PLGA神经导管能有效促进周围神经的再生,为研制新型的组织工程神经修复周围神经缺损提供新的思路。     

香港高危青少年对滥用咳药水的信念

目的:探讨香港青少年对滥用咳药水的信念。方法:应用《滥用咳药水信念量表》(Beliefs about Cough Medicine Abuse Scale-BACMAS),了解160名滥用咳药水及65名非滥用咳药水的香港青少年,对滥用咳药水的信念。结果:研究显示,此量表有高的内在一致性信度及效度(包括同证效度及构念效度)。一般而言,参加者认为滥用咳药水并不会带来很多好处,而他们亦了解滥用咳药水的危害。不过,仍然有为数不少的参加者认为,长期滥用咳药水并不会上瘾,而他们亦认为跟滥用咳药水的朋友交往并不危险。结论:此量表具有可接受的心理测量特性。青少年对滥用咳药水的自相矛盾信念,对预防滥用咳药水的工作有重要的启示。