计算机在放射生物学中的应用 Ⅲ.克隆分析仪与计算机配套使用(简报)

克隆培养是离体细胞实验的最常用方法,它可以用于观察肿瘤细胞的化疗,放疗,放射增敏剂,放射防护剂以及各种影响肿瘤细胞生长因素的效应。由于克隆的计数、原始数据的处理.各条剂量～效应曲线的绘制以及求出各个实验参数的数值,利用小计算器计算需要很多时间。作者用OKI,if-800,model 50微机(日产),以扩展BASIC语言编写了一套计算及绘图程序,将克隆计数器的计数结果直接输入计算机进行数据处理,其中包括统计分析、参数Do·Dq·N值的计算、曲线的拟合,曲线的绘制及OER、SER     

川芎嗪对脑缺血再灌注损伤后细胞粘附作用的影响

背景:大脑缺血区白细胞与内皮细胞的活化及表面粘附分子的表达可使白细胞聚集浸润,发挥细胞毒作用进而使脑损伤加重。中药川芎嗪具有抗血栓形成,改善微循环的作用。目的:观察川芎嗪对脑缺血鄄再灌注损伤后局灶区白细胞与内皮细胞粘附性变化的影响。设计:随机对照动物实验。单位:第三军医大学大坪医院脑血管病二科,玉溪市第二人民医院精神科。材料:实验于第三军医大学大坪医院脑血管病二科实验室完成。雄性Wistar大鼠32只,体质量250～300g,随机分为3组,8只为正常对照组,12只为脑缺血鄄再灌注组,12只为脑缺血-再灌注加用川芎嗪组。方法:川芎嗪组静脉注射磷酸川芎嗪注射液(15g/kg)。正常对照组及脑缺血组予静脉注射等量生理盐水。通过免疫荧光标记技术和显微超高速录像系统观察脑缺血-再灌注及应用川芎嗪后局灶区白细胞粘附性的变化。主要观察指标:①脑缺血鄄再灌注损伤局灶区微动脉白细胞附壁密度指数及白细胞与内皮细胞之间黏附力。②应用川芎嗪后局灶区白细胞粘附性的变化。结果:实验动物全部进入结果分析。①与对照组比较,脑缺血鄄再灌注损伤组局灶区微动脉白细胞附壁密度指数增大,白细胞与内皮细胞间断裂应力明显下降,粘附力显著增强,差异均有显著性(P<0.01)。②与脑缺血鄄再灌注损伤组比较,川芎嗪治疗组白细胞附壁密度指数及粘附力显著下降,至24h后降至较低水平,断裂应力增高,至24h与正常对照组差异无显著性,并维持在较高水平。结论:川芎嗪可显著减轻脑缺血鄄再灌注损伤后内皮细胞与白细胞的粘附。     

2007年西藏自治区碘缺乏病高危地区重点人群调查分析

Objective To propose prevention and control strategy against iodine deficiency disorders to prevent new cases of endemic cretinism from occurring. Methods In April and August 2007, all the suspected cretinism patients born after the first of January, 1997 were searched for in 16 counties from Lhasa, Shannan, Nyingchi, Qamdo and Xigaze. Meanwhile, 60 children aged 8-10 years were selected in each of the two rural villages of each county chosen. Goiter was examined using palpation and B-ultrasound, urinary iodine was determined, inteligence quotient(IQ) was tested; 30 women of childbearing age from each village chosen underwent urinary iodine test and household salt semi-quantitative detection. Results No new cases of endemic cretinism had been found; Palpation identified goiter at 4.5% (257/5680) in children, B-ultrasound revealed a rate of 4.7% (258/5433). Median of urinary iodine was 159.4 μg/L, the averaged IQ was 78.3±14.5; women of childbearing age had a median urinary iodine of 70.2 μg/L. The coverage rate of iodized salt was 52.8%. Conclusions Goiter rate in children, urinary iodine level meet the standard set for the elimination of iodine deficiency disorders; however, the low IQ children, low coverage of iodized salt and the level of urinary iodine in women of childbearing age are less than desirable. Iodine deficiency disorders prevention and control need to be strengthened.     

眶下神经酚甘油与阿霉素阻滞治疗三叉神经痛的疗效比较

三叉神经痛是一种顽固性疼痛疾病，在临床中的治疗方法较多，但疗效不确切，早期内科治疗基本无效。神经毁损疗法成为治疗三叉神经痛的重要方法，但神经毁损性药物酚甘油的来源及保存困难。本文对眶下神经酚甘油与阿霉素阻滞治疗三叉神经痛的疗效进行了观察，现报告如下：     

传染性非典型肺炎患者凝血功能的变化

目的 观察传染性非典型肺炎(严重急性呼吸综合征,SARS)患者在发病急性期和治愈后不同时期的凝血功能改变。方法 检测在急性期和治愈后不同时期SARS患者血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、血浆纤维蛋白原含量(FIB)和血浆D-二聚体含量(DD)的变化。结果 与SARS恢复期相比,患者发病急性期的PT、APTT明显延长(P<0.01),FIB极显著升高(P<0.0001),DD含量显著升高(P<0.01)。急性期SARS患者血浆PT、APTT、FIB和DD异常的阳性率分别为31.8％、53.2％、80.9％和50％。结论 在SARS发病急性期,大部分患者存在高纤维蛋白原血症,约1/2的患者有凝血功能障碍和轻度继发性纤溶亢进,重症患者可并发DIC。SARS患者治愈后,凝血功能可逐渐恢复正常。     

动静脉内瘘的穿刺体会

血管通路是血液透析患者的生命线,动静脉内瘘是维持性血液透析患者最主要的血管通路[1],穿刺技术对动静脉内瘘的使用寿命至关重要.我科自1994年以来,对本科进行透析治疗的28例患者行动静脉内瘘术建立血管通路,共穿刺2286次,瘘管使用寿命最长为46个月,现将穿刺的体会总结如下.     

中老年胃癌108例分析

我院2003—01～2006—08共检出胃癌109例，其中40岁以上的中老年胃癌108例，现分析如下。 1临床资料 1．1一般资料本组资料来源于我院胃镜室，均为经电子胃镜检查并经病理活检证实。男84例，女24例，年龄40～84（平均68．5）岁。     

孕妇对孕期保健知识需求调查及施教效果分析

目的 了解孕妇在孕期对孕期保健知识的需求，探讨孕期宣教在围产保健中的积极作用。方法 采用问卷调查形式对孕妇做相关需求及孕期保健知识的掌握情况调查。结果 孕妇对孕期保健知识普遍需求，但对孕期保健知识的内容需求有显著性差异，接受孕期宣教前后对孕期保健知识、孕期自我监护、分娩的保健及母乳喂养知识、新生儿护理等掌握、态度及孕期心理有明显变化。结论 孕期宣教对围产期保健有积极的促进作用，护理人员应根据孕妇对保健知识的需求，改进和调整孕期宣教的结构，提高健康质量。     

脑血管造影后皮质盲三例

皮质盲是脑血管造影罕见的并发症,其病因尚不十分清楚。普遍认为,动脉造影后皮质盲与对比剂通过枕叶的血-脑屏障而导致枕叶脑细胞损害有关,该损伤较轻微,往往为可逆的[1-2]。内蒙古医学院附属医院自1997年1月至2006年4月共行全脑血管造影600例,出现明显视力改变者3例(0.5%)。将结果报道如下。1病例资料本组患者均在局部浸润麻醉下,采用Seldinger′s技术,行股动脉穿刺。穿刺后经股动脉鞘注入3000U肝素盐水。常规双侧颈总动脉及一侧或双侧椎动脉置管正侧位投照,必要时颈内、外动脉分别投照及旋转数字减影血管造影(DSA)。所用对比剂为碘海醇(…     

2007年西藏自治区碘缺乏病高危地区重点人群调查分析

Objective To propose prevention and control strategy against iodine deficiency disorders to prevent new cases of endemic cretinism from occurring. Methods In April and August 2007, all the suspected cretinism patients born after the first of January, 1997 were searched for in 16 counties from Lhasa, Shannan, Nyingchi, Qamdo and Xigaze. Meanwhile, 60 children aged 8-10 years were selected in each of the two rural villages of each county chosen. Goiter was examined using palpation and B-ultrasound, urinary iodine was determined, inteligence quotient(IQ) was tested; 30 women of childbearing age from each village chosen underwent urinary iodine test and household salt semi-quantitative detection. Results No new cases of endemic cretinism had been found; Palpation identified goiter at 4.5% (257/5680) in children, B-ultrasound revealed a rate of 4.7% (258/5433). Median of urinary iodine was 159.4 μg/L, the averaged IQ was 78.3±14.5; women of childbearing age had a median urinary iodine of 70.2 μg/L. The coverage rate of iodized salt was 52.8%. Conclusions Goiter rate in children, urinary iodine level meet the standard set for the elimination of iodine deficiency disorders; however, the low IQ children, low coverage of iodized salt and the level of urinary iodine in women of childbearing age are less than desirable. Iodine deficiency disorders prevention and control need to be strengthened.