玻璃离子减少桩道预备后根管微渗漏的实验研究

目的 研究桩道预备后用玻璃离子封闭根充物冠方对减少根管微渗漏的效果.方法 选取70颗离体单根管下颌前磨牙,阳性对照组5颗,阴性对照组5颗,A、B、C、D 4组各15颗.常规根管充填后,A、B、C、D 4组进行桩道预备,A组保留根尖4 mm根充物;B组保留根尖5 mm根充物;C组保留根尖3 mm根充物 1 mm Vitrebond玻璃离子;D组保留根尖4 mm根充物 1 mm Vitrebond玻璃离子.用葡萄糖微渗漏模型检测6组第1、2、 4、 7、 10、 15、 20、 25、 30天根管冠根向渗漏出的葡萄糖量.结果 A、B、C、D 4组第 1、 2、 4、 7 天微渗漏值差异无统计学意义(P>0.05);从第10天开始, A、B组微渗漏值均明显高于C、D组(P<0.05),而C、D组之间差异无统计学意义(P>0.05);A、B组微渗漏值在第20、 25、30天差异有统计学意义(P<0.05).结论 玻璃离子可以成功减少桩道预备后根管的微渗漏.     

FcγRⅢB基因多态性与汉族人群慢性牙周炎关系的研究

目的探讨FcγRⅢB基因多态性与慢性牙周炎敏感性的关系。方法收集63例重度慢性牙周炎患者、103例轻中度慢性牙周炎患者及80名健康对照者的颊黏膜拭子,提取DNA,采用等位基因特异性引物PCR(PASA)法测定FcγRⅢB基因多态性,比较各组间基因型分布的差异。结果FcγRⅢB基因型分布及等位基因频率在3组间差异无显著性。结论研究未显示FcγRⅢB基因型与慢性牙周炎相关。     

人釉原蛋白重组质粒PcDNA3-AMG在COS-1细胞系中的表达

目的研究人釉原蛋白(AMG)重组质粒PcDNA3-AMG在COS-1细胞系中的表达。方法采用脂质体载体法将釉原蛋白重组质粒PcDNA3-AMG导入COS-1细胞系,用Zeoin筛选得到稳定转染克隆,并经ELISA检测细胞内和细胞培养液中重组釉原蛋白AMG的表达。结果在未经转染的对照组细胞内和细胞培养液中均未检测到AMG的表达,而经转染的实验组不论细胞内或细胞培养液中均检测到AMG的较高表达,重组质粒PcDNA3-AMG转染组细胞内AMG浓度达0.253μg/ml。结论PcDNA3-AMG具有较强的在真核细胞系中表达和分泌AMG的能力,适宜基因工程体外制备重组釉原蛋白。     

PcDNA3/sIL-1RⅠ重组载体体外表达产物生物学活性的检测

目的：检测构建的PcDNA3／sIL-1R Ⅰ真核表达载体在体外的表达产物是否具有生物学活性。方法：脂质体介导PcDNA3／sIL-1RⅠ体外转染CHO细胞，MTT法检测重组质粒转染的CHO细胞上清液能否阻断IL-113生物学活性。结果：重组质粒转染的CHO细胞上清液中表达的sIL-1RⅠ能阻断不同浓度IL-1B抑制A375细胞生长的作用。结论：PeDNA3／sIL-1RⅠ重组质粒在体外导人哺乳动物细胞后能够表达具有相应生物学活性的目的产物。     

侵袭性牙周炎患者外周血CD-4+CD-25+调节性T细胞的检测及功能分析

Objective To investigate the changes of proportion and suppression function of CD-4+ CD-25+ regulatory T cells in the peripheral blood of patients with aggressive periodontitis.Methods Flow cytometric analysis was used to detect the frequency of CD-4+ CD-25+ regulatory T cells in the peripheral blood of 16 patients with generalized aggressive periodontitis and 17 patients with chronic periodontitis,as well as 17 periodontal healthy controls.Furthermore,CD-4+ CD-25+ regulatory T cells and CD-4+ CD-25- T cells were separated from peripheral blood of each enrolling subject using magnetic cell sorting technology.The direct suppression effect of CD-4+ CD-25+ regulatory T cells on CD-4+ CD-25- T lymphocytes proliferation was performed by the mixed lymphocytes reaction and measured by 3H-thymidine radioactive assay.Results The patients with generalized aggressive periodontitis had a lower frequency of CD-4+ CD-25+ regulatory T cells(9.71±4.01)%in the peripheral blood than periodontal healthy controls [(14.72±3.51)%]and chronic periodontitis patients[(17.01±5.16 )%],P＜0.05.A significant decrease was found in the suppression function of CD-4+ CD-25+ regulatory T cells from peripheral blood of patients with generalized aggressive periodontitis when co-cultured with CD-4+ CD-25- T lymphocytes in the proportion of 2:1,1:1 and 1:2 as compared with chronic periodontitis patients and periodontal healthy controls(P＜0.05).Conclusions Diminished numbers and decreased suppression function of CD-4+ CD-25+ regulatory T cells were found in patients with generalized aggressive periodontitis.     

侵袭性牙周炎患者外周血CD-4+CD-25+调节性T细胞的检测及功能分析

Objective To investigate the changes of proportion and suppression function of CD-4+ CD-25+ regulatory T cells in the peripheral blood of patients with aggressive periodontitis.Methods Flow cytometric analysis was used to detect the frequency of CD-4+ CD-25+ regulatory T cells in the peripheral blood of 16 patients with generalized aggressive periodontitis and 17 patients with chronic periodontitis,as well as 17 periodontal healthy controls.Furthermore,CD-4+ CD-25+ regulatory T cells and CD-4+ CD-25- T cells were separated from peripheral blood of each enrolling subject using magnetic cell sorting technology.The direct suppression effect of CD-4+ CD-25+ regulatory T cells on CD-4+ CD-25- T lymphocytes proliferation was performed by the mixed lymphocytes reaction and measured by 3H-thymidine radioactive assay.Results The patients with generalized aggressive periodontitis had a lower frequency of CD-4+ CD-25+ regulatory T cells(9.71±4.01)%in the peripheral blood than periodontal healthy controls [(14.72±3.51)%]and chronic periodontitis patients[(17.01±5.16 )%],P＜0.05.A significant decrease was found in the suppression function of CD-4+ CD-25+ regulatory T cells from peripheral blood of patients with generalized aggressive periodontitis when co-cultured with CD-4+ CD-25- T lymphocytes in the proportion of 2:1,1:1 and 1:2 as compared with chronic periodontitis patients and periodontal healthy controls(P＜0.05).Conclusions Diminished numbers and decreased suppression function of CD-4+ CD-25+ regulatory T cells were found in patients with generalized aggressive periodontitis.