EFFECT OF MOXIBUSTION OF DAZHUI(GV 14)ON CELLULAR IMMUNE FUNCTION IN TUMOR-BEARING MICE

Moxibustionisoneoftheimportantthera-peuticmethodsistraditionalChinesemedicine.Itswiderangingregulatoryeffectonorganismwasprovedbyalargenumberofpracticeandre-search.Withthemordernimmunologydevelop-ment,thestudiesoftheimmuneregulatoryef-fectofacupuncturemoxibustionhavebeenmorewidespreadanddeepgoinganditbecameimpor-tantwayofapproachingthemechanismintreat-ment.ofdiseasesbyacupuncture/moxibustion.Thevastamountofclinicalobservationshowedthatacupuncture/moxibustiontherapycouldal-leviatethesymptomofth…     

预灌封盐酸利多卡因注射剂质量检测方法及稳定性研究

目的建立测定预灌封盐酸利多卡因注射剂中药物含量和有关物质的HPLC法,进行方法学验证,并以所建方法考察了预灌封盐酸利多卡因注射液的稳定性。方法参考2020版中国药典,药物含量及有关物质测定均采用HPLC方法。药物含量采用安捷伦Eclipse XDB-C18色谱柱（4.6mm×250mm,5μm）,流动相为乙腈-磷酸盐缓冲液（60：40,V/V）,流速为1ml/min,进样量为20μL,检测波长254nm。药物有关物质测定,除流动相乙腈-磷酸盐缓冲液（50：50,V/V）,检测波长设定为230nm不同以外,其他色谱条件与药物含量测定方法一致。利用上述HPLC方法测定自制预灌封盐酸利多卡因注射剂中药物含量和有关物质,进行稳定性研究。结果含量测定方法在80.00~300.00μg/ml范围内线性关系良好（y = 0.0343x + 0.1627,r=0.9999）,平均回收率为101.38%（RSD=0.39%, n=9）。杂质2,6-二甲基苯胺与盐酸利多卡因可良好分离,分离度＞1.5,空白辅料不干扰测定,稳定性良好。结论建立的方法可用于盐酸利多卡因预灌封注射剂的含量及有关物质测定,方法简便、准确、灵敏度高、专属性强,为盐酸利多卡因预灌封注射剂的质量研究提供了定量分析的方法,且不同稳定性试验条件下制剂质量稳定可靠。     

莫让冻疮成为冬天的烦恼

冬天是养生的好时节。然而,冬天对于有些人来说也有难言的苦恼,如生冻疮。白晰的手背上冻痕累累;藏在鞋子里的脚趾、脚跟奇痒难忍;俏丽的耳朵又红又肿……为了不让冻疮成为冬天的烦恼,现在还是让我们来了解一下关于冻疮的知识,想办法永远地告别冻疮,健康舒心地度过冬天的日子。在民间,有"一年冻、年年冻"的说法,此话并不尽然。对于冻疮,只要积极预防和治疗,就可以免除皮肤之苦。容易生冻疮的人,从晚秋开     

同时性双原发胃癌 大肠癌7例分析

近8年作者收治7例同时患有胃癌、大肠癌且能手术切除病灶的病人,经术后病理证实为双原发癌;报道如下.     

肼苯哒嗪去甲基化作用对肿瘤基因影响的研究进展

肼苯哒嗪（Hydralazine,Hyd）是一种传统的用于心脑血管疾病治疗的药物,经长期临床应用发现其可致机体免疫性疾病毒副作用,其机制是Hyd的去甲基化效应所致。DNA甲基化（DNAmethylation）是哺乳动物最普通的一种复制后调节方式,其在个体发育、肿瘤发生、免疫紊乱中起着重要作用。近年研究表明,基因启动子区CpG岛（哺乳类生物基因组中的一段富含CpG二核苷酸成分的DNA序列）高度甲基化是多种肿瘤细胞中常见的变异,多个肿瘤抑制基因因其启动子区CpG岛甲基化而表达沉默,与恶性肿瘤的发生发展、侵润及转移密切相关。笔者现就近几年Hyd去甲基化效应用于抗肿瘤治疗的研究进展作一综述。     

口服人乳头瘤病毒18 E7与次级淋巴组织趋化因子联合基因疫苗的抗肿瘤作用

目的观察口服人乳头瘤病毒(HPV)18型E7联合次级淋巴组织趋化因子(SLC)基因疫苗的抗肿瘤作用。方法Western blotting方法鉴定稳定表达E7的B16F1细胞株。小鼠腋下接种稳定表达E7的小鼠黑色素瘤B16F1细胞,荷瘤小鼠随机分为5组(n=12),灌胃途径给予各种伤寒沙门菌液免疫小鼠:生理盐水组(生理盐水0.2mL)、空载体组(携带空载体的菌液0.2mL)、E7组(携带E7的菌液0.2mL)、SLC组(携带SLC的菌液0.2mL)和E7联合SLC组(携带E7或SLC的菌液各0.1mL)。检测各组小鼠免疫后的瘤重及生存率、血清及阴道分泌物E7特异性抗体产生情况、脾细胞增殖指数和杀伤活性,并观察肿瘤组织局部淋巴细胞浸润情况。结果稳定表达E7的B16F1细胞株成功构建。E7联合SLC组小鼠瘤重显著降低,生存期显著延长(P<0.05),诱导产生血清E7特异性IgG抗体水平显著增加(P<0.01),淋巴细胞增殖指数和细胞毒T淋巴细胞杀伤率显著高于其他组(P<0.05或P<0.01)。SLC组及E7联合SLC组肿瘤组织浸润的淋巴细胞数均明显增多。结论口服HPV18型E7联合SLC基因疫苗可发挥抗肿瘤作用,其机制可能为增强体液和细胞免疫应答。     

脑脉泰胶囊联合磷酸川芎嗪片治疗短暂性脑缺血发作疗效观察

目的观察脑脉泰胶囊联合磷酸川芎嗪片治疗短暂性脑缺血发作(TIA)的临床疗效。方法将462例TIA患者随机分成3组:脑脉泰胶囊组患者给予脑脉泰胶囊1.0g,口服,3次/d;磷酸川芎嗪片组患者给予磷酸川芎嗪片100mg,口服,3次/d;联合用药组患者给予脑脉泰胶囊联合磷酸川芎嗪片,用法及用量同上。治疗后比较3组患者的疗效。结果脑脉泰胶囊组、磷酸川芎嗪片组和联合用药组有效率分别为64.54%(91/141)、34.15%(56/164)、90.45%(142/157)。联合用药组的疗效最佳。结论脑脉泰胶囊联合磷酸川芎嗪片治疗TIA效果显著优于单独用药。     

小药片也能引发大出血

秦先生因牙痛吃了几片止痛药,谁知引发了胃出血,又是吐血,又是血便,人一下子就休克过去了。幸好家人及时把他送到了医院。经过抢救,终于将他从死亡边缘拉了回来。病情稍有稳定,医院给秦先生做了胃镜检查,发现他的胃部有多个溃疡出血病灶。家属感到很奇怪,虽然秦先生有过胃炎病史,但并不严     

1996～1999年福州市晋安区白喉免疫水平调查

为了解儿童白喉免疫水平 ,我们于１９９６～１９９９年对我区儿童白喉免疫水平进行调查 ,结果报告如下。１材料与方法１．１对象在我区每年抽检２～３个幼儿园 ,共抽检９所幼园 ,其中区属幼儿园５所 ,新成立的私立幼儿园４所 ,从中再随机抽检２～６岁的儿童１２２０人。１．２方法锡克氏试验进行抗体检测 ,用ＳＰＳＳ８．０统计软件建立数据库 ,并进行统计分析。１．３试剂采用上海生物制品厂生产的锡克氏液 ,有效期内使用。１．４结果判断用常规锡克氏试验结果判断方法。２结果本文共调查２～６岁儿童１２２０人 ,阴性８８３人 ,阴性率为７…     

塞来昔布对人鼻咽癌CNE-2细胞生长抑制及放射增敏研究

Objective To investigate the growth inhibition and radiosensitization of Celecoxib in hu-man nasopharyngeal carcinoma cell line CNE-2. Methods CNE-2 growth inhibition by Celecoxib was eval-uated by MTT method. Apoptosis-related changes in morphology were observed by transmission electron mi-croscopy (TEM). Cell cycle distribution and apoptosis rate were measured by flowcytometry (FCM). The ex-pression of COX-2 protein was observed by SP method after the treatment of Celecoxib. Cells were randomly planted into four groups: irradiation control(Ci), drug group(Cd), irradiation group(R), and Celecoxib plus irradiation group(D+R). Single irradiation of 2,4,6,8,and 10 Gy were administered for colonogenic assay. Cell cycle distribution and apoptosis rate were analyzed at 6 Gy irradiation. Results The growth of CNE-2 cell was inhibited by celecoxib in a dose-and time-dependent manner, the IC50 was 80 μmol/L After the treatment, cell ratio of GO and G, phases was increased (47.03±2.76 vs 56.17±1.95, t=4.68, P= 0.010), whereas the ratio of S and G2/M phases was decreased (33.07±1.86 vs 24.87±1.76, t=5.54, P = 0.010; 19.30±0.53: 17.73±0.83, t=2.75, P=0.050), and the apoptosis rate was increased (1.57±0.47:10.47±0.31, t = 27.39, P = 0.000) in a dose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage was found by TEM. SP method showed that Celeib decreased COX-2 expression (17.48±0.34 vs 12.82±0.51,t=13.20,P =0.00). The sensitivity ratio(D0) was 1.15. FCM showed that the percentage of cells in G2/M phase was significanty more in R and D+R groups than in Ci and Cd groups (68.00±1.65,54.27±5.74,17.60±0.80,14.86±1.23, t=47.70,P=0.000; t=11.63, P=0.000), and also significantly different between R group and D + R group (t=3.99, P= 0.020). The apoptosis rate was higher in R and D + R groups than Ci and Cd groups(4.83±0.97,9.50± 1.35,1.33±0.86 and 2.28±0.42,t=4.67,P=0.010;t=8.81, P=0.000), D + R group than R group(t =4.85,P=0.010). Conclusions Celecoxib can markedly inhibit the growth and induce apoptosis in CNE-2 cells,which may depend on COX-2 pathway. Celeeoxib potently enhances the radiosensitivity of CNE-2 cells,which may due to the repair inhibit of radiation-induced DNA damage, inhibit of cell proliferation,and enhancement of cell apoptosis after irradiation.