石家庄市宠物饲养者弓形虫感染状况及影响因素调查

弓形虫病是目前世界上流行广泛的人畜共患病之一。人体感染弓形虫后，免疫功能正常时表现为隐性感染。当宿主免疫功能下降时，可造成严重后果，孕妇患本病可造成流产、早产、死胎或胎儿畸形等。我国各省人群血清阳性率1．40％～38．65％，平均5．00％。为了解石家庄市宠物饲养者弓形虫感染情况及流行因素，对该地区部分人群进行了弓形虫感染的流行病学调查，现报告如下。     

回盲部假性息肉的声像图

<正> 回顾5年来,对临床申请的140例结肠炎患者,都做了水灌肠超声检查,在水的衬托下,在回盲部的位置上,显示出酷似息肉样强回声团块,其声像图显示率100％,140例患者,男62例,女78例,年龄19～75岁。超声检查用日本岛津SDL—300线阵实时超声显像仪,凸阵探头,频率3.5MHz。 1 观察与分析 经X钡灌肠及结肠镜对照,回盲部息肉均被排除,后又对401例经X钡灌肠及结肠镜证实的正常结肠患者,做了水灌肠超声检查,仍在回盲部显示息肉样强回声团块,经仔细、认真观察发现有以下特点:     

ROC曲线评价肝纤维化血清标志物的临床价值

检测了166例病毒性肝炎患者血清透明质酸(HA)、Ⅰ型胶原羧基端肽(PICP)、Ⅲ型胶原氨基端肽(PⅢNP)、Ⅳ型胶原(Ⅳ·C)及层粘连蛋白(LN)水平,并通过受试者特征工作曲线(ROC)评价了这些指标及其组合对慢性肝炎、肝硬变的诊断价值。结果除PICP外,其余指标均显著上升,且随CPH→CAH→LC浓度逐渐升高。通过ROC曲线下面积分析,PⅢNP及HA对CAH及CPH的鉴别诊断价值优于Ⅳ·C及LN,但是Ⅳ·C在CPH也有显著升高,比其它指标变化早。四个指标对肝硬变的诊断价值均无显著差异。应用重叠试验方法,以HA联合其它指标进行分析,结果HA+LN+PⅢNP组合最有价值,灵敏度65.2％,特异性达到了95.5％,临床有参考价值。     

大肠病变B型超声诊断现状

近年来,国内外已广泛地将B型超声用于大肠病变的诊断,应用得当有一定的参考价值,现将收集的有关文献作一综述。(一)B型超声检测出大肠病变可能性大量报告表明,B型超声对肠道病变有较高诊断价值,病灶大小在0.5～0.7cm即可检出。中晚期肠癌检出率     

如何提高小肾结石的B超显示率

如何提高小肾结石的Ｂ超显示率张宝珍，张文云，郭瑞军，蔡振奇，李莉，王秀芬，林海英作者自１９９２年以来，采用静脉注入速尿、利尿后再有实时Ｂ超仪、进行自身对照效果满意，报告如下。１临床资料本组８０例病人，其中男４５例、女３５例。年龄１６～５９岁。检查对象...     

细胞荧光素酶报告基因试验受CpG岛甲基化状态影响

Objective To investigate the effects of methylation status of CpG islands of endogenous E-cadherin (CDH1) gene on the promoter activity of corresponding genes in reporter assays. Methods The methylation statuses of CpG island of CDHI in 8 different cell lines were detected by methylation-specific PCR. CDH1 protein was analyzed by Western blotting. Two sets of pGL3 reporter vectors with different genotypes/haplotypes of the CDH1 promoter were constructed [pGI3-A(-73)/-C(-73)pGL3-H1/-H4]and used to transfect these cell lines. The differences between these promoter reporter vectors were analyzed by t-test. Results (1) CDH1 CpG island was unmethylated in AGS, MCF7, MKN74, and PC-3 cell lines,expressed in MCF7, MKN74, and PC-3 ,but not in AGS. Expression of CDH1 was silenced by methylation in HeLa, BGC823, A549, and RKO cell lines. (2) In the four CDH1 -unmethylated MCFT, M KN74, PC-3, and AGS cell lines ,the promoter activities of pGI3-C(-73)(as 0. 78±0. 10,0. 17±0.01,0. 11±0. 01,1.19±0. 18)were significantly higher than those of pGL3-A(-73)(as 0. 30±0. 08,0. 07±0. 01,0. 07±0. 01,0. 39±0. 04) (t values are -6. 298, -12. 349, -8. 128, -7.388, and P<0. 01). However, in the four C DH1 -methylated HeLa, BGC823, A549, and RKO cell lines, the promoter activity of pGL3-C(-73)(as 0. 09±0. 02,0. 13±0. 02,0. 05±0. 01,0. 01±0. 00) was significantly lower than that of pGL3-A(-73)(as 0. 16±0. 01,0.25±0.01,0. 11±0.03,0.03±0.00) (t valued at 5.958,11. 189,3. 661,13. 866,and P<0.05). (3) In the unmethylated MKN74 and methylated RKO cell lines, the promoter activities of pGI3-H1/-H4 were obviously and contrarily different(as 1.57±0. 23/0. 94±0. 06 and 0. 38±0. 02/0. 50±0. 04 ,t values were 4. 577 and -4. 915 ,P values were 0. 010 and 0. 003). Conclusion The methylation status of CpG island of the target gene in the tested cell lines affects the promoter activity in Reporter Assay significantly. The most active one may be the most suppressive one.     

机动车驾驶员的社会支持状况调查

随着我国经济的迅速发展，各类机动车的数量也在快速增加。机动车驾驶员已经成为一个庞大的特殊人群。本调查利用《社会支持评定量表》，从社会主观支持、社会客观支持及对社会支持的利用度3个方面调查机动车驾驶员生活和心理健康状况，为提高机动车驾驶员生活质量，获得更多有利的社会资源，更好地利用他人的帮助和支持提供依据。     

影响病房护患关系的因素及改进措施

护患关系是指在特定条件下，护理人员通过医疗、护理活动与患建立起的工作性人际关系。近年来，随着经济的发展，人们化生活水平的提高，健康观念的转变，住院患的自我保护意识也不断增强，护患矛盾呈上升趋势。笔通过对20年来的临床护理工作经验进行总结．归纳影响病房护患关系的主要因素，并针对具体情况采取适当措施，以适应“以病人为中心”的现代护理模式，建立良好的护患关系，提高医疗质量。[第一段]