STAT3在HPV16/18相关宫颈癌中的致病机制研究

目的 探究信号转导及转录激活蛋白3(STAT3)在人乳头瘤病毒(HPV) 16/18(HPV16/18)相关宫颈癌中的致病机制.方法 选取2015年6月至2020年6月本院收治的宫颈癌患者106例作为研究对象(试验组),选其术中切除的癌组织作为宫颈癌组.另选取同期因子宫脱垂、子宫肌瘤等疾病行子宫切除术的正常宫颈组织10...     

儿童高位胆管狭窄外科治疗探讨

高位胆管狭窄一般是指肝总管以上肝内胆管因炎症、肿瘤、损伤及先天性因素致胆管狭窄,结果因胆道梗阻使胆汁排出发生不同程度的障碍而导致肝功能损害。它是胆道外科处理上较为棘手的问题[2]。1992年至2004年我院收治疑难儿童高位胆管梗阻6例,其中行肝内胆管整形2例,肝脏切开、肝内深部胆管-肠管吻合术(Champeau术)3例,肝移植术1例,现报告如下。临床资料一、一般资料1992年8月至2004年12月我院共收治疑难高位胆管梗阻患儿6例,男4例,女2例;年龄4～13岁。其中2例因车祸肝破裂于当地医院行修补术后出现梗阻,2例肝内胆管结石,1例反复胆管炎引起…     

早期干预对足月小样儿神经行为及智能发育影响的临床研究

目的 探讨早期综合干预对足月小样儿神经发育的临床干预效果。方法 选取2017年10月至2018年6月安徽省妇幼保健院儿保科就诊的60例足月小样儿,按随机数字法分为医院干预组（30例）与家庭干预组（30例）,同期选取30例参加体检的足月非小样儿作为对照组。医院干预组采用医院干预模式,家庭干预组采用家庭干预模式。对比分析不同干预模式下小儿在不同发育时期的运动量表（PDI）评分和智力量表（MDI）评分情况。结果 出生后第42天时,对照组PDI和MDI评分分别为（80.20±13.93）分、（82.20±12.36）分,均高于医院干预组及家庭干预组,差异有统计学意义（P<0.05）;出生后12个月,医院干预组患儿PDI和MDI评分分别为（91.47±11.01）分、（95.37±11.66）分,接近对照组,差异无统计学意义（P>0.05）,但高于家庭干预组,差异有统计学意义（P<0.05）。重复测量方差分析结果显示,医院干预组PDI和MDI评分均高于家庭干预组,且随着干预时间延长,医院干预组两项评分增加幅度最大。结论 早期综合干预对足月小样儿神经行为及智能发育有明显的促进作用。     

贵州省地方性氟中毒地区人群GSTP1基因Ile105Val位点多态性分析

Objective To investigate plasma glutathione S-transferase(GSTs) activity and GSTP1 gene Ile105Val polymorphism in Bijie City, Guizhou Province, a coal-burning fluorosis endemic area. Methods One hundred and sixty villagers from Yachi Twon using non-improved cooking stoves were selected as the non-intervened group in Bijie City, Guizhou Province where coal-burning fluorosis was prevailing; 153 villagers as the intervented group were chosen from Changchun Twon, where cooking stoves were improved; 151 villagers were served as the control group from Baiyunshan Twon, Changshun County without endemic fluorosis. The activity of GSTs was tested by colorimetric analysis with spectrophotometer. The genotype of the GSTP1 gene Ile105Val polymorphism, presenting as either homozygous wild-type (AA), or heterozygous mutation type (AG), or homozygous mutation type (GG), was detected through the PCR-RFLP procedure. Results The activity of GSTs in plasma of non-intervened group [(12.44±4.97) kU/L]was significantly lower than that of intervened group (P < 0.05), and that of intervened group[(20.78±6.20)kU/L]was significantly lower than that of control group[(24.30±6.27)kU/L, P< 0.05]. The difference of the enzyme activity of three groups were statistically significant (F = 51.71, P < 0.05), but this enzyme activity did not vary significantly in each sex of each grnup(P > 0.05). Compared intervened group [AA:67.3%(103/153), AG:29.4%(45/153),GG:3.3%(5/153)]and non-intervened group[AA:66.9%(107/160), AG:30%(48/160), GG:3.1%(5/160)]with control group[AA:74.8%(113/151), AG:25.2%(38/151), GG:0 (0/151)], the Ile105Val polymorphism site of GSTP1 gene had significant difference(χ2= 6.04,6.07, both P< 0.05), but not significant between intervened and non-intervened groups(χ2 = 0.02, P>0.05). Conclusions Fluorosis can decrease the activity of GSTs and introduce the GSTP1 gene Ile105Val polymorphism, intervention with the fluorine intake will improve the effect of fluoride on the body.     

Condyloma（朗玛）研究——克尤瑞坦（医用生物凝胶敷料）联合外用盐酸氨酮戊酸散光动力治疗尖锐湿疣临床效果研究观察

目的 探讨克尤瑞坦联合外用盐酸氨酮戊酸散（aminolevulinic acid,ALA）光动力疗法治疗尖锐湿疣临床疗效。方法 选取2020年1月至2021年1月收治的湿疣患者142例作研究对象,随机分成治疗组、对照组,各71例,治疗组ALA光动力治疗后联合克尤瑞坦治疗,对照组ALA光动力治疗后联合α干扰素治疗,比较两组治疗前后临床疗效、疣体直径与疣体脱落率、HPV病毒清除率。结果 (1)对照组总有效率87.32%,治疗组95.77%,治疗组总有效率显著高于对照组（P <0.05）。(2)治疗组治疗后4、6、8、12周时疣体直径改善均显著高于对照组（P <0.05）。(3)治疗组总复发率7.04%,对照组19.72%,治疗组总复发率较对照组显著偏低（P <0.05）。(4)治疗组总不良反应发生率（4.23%）较对照组（19.72%）显著偏低（P <0.05）。(5)治疗组治疗6、8周时疣体脱落率（80.00%和95.90%）较对照组（64.43%和86.60%）显著更高（P <0.05）。(6)治疗组治疗炎性因子表达水平明显低于对照组（P <0.05...     

贵州省地方性氟中毒地区人群GSTP1基因Ile105Val位点多态性分析

Objective To investigate plasma glutathione S-transferase(GSTs) activity and GSTP1 gene Ile105Val polymorphism in Bijie City, Guizhou Province, a coal-burning fluorosis endemic area. Methods One hundred and sixty villagers from Yachi Twon using non-improved cooking stoves were selected as the non-intervened group in Bijie City, Guizhou Province where coal-burning fluorosis was prevailing; 153 villagers as the intervented group were chosen from Changchun Twon, where cooking stoves were improved; 151 villagers were served as the control group from Baiyunshan Twon, Changshun County without endemic fluorosis. The activity of GSTs was tested by colorimetric analysis with spectrophotometer. The genotype of the GSTP1 gene Ile105Val polymorphism, presenting as either homozygous wild-type (AA), or heterozygous mutation type (AG), or homozygous mutation type (GG), was detected through the PCR-RFLP procedure. Results The activity of GSTs in plasma of non-intervened group [(12.44±4.97) kU/L]was significantly lower than that of intervened group (P < 0.05), and that of intervened group[(20.78±6.20)kU/L]was significantly lower than that of control group[(24.30±6.27)kU/L, P< 0.05]. The difference of the enzyme activity of three groups were statistically significant (F = 51.71, P < 0.05), but this enzyme activity did not vary significantly in each sex of each grnup(P > 0.05). Compared intervened group [AA:67.3%(103/153), AG:29.4%(45/153),GG:3.3%(5/153)]and non-intervened group[AA:66.9%(107/160), AG:30%(48/160), GG:3.1%(5/160)]with control group[AA:74.8%(113/151), AG:25.2%(38/151), GG:0 (0/151)], the Ile105Val polymorphism site of GSTP1 gene had significant difference(χ2= 6.04,6.07, both P< 0.05), but not significant between intervened and non-intervened groups(χ2 = 0.02, P>0.05). Conclusions Fluorosis can decrease the activity of GSTs and introduce the GSTP1 gene Ile105Val polymorphism, intervention with the fluorine intake will improve the effect of fluoride on the body.     

基于网络药理学探讨莪术抗血栓作用机制

目的基于网络药理学探讨莪术抗血栓作用机制。方法利用TCMSP数据库获取莪术活性成分,从TCMSP和BATMAN-TCM数据库获取莪术活性成分靶点,利用Drug Bank等数据库收集血栓疾病基因,利用String工具构建蛋白互作网络,并运用Cytoscape软件构建莪术活性成分-靶点-疾病网络,借助DAVID工具进行关键靶点KEGG通路富集分析,使用Cytoscape Clue Go插件进行生物学功能分析。结果经条件筛选,莪术活性成分共29个,抗血栓靶点20个,包括F2、F10、PTGS1等。莪术抗血栓机制可能涉及凝血信号通路、血小板激活通路。结论莪术可能通过多成分、多靶点等相互作用方式,干预多条信号通路,进而调控血栓形成和发展。     

早期干预对足月小样儿神经行为及智能发育影响的临床研究

目的 探讨早期综合干预对足月小样儿神经发育的临床干预效果。方法 选取2017年10月至2018年6月安徽省妇幼保健院儿保科就诊的60例足月小样儿,按随机数字法分为医院干预组（30例）与家庭干预组（30例）,同期选取30例参加体检的足月非小样儿作为对照组。医院干预组采用医院干预模式,家庭干预组采用家庭干预模式。对比分析不同干预模式下小儿在不同发育时期的运动量表（PDI）评分和智力量表（MDI）评分情况。结果 出生后第42天时,对照组PDI和MDI评分分别为（80.20±13.93）分、（82.20±12.36）分,均高于医院干预组及家庭干预组,差异有统计学意义（P<0.05）;出生后12个月,医院干预组患儿PDI和MDI评分分别为（91.47±11.01）分、（95.37±11.66）分,接近对照组,差异无统计学意义（P>0.05）,但高于家庭干预组,差异有统计学意义（P<0.05）。重复测量方差分析结果显示,医院干预组PDI和MDI评分均高于家庭干预组,且随着干预时间延长,医院干预组两项评分增加幅度最大。结论 早期综合干预对足月小样儿神经行为及智能发育有明显的促进作用。     

静脉注射丙种球蛋白对过敏性紫癜患儿血清诱导血管内皮细胞表达TNF-α、诱导型一氧化氮合酶和一氧化氮的影响

目的 探讨急性期过敏性紫癜(HSP)患儿血清对血管内皮细胞表达TNF-α、诱导型一氧化氮合酶(iNOS)和一氧化氮(NO)的影响,以及静脉注射丙种球蛋白(IVIG)对上述各炎性因子表达过程的调节作用.方法 人脐静脉内皮细胞(ECV304)培养分为单独培养组、正常血清组、HSP血清组、HSP血清加IVIG组.分别应用RT-PCR法测定各组TNF-α、iNOS mRNA表达量,免疫印迹法测定各组TNF-α、iNOS 蛋白的表达量,硝酸还原酶法测定各组细胞培养上清中NO水平.结果 HSP血清组TNF-α、iNOS mRNA 和蛋白表达量,以及细胞上清中NO 表达量均较单独培养组和正常血清组明显升高(Pa<0.01);而HSP血清加IVIG组TNF-α、iNOS mRNA 表达量及TNF-α、iNOS蛋白表达量,细胞上清中NO 表达量均较HSP血清组明显降低(Pa<0.01).结论 急性期HSP患儿血清能诱导血管内皮细胞TNF-α、iNOS mRNA和蛋白表达量,以及细胞上清中NO表达增加,可能参与HSP患儿急性期血管损伤发生;IVIG可下调急性期HSP患儿血清诱导血管内皮细胞TNF-α、iNOS和NO的表达,提示IVIG防治血管损伤的机制可能与其抑制血管内皮细胞表达TNF-α、iNOS、NO有关.