尖锐湿疣患者外周血T淋巴细胞亚群检测结果分析

尖锐湿疣（CA）是目前国内外发病最多的性传播疾病之一，本病易反复发作，一般认为CA的复发与患者的细胞免疫功能有关。我们对20例体检健康人员及58例CA患者治疗前后的外周血T淋巴细胞亚群进行了检测，现将检测结果报告如下：     

铝对大鼠海马细胞内钙水平和钙通道蛋白基因表达的影响

Objective To dynamically investigate the effects of aluminum on the concentration of free intracellular Ca2+([Ca2+]i) and the expression of calcium channels in the hippocampus of rats. Methods Healthy 64 Wistar rats were taken as the experimental objects. And these rats were randomly divided into 16 groups according to their weights, and were instilled with AlCl3 at 0(control),37.3,74.7 and 248.7 mg/kg respectively. The experimental time exposed to AlCl3 was 45,75,120 d, among which the rats were given AlCl3 for 120 d fed normally for 30 d. The hippoeampus were segregated on day 45,75,120 and 150 d and the[Ca2+]i of hippocampus of rats were detected by fluorospectrophotometer. The expression of Ryanodine receptor 2 (RyR2) mRNA and α1C ubunit of L-type calcium ehannels(L-Ca2+α1C) mRNA were detected by RT-PCR analysis. Results [Ca2+]i was increased by AlCl3 in a dose-and time-dependant manner(F=23.136 and 19.089, P<0.01). There was a synergistic effect between the dose and time in [Ca2+]i (F=2.270, P<0.05). In time of 120,150 days, the [Ca2+]i of rats hippocampus in 37.3[(299.3±48.7), (342.7±35.3)nmol/L], 74.7[(391.2±47.9), (408.1±42.8)nmol/L] and 248.7 mg/kg group[(397.9±55.8), (405.2±22.7)nmol/L] significantly increased compared with control group [(195.1±29.9), (209.1±30.6)nmol/L; P<0.01]. The expression of RyR2 mRNA and L-Ca2+α1C mRNA were increased by AlCl3(F=23.301 and 60.812, P<0.01). The experimental time could lower the expression of L-Ca2+ α1C mRNA (F=6.088, P<0.01), but had no influences on the expression of RyR2 mR NA (F=1.361, P>0.05). There was interaction between the dose of AlCl3 and the time in the expression of L-Ca2+α1C mRNA (F=5.876,P< 0.01). On day 75,120 and 150 of the experiment, the expression of L-Ca2+α1C mRNA in rat hippocampus of 74.7 (1.03±0.16,1.18±0.18,0.92±0.11) and 248.7 mg/kg group(1.89±0.26, 1.25±0.10, 1.07±0.14) also increased compared with control group(0.63±0.09,0.78±0.16,0.69±0.11; P<0.05 or <0.01). On day 45,75, 120 and 150 of the experiment, the expression of RyR2 mRNA in 74.7(0.49±0.06,0.51±0.07,0.57±0.11, 0.47±0.11), 248.7(0.47±0.03,0.52±0.09, 0.70±0.10, 0.78±0.09)mg/kg AlCl3 groups was highly increased compared with control group (0.24±0.07, 0.32±0.04, 0.30±0.06, 0.27±0.06; P<0.05 or<0.01). Conclusion Al increases [Ca2+]i by increasing the expression of the RyR2 mRNA and L-Ca2+α1C mRNA, thus exerts an irreversible neuronal toxicity.     

端粒、端粒酶与恶性肿瘤

肿瘤是一种常见病、多发病,其中恶性肿瘤是人类最严重的一类疾病.近年来,端粒成为肿瘤分子生物学的研究热点,与癌症的发生、发展密切相关.本文拟对恶性肿瘤中端粒、端粒酶的主要研究进展和研究前景作一综述.     

中国自动接骨术——骨折的非固定治疗

中国自动接骨术又称为小中药疗法或骨折的非固定治疗。积数十年的临床经验，认为其不同于西医，也不同于中西医结合治疗骨折之观点，颇需要用第三只眼睛加以关注。     

车祸引起严重复合外伤96例抢救及护理

我院于1992年至1995年底共收治因车祸引起严重复合外伤病人96例,经过积极的抢救护理,其中89例痊愈出院,7例死亡,现抢救护理体会介绍如下:     

乙酰肝素酶、缺氧诱导因子-1α的表达与食管鳞癌浸润转移的关系

目的:探讨乙酰肝素酶(heparanase,HPA)及缺氧诱导因子-1α(hypoxia inducible factors-1α,HIF-1α)在食管鳞癌中的表达及其与食管鳞癌浸润转移的关系.方法:应用原位杂交技术检测70例食管鳞癌组织和23例切缘正常组织中HPA mRNA表达,同时应用免疫组化法检测HIF-1α蛋白在全部标本中的表达.结果:癌组织中HPA mRNA和HIF-1α的阳性表达率分别为65.7%(46/70)和55.7%(39/70),显著高于切缘正常组织(P＜0.05);HPA mRNA和HIF-1α的表达与食管鳞癌组织浸润深度、淋巴结转移有关(P＜0.05);在食管鳞癌组织中HPA mRNA的表达与HIF-1α的表达关系密切(Kappa=0.615,P＜0.05).结论:HPA和HIF-1α阳性表达与食管鳞癌的生长及浸润转移有关,两者在食管鳞癌的发生、发展过程中可能存在着一定的协同作用;检测HPA与HIF-1α表达有助于食管鳞癌生物学性能的判断.     

年龄相关性白内障术前A超/B超及眼电生理检查的护理

年龄相关性白内障的治疗目前多采用超声乳化白内障吸出人工晶体植入手术。术前对手术疗效的判定及视力恢复与术前详尽的眼部各项检查密切相关。而这类病人大部分以高龄伴全身多种疾病,对手术有畏惧感和对手术期望值高为特点,因此,给术前检查增加了难度。病人做好术前检查及在检查过程中给予积极主动的护理配合,为手术提供准确的眼部信息和数据至关重要[1]。2005年12月以来,我们对施行该术式的150例病人加强检查过程中的护理,效果满意。现总结如下。1临床资料本组150例,其中男72例,女78例。年龄45岁～95岁,平均年龄75岁。术前视力光感至0.4左…     

瘢痕子宫分娩方式的研究进展

综述瘢痕子宫再次妊娠分娩方式的选择对母婴结局的影响,探讨各种分娩方式的利弊,适应症,可行性及临床管理方案.