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放射免疫分析法(Radioimmunoassay,简称RIA)是1960年首先由Yalow和Berson创立的一种体外的超微量分析方法,它是将具有高灵敏度的放射性核素示踪技术与高度特异性的免疫化学技术二者结合而建立起来的新的分析方法。几乎一切 相似文献
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Intravenous supplement of essential amino acids (EAA) 12.6 g/d, fortified with branched-chain amino acids, was given to Group A, 5 patients with chronic renal failure (CRF) (mean creatinine clearance rate 6.55±2.49 ml/min) for 14 days on the basis of low protein diet (0.6 g/kg/d) , and Group B, 4 patients with CRF (mean creatinine clearance rate 9.75±3.93 ml/min) for JO days on the basis of low protein diet(0.4g/kg/d). The nitrogen balance, whole body nitrogen flux and rates of protein synthesis and catabolism and amino nitrogen utilization were markedly improved as compared with the pre-treatment levels. With treatment, the levels of some EAA in plasma such as valine, isoleucine, phenylalanine, lysine and methionine were increased, while that of tyrosine was not changed. In 4 of the Group A patients, blood urea nitrogen, creatinine and phosphate were slightly decreased after treatment, while serum calcium level was significontly increased. In 8 patients uremic symptoms were markedly ameliorated and their nutritional status apparently improved. 相似文献
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稳定性同位素~(15)N 标记氨基酸是一种对人体无辐射损伤的示踪剂,十分适合于人体体蛋白动态变化的研究,半个世纪来国外作了广泛深入的研究,并应用于临床。这方面的主要工作有:①营养不良患儿病愈前后及正常人在高蛋白和低蛋白饮食后体蛋白更新的研究:②手术前后体蛋白代谢动态变化研究;③感染性疾病体蛋白更新动态研究;④尿毒症及血透后体蛋白更新动态研究。尤其引入注意的是~(15)N 标记氨基酸是研究尿毒症饮食治疗的一种很有用的工具。~(15)N 示踪技术与氮平衡技术的比较蛋白质代谢的变化,临床上往往用氮平衡技术来研究。这种方法是通过测定摄入食物中的含氮量与尿及粪中的含氮量对比关系来估计人体总的氮的平衡情况。如排出量超过摄入氮量,则表示氮的负平衡;反之,若排出氮量小 相似文献
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