稳定转染ERα基因抑制MDA-MB-231乳腺癌细胞COX-2和VEGF-C的表达

Background Estrogen receptor （ER）-negative breast cancer cells are more aggressive than ER-positive cells. Elevated levels of cyclooxygenase-2 （COX-2） and vascular endothelial growth factor-C （VEGF-C） expression have been detected in cultured human breast cancer cells and are associated with negative hormone receptor status. In this study, we created ERα stable transfectants in MDA-MB-231 cells to explore the effect of ERα on cell growth and COX-2 and VEGF-C expression.Methods The green fluorescent protein （GFP）-ERα plasmids were stably transfected into ER-negative MDA-MB-231 cells. The proliferation and migration of untransfected MDA-MB-231 cells, ERα-transfected MDA-MB-231 cells and ER-positive MCF-7 cells were determined. The expression of COX-2, and the levels of VEGF-C mRNA and the VEGF-C secretion concentration were assayed in these cell lines.Results The proliferation and migration capacities of ERα-tranfected MDA-MB-231 cells were significantly decreased （P 〈0.05）. The expression of COX-2 was significantly lower in ERa-tranfected MDA-MB-231 cells than in untranfected MDA-MB-231 cells. The mRNA and protein levels of VEGF-C were lower in ERa-tranfected MDA-MB-231 cells than in untransfected MDA-MB-231 cells （P〈0.05）.Conclusions ERα stable transfection inhibits proliferation and migration capacities of MDA-MB-231 cells and decreases expression of COX-2 and VEGF-C. The decreases of proliferation and migration capacities may be related to suppression of COX-2 and VEGF-C expression.     

50例二氧化碳激光治疗酒渣鼻的体会

目的探讨二氧化碳激光聚焦点状治疗酒渣鼻的效果。方法对50例酒渣鼻患者中红斑期29例,丘疹期16例,鼻赘期5例进行二氧化碳激光聚焦点状治疗。结果29例经一次治疗患者面部红斑显著减轻,树枝状管消失,结节不同程度减轻;16例丘疹期患者红斑、结节不同程度减轻,树枝状血管减少,二次治疗后六例有轻微色素丢失;5例鼻赘期患者增生结节有改善,二次治疗后五例均出现轻度凹陷性疤痕。结论二氧化碳激光聚焦点状治疗酒渣鼻是较好的治疗方法,对于红斑期、丘疹期无瘢痕形成,鼻赘期激光操作时要仔细谨慎。     

微孔引流在大汗腺切除术治疗腋臭中的作用

目的 探讨微孔引流在腋窝皮下大汗腺切除术治疗腋臭中减少手术并发症的作用.方法 研究组为选择腋窝皮下大汗腺切除及微孔引流治疗患者,共51例;对照组为腋窝皮下大汗腺切除及胶片引流治疗患者,共58例,术后随访一年,观察两组患者术后疗效及并发症的发生.结果 研究组与对照组的术后疗效无明显差异,而研究组切口哆开及表皮坏死术后并发症的发生率显著低于对照组.结论 采用腋窝皮下大汗腺切除术及微孔引流的手术方法治疗腋臭,效果可靠,手术并发症少.