湖南汉族人群HLA-DP、-DQ位点等位基因多态性与鼻咽癌的相关性

目的 探讨湖南汉族人群HLA—DP、HLA—DQ位点等位基因多态性与鼻咽癌遗传易感性之间的关系。方法 应用聚合酶链反应—特异性寡核苷酸探针基因分型技术对87例湖南汉族鼻咽癌患者与91名健康对照作HLA—DPAl、—DPBl、—DQAl及—DQBl的基因分型，采用x^2检验比较两组各位点等位基因频率，单倍型频率分布的差异。结果 发现鼻咽癌组DPAl*0201、DPBl*1901、DQAl*0201较对照组明显降低，DPBl*0402、DQAl*0101较对照组明显升高；单倍型DPAl*0201—DPBl*1401及DQAl*0201—DQBl*0201较对照明显降低；但P值经Bonferroni校正后，差异均无显著性(Pc＞0．05)。结论 湖南汉族人群HLA—DP和—DQ位点与鼻咽癌无明显相关，与以往用受累同胞对方法在鼻咽癌家系中未能证实鼻咽癌易感基因与HLA—DP和—DQ位点连锁的结果一致。     

GSK-3及P70S6K在胰岛素抵抗大鼠肌肉中的表达及意义

目的:观察饮食诱导的胰岛素抵抗(IR)大鼠骨骼肌中糖原合成酶-3(GSK-3)及核糖体S6蛋白激酶(P70S6K)的表达及变化情况,并探讨GSK-3和P70S6K在IR发生中的作用及意义.方法:将40只4周龄Wistar大鼠随机分为正常对照组、高糖组、高脂组、高脂高糖饲养组,喂养8周后用高胰岛素-正葡萄糖钳夹技术(钳夹试验)对大鼠进行胰岛素敏感性的评估,采用Western blot法检测各组大鼠骨骼肌中GSK-3及P70S6K的含量,同时测定各组大鼠的附睾脂肪垫重量、血糖(BG)、胰岛素(INS)、甘油三酯(TG)、胆固醇(TC)及游离脂肪酸(FFA)水平、超敏C反应蛋白(hsCRP).结果:高脂高糖组、高脂组大鼠产生明显IR,体重增加(P＜0.01),以附睾脂肪垫的重量增加更为显著(P＜0.01),TG、TC及FFA水平、hsCRP增加(P＜0.01),GSK-3、P70S6K在IR大鼠肌肉中的表达明显升高.结论:高脂高糖饮食可诱导大鼠产生IR,IR大鼠的hsCRP、TG、TC及FFA水平明显增高,大鼠产生IR与炎症反应有关,GSK-3、P70S6K在IR大鼠中的表达明显升高,在胰岛素信号传导中起负相调节作用.     

XRCC1、hOGG1基因多态性与喉癌遗传易感性的关系

目的 探讨X线修复交叉互补组1基因(X-ray repair cross complementing group 1,XRCC1)、8-羟基鸟嘌呤修复酶基因(human 8-oxoguanine glycosylase I,hOGG1)多态性与喉癌遗传易感性的关系.方法 采用病例-对照设计,应用聚合酶链反应-限制性片段长度多态性分析法检测了72例经病理确诊的喉癌患者和随机抽样的72例无肿瘤、无遗传病对照者XRCC1-Arg399Gln、hOGG1-Ser326Cys多态性.结果 病例组XRCC1第399位密码子杂合型(Arg/Gln)及突变型(Gln/Gln)和hOGG1第326位密码子杂合型(Ser/Cys)及突变型(Cys/Cys)分布频率均高于对照组(P＜0.05),与携带XRCC1-399野生型(Arg/Arg)、hOGG1-326野生型(Ser/Ser)个体相比,携带该基因型的个体喉癌的发病风险分别升高了3.37和2.54倍.交互作用分析显示,吸烟组与不吸烟组相比,携带XRCC1、hOGG1各基因型的个体的喉癌发病风险差异未发现存在统计学意义(xH12=0.15,xH22=0.28,P＞0.05).结论 XRCC1-399位点Arg→Gln和hOGG1-326位点Ser→Cys的氨基酸替换可能导致喉癌的发病风险增加,XRCC1-Arg399Gln、hOGG1-Ser326Cys多态性可能与喉癌的遗传易感性有关.     

团体咨询消解大学生交流恐惧的研究

目的 :探讨团体咨询对消解大学生交流恐惧的有效性及可行性。方法 :以团体咨询的形式对 12名与人交流时存在恐惧或焦虑的大学生进行为期 9周的训练 ,实验前后分别测查PRCA - 2 4、PRCS、FNE、SAD及SCL - 90中的人际敏感、抑郁、焦虑和恐怖 4个分量表 ,同时通过主观评价量表判断团体咨询的效果 ,团体咨询结束 5个月后对被试予以追踪调查。结果 :经 9周团体咨询后 ,除FNE量表无显著差异外 ,其余量表及自评测量项目表现为明显改善 (P <0 .0 5 ) ,追踪评估结果提示团体咨询活动结束 5个月后效果依然存在。结论 :团体咨询方法对消解大学生交流恐惧是有效的、可行的     

Human adipose-derived adult stem cells produce osteoid in vivo

Adult subcutaneous fat tissue is an abundant source of multipotent cells. Previous studies from our laboratory have shown that, in vitro, adipose-derived adult stem (ADAS) cells express bone marker proteins including alkaline phosphatase, type I collagen, osteopontin, and osteocalcin and produce a mineralized matrix as shown by alizarin red staining. In the current study, the ADAS cell ability to form osteoid in vivo was determined. ADAS cells were isolated from liposuction waste of three individual donors and expanded in vitro before implantation. Equal numbers of cells (3 x 10(6)) were loaded onto either hydroxyapatite/tricalcium phosphate (HA-TCP) cubes or the collagen/HA-TCP composite matrix, Collagraft, and then implanted subcutaneously into SCID mice. After 6 weeks, implants were removed, fixed, and demineralized and sectioned for hematoxylin and eosin staining. Osteoid formation was observed in 80% of HA-TCP implants loaded with ADAS cells. Only 20% of Collagraft implants were positive for the presence of osteoid matrix. Whereas 100% of HA-TCP implants loaded with hFOB 1.19 cells formed osteoid, Collagraft loaded with hFOB 1.19 cells displayed a high degree of adipose tissue within the matrix. Immunostaining of serial sections for human nuclear antigen demonstrated that the osteoid contained human cells. Osteoid formation was not observed in control HA-TCP or Collagraft matrices implanted without cells. In summary, the data demonstrate the ability of ADAS cells to form osteoid matrix in vivo. Because of their abundance and accessibility, ADAS cells may prove to be a novel cell therapeutic for bone repair and regeneration.     

Effect of beta2-glycoprotein I null mutation on reproductive outcome and antiphospholipid antibody-mediated pregnancy pathology in mice

beta(2)-Glycoprotein I (beta(2)GPI) is a principal target antigen for antiphospholipid antibodies associated with recurrent pregnancy loss and fetal growth restriction in women. The significance of disrupted beta(2)GPI activity in contributing to pregnancy pathology in antiphospholipid syndrome (APS) is not clear. In this study the physiological requirement for functional beta(2)GPI in pregnancy was investigated by evaluating reproductive outcomes in beta(2)GPI null mutant (beta(2)GPI-/-) mice. beta(2)GPI-/- mice were fertile and carried viable fetuses to term. However, there was an 18% reduction in the number of viable implantation sites in beta(2)GPI-/- mice and reduced fetal weight and fetal:placental weight ratio in late gestation, suggesting compromised placental function. Placental architecture was altered in beta(2)GPI-/- implantation sites with a 24% increase in the junctional zone: labyrinthine ratio, but placentae showed no evidence of increased thrombosis in the absence of beta(2)GPI. The effect of beta(2)GPI genotype on pregnancy success after passive transfer of human and mouse antibodies reactive with beta(2)GPI was also explored. Two of five anti-beta(2)GPI antibodies induced pregnancy loss in beta(2)GPI+/+ mice but beta(2)GPI-/- mice were refractory to antibody-induced pregnancy failure. We conclude that functional beta(2)GPI is not essential for successful pregnancy in mice, but optimal placental development and fetal growth require this molecule. Together these data are consistent with pathogenic mechanisms in antiphospholipid syndrome involving both neutralization of beta(2)GPI function and beta(2)GPI-immunoglobulin complex formation.     

投入超脱量表的编制与信度效度检验

基于中国道家思想和认知治疗理论的道家认知疗法对个体认知的关注主要在其投入与超脱的程度。投入—超脱是一种对待人生的态度 ,一种价值观。它可以定义为与个体身心健康有关的对待生活的积极或消极的认知。由于生物的、心理的或社会的原因 ,人们对待生活的态度不同。有的人对待生活基本持投入的态度 ,表现为积极进取 ,希求成功 ;有的人基本持超脱的态度 ,表现为消极退守 ,宁静无为。就一般情形而言 ,无论个体的价值观怎样 ,只要是内心和谐而且社会适应良好 ,则应看作是健康的。投入的态度可能与较多的正性情感体验有关 ;超脱的态度可能与较…     

Plasmids encoding foot-and-mouth disease virus VP1 epitopes elicited immune responses in mice and swine and protected swine against viral infection

VP1 is a capsid protein of foot-and-mouth disease virus (FMDV) and contains epitopes of the virus. Plasmids encoding two VP1 epitopes (amino acid residues 141-160 and 200-213) and a host-self immunoglobulin molecule were constructed to produce a new type of FMD DNA vaccine. Two plasmids, namely, pCEIM and pCEIS, containing mouse immunoglobulin (IgG) or swine IgG were subjected to immunogenicity testing in mice and swine, respectively. In mice administrated pCEIM in the abdomen using a genegun, both FMDV-specific T-cell proliferation and neutralizing antibodies were detected. In swine immunized with pCEIS at the back of the ear, immune responses were achieved after the second administration. Swine showed a T-cell proliferative response with a stimulation index (SI) of up to 8.1 and a neutralizing antibody response that was able to protect suckling mice from 10(2) LD(50) (lethal dose 50) FMDV challenge. To compare the immunogenicity of the DNA-based vaccine candidate, versus the protein-based vaccine candidates, a second group of swine was immunized with the protein F1-scIgG, which was encoded by the plasmid pCEIS. Injection with F1-scIgG elicited a T-cell proliferative response of SI < 1.7 and a neutralizing antibody response that protected suckling mice from up to 10(5) LD(50) FMDV challenge. In the challenge test, three of three swine immunized with pCEIS were fully protected from FMDV challenge.     

男性不育患者Y染色体微缺失的研究

目的研究无精子症和少精子症患者与Y染色体位点缺失的相关性，建立Y染色体微缺失的分子诊断方法。方法采用多重PCR技术对53例染色体核型正常的无精子症和少精子症患者以及5例正常男性的无精子因子（azoospermia factor，AZF）区域的6个STS位点进行检测。结果5例精液正常男性未检出Y染色体微缺失；53例患者中6例有AZF区域的微缺失，总缺失率为11．3％。结论Y染色体微缺失是严重生精障碍的重要原因之一，无精子因子（AZF）候选基因在精子发生过程中可能起重要作用。