海风藤酮对小鼠抗着床作用的细胞化学和电镜观察

目的观察海风藤酮对小鼠的抗着床作用及其细胞化学和超微结构的变化。②方法向妊娠第２天小鼠子宫角内注射不同剂量的海风藤酮,于妊娠第８天和第１０天观察其对滋养层细胞的刀豆凝集素（ＣｏｎＡ）受体和麦胚凝集素（ＷＧＡ）受体表达,以及对滋养层细胞和子宫蜕膜细胞超微结构的影响。③结果随着海风藤酮剂量增大,滋养层细胞的ＷＧＡ和ＣｏｎＡ受体的阳性反应减弱,以ＷＧＡ受体的改变更明显。电镜所见,实验组绒毛浅层滋养层细胞胞浆电子密度低,核固缩;深层滋养层细胞溶酶体增多,见髓样小体,子宫蜕膜的大蜕膜细胞胞浆溶酶体增多,胞浆空泡化,核膜溶解。④结论海风藤酮可使小鼠胚胎滋养细胞的ＷＧＡ和ＣｏｎＡ受体糖蛋白减少,从而干扰了细胞之间的识别黏附,破坏了胚胎正常生长和发育的内环境,抑制着床过程。     

儿童偏头痛脑电图和经颅多普勒检查结果分析

本文对２５例儿童偏头痛患者进行发作期和间歇期脑电图及经颅多普勒检查。结果发现,脑电图异常率７２％,经颅多普勒异常率８５％,脑电图诱发试验６０％为阳性,其中３例脑电图在枕区可见单个非典型痫性波。提出儿童偏头痛的诊断应与癫痫鉴别,脑电图与经颅多普勒同时检查能提高诊断,对治疗有指导意义。     

PAI-2ΔCD突变体的构建及其在大肠杆菌中的表达与纯化

目的构建ＰＡＩ－２ΔＣＤ突变体基因,实现在大肠杆菌中的表达并建立重组人ＰＡＩ－２ΔＣＤ的分离纯化方法。方法用ＰＣＲ扩增ＰＡＩ－２ΔＣＤ基因,经限制性内切酶片段分析及核苷酸序列分析后,突变体基因与原核表达载体ｐＬＹ－４重组并转化蛋白酶缺陷型菌株ＪＦ１１２５。经温度诱导表达,表达产物用ＳＤＳ－ＰＡＧＥ,Ｗｅｓｔｅｒｎ印迹法和纤溶抑制活性测定等方法鉴定。工程菌发酵后,经高压匀浆破菌,用离子交换、疏水性色谱和分子筛进行分离。结果经温度诱导表达,ＳＤＳ－ＰＡＧＥ证实在相应相对分子质量处出现表达条带,约占全菌总蛋白的１５％。Ｗｅｓｔｅｒｎ印迹法证实表达产物具有ＰＡＩ－２免疫原性,溶圈抑制法及翻转板法证实表达产物具有纤溶抑制活性。５Ｌ发酵菌液可得湿菌约１００ｇ,经多步纯化后,每升发酵菌液可得纯度达９７％的ＰＡＩ－２ΔＣＤ约３６ｍｇ,比活性为２８６４０ＡＩＵ／ｍｇ。结论成功构建了ＰＡＩ－２ΔＣＤ突变体,实现了在大肠杆菌中的表达,并成功地分离纯化了重组人ＰＡＩ－２ΔＣＤ蛋白。     

中药壮骨胶囊治疗Ⅰ型原发性骨质疏松症74例临床研究

目的　探讨中药壮骨胶囊对Ⅰ型原发性骨质疏松症的疗效及其与骨代谢的关系。方法　采用益肾健脾的中药壮骨胶囊治疗７４ 例绝经后妇女原发性骨质疏松症,并与钙尔奇Ｄ 治疗的３１ 例作对照。结果　３ 个月后,其总有效率达９１ ．８９ ％ ,骨质疏松程度、血ＡＬＰ、ＣＴ、Ｅ２ 皆显著改善,且显著优于对照组（ Ｐ＜ ０．０５ ～０．０１）。无明显副反应。结论　绝经后妇女骨质疏松症主要由肾虚所致,采用中药补肾健脾治疗,疗效显著优于补钙、雌激素的替代疗法等。壮骨胶囊有调整骨代谢作用。该药安全可长期服用     

节段内固定治疗腰椎峡部裂的生物力学研究

在8具成人新鲜腰椎标本上,通过脊柱三维运动实验机,研究了横突棘突钢丝捆绑法、钩螺钉固定法及Buck螺钉固定法对峡部裂腰椎三维稳定性的影响。实验结果表明：三者均能显著地恢复脊柱的稳定性,且三者之间无显著差别。     

Apolipoprotein Serum Amyloid A in Alzheimer's Disease

Alzheimer's disease is characterized by the tissue deposition of beta-amyloid peptide (Abeta) in the brain. Recent studies have shown apoproteins (apo) in amyloid plaques and associated with high-density lipoprotein (HDL) particles in the cerebrospinal fluid (CSF). Western blot analysis revealed that serum amyloid A (apoSAA) protein was present in control and AD patients at low levels compared to apoE and apoA-I, however, AD brains showed a significant increase over control values. Analysis of CSF-HDL from control and AD individuals showed that apoA-I, apoE and apoSAA were on the particle. Immuno-cytochemical analysis showed that SAA was detected in senile plaques in AD tissue, but was predominantly localized to neuritic plaques. ApoE staining of AD brain confirmed that most plaques contained the apoprotein, similar to Abeta immunoreactivity, whereas apoA-I expressed little staining of senile plaques. No significant differences were detected in the level of apoSAA when compared to APOE genotype in AD samples, suggesting that interactions with apoE were non-specific. These data imply that the specific interactions of SAA with Abeta in the neuritic plaques may play a role in AD.     

Human leptomeningeal and cortical vascular anatomy of the cerebral cortex at 8 Tesla

PURPOSE: The purpose of this work was to describe the human leptomeningeal and cortical vascular anatomy as seen at high resolution on an 8 T UHFMRI system. METHOD: With a 1024 x 1024 matrix, axial gradient echo images of the cerebral cortex were acquired on a human volunteer at 8 T with TR 500 ms, TE 16 ms, flip angle 22.5 degrees, bandwidth 53 kHz, and slice thickness 2.84 mm. The same subject was evaluated at 1.5 T using similar parameters. The images were then reviewed in detail and compared with known cortical and leptomeningeal vascular anatomy. RESULTS: Two hundred forty micron in-plane resolution images of the human brain were acquired at 8 T without evident artifact from susceptibility distortions, RF penetration, or dielectric resonances. The CSF, gray matter, and white matter structures were well discerned. The microscopic leptomeningeal vascular anatomy was well visualized, and the course of small perforating cortical vessels could be followed from the cortical surface to the white matter junction. CONCLUSION: Initial 8 T images of the brain demonstrate detailed leptomeningeal and cortical vascular anatomy.     

Effect of estrogen on cellular metabolism of the human anterior cruciate ligament

Previous studies from this laboratory have established the presence of estrogen receptors in the human anterior cruciate ligament. The purpose of this study was to investigate the effects of 17 beta-estradiol on cell proliferation and procollagen levels, as an indicator of collagen synthesis, in the human anterior cruciate ligament fibroblasts. Fibroblast proliferation and procollagen synthesis in response to near log concentrations of 17 beta-estradiol (at 0.0029 ng/mL, 0.025 ng/mL, 0.25 ng/mL, 2.5 ng/mL, and 25 ng/mL) were assessed with the measurement of 3H-thymidine incorporation and Types 1 and 3 procollagen specific equilibrium radioimmunoassays. On Days 1 and 3, there was a dose dependent decrease in the proliferation of anterior cruciate ligament fibroblasts with increasing estradiol concentrations. This dose dependent effect of decreased fibroblast proliferation with increasing estradiol concentrations became less apparent at 7, 10, and 14 days. On Days 1 and 3, procollagen synthesis decreased in a dose dependent manner with increasing estradiol concentrations. On Days 7, 10, and 14, this dose dependent effect was attenuated. No significant differences in Type 3 procollagen synthesis by anterior cruciate ligament fibroblasts were observed with varying estradiol concentrations at any of the designated points. These early physiologic changes in fibroblast proliferation and Type I procollagen synthesis may provide a biologic explanation for the increased anterior cruciate ligament injury rate observed in female athletes, suggesting that it is the acute cyclic variations in the female athlete who is menstruating that predisposes her to ligamentous injury.