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81.
Propensity score‐matched study of laparoscopic and open surgery for colorectal cancer in rural hospitals 下载免费PDF全文
Toshihiro Nakao Mitsuo Shimada Kozo Yoshikawa Jun Higashijima Takuya Tokunaga Masaaki Nishi Chie Takasu Hideya Kashihara Ichio Suzuka Takashi Nishizaki Hiroshi Okitsu Toshiyuki Yagi Hidenori Miyake Murato Miura Mitsutoshi Fukuyama Daisuke Wada Yoshiaki Bando 《Journal of gastroenterology and hepatology》2016,31(10):1700-1704
82.
83.
Yasuyuki Miyake Yasushi Okoshi Takayuki Machino Shigeru Chiba 《International journal of hematology》2010,92(3):474-480
B cell lymphomas often develop in the central nervous system (CNS). Although rituximab (RTX) has been widely used for most
B cell lymphomas, the efficacy for CNS lymphomas has yet to be elucidated. A major concern is that RTX might not reach lymphoma
lesions, and either the antibody-dependent cellular cytotoxicity or complement-dependent cytotoxicity might not substantially
operate in the CNS environment. Here we investigated the potential usefulness of co-administering RTX and human serum intraventricularly
in nude rats carrying human B cell lymphomas in the CNS. Raji, a CD20-positive lymphoma cell line, was inoculated into the
cerebrum of F344 (rnu/rnu) nude rats. After several days, RTX and human serum were delivered into the ipsilateral lateral ventricle via a cannula.
Intraventricularly administered RTX was localized specifically at the lymphoma lesions, indicating that RTX penetrated the
ependymal layer of the lateral ventricle to reach the tumor lesion, where it specifically bound to the lymphoma cells. The
combination of RTX and serum (n = 12), but not RTX alone (n = 13), significantly extended the survival of the rats (P = 0.049). Intraventricular administration of RTX and serum in a rat/human CNS lymphoma model might be a potential novel treatment
for CNS lymphomas of B cell origin. Clinical trials are warranted. 相似文献
84.
Tokunori Mukai Yuji Hiromatsu Michiko Ichimura Tomoka Fukutani Hiroo Kaku Ikuyo Miyake Shingo Shoji Yoshiro Koda Tomasz Bednarczuk 《Thyroid》2006,16(3):243-248
OBJECTIVE: To investigate whether polymorphisms of interleukin (IL)-18 gene confer susceptibility to Graves' disease (GD) and Graves' ophthalmopathy (GO). DESIGN: We performed a case control study on polymorphisms of IL-18 gene in Japanese patients with GD (n = 435), and healthy control subjects without antithyroid autoantibodies or family history of autoimmune disorders (n = 255). The C-4675G, C-607A, and G-137C polymorphisms in the promoter region and A105C (exon 5) polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using restriction enzymes, sequence-specific PCR, and PCR-direct sequencing methods. RESULTS: None of the polymorphisms in the IL-18 gene were associated with development of Graves' disease. The CC genotype and C allele frequencies of IL-18 gene G-137C polymorphism tended to be greater in patients with ophthalmopathy than in patients without evident ophthalmopathy. However, the differences were not statistically significant. Although there were three major haplotypes, none of the haplotypes were statistically associated with susceptibility to GD or ophthalmopathy. CONCLUSIONS: These results suggest that IL-18 gene polymorphisms are not major genetic factors for susceptibility to GD in a Japanese population. Further studies with adequate sized data set in the subset analyses for GO are needed. 相似文献
85.
Yoko Hiraki Satoko Miyatake Michiko Hayashidani Yutaka Nishimura Hiroo Matsuura Masahiro Kamada Takuji Kawagoe Keiji Yunoki Nobuhiko Okamoto Hiroko Yofune Mitsuko Nakashima Yoshinori Tsurusaki Hirotomo Satisu Akira Murakami Noriko Miyake Gen Nishimura Naomichi Matsumoto 《American journal of medical genetics. Part A》2014,164(1):231-236
86.
This study concerns the immunohistochemical localization of S-100 alpha, S-100 beta, and whole brain S-100 (wbS-100) in testicular large-cell calcifying Sertoli cell tumor (LCCSCT). We examined 8 LCCSCTs (7 benign and 1 malignant), 6 Sertoli cell tumors not otherwise specified (SCTs-NOS), 6 Leydig cell tumors (LCTs), 5 ovarian Sertoli-Leydig cell tumors (SLCTs), and 7 gonadoblastomas (GBLs). The 8 LCCSCTs showed immunoreactivity for S-100 alpha, S-100 beta, and wbS-100. Five of the 6 LCTs and the Leydig cell components in the ovarian SLCTs stained positively for S-100 alpha and wbS-100 but were negative for S-100 beta. SCTs-NOS and the Sertoli cell components in the SLCTs occasionally showed focal and weak/moderate positivity for S-100 alpha, S-100 beta, and wbS-100. Sex cord cells of the GBLs were positive for S-100 beta and wbS-100 and negative for S-100 alpha. Germ cell elements of the GBLs were negative for S-100 alpha, S-100 beta, and wbS-100. In nonneoplastic testicular parenchyma adjacent to the above-mentioned tumors, there was S-100 alpha reactivity in Leydig cells, rete testis, and a few Sertoli cells. S-100 beta reactivity was seen in a few Sertoli cells, Schwann cells, and some endothelial cells. WbS-100 reactivity was present in Leydig cells, a few Sertoli cells, rete testis, Schwann cells, and some endothelial cells. The results indicate that S-100 alpha and S-100 beta can potentially be used as immunohistochemical markers for LCCSCT, especially when differentiating it from LCT, which may mimic LCCSCT on routine histopathology. Although the biological significance of both S-100 subunits expression in LCCSCT remains unknown, these notable calcium-binding proteins may be associated with the characteristic calcification in LCCSCT through regulation of calcium levels in the tumor cells. 相似文献
87.
Case of Mycobacterium haemophilum misdiagnosed as Mycobacterium intracellulare due to one base insertion in the bacterial genome 下载免费PDF全文
Rika Nishikawa Yozo Yamada Haruhisa Kanki Hiroshi Matsuoka Tatsuya Nakamura Takumi Jikimoto Mari Kusuki Norihisa Ishii Kenichiro Ohnuma Kazue Nakanaga Chikako Nishigori 《The Journal of dermatology》2018,45(1):64-66
Mycobacterium haemophilum is a slow‐growing, non‐tuberculous mycobacteria that causes cutaneous infection. We describe a case of cutaneous infection in a 68‐year‐old Japanese man with polymyositis. This was caused by M. haemophilum harboring one base insertion in gene sequence. At first, the causal microorganism was misidentified as M. intracellulare by COBAS® TaqMan® MAI test. However, poor growth on Ogawa media and growth enhancement on 7H11C agar around a hemin‐containing disk prompted us to reinvestigate the causal microorganisms, which were revealed to be M. haemophilum. Amplified polymerase chain reaction products were sequenced, and the 16S rRNA gene, rpoB, hsp65 and internal transcribed spacer region sequences showed a 100%, 100%, 99.66% and 99.7% match, respectively, with the corresponding regions of M. haemophilum, but it harbored a novel gene sequence in hsp65. The sequences determined by gene analysis of the M. haemophilum strain were deposited into the International Nucleotide Sequence Database. Although numerous cases of M. haemophilum infection have been reported in other countries, only six cases have been reported in Japan to date. It could be possible that this novel mutation lead to misdiagnosis. As M. haemophilum prefers a lower growth temperature (30–32°C) and it requires iron in the culture medium, M. haemophilum could be misidentified or overlooked. Accordingly, a M. haemophilum infection should be considered in cases of cutaneous infection of the body sites, of which surface temperature is low. 相似文献
88.
The Molecular Mechanism of B Cell Activation by toll-like
Receptor Protein RP-105 总被引:6,自引:1,他引:6 下载免费PDF全文
Vivien W.F. Chan Ingrid Mecklenbruker I-hsin Su Gemma Texido Michael Leitges Rita Carsetti Clifford A. Lowell Klaus Rajewsky Kensuke Miyake Alexander Tarakhovsky 《The Journal of experimental medicine》1998,188(1):93-101
The B cell–specific transmembrane protein RP-105 belongs to the family of Drosophila toll-like proteins which are likely to trigger innate immune responses in mice and man. Here we demonstrate that the Src-family protein tyrosine kinase Lyn, protein kinase C β I/II (PKCβI/II), and Erk2-specific mitogen-activated protein (MAP) kinase kinase (MEK) are essential and probably functionally connected elements of the RP-105–mediated signaling cascade in B cells. We also find that negative regulation of RP-105–mediated activation of MAP kinases by membrane immunoglobulin may account for the phenomenon of antigen receptor–mediated arrest of RP-105–mediated B cell proliferation. 相似文献
89.
Teruki Miyake Bunzo Matsuura Shinya Furukawa Toru Ishihara Osamu Yoshida Masumi Miyazaki Kyoko Watanebe Akihito Shiomi Hironobu Nakaguchi Yasunori Yamamoto Yohei Koizumi Yoshio Tokumoto Masashi Hirooka Eiji Takeshita Teru Kumagi Masanori Abe Yoshio Ikeda Takeru Iwata Yoichi Hiasa 《Journal of diabetes investigation.》2022,13(7):1245
IntroductionNonalcoholic fatty liver disease (NAFLD) is diagnosed after excluding other liver diseases. The pathogenesis of NAFLD when complicated by other liver diseases has not been established completely. Metabolic dysfunction‐associated fatty liver disease (MAFLD) involves more metabolic factors than NAFLD, regardless of complications with other diseases. This study aimed to clarify the effects of fatty liver occurring with metabolic disorders, such as MAFLD without diabetes mellitus (DM), on the development of DM.Materials and MethodsWe retrospectively assessed 9,459 participants who underwent two or more annual health check‐ups. The participants were divided into the MAFLD group (fatty liver disease with overweight/obesity or non‐overweight/obesity complicated by metabolic disorders), simple fatty liver group (fatty liver disease other than MAFLD group), metabolic disorder group (metabolic disorder without fatty liver disease), and normal group (all other participants).ResultsThe DM onset rates in the normal, simple fatty liver, metabolic disorder, and MAFLD groups were 0.51, 1.85, 2.52, and 7.36%, respectively. In the multivariate analysis, the MAFLD group showed a significantly higher risk of DM onset compared with other three groups (P < 0.01). Additionally, the risk of DM onset was significantly increased in fatty liver disease with overweight/obesity or pre‐diabetes (P < 0.01).ConclusionsFatty liver with metabolic disorders, such as MAFLD, can be used to identify patients with fatty liver disease who are at high risk of developing DM. Additionally, patients with fatty liver disease complicated with overweight/obesity or prediabetes are at an increased risk of DM onset and should receive more attention. 相似文献
90.
Synthesis and secretion of human epidermal growth factor by Escherichia coli. 总被引:12,自引:5,他引:12 下载免费PDF全文
T Oka S Sakamoto K Miyoshi T Fuwa K Yoda M Yamasaki G Tamura T Miyake 《Proceedings of the National Academy of Sciences of the United States of America》1985,82(21):7212-7216
A synthetic gene for human epidermal growth factor (hEGF) was joined to a sequence encoding the signal peptide of Escherichia coli alkaline phosphatase. This hybrid gene was placed under the control of the alkaline phosphatase gene (phoA) promoter in a recombinant plasmid, which was used to transfect E. coli. The hybrid protein that was expressed in host cells under conditions of phosphate limitation was processed accurately during the secretion process, and mature hEGF was recovered in the periplasmic fraction. On the other hand, no EGF was detected in the periplasmic space when the synthetic hEGF gene was not accompanied by the phoA signal sequence. 相似文献