The remucosalizing alar cartilage flap: a reconstructive option for repairing nasal septal perforations

The objective of this study is to assess the results of repairing septal perforations with a vascularized pedicled alar cartilage island flap. Using the external rhinoplasty approach, a vascularized flap of alar cartilage, harvested as a cephalic trim and pedicled on the ascending columellar branches of the superior labial artery was raised. Bilateral mucoperichondrial septal flaps were elevated and the alar flap was transposed and secured within the defect and bilaterally overlaid with temporalis fascia. Silastic sheets were placed and remained in situ until the grafts were revascularized from the peripheries of the defect as well as centrally from the alar flap. The revascularized temporalis fascia acted as a scaffold for nasal remucosalization. The alar flap also increased the long-term structural robustness of the repair. Between 1999 and 2003, 14 patients with septal perforations ranging from 10 to 31 mm underwent septal reconstruction using this technique. There were nine males and five females. The flap was successfully raised in all cases and long-term closure was maintained in 12 patients (86%). The alar cartilage flap is an effective technique for repairing septal perforations in selected patients. It provides vascularized tissue which nourishes the grafts during remucosalization, and a cartilaginous framework, which affords long-term structural support to the repair. It also obviates the need to transpose nasal mucosa and create a secondary defect. The rhinoplasty approach furthermore permits additional nasal deformities to be corrected at the same time. Presented at the British Association of Plastic Surgeons Summer Scientific Meeting, Sheffield, UK (12 July 2006).     

神经肽Y对心肌蛋白质合成的影响及其意义

用~3H-亮氨酸参入法与放射免疫分析法分别测定大鼠心肌蛋白质合成速度与心脏组织中神经肽Y(NPY)含量。结果显示NPY能显著促进心肌蛋白质合成,其最大效应较去甲肾上腺素(NE)大35%,所需浓度是NE的1/100。心肌梗塞后非梗塞区组织内NPY的含量在2天时无显著改变,15天时则降低71%,提示2～15天期间该区域NPY释放增加,此为非梗塞区心肌肥大的机制之一     

Surface activation markers of T lymphocytes: role in the detection of infection in neonates

Diagnosis of perinatal infection in the newborn is difficult; there may be few clinical signs and current tests are slow or non-specific. Detection of organisms, antigen or specific antibody to common pathogens often requires repeat samples and does not give immediate results. Haematological parameters, although relied upon frequently to diagnose infection in the neonate prior to a positive bacterial isolation, are unreliable and insensitive. Indicators such as an increase in neutrophil band cell counts are highly variable between morphologists. Infection induces the expression of a number of T lymphocyte surface markers, including CD45RA/CD45RO and CD45RO. The use of changed expression of surface markers as a laboratory test for detection of infection in neonates was evaluated. We used multiparameter flow cytometry to detect expression of early (CD45RA/CD45RO) and late (CD45RO) activation markers. In the respective groups of 50 full term (including 25 normal vaginal deliveries and 25 caesarean deliveries) and 30 premature, i.e. < 36 weeks gestation (born by either normal vaginal delivery or caesarean delivery) the CD45RA isoform was brightly expressed on newborn ‘naive’ CD4⁺ T cells, whereas the CD45RO isoform (including both ‘bright’ and ‘dim’ populations) was present on < 19% of CD4⁺ T cells from these newborn infants. In a group of 37 infants, tested to evaluate possible effects of non-infective parameters such as respiratory distress and iso-immunization, no significant changes in surface marker expression were found and specificity of the test was confirmed. In 14 neonates with documented sepsis, up-regulation of dual staining CD45RA/CD45RO isoforms on CD4⁺ T cells was detected early in the infection. In addition, we found that CD45RO expression persisted for several weeks after bacterial infection, and up to several months in viral infection. In conclusion, detection of T cell activation by flow cytometry for the early diagnosis of neonatal infection is an easy test to carry out on small volumes of blood, is inexpensive, and may be a specific indicator of infection.     

应用门控SPECT测量左心室功能的方法学研究

目的：试用门控心肌显像测定左室功能并与心血池显像结果比较。材料和方法：61例受检者进行了Tc-99m—MIBI门控心肌显像及心血池显像．结果：门控断层心肌显像的短轴中间部左室功能结果与平衡法心血池显像有良好的相关性(r=0．82）。结论：门控心肌断层显像测定的左室功能结果方法简便可靠．能同时反映心肌直流及左室功能。     

The retinae of Prototherian mammals possess neuronal types that are characteristic of non-mammalian retinae

This study has shown that the retinae of Prototherian (egg-laying) mammals possess two neuronal types that are present in non-mammalian retinae, but absent or morphologically different in the retinae of Eutherian (placental) mammals. First, endogenous serotonin-like immunoreactivity has been localized in a population of presumptive amacrine cells in the platypus retina, the first such report in a mammalian retina. Second, the protein kinase C-immunoreactive (PKC-IR) bipolar cells in the echidna retina appear similar to the PKC-IR bipolars in the chicken retina, in that their dendrites give rise to a Landolt's club and their axons are multistratified. By contrast, the PKC-IR rod bipolar cells in the rabbit and in the brushtail possum, a Metatherian (marsupial) mammal, have no Landolt's clubs and their axons form terminal lobes in the innermost stratum of the inner plexiform layer.     

大鼠前脑和脑干向中央上核的纤维投射

用辣根过氧化物酶(HRP)微电泳技术研究大鼠前脑和脑干向中央上核(CS)的纤维投射。将HRP输入CS后,观察到缰核、脚间核和被盖背核区有较多的标记细胞,斜角带核、下丘脑核和乳头体核有少量标记细胞。在外侧缰核、脚间核和被盖背核区可见丰富的顺行标记终末。结果表明,前脑的缰核和脑干的脚间核和被盖背核区是CS的主要传入来源,它们之间存在往返的纤维联系。     

谷参肠安胶囊长期毒性试验

本文报告谷参肠安胶囊大鼠长期毒性试验,结果表明,谷参肠安胶囊对动物的一般状况,体重增长、外周血象、肝肾功能及病理组织学检查等未见明显毒性。