静脉滴注尼莫地平治疗急性缺血性脑梗死

发病72h内的急性缺血性脑梗死60例,分为2组。尼莫地平组30例(男性21例,女性9例;年龄59±s10a)。wk1.2给支持疗法(脱水剂,维生素C等)和尼莫地平2mg/d于5％葡萄糖液500ml内静脉滴注。wk3.4改用扩容、改善微循环,细胞活性药。wkl-4口服尼莫地平60mg.qn。对照组30例(男性23例,女性7例;年龄58±9a)。不给尼莫地平,其余同上。4wk后,前组神经功能缺损积分值较后组下降显著(P相似文献   

三结构域重组纤连蛋白多肽在大肠杆菌中表达及其产物的纯化与鉴定

在大肠杆菌中表达了一种抗肿瘤转移多肽——人纤连蛋白（ＦＮ） 的ＣｅｌｌＩ－Ｈｅｐ Ⅱ－ＣｅｌｌⅡ三结构域重组多肽（ＣＨ８２）, 表达效率达２１％ 。在低温 （２２ ℃） 培养表达时, ＣＨ８２大部分为可溶性产物, 经ＤＥＡＥ－５２ 层析及Ｈｅｐａｒｉｎ－ａｇａｒｏｓｅ亲和层析可得到纯品; 在高温（３７ ℃） 培养表达时, ＣＨ８２ 主要以包涵体形式出现, 经尿素变性与复性处理后,可通过Ｈｅｐａｒｉｎ－ａｇａｒｏｓｅ 亲和层析得到纯品。所得纯品均具有结合肝素和结合细胞的功能, 且结合细胞的能力比双结构域ＦＮ更强, 表明两个结合细胞的功能结构域均有活性     

人型结核杆菌克隆DNA探针的制备及其应用的研究

用限制性内切酶ＰｓｔⅠ消化人型结核杆菌Ｈ３７ＲｖＤＮＡ,与质粒ｐＵＣ１９ ＤＮＡ连接后转化大肠杆菌,经同位素３２Ｐ标记的人型结核杆菌Ｈ３７Ｒｖ全染色体ＤＮＡ探针筛选出６个阳性克隆（ＭＴ１￣６）,其中克隆ＤＮＡ片段ＭＴ２（４．２Ｋｂ）、ＭＴ４（３．５Ｋｂ）和ＭＴ５（３．０Ｋｂ）标记成探针,检测了２２种分支杆菌标准株和１５侏人型结核杆菌临床分离侏以及２种非分支杆菌,其杂交结果完全相同,与人型结核杆菌及     

80例正常人体表标测Q-T_d~p的观察

采用９１ 导联体表电位标测法（ Ｂ Ｓ Ｐ Ｍ） 观察８０ 例正常人 Ｑ Ｔｐｄ（ Ｑ 波起点到 Ｔ 波峰值间期的离散度） ,与同步１２ 导联心电图 Ｑ Ｔ 离散度（ Ｑ Ｔｄ） 对照,探讨 Ｂ Ｓ Ｐ Ｍ 条件下测定 Ｑ Ｔｄ 的可行性。结果：① Ｑ Ｔｐ 、 Ｑ Ｔｐｄ 、 Ｑ Ｔｐｃ１ｄ（ Ｂａｚｚｅｔｔ 公式校正） 、 Ｑ Ｔｐｃ２ｄ（ Ｆｒｉｄｅｒｃｉａ 公式校正） 分别为（２９２ ．６９ ±３７ ．７５） ｍｓ 、（３６ ．７７ ±７ ．４０） ｍｓ 、（４０ ．２３ ±９ ．０４） ｍｓ 和（４０ ．１１ ±７ ．７３） ｍｓ ;② Ｑ Ｔｐｄ 与 Ｑ Ｔｄ 、 Ｑ Ｔｐｃ１ｄ 与 Ｑ Ｔｃ１ｄ 、 Ｑ Ｔｐｃ２ｄ 与 Ｑ Ｔｃ２ｄ 有良好的相关性;③３ 个年龄组（１８ ～３５ 岁、３６ ～６０ 岁、６１ ～７０ 岁） 各参数间无显著性差异;④ Ｑ Ｔｐ 女性明显大于男性（ Ｐ＜ ０ ．０５） ,其余各值男女性之间均无显著性差异。提示： Ｂ Ｓ Ｐ Ｍ 测定 Ｑ Ｔｐｄ 简便可行、较为准确,正常值可暂定为５２ ｍｓ 。     

关于医疗卫生单位公有制实现形式多样化的探讨

医疗卫生单位公有制实现形式的多样化十分必要：①发展医疗卫生事业不能单靠政府的投入;②人民群众对医疗卫生服务呈现出多层次的需求;③卫生产业的发展呼唤医疗卫生单位公有制实现形式多样化。实现医疗卫生单位公有制形式多样化的基本条件已具备：①国家宏观经济方针的两个根本性转变使实现医疗卫生单位公有制形式多样化成为可能;②医疗卫生行业业已确立的公有制优势地位有利于实现公有制形式的多样化;③卫生产业的发展已为实现公有制形式的多样化积累了成功的经验。医疗卫生单位公有制形式多样化的实现策略：①大型公有医疗卫生单位的经营多元化;②中小公有医疗卫生单位的股份制改造;③社会筹资和财团法人办医;④医疗卫生行业适度对外开放;⑤允许民营医疗卫生单位有所发展     

计算机2000年问题对医院的影响及对策

计算机２０００年问题对医院最明显的影响是计算机系统届时交病的信息丢失,财务系统不能正常工作,网络瘫痪等。此外,由于嵌入式问题将造成医疗设备运行混乱,从而危及病人的生命和健康。医院应采取的对策是：认知计算机２０００年问题,产品清查,确定问题范围,对问题进行评估,制定应急计划,制定解决方案,具有体实施和检验验证。     

锥兰联合茜素红活细胞染色法观察角膜中央碱烧伤内皮细胞愈合过程

目的:研究角膜碱烧伤内皮细胞的愈合过程。方法:锥兰联合茜素红内皮细胞染色法对角膜中央碱烧伤内皮细胞形态学变化进行观察。结果:伤后20分钟内皮细胞已破坏。72小时,损伤区的周边内皮细胞变形向创面内迁移。第7天后缺损区大部分范围均可见梭形细胞覆盖。结论:兔碱烧伤损伤区内皮细胞的修复由邻近内皮细胞变形,增殖和移行长入创面内完成,修复的内皮细胞具有纤维细胞特征。眼科学报1999;15:218-220。     

An Experimental Study on Homoharringtonine Liposome and Glaucoma Filtration Surgery

Purpose: To observe the inhibitory action of homoharringtonine liposome during the healing process of wounds in the filtering sitesMethods: posterior sclerectomies were performed in 14 rabbits. Postoperatively one eye of each rabbit received subconjunctival injections of HH liposome and fellow eye received saline injection in a randomized masked fashion.Results; Fourteen days after operation the IOP of experimental eyes reduced significantly (P < 0.01) as compared with the controlled eyes, and the number of remaining filtering blebs increased noticeably (P< 0.05). Pathohistological examination revealed that the number of fibroblasts per square micron in the filtering sites and the thickness of the scars in the center of the filtering sites of the experimental eyes were less than those of the controlled eyes. No serious ocular toxic and side effects were found.Conclusion : This experiment suggests that homoharringtonine liposome can markedly inhibit the scar formation of filtering sites after glaucoma fi     

Effect of Liposome—Encapsulated Total Alkaloid of Harmaline on Rabbit Lens Epithelial Cells：Experimental Study on the Prevention of Posterior Capsule Opacification

Purpose: To investigate whether liposome encapsulated total alkaloid of Harmaline (TAH) as a therapeutic agent is beneficial to prevention of posterior capsular opacifi-cation (PCO).Methods: Liposome-encapsulated TAH was prepared by modified freeze-thawing method. 0. 1ml of liposome-encapsulated TAH (0. 2mg/ml) was injected into the capsular bag during extracapsular lens extraction (ECLE) of each eye in total 10 rabbit eyes. Blank liposome or balance salt solution (BSS) was used as control. Slit-lamp examination and histopathological examination was used to evaluated capsule opacifica-tion. Intraocular pressure (IOP) , density and morphology of corneal endothelia cells, the amplitude and latency of b wave of ERG were measured.Results: The inflammatory response was mild both in TAH treated and the control group. PCO formation occurred in the control group 2 weeks postoperatively, but the posterior capsule was clear in TAH treated eyes. 4 weeks and 8 weeks after operation, PCO occurred both in TAH treated     

A Study on the Compliance of the Patients with Ocular Fundus Diseases

Purpose: To investigate the compliance of the patients with ocular fundus diseases with recommendation for follow-up examination after laser treatment, and the underlying reasons for non-compliance.Methods: 53 patients with ocular fundus disease were asked to fill in a questionnaire which includes the socio-demographic characteristics, fear of the fundus disease, conception of the laser treatment and the motivation for staying healthy. Variables were compared for the compliers group and the non-compliers group by chi-square test. Result; Of the 53 subjects, 35 were classified as non-compliers and 18 were classified as compliers. There was no statistically significant difference between the two groups on all selected socio-demographic factors, conception of the laser treatment and the motivation for staying healthy. Significant difference was found between the two groups on two of those items concerning the fear of the diseases (P<0. 05). About half of the participants showed the lack of knowledge about