带有蛋白转导结构域的bcr/abl癌蛋白片段表达及其跨膜转运

0　引言　人类免疫缺陷病毒 (human immunodeficiencyvirus,HIV) - 1编码的反式激活蛋白 TAT具有独特的跨膜运转方式 ,而且有转导速度快 ,效率高的特点 ,被称为蛋白转导结构域 (protein transduction domain,PTD) [1 ,2 ] .本研究用PCR扩增了慢性粒细胞白血病慢粒 bcr/ abl融合蛋白的基因片段 ,在其 5′端融合 PTD结构域的编码区后在大肠杆菌中进行了表达 .表达产物经纯化后 ,加入培养的 HL 6 0细胞 ,表达的蛋白可直接进入细胞内 .这一结果为用外源蛋白负载(L oading)免疫细胞提供了新的途径 .1　材料和方法1.1　 DNA重组　人工合…     

面肌痉挛显微血管减压术的诱发肌电图监测和评估

目的:探讨面神经诱发肌电图在显微血管减压(MVD)术中、术后对面肌痉挛治疗效果的监测和评估。方法:对26例典型面肌痉挛病人、探讨在MVD术前、术中、术后,经皮刺激痉挛侧(手术侧)面神经下颌缘支,记录诱发同侧眼轮匝肌肌电位(MD-OC反应)变化,并以正常侧作对照检查。结果:26例术前均记录到痉挛侧MD-OC反应,在术中操作不同阶段,21例均见MD-OC反应消失。术后1周～3个月随访复查21例中,12例痉挛完全消失,MD-OC反应不复存在,9例症状明显或部分减轻,但有4例再记录到MD-OC反应。另5例术中,术后均记录到MD-OC反应,症状未有改善。结论:面神经诱发肌电图运用于MVD术中监测和预后判断,可改善和提高面肌痉挛的治愈率。     

药铜宫内节育器的恒河猴实验研究

实验选用恒河猴放置药铜宫内节育器（ＬＮＧ－Ｃｕ－ＩＵＤ：ＬＮＧ６μｇ／ｄ，Ｃｕ２００ｍｍ２）６个月，以观察其节育效果及月经情况。结果：节育效果好，无妊娠、无脱落；月经改变表现为出血不规则、经量减少及闭经，但取器后即恢复正常；宫腔冲洗液ＰＧｓ放免测定，置器前后ＰＧＥ２、ＰＧＦ２α、６－ｋｅｔｏ－ＰＧＦ１α浓度无显著差异，ＴＸＢ２明显上升，６－ｋｅｔｏ－ＰＧＦ１α／ＴＸＢ２比值下降，提示ＬＮＧ－Ｃｕ－ＩＵＤ导致周期出血减少可能与此有关；置器后子宫内膜上皮细胞和若干酶活性不同程度受抑。结果提示：兼释ＬＮＧ和Ｃｕ２＋的ＩＵＤ具有节育效果可靠、经血量少等优点，但对月经的影响尚需深入研究。     

A new staging system for hepatocellular carcinoma associated with portal vein tumor thrombus

BackgroundA new staging system for patients with hepatocellular carcinoma (HCC) associated with portal vein tumor thrombus (PVTT) was developed by incorporating the good points of the BCLC classification of HCC, and by improving on the currently existing classifications of HCC associated with PVTT.MethodsUnivariate and multivariate analysis with Wald χ² test were used to determinate the clinical prognostic factors for overall survival (OS) in patients with HCC and PVTT in the training cohort. Then the conditional inference trees analysis was applied to establish a new staging system.ResultsA training cohort of 2,179 patients from the Eastern Hepatobiliary Surgery Hospital and a validation cohort of 1,550 patients from four major liver centers in China were enrolled into establishing and validating a new staging system. The system was established by incorporating liver function, general health status, tumor resectability, extrahepatic metastasis and extent of PVTT. This staging system had a good discriminatory ability to separate patients into different stages and substages. The median OS for the two cohorts were 57.1 (37.2–76.9), 12.1 (11.0–13.2), 5.7 (5.1–6.2), 4.0 (3.3–4.6) and 2.5 (1.7–3.3) months for the stages 0 to IV, respectively (P<0.001) in the training cohort. The corresponding figures for the validation cohort were 6.4 (4.9–7.9), 2.8 (1.3–4.4), 10.8 (9.3–12.4), and 1.5 (1.3–1.7) months for the stages II to IV, respectively (P<0.001). The mean survival for stage 0 to 1 were 37.6 (35.9–39.2) and 30.4 (27.4–33.4), respectively (P<0.001).ConclusionsA new staging system was established which provided a good discriminatory ability to separate patients into different stages and substages after treatment. It can be used to supplement the other HCC staging systems.     

Lead-induced cell signaling cascades in GT1-7 cells

The effects of lead on the signal transduction pathways that may be involved in the release of gonadotropin-releasing hormone (GnRH) from neurons in the hypothalamus have not been well defined. Using the GT1-7 cell line, an in vitro model for GnRH-secreting neurons, we examined signal transduction pathways directly affected by lead. We found that lead-induced phosphorylation of extracellular signal-regulated kinase 1 and 2 (ERK1 and ERK2), as well as p90RSK and cAMP response element-binding protein (CREB), but did not induce IkappaB degradation. MEK1/2 inhibitor (PD98059) suppressed lead-induced ERK and p90RSK activation. Neither PKC inhibitors (Go6983, Go6976) nor CaMKII inhibitor (KN-62) had a pronounced effect on lead-induced ERK1 and ERK2 phosphorylation. However, MEK1/2 inhibitor, CaMKII inhibitor, and PKC inhibitor significantly suppressed lead-induced CREB phosphorylation. These results indicate that lead-activated PKC, CaMKII and MEK/ERK/p90RSK pathways simultaneously, all of which contributed to CREB phosphorylation. Our results also indicate that lead-induced p90RSK and CREB activation does not alter expression of early response genes like c-fos. We conclude that lead activates PKC, CaMKII or MEK-ERK-p90RSK pathways in GT1-7 cells, leading to CREB phosphorylation and modulation of gene expression.     

人胃癌双功能抗体制备的研究

以不同技术路线制备Ｐ２１０／ＭＭＣ，Ｐ２１０／ＴＮＰ及Ｐ４０／ＣＤ３三种双功能抗体（ＢｓＡｂ）。在１１次细胞融合中，接种７４８４孔，获１５株二次杂交瘤，其中５株分泌ＢｓＡｂ。将亲本杂交瘤分别诱变为ＨＧＰＲＴ＾－及ＴＫ＾－，融合后以ＨＡＴ选择培养并辅以饲养细胞较易获得二次杂交瘤。证明两种抗原交互反应的桥联试验，特别是当二次杂交瘤分泌不同类别的Ｉｇ时，对于确证ＢｓＡｂ的存在有着重要意义。本文首次报告，     

单纯性肥胖儿童自我意识水平、社会适应能力与行为问题研究

本文采用 Piers-Harris 儿童自我意识量表与 Achenbach 儿童行为调查表对50名7—11岁单纯性肥胖儿童及50名正常对照儿童的自我意识水平、社会适应能力和行为问题进行了研究,结果表明单纯性肥胖儿童有自我意识受损,自我评价差,突出表现为幸福与满足感差。随着肥胖程度的加重,男性儿童内向性、抑郁性及分裂样行为问题增多,社会适应能力降低,提示对单纯性肥胖儿童有必要进行心理干预。     

大鼠缺血心肌线粒体损伤及其意义

选用Wistar大鼠皮下注射异丙肾上腺素（ISP，5mg/kg)造成心肌缺血损伤模型。结果证实缺血心肌线粒体膜有明显损伤，表现在线粒体膜磷脂含量降低，胆固醇含量及胆固醇与磷脂比值增大，Ca^2 浓度显著增加，这些变化与氧自由基引发的脂质过氧化作用增强相关。     

学龄前听障儿童助听后言语流畅性的特征及影响因素

目的 分析学龄前听障儿童助听后言语流畅性的特征,探讨辅听装置、年龄、性别、语训时长对其言语流畅性的影响.方法 以109例3.5~6.5岁学龄前健听及听障儿童为研究对象,其中健听儿童30例(健听组),双耳佩戴助听器的听障儿童28例(助听器组),右耳植入人工耳蜗的听障儿童26例(人工耳蜗组),同时佩戴助听器和人工耳蜗双耳双模式助听的听障儿童25例(双模式组),通过主题对话的形式分别采集各组儿童的语音样本,比较各组儿童在自发性言语语言任务下的语速、停顿、重复和拖延差异;分析辅听装置、年龄、性别、语训时长等因素对听障儿童言语流畅性的影响.结果 ①健听组儿童的语速显著高于其他三组(P<0.05),健听组停顿次数显著低于人工耳蜗组(P=0.001)和双模式组(P=0.032);健听组拖延次数显著低于助听器组(P=0.001)和双模式组(P=0.001),极显著低于人工耳蜗组(P<0.001);②不同性别听障儿童语速、停顿、重复次数和拖延次数差异无统计学意义(P>0.05),而助听器组语速显著高于人工耳蜗组(P=0.045),人工耳蜗组停顿次数显著高于助听器组(P=0.028);3.5~5岁听障儿童语速显著低于5.1~6.5岁组(P=0.042);语训0~2.5年听障儿童语速显著低于语训>2.5年者(P=0.002),停顿及重复次数均高于语训>2.5年者(分别P=0.047,P=0.02).结论 听障儿童的语速低于健听儿童,停顿次数、拖延次数高于健听儿童;年龄、辅听装置、语训时长影响学龄前听障儿童的言语流畅性,性别影响不大.