三虫半夏白术天麻汤对痰瘀互阻型头痛患者症状及血清同型半胱氨酸的影响

目的探讨痰瘀互阻型头痛与血清同型半胱氨酸（HCY）之间的相关性并观察三虫半夏白术天麻汤对痰瘀互阻型头痛的疗效及对血清HCY的影响。方法检测176例痰瘀互阻型头痛患者的血清HCY水平,并进行头痛积分及头痛中医证候评分。将HCY〉15μmol/L的患者80例随机分为2组。观察组予三虫半夏白术天麻汤,对照组予盐酸氟桂利嗪胶囊、叶酸片、维生素B6口服治疗。观察治疗2周、4周患者HCY水平、头痛积分及中医证候积分的变化情况。结果 176例痰瘀互阻型头痛患者中115例（65.34%）HCY值高于正常值,痰瘀互阻型头痛与HCY水平具有显著相关性。观察组患者头痛积分及中医证候综合积分疗效明显优于对照组。两组治疗4周后HCY值较治疗前明显降低;治疗2周后观察组HCY降低幅度明显低于对照组;治疗4周后,两组HCY降低幅度无显著差异。结论痰瘀互阻型头痛与血清HCY值具有显著相关性;三虫半夏白术天麻汤可改善痰瘀互阻型头痛患者头痛症状及中医证候,疗效显著,且无不良反应。     

完善我国国境口岸传染病应急防控体系的探讨

目的探讨完善我国国境口岸传染病应急防控体系的对策。方法对美国在预防和控制急性传染病方面的规划、监测体系、提高应急能力等方面进行研究,并对目前我国国境口岸传染病防控措施进行分析、评价。结果美国具有完善的突发公共卫生事件应急体系,能有效控制突发传染病的传播,而我国国境口岸传染病应急防控体系还存在不足。结论结合口岸实际情况,借鉴美国的经验,建立更为合理、有效的国境口岸传染病应急防控体系。     

中外医学对翼状胬肉的认识和治疗简史

翼状胬肉作为最古老的常见眼表疾病,以睑裂区明显增生肥厚的球结膜及其下的纤维组织血管横跨角膜缘呈三角形长入角膜为主要特征,中医学通常称之为“攀睛”或“胬肉攀睛”.随着古今中外对该病认识的深入及治疗的进步,20世纪80年代以来,高增殖及角膜缘干细胞缺乏的病理理论丰富了人们对翼状胬肉的认识,更好地指导了翼状胬肉的手术及药物治疗,取得了较好的疗效.     

HPLC法同时测定茵胆平肝胶囊中栀子苷、龙胆苦苷、芍药苷的含量

目的:建立测定茵胆平肝胶囊中栀子苷、龙胆苦苷、芍药苷含量的方法。方法:采用高效液相色谱法。色谱柱为AgilentTC-C18柱,流动相为乙腈-水(16∶84),检测波长为238nm,进样量为5μL,流速为1.0mL·min-1,柱温为30℃。结果:栀子苷进样量在0.0826～1.652μg范围内、龙胆苦苷进样量在0.1290～2.580μg范围内、芍药苷进样量在0.0446～0.892μg范围内与各自峰面积积分值均呈良好的线性关系;栀子苷、龙胆苦苷、芍药苷的平均回收率分别为99.8%、100.0%、100.1%,RSD分别为1.89%、1.84%、1.82%(n=9)。结论:本法操作简便、结果准确、灵敏度高、重复性好,可用于茵胆平肝胶囊的质量控制。     

抗人DcR3单克隆抗体的制备、鉴定及应用

目的 制备抗DcR3单克隆抗体(mAb),鉴定其生物学特性,并应用于ELISA、Western blot、How cytometry(FCM)检测.方法 以纯化的可溶性DcR3(sDcR3)免疫Balb/c小鼠,采用杂交瘤技术制备抗DcR3 mAb.用Ig亚类EHSA试剂盒鉴定抗DcR3mAb的亚类.用ELISA方法测定抗DcR3 mAb与sDcR3结合的特性,SDS-PAGE鉴定抗DcR3 mAb与SW480细胞上清中DcR3结合的特性,以鉴定mAb的特性.用间接ELISA法检测腹水mAb的效价、亲和常数并进行表位分析.Western blot检测mAb的特异性及应用,并用所获抗DcR3单克隆抗体(mAb)通过流式细胞仪检测肿瘤细胞表面DcR3的表达水平.结果 获得4株可分泌DcR3 mAb的杂交瘤细胞系ZZ-393、ZZ-394、ZZ-151和ZZ-268.其中DcR3 mAb ZZ-268(下文简称为ZZ-268)的Ig亚类为IgGl(k型);腹水效价为1×10-5;亲和常数为1.28×109水平;ZZ-268和ZZ-151可识别与其他2种抗体小同的抗原表位;Westem blot证实,获得的ZZ-268可特异性地识别DcR3;通过流式细胞术可敏感地检测到不同肿瘤细胞表面DcR3的表达水平.结论 获得4株抗DcR3的mAb,其中ZZ-268效价高、特异性强,将此抗体用于膜DcR3与sDcR3的检测分析.     

Alreration of nuclear matrix-intermediate filament system and differential expression of nuclear matrix proteins during human hepatocarcinoma cell differentiation

AIM:To investigate the association between the configurational and compositional changes of nuclear matrix and the differentiation of carcinoma cells. METHODS: Cells cultured with or without 5 x 10"3 mmol/L of hexamethylene bisacetamide (HMBA) on Nickel grids were treated by selective extraction and prepared for whole mount observation under electron microscopy. The samples were examined under transmission electron microscope. Nuclear matrix proteins were selectively extracted and subjected to subcellular proteomics study. The protein expression patterns were analyzed by PDQuest software. Spots of differentially expressed nuclear matrix proteins were excised and subjected to in situ digestion with trypsin. The peptides were analyzed by matrix-assisted laser-desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Data were submitted for database searching using Mascot tool (www.matrixscience.com). RESULTS: The nuclear matrix (NM) and intermediate filament (IF) in SMMC-7721 hepatocarcinoma cells were found relatively sparse and arranged irregularly. The nuclear lamina was non-uniform,and two kinds of filaments were not tightly connected. After induction for differentiation by HMBA,the NM-IF filaments were concentrated and distributed uniformly. The heterogeneous population of filaments,including highly branched utrathin filaments could also be seen in the regular meshwork. The connection between the two kinds of filaments and the relatively thin,condensed and sharply demarcated lamina composed of intermediate-sized filaments was relatively fastened. Meanwhile,21 NM proteins changed remarkably during SMMC-7721 cell differentiation. Four proteins,i.e. mutant Pyst1,hypothetical protein,nucleophosminl,and LBP were downregulated,whereas four other proteins,eIF6,p44 subunit,(3-tubulin,and SIN3B were upregulated with the last one,SR2/ASF found only in the differentiated SMMC-7721 cells. CONCLUSION: The induced differentiation of SMMC-7721 cells by HMBA is accompanied by the configurational changes of nuclear matrix-intermediate filament (NM-IF) system and the compositional changes of nuclear matrix protein expression. These changes may be important morphological or functional indications of the cancer cell reversion.     

MALDI-TOF-MS结合引物延伸反应检测K-ras基因型方法的建立

目的:建立MALDI-TOF-MS结合引物延伸的方法检测K-ras基因12位密码子7种基因型的研究模型并探讨其应用价值.方法:以自行构建的K-ras质粒DNA为模板,经目的片断扩增,去磷酸化,再用两条未经修饰的普通引物,在dATP、ddTTP、ddCTP和ddGTP混和物存在的体系中进行引物延伸反应,最后用MALDI-TOF-MS检测引物延伸产物质荷比(m/z),得到质谱图,根据质谱图中各个峰的峰值进行基因分型研究结果:用MALDI-TOF-MS结合引物延伸的方法,可以准确区分7种不同基因型.该方法可同时对384份样品进行检测结论:MALDI-TOF-MS结合引物延伸的方法准确度高,反应体系稳定并具有快速、高通量、自动化的特点,可能成为肿瘤早期诊断的检测方法.     

厦门市泌尿生殖道支原体检测与药敏分析

目的调查厦门地区Uu．Mh的感染和药敏情况，指导临床合理用药。方法应用珠海黑马公司提供的支原体培养鉴定试剂盒检测1453例患者。结果1453例患者检出支原体723例（49．76％），其中uu感染539例（37．10％），Mh48例（8．8％）；单Mh感染26例（1．79％）；混合感染158例（10．87％）。女性感染677例（50．83％），男性感染46例（38．02％）。感染类型和性别之间有显著性差异（P〈0．05）。药敏结果：首选为克拉霉素、强力霉素、交沙霉素和美满霉素，其次为司帕沙星和罗红霉素。结论支原体uu、Mh引发泌尿生殖道感染有逐增趋势，加强监测对防治支原体感染是很有必要的。     

羊膜移植治疗角膜溃疡的初步报告

目的 :评价羊膜移植在角膜溃疡治疗中的临床疗效。方法 :采用羊膜移植治疗细菌性角膜溃疡 4只眼 ,病毒性角膜溃疡 4只眼 ,角膜溃疡穿孔 4只眼。术后随访 3～ 14个月。结果 :术后 3～ 10天炎症控制。 4例角膜穿孔瘢痕愈合 ,5例角膜溃疡留下不同程度角膜瘢翳。结论 :羊膜移植治疗角膜溃疡 ,是一种有价值的治疗方法     

论医疗纠纷的调解

医疗纠纷呈逐年上升趋势,并演化成为现代社会一种多发性的纠纷类型。由于调解在纠纷的解决上表现出来的简便、经济、快捷、灵活等优点,调解逐渐为人们所重视,鼓励和发展医疗纠纷的调解不失为一条快速、有效的解决医疗纠纷的途径。