The treatment of hypertrophy of the prostate of rats with 4-nitro-3-trifluoromethylisobutyranilide (Sch 13521).

A study was carried out in 146 common rats to assess the effects of 4'-nitro-3'-trifluoromethylisobutyranilide (Sch 13521) on the hypertrophied prostate gland. The hypertrophy was induced in castrated, sexually immature males by s.c. implantation of a pill of testosterone-propionate. After 20 days of administration of Sch 13521 in oral doses of 5, 10 and 25 mg/kg, the dimensions and weight of the hypertrophied prostate gland were reduced by 1.5 to 2 times. After 2 months of administration of the preparation in doses of 10 and 25 mg/kg a reduction of the hypertrophied prostate gland to the dimensions of the intact control was observed. Morphological studies confirmed the efficacy of the therapeutic action of Sch 13521.     

Autonomic reactions in rabbits exposed to a constant magnetic field]

Chronic experiments on rabbits have shown that their exposure to a constant magnetic field of 4500 oersted for 3 hours causes a transient hypotension, a decrease in the respiratory rate and a trend for bradycardia. Immediately after the exposure the pressor reaction and bradycardia level decrease and by the end of the first day they increase. At this time the respiratory rate increases in response to the administration of epinephrine, acethylcholine or stimulation of the midbrain reticular formation. The exposure does not decrease the compensatory possibilities of the cardiovascular and respiratory systems. This follows from their reactions to accelerations of 6 and 10 g.     

Regulation of miRNA expression during neural cell specification

MicroRNA (miRNA) are a newly recognized class of small, noncoding RNA molecules that participate in the developmental control of gene expression. We have studied the regulation of a set of highly expressed neural miRNA during mouse brain development. Temporal control is a characteristic of miRNA regulation in C. elegans and Drosophila, and is also prominent in the embryonic brain. We observed significant differences in the onset and magnitude of induction for individual miRNAs. Comparing expression in cultures of embryonic neurons and astrocytes we found marked lineage specificity for each of the miRNA in our study. Two of the most highly expressed miRNA in adult brain were preferentially expressed in neurons (mir-124, mir-128). In contrast, mir-23, a miRNA previously implicated in neural specification, was restricted to astrocytes. mir-26 and mir-29 were more strongly expressed in astrocytes than neurons, others were more evenly distributed (mir-9, mir-125). Lineage specificity was further explored using reporter constructs for two miRNA of particular interest (mir-125 and mir-128). miRNA-mediated suppression of both reporters was observed after transfection of the reporters into neurons but not astrocytes. miRNA were strongly induced during neural differentiation of embryonic stem cells, suggesting the validity of the stem cell model for studying miRNA regulation in neural development.     

Epithelio-myoepithelial carcinoma of the salivary glands

Epithelio-myoepithelial carcinoma was singled out as a separate nosological unit in the second revision of the International Histological Classification of Tumors of the Salivary glands (WHO, 1991). For the first time in domestic literature the article describes morphological evidence obtained at investigation of epithelial-myoepithelial carcinoma of the parotid gland using modern technologies.