全文获取类型
收费全文 | 1167篇 |
免费 | 52篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 78篇 |
妇产科学 | 12篇 |
基础医学 | 96篇 |
口腔科学 | 26篇 |
临床医学 | 103篇 |
内科学 | 272篇 |
皮肤病学 | 21篇 |
神经病学 | 66篇 |
特种医学 | 256篇 |
外科学 | 122篇 |
综合类 | 11篇 |
一般理论 | 1篇 |
预防医学 | 39篇 |
眼科学 | 45篇 |
药学 | 32篇 |
中国医学 | 1篇 |
肿瘤学 | 42篇 |
出版年
2022年 | 5篇 |
2021年 | 3篇 |
2018年 | 14篇 |
2017年 | 11篇 |
2016年 | 8篇 |
2015年 | 15篇 |
2014年 | 20篇 |
2013年 | 28篇 |
2012年 | 28篇 |
2011年 | 24篇 |
2010年 | 42篇 |
2009年 | 44篇 |
2008年 | 31篇 |
2007年 | 28篇 |
2006年 | 35篇 |
2005年 | 21篇 |
2004年 | 19篇 |
2003年 | 27篇 |
2002年 | 15篇 |
2001年 | 14篇 |
2000年 | 11篇 |
1999年 | 12篇 |
1998年 | 50篇 |
1997年 | 68篇 |
1996年 | 50篇 |
1995年 | 43篇 |
1994年 | 53篇 |
1993年 | 53篇 |
1992年 | 20篇 |
1991年 | 22篇 |
1990年 | 20篇 |
1989年 | 42篇 |
1988年 | 45篇 |
1987年 | 30篇 |
1986年 | 33篇 |
1985年 | 33篇 |
1984年 | 11篇 |
1983年 | 25篇 |
1982年 | 17篇 |
1981年 | 20篇 |
1980年 | 21篇 |
1979年 | 11篇 |
1978年 | 14篇 |
1977年 | 14篇 |
1976年 | 14篇 |
1975年 | 21篇 |
1974年 | 4篇 |
1972年 | 3篇 |
1969年 | 3篇 |
1967年 | 6篇 |
排序方式: 共有1224条查询结果,搜索用时 18 毫秒
81.
Synthesis of glycosaminoglycans by cultures of normal human corneal endothelial and stromal cells 总被引:3,自引:0,他引:3
Monolayer cultures of normal human corneal endothelial and stromal cells were incubated with [35S]sulfate and [3H]glucosamine for 4 hr. The labeled glycosaminoglycans resulted from this incubation were isolated from the cell layer and the growth medium and further characterized. Both endothelial and stromal cell cultures synthesized a variety of sulfated glycosaminoglycans, with chondroitin 6-sulfate as the major product. Chondroitin 4-sulfate, dermatan sulfate, and heparan sulfate were present in smaller amounts. Keratan sulfate was produced only in minimal amounts. Both cell types also synthesized hyaluronic acid. The hyaluronic acid production in stromal cell strains derived from donors of different ages was similar. The demonstration that the endothelial cell strain derived from a 1-day-old baby contained more hyaluronic acid than cultures from older donors suggests a possible age-related phenomenon as seen in developing tissues. 相似文献
82.
83.
84.
Madhotra D Fenton JE Makura ZG Charters P Roland NJ 《Irish journal of medical science》2004,173(4):197-199
Background The timing of aggressive airway intervention in adult epiglottitis is controversial.
Aims To correlate Friedman’s staging of epiglottitis on admission with the airway interventions undertaken.
Methods A retrospective study of 23 adult patients, mean age 51 years (range 29–81 years), who had been admitted with acute supraglottitis
between March 1988 and December 2000 was undertaken.
Results Three patients (13%) had airway interventions; two with tracheostomy and one with tracheal intubation. All were Friedman
stage III and had rapid symptom progression during the 24 hours prior to admission. Three other stage III patients with symptom
progression longer than 24 hours and all the remaining patients (stage II or less) were managed with observation and intravenous
therapy.
Conclusions Friedman originally advocated airway intervention in any patient stage II or worse, but this intubation threshold should
probably be lowered to those patients with rapid-onset stage III (moderate respiratory distress, stridor, respiratory rate
>30 per minute, pCO2 >45mmHg) disease. 相似文献
85.
Silbert S Boyken L Hollis RJ Pfaller MA 《Diagnostic microbiology and infectious disease》2003,47(4):619-621
Although pulsed-field gel electrophoresis is considered the "gold standard" technique for molecular typing, typeability may not be excellent for some bacterial species because of DNA degradation. Previous reports suggest that the addition of thiourea in the gel buffer can improved the typeability for some species. In the present study, 66 Gram-negative strains (seven species) known to be affected by DNA degradation and four control strains were evaluated by PFGE with and without the addition of 50 microg/M of thiourea to the buffer used in the electrophoresis. Macrorestriction patterns were obtained for all K. pneumoniae, S. marcescens, P. aeruginosa, and Salmonella spp., for 95.4% of E. coli, and for 50% of E. cloacae strains from the gels performed in the buffer with throurea. However, typeability was not improved for Acinetobacter spp. The range of non-typeable species for which thiourea can limit the problem of DNA degradation is considerably wider than described in previous publications. 相似文献
86.
AM Pierides HA Ellis H Dellagrammatikas JE Scott AW Norman 《Archives of disease in childhood》1977,52(6):464-472
Three children with azotaemic renal osteodystrophy were treated with 1,25-dihydroxycholecalciferol (1,25(OH)2D3). All showed clinical, biochemical, and radiological improvement within 6 months of starting treatment. There were no complications. The dose of 1,25(OH)2D3 required was 0-5 microgram per day for 2 children aged 22 and 30 months, and 2 microgram per day for a 15-year-old boy. 2 of the patients were receiving phenobarbitone and phenytoin and in one of them prior treatment with dihydrotachysterol 0-5 mg daily and 6 microgram 1alpha-hydroxycholecalciferol (1alphaOHD3) daily had failed to induce improvement. In one patient, in whom serial iliac bone samples were available, 2 microgram 1,25(OH)2D3 resulted in histological improvement in previously severe osteomalacia. 1,25(OH)2D3 appears to be an effective and safe drug in the treatment of uraemic osteodystrophy. 相似文献
87.
88.
89.
90.
A Nørremølle E Budtz-Jørgensen K Fenger JE Nielsen SA Sørensen and L Hasholt 《Clinical genetics》2009,75(3):244-250
Huntington disease (HD) is caused by an expanded CAG repeat sequence in the HD gene. Although the age at onset is correlated to the CAG repeat length, this correlation only explains approximately half of the variation in onset age. Less variation between siblings indicates that the variation is, in part, explained by genetic modifiers. We analyzed polymorphic loci within or close to the HD gene on the HD chromosome in Danish HD patients. We found one specific haplotype segregating with later age at onset, compared with patients with similar CAG repeat length and another haplotype. The nine Danish families in the study carrying this haplotype most likely have a common founder. Several of the polymorphic loci displayed alleles that may be specific to the late-onset haplotype, implicating that from this study we cannot determine which of the loci tested (or other polymorphic loci in this chromosomal area) do in fact contain genetic modifiers of age at onset. 相似文献