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The multi-scan resting state fMRI (rs-fMRI) dataset was recently released; thus the test-retest (TRT) reliability of rs-fMRI measures can be assessed. However, because this dataset was acquired only from a single group under a single condition, we cannot directly evaluate whether the rs-fMRI measures can generate reproducible between-condition or between-group results. Because the modulation of resting state activity has gained increasing attention, it is important to know whether one rs-fMRI metric can reliably detect the alteration of the resting activity. Here, we shared a public Eyes-Open (EO)/Eyes-Closed (EC) dataset for evaluating the split-half reproducibility of the rs-fMRI measures in detecting changes of the resting state activity between EO and EC. As examples, we assessed the split-half reproducibility of three widely applied rs-fMRI metrics: amplitude of low frequency fluctuation, regional homogeneity, and seed-based correlation analysis. Our results demonstrated that reproducible patterns of EO-EC differences can be detected by all three measures, suggesting the feasibility of the EO/EC dataset for performing reproducibility assessment for other rs-fMRI measures. 相似文献
54.
目的:建立人原发性直肠恶性淋巴瘤裸小鼠原位移植模型,探讨其生物学特性.方法:采用人直肠原发性恶性淋巴瘤术中的新鲜瘤组织块植入裸鼠的直肠黏膜层内,观察原位移植的成瘤率,移植瘤的侵袭和转移率.进行形态学(光镜、电镜、免疫组织化学),染色体核型,流式细胞分析.结果:依据WHO新的分类标准,建成1株人直肠原发性(非霍奇金B细胞性)恶性淋巴瘤裸鼠原位移植模型HRBL-0305.移植瘤组织病理学为(非霍奇金B细胞性)高度恶性淋巴瘤;免疫组织化学示CD19,CD20,CD22,CD45阳性,CD3,CD7阴性.染色体众数56-69条,流式细胞DI值为1.57-1.61,均为异倍体.HRBL-0305已传至31代;共移植裸鼠187只.其肿瘤移植生长率和液氮冻存复苏成活率均为100.0%,肝转移率为45.4%,淋巴结和腹腔种植转移率均为38.0%,移植瘤在裸鼠的直肠内自主侵袭性生长,发生血液、淋巴转移和腹腔内种植性转移,移植瘤组织病理学,超微结构的观察,流式细胞DNA含量测定及染色体核型的分析,表明与人源直肠恶性淋巴瘤细胞相一致.结论:HRBL-0305是首次成功建立的人直肠原发性恶性淋巴瘤裸鼠原位移植模型,完整地重现了人直肠原发性恶性淋巴瘤的自然临床病理过程,且转移模式与临床患者相似,为研究直肠恶性淋巴瘤的生物学特性和治疗提供了理想动物模型平台. 相似文献
55.
Xiang Yu Song Xiaona Qiao Jing Zang Yimei Li Yanpeng Liu Yong Liu Chunsheng 《Journal of natural medicines》2015,69(3):278-286
Journal of Natural Medicines - An efficient simplified method was developed to determine multiple classes of phytohormones simultaneously in the medicinal plant Glycyrrhiza uralensis.... 相似文献
56.
Background:To explore the association of chronic rhinosinusitis (CRS) with bronchial asthma (BA) as well as its severity.Methods:A comprehensive database search will be performed from PubMed, Embase, Cochrane Library, and Web of science for related literatures. Heterogeneity test will be used to assess each outcome indicator. If heterogeneity statistics I2 ≥ 50%, the random effects model will be applied; if I2 < 50%, the fixed effects model will be performed. Sensitivity analysis will be performed in all models. STATA 15.0 software (Stata Corporation, College Station, TX) will be used for statistical analysis. Risk ratio (RR) will be used as the effect size for enumeration data. P < .05 is considered statistically significant.Conclusion:This study will evaluate the association of CRS with the prevalence of BA as well as its severity.OSF registration number:10.17605/OSF.IO/GCTM9. 相似文献
57.
Background:Cancer is a life-threatening condition and also one of the biggest challenges facing human health and the medical community. This meta-analysis was to investigate the effects of music intervention on physiological and psychological responses of patients with cancer.Methods and analysis:The following electronic databases will be searched from inception to December 2020: PubMed, EMBASE, Web of Science, Chinese Biomedical Literature Database, Chinese National Knowledge Infrastructure Database, Chinese Science and the Wanfang Database. We only included music intervention vs placebo in cancer patients and pooled results were summarized by STATA 12.0 software. Two investigators independently selected the studies according to the inclusion and exclusion criteria, extracted the data, and assessed the quality of the selected studies. The existence of statistical heterogeneity would be evaluated by Chi2 test and its extension by the I2 test (I2 > 50% indicates high heterogeneity among studies). Publication bias was ruled out by funnel plot and statistically assessed by Begg test (P > .05 as no publication bias).Results:The study results will be published in relevant peer-reviewed journals and key findings will be presented at international scientific meetings.Conclusion:Our study aims to systematically assess the effects of music intervention in cancer patients, which will be provide clinical guidance for cancer patients. 相似文献
58.
X. F. Zhang B. Wang J. Yu X.
N. Wu Y. H. Zang H. C. Gao P. C. Su S. Q. Hao 《RSC advances》2018,8(3):1159
In this study, porous carbon (3DHPC) with a 3D honeycomb-like structure was synthesized from waste biomass corncob via hydrothermal carbonization coupled with KOH activation and investigated as a capacitive deionization (CDI) electrode material. The obtained 3DHPC possesses a hierarchal macroporous and mesoporous structure, and a large accessible specific surface area (952 m2 g−1). Electrochemical tests showed that the 3DHPC electrode exhibited a specific capacitance of 452 F g−1 and good electric conductivity. Moreover, the feasibility of electrosorptive removal of chromium(vi) from an aqueous solution using the 3DHPC electrode was demonstrated. When 1.0 V was applied to a solution containing 30 mg L−1 chromium(vi), the 3DHPC electrode exhibited a higher removal efficiency of 91.58% compared with that in the open circuit condition. This enhanced adsorption results from the improved affinity between chromium(vi) and the electrode under electrochemical assistance involving a non-faradic process. Consequently, the 3DHPC electrode with typical double-layer capacitor behavior is demonstrated to be a favorable electrode material for capacitive deionization.A porous carbon electrode with a 3D honeycomb-like structure demonstrates a high removal efficiency for the removal of chromium(vi) from water. 相似文献
59.
Dong Wang Lichun An Jiangke Tian Tengfei Yu Xia Xie Yuejuan Gao Yanfen Zang Yanyan Tao Yanqing Liu Ying Jin 《The American journal of emergency medicine》2018,36(9):1627-1630
Background
Real-time ultrasound elastography (RTE) is used to examine liver fibrosis and benign and malignant lesions, but its use for the diagnosis of liver trauma has not been examined. The purpose of this study was to examine the use of RTE for the evaluation of differential penetrating liver trauma in a rabbit model.Material and methods
Eighty New Zealand rabbits were divided into 2 groups. In one group, a single incision (type “-” lesion) was made, and in the other group a hash mark incision (type “#” lesion) was made (about 0.5 cm in depth; 1.0–2.0 cm in length). RTE was performed at 10, 30, and 60 min after injury.Results
There were no differences in mean RTE scores between the 2 types of lesions at 10 and 30 min. However, the mean values for the 2 types of lesions increased from 10 min to 60 min (type ‘-’ lesion: 0.88 ± 0.32 to 2.06 ± 0.88; type ‘#’ lesion: 0.89 ± 0.34 to 2.63 ± 1.16). At 60 min, the mean elasticity score in the type ‘#’ lesion group was significantly higher than in the type ‘-’ lesion group (P < .001). Strain ratios were not different between the groups at each time point, but in each group the values decreased from the 10 min time point to the 60 min time point (P-value for the trends, <.001).Conclusions
RTE may be able to distinguish mild or severe penetrating liver trauma at 60 min or more after injury. 相似文献60.
Xing‐Zhu Lu Xue‐Yuan Bi Xi He Ming Zhao Man Xu Xiao‐Jiang Yu Zheng‐Hang Zhao Wei‐Jin Zang 《British journal of pharmacology》2015,172(23):5619-5633
Background and Purpose
The activation of M 3 cholinoceptors (M 3 receptors) by choline reduces cardiovascular risk, but it is unclear whether these receptors can regulate ischaemia/reperfusion (I/R)‐induced vascular injury. Thus, the primary goal of the present study was to explore the effects of choline on the function of mesenteric arteries following I/R, with a major focus on Ca2+/calmodulin‐dependent protein kinase II (CaMKII) regulation.Experimental Approach
Rats were given choline (10 mg·kg−1, i.v.) and then the superior mesenteric artery was occluded for 60 min (ischaemia), followed by 90 min of reperfusion. The M 3 receptor antagonist, 4‐diphenylacetoxy‐N‐methylpiperidine methiodide (4‐DAMP), was injected (0.12 μg·kg−1, i.v.) 5 min prior to choline treatment. Vascular function was examined in rings of mesenteric arteries isolated after the reperfusion procedure. Vascular superoxide anion production, CaMKII and the levels of Ca2+‐cycling proteins were also assessed.Key Results
Choline treatment attenuated I/R‐induced vascular dysfunction, blocked elevations in the levels of reactive oxygen species (ROS) and decreased the up‐regulated expression of oxidised CaMKII and phosphorylated CaMKII. In addition, choline reversed the abnormal expression of Ca2+‐cycling proteins, including Na+/Ca2+ exchanger, inositol 1,4,5‐trisphosphate receptor, sarcoplasmic reticulum Ca2+‐ATPase and phospholamban. All of these cholinergic effects of choline were abolished by 4‐DAMP.Conclusions and Implications
Our data suggest that inhibition of the ROS‐mediated CaMKII pathway and modulation of Ca2+‐cycling proteins may be novel mechanisms underlying choline‐induced vascular protection. These results represent a significant addition to the understanding of the pharmacological roles of M 3 receptors in the vasculature, providing a new therapeutic strategy for I/R‐induced vascular injury.Linked Articles
This article is part of a themed section on Chinese Innovation in Cardiovascular Drug Discovery. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2015.172.issue-23Abbreviations
- 4‐DAMP
- 4‐diphenylacetoxy‐N‐methylpiperidine methiodide
- CaMKII
- Ca2+/calmodulin‐dependent protein kinase II
- DHE
- dihydroethidium
- I/R
- ischaemia/reperfusion
- IP3R
- inositol 1,4,5‐trisphosphate receptor
- NAC
- N‐acetyl‐L‐cysteine
- NCX
- Na+/Ca2+ exchanger
- PLB
- phospholamban
- ROS
- reactive oxygen species
- SERCA
- sarcoplasmic reticulum Ca2+‐ATPase
- SNP
- sodium nitroprusside
TARGETS |
---|
GPCRs a |
M3 receptors |
Enzymes b |
SERCA 2, sarcoplasmic reticulum Ca2+‐ATPase |
Ion channels c |
NCX1, Na+/Ca2+ exchanger |
Ligand‐gated ion channels d |
IP3R, inositol 1,4,5‐trisphosphate receptor |
LIGANDS |
---|
4‐DAMP, 4‐diphenylacetoxy‐N‐methylpiperidine methiodide |
5‐HT |
ACh |
Caffeine |
Choline |
Darifenacin |
KN‐93 |
L‐NAME, NG‐nitro‐L‐arginine methyl ester |
Phenylephrine |