全文获取类型
收费全文 | 8344篇 |
免费 | 371篇 |
国内免费 | 79篇 |
专业分类
耳鼻咽喉 | 72篇 |
儿科学 | 136篇 |
妇产科学 | 166篇 |
基础医学 | 882篇 |
口腔科学 | 147篇 |
临床医学 | 524篇 |
内科学 | 2206篇 |
皮肤病学 | 181篇 |
神经病学 | 553篇 |
特种医学 | 390篇 |
外科学 | 1583篇 |
综合类 | 19篇 |
一般理论 | 1篇 |
预防医学 | 234篇 |
眼科学 | 228篇 |
药学 | 391篇 |
中国医学 | 15篇 |
肿瘤学 | 1066篇 |
出版年
2024年 | 9篇 |
2023年 | 65篇 |
2022年 | 134篇 |
2021年 | 216篇 |
2020年 | 124篇 |
2019年 | 154篇 |
2018年 | 230篇 |
2017年 | 158篇 |
2016年 | 182篇 |
2015年 | 215篇 |
2014年 | 258篇 |
2013年 | 339篇 |
2012年 | 572篇 |
2011年 | 649篇 |
2010年 | 361篇 |
2009年 | 320篇 |
2008年 | 593篇 |
2007年 | 601篇 |
2006年 | 542篇 |
2005年 | 531篇 |
2004年 | 465篇 |
2003年 | 474篇 |
2002年 | 421篇 |
2001年 | 88篇 |
2000年 | 82篇 |
1999年 | 104篇 |
1998年 | 114篇 |
1997年 | 68篇 |
1996年 | 75篇 |
1995年 | 48篇 |
1994年 | 66篇 |
1993年 | 45篇 |
1992年 | 50篇 |
1991年 | 38篇 |
1990年 | 35篇 |
1989年 | 39篇 |
1988年 | 35篇 |
1987年 | 33篇 |
1986年 | 20篇 |
1985年 | 22篇 |
1984年 | 24篇 |
1983年 | 20篇 |
1982年 | 23篇 |
1981年 | 18篇 |
1980年 | 12篇 |
1979年 | 8篇 |
1978年 | 26篇 |
1977年 | 15篇 |
1975年 | 8篇 |
1968年 | 9篇 |
排序方式: 共有8794条查询结果,搜索用时 0 毫秒
141.
142.
Miwa Haruta Yusuke Tomita Yuya Imamura Keiko Matsumura Tokunori Ikeda Koutaro Takamatsu Yasuharu Nishimura Satoru Senju 《Human immunology》2013
Anticancer vaccination therapies with monocyte-derived dendritic cells (DC) are widely conducted. A large number of primary monocytes (approximately 108 cells) are needed to generate the number of DC required to achieve an effect upon vaccination, and monocytes are usually purified from peripheral blood mononuclear cells obtained by apheresis procedure, which is somehow invasive for cancer patients. As a means to facilitate the generation of DC for therapeutic use, we herein report a method to amplify human monocytes. We found that lentivirus-mediated transduction of cMYC along with BMI1 induced proliferation of CD14+ monocytes derived from 9 out of 12 blood donors, and we named the monocyte-derived proliferating cells CD14-ML. Their proliferation continued for 3–5 weeks in the presence of M-CSF and GM-CSF, resulting in 20–1000-fold amplification. Importantly, the expanded CD14-ML differentiated into fully functional DC (CD14-ML-DC) upon the addition of IL-4 to the culture. We successfully stimulated autologous CD8+ T cells with CD14-ML-DC pulsed with cytomegalovirus peptide or MART-1 peptide to generate antigen-specific CTL lines. This is the first report describing the method for in vitro expansion of human peripheral blood monocytes. 相似文献
143.
Sandra Rayego‐Mateos Jose Luis Morgado‐Pascual Ana Belen Sanz Adrian Mario Ramos Satoru Eguchi Daniel Batlle Janos Pato Gyorgy Keri Jesus Egido Alberto Ortiz Marta Ruiz‐Ortega 《The Journal of pathology》2013,231(4):480-494
TWEAK, a member of the TNF superfamily, binds to the Fn14 receptor, eliciting biological responses. EGFR signalling is involved in experimental renal injury. Our aim was to investigate the relationship between TWEAK and EGFR in the kidney. Systemic TWEAK administration into C57BL/6 mice increased renal EGFR phosphorylation, mainly in tubular epithelial cells. In vitro, in these cells TWEAK phosphorylated EGFR via Fn14 binding, ADAM17 activation and subsequent release of the EGFR ligands HB‐EGF and TGFα. In vivo the EGFR kinase inhibitor Erlotinib inhibited TWEAK‐induced renal EGFR activation and downstream signalling, including ERK activation, up‐regulation of proinflammatory factors and inflammatory cell infiltration. Moreover, the ADAM17 inhibitor WTACE‐2 also prevented those TWEAK‐induced renal effects. In vitro TWEAK induction of proinflammatory factors was prevented by EGFR, ERK or ADAM17 inhibition. In contrast, EGFR transactivation did not modify TWEAK‐mediated NF‐κB activation. Our data suggest that TWEAK transactivates EGFR in the kidney, leading to modulation of downstream effects, including ERK activation and inflammation, and suggest that inhibition of EGFR signalling could be a novel therapeutic tool for renal inflammation. Copyright © 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. 相似文献
144.
Kanako Iwasaki Tomoyuki Fujiyama Shinya Nakata Minjeong Park Chika Miyoshi Noriko Hotta-Hirashima Aya Ikkyu Miyo Kakizaki Fumihiro Sugiyama Seiya Mizuno Manabu Abe Kenji Sakimura Satoru Takahashi Hiromasa Funato Masashi Yanagisawa 《The Journal of neuroscience》2021,41(12):2733
Sleep is regulated in a homeostatic manner. Sleep deprivation increases sleep need, which is compensated mainly by increased EEG δ power during non-rapid eye movement sleep (NREMS) and, to a lesser extent, by increased sleep amount. Although genetic factors determine the constitutive level of sleep need and sleep amount in mice and humans, the molecular entity behind sleep need remains unknown. Recently, we found that a gain-of-function Sleepy (Slp) mutation in the salt-inducible kinase 3 (Sik3) gene, which produces the mutant SIK3(SLP) protein, leads to an increase in NREMS EEG δ power and sleep amount. Since Sik3Slp mice express SIK3(SLP) in various types of cells in the brain as well as multiple peripheral tissues from the embryonic stage, the cell type and developmental stage responsible for the sleep phenotype in Sik3Slp mice remain to be elucidated. Here, we generated two mouse lines, synapsin1CreERT2 and Sik3ex13flox mice, which enable inducible Cre-mediated, conditional expression of SIK3(SLP) in neurons on tamoxifen administration. Administration of tamoxifen to synapsin1CreERT2 mice during late infancy resulted in higher recombination efficiency than administration during adolescence. SIK3(SLP) expression after late infancy increased NREMS and NREMS δ power in male synapsin1CreERT2; Sik3ex13flox/+ mice. The expression of SIK3(SLP) after adolescence led to a higher NREMS δ power without a significant change in NREMS amounts. Thus, neuron-specific expression of SIK3(SLP) after late infancy is sufficient to increase sleep.SIGNIFICANCE STATEMENT The propensity to accumulate sleep need during wakefulness and to dissipate it during sleep underlies the homeostatic regulation of sleep. However, little is known about the developmental stage and cell types involved in determining the homeostatic regulation of sleep. Here, we show that Sik3Slp allele induction in mature neurons in late infancy is sufficient to increase non-rapid eye movement sleep amount and non-rapid eye movement sleep δ power. SIK3 signaling in neurons constitutes an intracellular mechanism to increase sleep. 相似文献
145.
Hirokazu Oguni Susumu Ito Aiko Nishikawa Yui Otani Satoru Nagata 《Brain & development》2021,43(7):775-782
AimTo elucidate the morphological characteristics of spike-wave complexes (SWCs) causing myoclonic seizures (MS) in childhood-onset idiopathic myoclonic epilepsies.Subjects and MethodsThe subjects were 8 patients, including 4 with epilepsy with myoclonic-atonic seizures (EMAS), 3 with myoclonic epilepsy in infancy (MEI) and 1 with idiopathic unclassifiable myoclonic epilepsy. Morphometric parameters of the SWCs were compared between those with MS [SWC-MS (+)] and those without MS [SWC-MS (-)], and a correlation coefficient analysis was performed between the SWC parameters and the duration of myoclonic electromyogram (EMG) potentials.ResultsA total of 155 SWC-MS (+) (range: 7 ~ 34) and 80 SWC-MS (-) (10 each as a control) were analyzed. Comparison of the parameters of the SWCs between SWC-MS (+) and SWC-MS (-) demonstrated that the depth and the width of the positive-sharp-components (PSC) in the SWC-MS (+) were significantly deeper in amplitude and longer in duration than those in the SWC-MS (-), respectively, in all 8 patients (P < 0.05), whereas the number of the polyphasic-multiple-spike-components (PMSC) and the height of negative-spike-components (NSC) were not significantly different in most of the patients, respectively. The depth and the width of PSC were also significantly correlated with the duration of myoclonic EMG potentials in all patients except one [depth of PSC (n = 7): r = 0.623 ~ 0.888; width of PSC (n = 8): r = 0.676 ~ 0.948, P < 0.05].ConclusionsThis study revealed that the depth and width of PSC of the SWC are positively correlated with the MS intensity in childhood-onset idiopathic myoclonic epilepsies and are an important neurophysiological marker to generate MS. 相似文献
146.
Yoshitane Tsukamoto Yasuo Nakata Hiroyuki Futani Satoru Fukunaga Kazuyoshi Kajimoto Seiichi Hirota 《Pathology, research and practice》2013
Clear cell sarcoma is a unique tumor which has EWSR1-ATF1 or EWSR1-CREB1 fusion. Several patterns of EWSR1-ATF1 fusion are observed in clear cell sarcoma. Since type 5–7 fusions were reported recently, they are classified as type 1–7. We examined EWSR1-ATF1 and EWSR1-CREB1 fusions in a single case of clear cell sarcoma with lung metastasis in a 36-year-old Japanese man. As a result, we found only type 1 EWSR1-ATF1 fusion in the primary site, but 4 types of EWS-ATF1 fusion (type 1, 2, 5, 6) were detected in the metastatic site. These 4 types of fusion were completely identical to the recent report, but the case had the same fusion patterns in both primary and metastatic sites. In our case, increased splicing activity in the EWSR1-ATF1 fusion might be acquired at the metastatic site. There is another possibility that metastasis might develop through the increased splicing activity in the fusion. 相似文献
147.
Shin Urai Naoko Hashimoto Michinori Takabe Yuya Kashitani Yuka Satake Yuki Nishimoto Motoharu Kawashima Soichiro Henmi Mitsuo Kuroda Yu Yamane Tokito Koga Satoru Kajita Kazuki Doi Tetsuya Oue Hirohisa Murakami Nobuhiko Mukohara Takeshi Ohara 《Journal of artificial organs》2021,24(2):225-233
Journal of Artificial Organs - Perioperative hyperglycemia, hypoglycemia, and high glycemic variability are independent risk factors for mortality in critically ill patients. After cardiac surgery,... 相似文献
148.
Tanaka Takashi Nakada Takayuki Ito Tetsufumi Kominami Rieko Sonomura Takahiro Kagaya Miyuki Kawai Katsushi Honma Satoru 《Anatomical science international / Japanese Association of Anatomists》2021,96(1):112-118
Anatomical Science International - Hepatic biliary injury is one of the most common complications in cholecystectomy and is frequently accompanied by arterial injuries. Because there are several... 相似文献
149.