全文获取类型
收费全文 | 19859篇 |
免费 | 2022篇 |
国内免费 | 31篇 |
专业分类
耳鼻咽喉 | 271篇 |
儿科学 | 736篇 |
妇产科学 | 542篇 |
基础医学 | 2848篇 |
口腔科学 | 634篇 |
临床医学 | 2406篇 |
内科学 | 3674篇 |
皮肤病学 | 372篇 |
神经病学 | 1704篇 |
特种医学 | 880篇 |
外国民族医学 | 1篇 |
外科学 | 2352篇 |
综合类 | 535篇 |
一般理论 | 9篇 |
预防医学 | 2052篇 |
眼科学 | 284篇 |
药学 | 1550篇 |
中国医学 | 24篇 |
肿瘤学 | 1038篇 |
出版年
2021年 | 288篇 |
2020年 | 178篇 |
2019年 | 294篇 |
2018年 | 351篇 |
2017年 | 278篇 |
2016年 | 298篇 |
2015年 | 334篇 |
2014年 | 447篇 |
2013年 | 653篇 |
2012年 | 809篇 |
2011年 | 839篇 |
2010年 | 558篇 |
2009年 | 490篇 |
2008年 | 856篇 |
2007年 | 837篇 |
2006年 | 868篇 |
2005年 | 738篇 |
2004年 | 739篇 |
2003年 | 665篇 |
2002年 | 671篇 |
2001年 | 630篇 |
2000年 | 660篇 |
1999年 | 547篇 |
1998年 | 308篇 |
1997年 | 256篇 |
1996年 | 272篇 |
1995年 | 249篇 |
1994年 | 222篇 |
1993年 | 251篇 |
1992年 | 458篇 |
1991年 | 437篇 |
1990年 | 439篇 |
1989年 | 454篇 |
1988年 | 451篇 |
1987年 | 361篇 |
1986年 | 378篇 |
1985年 | 400篇 |
1984年 | 271篇 |
1983年 | 224篇 |
1982年 | 179篇 |
1981年 | 167篇 |
1979年 | 215篇 |
1978年 | 192篇 |
1977年 | 207篇 |
1976年 | 178篇 |
1975年 | 167篇 |
1974年 | 172篇 |
1973年 | 182篇 |
1972年 | 169篇 |
1971年 | 164篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
131.
Fraser KP Gays F Robinson JH van Beneden K Leclercq G Vance RE Raulet DH Brooks CG 《European journal of immunology》2002,32(3):868-878
NK cells developing in vitro from fetal progenitors in the presence of IL-2 are phenotypically and functionally indistinguishable from mature adult NK cells, with the exception that they generally lack surface expression of any of the Ly49 molecules that have previously been examined. Using two recently developed anti-Ly49 mAb, we show here that most of these NK cells in fact express high levels of at least one previously uncharacterized member of the Ly49 family, most likely Ly49E. Detailed kinetic and clonal analysis revealed that these Ly49 molecules were acquired in a progressive and stochastic manner independently of CD94 and NKG2. CD94 and NKG2 were both expressed early in NK cell development, sometimes in the absence of NK1.1, with CD94 invariably being expressed at two different levels. IL-4 differentially inhibited the expression of CD94 and Ly49 receptors, but had little or no effect on the expression of NKRP1 molecules. 相似文献
132.
133.
Proteins of Rous sarcoma virus 总被引:32,自引:0,他引:32
134.
135.
136.
Bowman E. M.; Brown V. J.; Kertzman C.; Schwarz U.; Robinson D. L. 《Journal of neurophysiology》1993,70(1):431-443
137.
Alexander Yu Donald Turbiville Fangling Xu Joseph W. Ray Allison D. Britt Pamela J. Lupo Sunil K. Jain Karen E. Shattuck Sally S. Robinson Jianli Dong 《American journal of medical genetics. Part A》2019,179(11):2178-2189
Duplications in the 22q11.2 region can cause 22q11.2 duplication syndrome and encompass a variety of phenotypes including developmental delays, facial abnormalities, cardiovascular defects, central nervous system delays, and other congenital abnormalities. However, the contribution of these contiguous duplicated regions to the clinical phenotypes has not been fully elucidated. In this study, we identified nine patients carrying different 22q11.2 microduplications detected by chromosomal microarray. Of these patients, seven pediatric patients presented with various clinical features including two neonate cases died shortly after birth, and two healthy adults. We examined region specific genotype–phenotype associations and found unpredictability associated with 22q11.2 duplications in these nine patients. 相似文献
138.
SV40 virus particles lack a psoralen-accessible origin and contain an altered nucleoprotein structure 总被引:1,自引:0,他引:1
The nucleoprotein structure of SV40 virions was examined by photolabeling purified virus with the radioactive psoralen derivative hydroxymethyltrimethylpsoralen (HMT). Unlike SV40 chromatin in situ, the viral origin region is not preferentially accessible to drug addition. The ratio of the distribution of radioactivity in the DNA restriction fragments of virion DNA to that of purified SV40 DNA demonstrates that the photoadducts are positioned similarly on the circular molecule in both samples. Virion purified from infected cells was also analyzed for the presence of an open region and found to exhibit the same pattern of [3H]HMT addition as mature extracellular virion. The nucleosome-free region detected at the SV40 replication origin in intracellular minichromosomes is not present in either population of intact virus particles. We also examined the level of drug addition obtained when purified virion or SV40-infected cells were treated with saturating doses of [3H]HMT. Marked differences in the plateau levels of bound drug indicate that an altered nucleoprotein structure exists in SV40 virions that does not protect the DNA from photoaddition to the same extent as do the nucleosomes of intracellular SV40 DNA. 相似文献
139.
Martineau M Jalali GR Barber KE Broadfield ZJ Cheung KL Lilleyman J Moorman AV Richards S Robinson HM Ross F Harrison CJ 《Genes, chromosomes & cancer》2005,43(1):54-71
This study was undertaken in order to compare the interphase and metaphase cytogenetics of 28 patients with ETV6/RUNX1 positive acute lymphoblastic leukemia, at diagnosis and relapse. The median time to relapse was 26 months. The significant fusion positive population heterogeneity revealed at interphase by a commercial probe for ETV6/RUNX1 fusion has not been described before. Six diagnostic samples had a single abnormal population; others had up to five each, which differed in the numbers of RUNX1 signals, and in the retention or loss of the second ETV6 signal. In contrast, the number of fusion signals was more constant. At relapse, there were fewer populations; the largest or unique clone was sometimes a re-emergence of a minor, diagnostic one, with a retained copy of ETV6 and the most RUNX1 signals. Abnormal, fusion negative clones were identified in bone marrow samples at extra-medullary relapse. Variant three or four-way translocations, which involved chromosomes 12 and 21, were prominent among the complex rearrangements revealed by metaphase FISH. The frequency of their occurrence at diagnosis and reappearance at relapse, sometimes accompanied by minor clonal evolution, was another new observation. Other recurrent cytogenetic features included a second copy of the fusion signal in six cases, partial duplication of the long arm of the X chromosome in two cases, and trisomy 10 in three cases. In comparing our data with previously reported cases, a picture is beginning to emerge of certain diagnostic features, which may provide circumstantial evidence of an increased risk of relapse. 相似文献
140.
After synthesis and folding, peptides and proteins undergo changes in charge and conformation through nonenzymatic deamidation of asparaginyl and glutaminyl residues. Each amide has a specific deamidation rate that is genetically determined by the sequence of residues immediately adjacent in the peptide chain and by secondary, tertiary, and quaternary structure. By means of experimentally verified computations, we have determined the deamidation rates of 49 Drosophila peptides and proteins. These rates demonstrate that deamidation provides molecular clocks that are suitable for the regulation of Drosophila aging, development, and other biochemical processes. We have also determined the rates of deamidation for 17,886 other proteins from a wide variety of organisms. The distribution function of these deamidation rates demonstrates the suitability of amide residues as biomolecular clocks. 相似文献